Q6P1X5 (TAF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 2

Alternative name(s):
150 kDa cofactor of initiator function
RNA polymerase II TBP-associated factor subunit B
TBP-associated factor 150 kDa
Transcription initiation factor TFIID 150 kDa subunit
Short name=TAF(II)150
Short name=TAFII-150
Short name=TAFII150

Gene names

Name:	TAF2
Synonyms:	CIF150, TAF2B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1199 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter. Ref.1 Ref.2
Subunit structure	Component of transcription factor TFIID which is composed of TBP and a number of TBP-associated factors. Interacts with TAF2C1. Component of the TFTC-HAT complex. Ref.1
Subcellular location	Nucleus Ref.2.
Tissue specificity	Expressed in all tissues tested. Ref.2
Miscellaneous	Ref.1 was unable to show an association between TAF2 and TFIID.
Sequence similarities	Belongs to the TAF2 family.
Sequence caution	The sequence AAC68502.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH64830.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G2/M transition of mitotic cell cycle Inferred from mutant phenotype. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: UniProtKB transcription initiation from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	transcription factor TFIID complex Inferred from direct assay Ref.2Ref.1. Source: UniProtKB transcription factor TFTC complex Inferred from direct assay. Source: UniProtKB
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.1. Source: UniProtKB transcription regulatory region DNA binding Inferred from mutant phenotype. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1199	1199	Transcription initiation factor TFIID subunit 2		PRO_0000252424
Regions
	Compositional bias	600 – 603	4	Poly-Lys
	Compositional bias	1143 – 1171	29	His-rich
	Compositional bias	1155 – 1178	24	Lys-rich
Amino acid modifications
	Modified residue	1194	1	Phosphoserine Ref.8 Ref.9 Ref.11
	Modified residue	1196	1	Phosphoserine Ref.8 Ref.9 Ref.10 Ref.11
	Modified residue	1198	1	Phosphoserine Ref.8 Ref.9 Ref.11
Natural variations
	Natural variant	8	1	P → L. Corresponds to variant rs17818842 [ dbSNP | Ensembl ].		VAR_027854
	Natural variant	447	1	S → T. Ref.1 Ref.2 Ref.3 Ref.6 Corresponds to variant rs9297605 [ dbSNP | Ensembl ].		VAR_027855
	Natural variant	686	1	E → K. Corresponds to variant rs34154779 [ dbSNP | Ensembl ].		VAR_057263
	Natural variant	1122	1	S → N. Corresponds to variant rs956749 [ dbSNP | Ensembl ].		VAR_027856
	Natural variant	1139	1	T → A. Corresponds to variant rs956748 [ dbSNP | Ensembl ].		VAR_027857
Experimental info
	Sequence conflict	1	1	M → R Ref.2
	Sequence conflict	41	1	Q → H in AAC02966. Ref.1
	Sequence conflict	131	1	P → L in AAH47732. Ref.6
	Sequence conflict	158	1	G → C in AAH64830. Ref.6
	Sequence conflict	604	1	I → K in AAH35673. Ref.6
	Sequence conflict	604	1	I → K in AAH47732. Ref.6
	Sequence conflict	785	1	R → G in AAC68502. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6P1X5 [UniParc]. Last modified April 17, 2007. Version 3. Checksum: 169DA4A3878CFD59	FASTA	1,199	136,971
	10 20 30 40 50 60 MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL 70 80 90 100 110 120 NRIKLNSKQC RIYRVRINDL EAAFIYNDPT LEVCHSESKQ RNLNYFSNAY AAAVSAVDPD 130 140 150 160 170 180 AGNGELCIKV PSELWKHVDE LKVLKIHINF SLDQPKGGLH FVVPSVEGSM AERGAHVFSC 190 200 210 220 230 240 GYQNSTRFWF PCVDSYSELC TWKLEFTVDA AMVAVSNGDL VETVYTHDMR KKTFHYMLTI 250 260 270 280 290 300 PTAASNISLA IGPFEILVDP YMHEVTHFCL PQLLPLLKHT TSYLHEVFEF YEEILTCRYP 310 320 330 340 350 360 YSCFKTVFID EAYVEVAAYA SMSIFSTNLL HSAMIIDETP LTRRCLAQSL AQQFFGCFIS 370 380 390 400 410 420 RMSWSDEWVL KGISGYIYGL WMKKTFGVNE YRHWIKEELD KIVAYELKTG GVLLHPIFGG 430 440 450 460 470 480 GKEKDNPASH LHFSIKHPHT LSWEYYSMFQ CKAHLVMRLI ENRISMEFML QVFNKLLSLA 490 500 510 520 530 540 STASSQKFQS HMWSQMLVST SGFLKSISNV SGKDIQPLIK QWVDQSGVVK FYGSFAFNRK 550 560 570 580 590 600 RNVLELEIKQ DYTSPGTQKY VGPLKVTVQE LDGSFNHTLQ IEENSLKHDI PCHSKSRRNK 610 620 630 640 650 660 KKKIPLMNGE EVDMDLSAMD ADSPLLWIRI DPDMSVLRKV EFEQADFMWQ YQLRYERDVV 670 680 690 700 710 720 AQQESILALE KFPTPASRLA LTDILEQEQC FYRVRMSACF CLAKIANSMV STWTGPPAMK 730 740 750 760 770 780 SLFTRMFCCK SCPNIVKTNN FMSFQSYFLQ KTMPVAMALL RDVHNLCPKE VLTFILDLIK 790 800 810 820 830 840 YNDNRKNKFS DNYYRAEMID ALANSVTPAV SVNNEVRTLD NLNPDVRLIL EEITRFLNME 850 860 870 880 890 900 KLLPSYRHTI TVSCLRAIRV LQKNGHVPSD PALFKSYAEY GHFVDIRIAA LEAVVDYTKV 910 920 930 940 950 960 DRSYEELQWL LNMIQNDPVP YVRHKILNML TKNPPFTKNM ESPLCNEALV DQLWKLMNSG 970 980 990 1000 1010 1020 TSHDWRLRCG AVDLYFTLFG LSRPSCLPLP ELGLVLNLKE KKAVLNPTII PESVAGNQEA 1030 1040 1050 1060 1070 1080 ANNPSSHPQL VGFQNPFSSS QDEEEIDMDT VHDSQAFISH HLNMLERPST PGLSKYRPAS 1090 1100 1110 1120 1130 1140 SRSALIPQHS AGCDSTPTTK PQWSLELARK GTGKEQAPLE MSMHPAASAP LSVFTKESTA 1150 1160 1170 1180 1190 SKHSDHHHHH HHEHKKKKKK HKHKHKHKHK HDSKEKDKEP FTFSSPASGR SIRSPSLSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CIF150, a human cofactor for transcription factor IID-dependent initiator function." Kaufmann J., Ahrens K., Koop R., Smale S.T., Muller R. Mol. Cell. Biol. 18:233-239(1998) [PubMed: 9418870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-447, FUNCTION, INTERACTION WITH TAF2C1.
[2]	"Novel cofactors and TFIIA mediate functional core promoter selectivity by the human TAFII150-containing TFIID complex." Martinez E., Ge H., Tao Y., Yuan C.-X., Palhan V., Roeder R.G. Mol. Cell. Biol. 18:6571-6583(1998) [PubMed: 9774672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-447, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE TFIID COMPLEX, TISSUE SPECIFICITY. Tissue: Cervix carcinoma.
[3]	"Human TBP-associated factor (TAFII150)." Guermah M., Roeder R.G.R. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-447. Tissue: Cervix carcinoma.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Trachea.
[5]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed: 16421571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-604, VARIANT THR-447. Tissue: Duodenum and Uterus.
[7]	"Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis." Cavusoglu N., Brand M., Tora L., van Dorsselaer A. Proteomics 3:217-223(2003) [PubMed: 12601814] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX, MASS SPECTROMETRY.
[8]	"Global phosphoproteome of HT-29 human colon adenocarcinoma cells." Kim J.-E., Tannenbaum S.R., White F.M. J. Proteome Res. 4:1339-1346(2005) [PubMed: 16083285] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1194; SER-1196 AND SER-1198, MASS SPECTROMETRY. Tissue: Colon adenocarcinoma.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1194; SER-1196 AND SER-1198, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1196, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1194; SER-1196 AND SER-1198, MASS SPECTROMETRY. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF026445 mRNA. Translation: AAC02966.1. AF040701 mRNA. Translation: AAC68502.1. Different initiation. AF057694 mRNA. Translation: AAC13540.1. AK316592 mRNA. Translation: BAG38179.1. AC107960 Genomic DNA. No translation available. AC021945 Genomic DNA. No translation available. BC035673 mRNA. Translation: AAH35673.1. BC047732 mRNA. Translation: AAH47732.1. BC064830 mRNA. Translation: AAH64830.1. Sequence problems.
IPI	IPI00328144.
RefSeq	NP_003175.1. NM_003184.3.
UniGene	Hs.122752.
3D structure databases
ProteinModelPortal	Q6P1X5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6P1X5. 4 interactions.
STRING	Q6P1X5.
Protein family/group databases
MEROPS	M01.972.
PTM databases
PhosphoSite	Q6P1X5.
Polymorphism databases
DMDM	145559533.
Proteomic databases
PRIDE	Q6P1X5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378164; ENSP00000367406; ENSG00000064313.
GeneID	6873.
KEGG	hsa:6873.
Organism-specific databases
CTD	6873.
GeneCards	GC08M120812.
HGNC	HGNC:11536. TAF2.
MIM	604912. gene.
neXtProt	NX_Q6P1X5.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13355.
GeneTree	ENSGT00390000000420.
HOVERGEN	HBG094022.
InParanoid	Q6P1X5.
OMA	CHSESKQ.
OrthoDB	EOG46HG8V.
PhylomeDB	Q6P1X5.
Gene expression databases
ArrayExpress	Q6P1X5.
Bgee	Q6P1X5.
CleanEx	HS_TAF2.
Genevestigator	Q6P1X5.
GermOnline	ENSG00000064313. Homo sapiens.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR014782. Peptidase_M1_N. [Graphical view]
KO	K03128.
Pfam	PF01433. Peptidase_M1. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	26833.
SOURCE	Search...

Entry information

Entry name

TAF2_HUMAN

Accession

Primary (citable) accession number: Q6P1X5
Secondary accession number(s): B2RE82 , O43487, O43604, O60668, Q86WW7, Q8IWK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	April 17, 2007
Last modified:	January 25, 2012
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.