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Q6P1X5 (TAF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription initiation factor TFIID subunit 2
Alternative name(s):
150 kDa cofactor of initiator function
RNA polymerase II TBP-associated factor subunit B
TBP-associated factor 150 kDa
Transcription initiation factor TFIID 150 kDa subunit
Short name=TAF(II)150
Short name=TAFII-150
Short name=TAFII150
Gene names
Name:TAF2
Synonyms:CIF150, TAF2B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1199 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter. Ref.1 Ref.2

Subunit structure

Component of transcription factor TFIID which is composed of TBP and a number of TBP-associated factors. Interacts with TAF2C1. Component of the TFTC-HAT complex. Ref.1 Ref.2 Ref.7

Subcellular location

Nucleus Ref.2.

Tissue specificity

Expressed in all tissues tested. Ref.2

Miscellaneous

Ref.1 was unable to show an association between TAF2 and TFIID.

Sequence similarities

Belongs to the TAF2 family.

Sequence caution

The sequence AAC68502.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH64830.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11991199Transcription initiation factor TFIID subunit 2
PRO_0000252424

Regions

Compositional bias600 – 6034Poly-Lys
Compositional bias1143 – 117129His-rich
Compositional bias1155 – 117824Lys-rich

Amino acid modifications

Modified residue11851Phosphoserine Ref.9
Modified residue11881Phosphoserine Ref.9
Modified residue11941Phosphoserine Ref.8
Modified residue11961Phosphoserine Ref.8
Modified residue11981Phosphoserine Ref.8

Natural variations

Natural variant81P → L.
Corresponds to variant rs17818842 [ dbSNP | Ensembl ].
VAR_027854
Natural variant4471S → T. Ref.1 Ref.2 Ref.3 Ref.6
Corresponds to variant rs9297605 [ dbSNP | Ensembl ].
VAR_027855
Natural variant6861E → K.
Corresponds to variant rs34154779 [ dbSNP | Ensembl ].
VAR_057263
Natural variant11221S → N.
Corresponds to variant rs956749 [ dbSNP | Ensembl ].
VAR_027856
Natural variant11391T → A.
Corresponds to variant rs956748 [ dbSNP | Ensembl ].
VAR_027857

Experimental info

Sequence conflict11M → R Ref.2
Sequence conflict411Q → H in AAC02966. Ref.1
Sequence conflict1311P → L in AAH47732. Ref.6
Sequence conflict1581G → C in AAH64830. Ref.6
Sequence conflict6041I → K in AAH35673. Ref.6
Sequence conflict6041I → K in AAH47732. Ref.6
Sequence conflict7851R → G in AAC68502. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q6P1X5 [UniParc].

Last modified April 17, 2007. Version 3.
Checksum: 169DA4A3878CFD59

FASTA1,199136,971
        10         20         30         40         50         60 
MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL 

        70         80         90        100        110        120 
NRIKLNSKQC RIYRVRINDL EAAFIYNDPT LEVCHSESKQ RNLNYFSNAY AAAVSAVDPD 

       130        140        150        160        170        180 
AGNGELCIKV PSELWKHVDE LKVLKIHINF SLDQPKGGLH FVVPSVEGSM AERGAHVFSC 

       190        200        210        220        230        240 
GYQNSTRFWF PCVDSYSELC TWKLEFTVDA AMVAVSNGDL VETVYTHDMR KKTFHYMLTI 

       250        260        270        280        290        300 
PTAASNISLA IGPFEILVDP YMHEVTHFCL PQLLPLLKHT TSYLHEVFEF YEEILTCRYP 

       310        320        330        340        350        360 
YSCFKTVFID EAYVEVAAYA SMSIFSTNLL HSAMIIDETP LTRRCLAQSL AQQFFGCFIS 

       370        380        390        400        410        420 
RMSWSDEWVL KGISGYIYGL WMKKTFGVNE YRHWIKEELD KIVAYELKTG GVLLHPIFGG 

       430        440        450        460        470        480 
GKEKDNPASH LHFSIKHPHT LSWEYYSMFQ CKAHLVMRLI ENRISMEFML QVFNKLLSLA 

       490        500        510        520        530        540 
STASSQKFQS HMWSQMLVST SGFLKSISNV SGKDIQPLIK QWVDQSGVVK FYGSFAFNRK 

       550        560        570        580        590        600 
RNVLELEIKQ DYTSPGTQKY VGPLKVTVQE LDGSFNHTLQ IEENSLKHDI PCHSKSRRNK 

       610        620        630        640        650        660 
KKKIPLMNGE EVDMDLSAMD ADSPLLWIRI DPDMSVLRKV EFEQADFMWQ YQLRYERDVV 

       670        680        690        700        710        720 
AQQESILALE KFPTPASRLA LTDILEQEQC FYRVRMSACF CLAKIANSMV STWTGPPAMK 

       730        740        750        760        770        780 
SLFTRMFCCK SCPNIVKTNN FMSFQSYFLQ KTMPVAMALL RDVHNLCPKE VLTFILDLIK 

       790        800        810        820        830        840 
YNDNRKNKFS DNYYRAEMID ALANSVTPAV SVNNEVRTLD NLNPDVRLIL EEITRFLNME 

       850        860        870        880        890        900 
KLLPSYRHTI TVSCLRAIRV LQKNGHVPSD PALFKSYAEY GHFVDIRIAA LEAVVDYTKV 

       910        920        930        940        950        960 
DRSYEELQWL LNMIQNDPVP YVRHKILNML TKNPPFTKNM ESPLCNEALV DQLWKLMNSG 

       970        980        990       1000       1010       1020 
TSHDWRLRCG AVDLYFTLFG LSRPSCLPLP ELGLVLNLKE KKAVLNPTII PESVAGNQEA 

      1030       1040       1050       1060       1070       1080 
ANNPSSHPQL VGFQNPFSSS QDEEEIDMDT VHDSQAFISH HLNMLERPST PGLSKYRPAS 

      1090       1100       1110       1120       1130       1140 
SRSALIPQHS AGCDSTPTTK PQWSLELARK GTGKEQAPLE MSMHPAASAP LSVFTKESTA 

      1150       1160       1170       1180       1190 
SKHSDHHHHH HHEHKKKKKK HKHKHKHKHK HDSKEKDKEP FTFSSPASGR SIRSPSLSD 

« Hide

References

« Hide 'large scale' references
[1]"CIF150, a human cofactor for transcription factor IID-dependent initiator function."
Kaufmann J., Ahrens K., Koop R., Smale S.T., Muller R.
Mol. Cell. Biol. 18:233-239(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-447, FUNCTION, INTERACTION WITH TAF2C1.
[2]"Novel cofactors and TFIIA mediate functional core promoter selectivity by the human TAFII150-containing TFIID complex."
Martinez E., Ge H., Tao Y., Yuan C.-X., Palhan V., Roeder R.G.
Mol. Cell. Biol. 18:6571-6583(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-447, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE TFIID COMPLEX, TISSUE SPECIFICITY.
Tissue: Cervix carcinoma.
[3]"Human TBP-associated factor (TAFII150)."
Guermah M., Roeder R.G.R.
Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT THR-447.
Tissue: Cervix carcinoma.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Trachea.
[5]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-604, VARIANT THR-447.
Tissue: Duodenum and Uterus.
[7]"Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis."
Cavusoglu N., Brand M., Tora L., van Dorsselaer A.
Proteomics 3:217-223(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1194; SER-1196 AND SER-1198, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1185 AND SER-1188, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF026445 mRNA. Translation: AAC02966.1.
AF040701 mRNA. Translation: AAC68502.1. Different initiation.
AF057694 mRNA. Translation: AAC13540.1.
AK316592 mRNA. Translation: BAG38179.1.
AC107960 Genomic DNA. No translation available.
AC021945 Genomic DNA. No translation available.
BC035673 mRNA. Translation: AAH35673.1.
BC047732 mRNA. Translation: AAH47732.1.
BC064830 mRNA. Translation: AAH64830.1. Sequence problems.
RefSeqNP_003175.1. NM_003184.3.
UniGeneHs.122752.

3D structure databases

ProteinModelPortalQ6P1X5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112736. 19 interactions.
IntActQ6P1X5. 5 interactions.
STRING9606.ENSP00000367406.

Protein family/group databases

MEROPSM01.972.

PTM databases

PhosphoSiteQ6P1X5.

Polymorphism databases

DMDM145559533.

Proteomic databases

PaxDbQ6P1X5.
PRIDEQ6P1X5.

Protocols and materials databases

DNASU6873.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000378164; ENSP00000367406; ENSG00000064313.
GeneID6873.
KEGGhsa:6873.
UCSCuc003you.3. human.

Organism-specific databases

CTD6873.
GeneCardsGC08M120812.
HGNCHGNC:11536. TAF2.
MIM604912. gene.
neXtProtNX_Q6P1X5.
PharmGKBPA36311.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0308.
HOVERGENHBG094022.
InParanoidQ6P1X5.
KOK03128.
OMAPCVDSFA.
OrthoDBEOG7R2BHS.
PhylomeDBQ6P1X5.
TreeFamTF315208.

Gene expression databases

ArrayExpressQ6P1X5.
BgeeQ6P1X5.
CleanExHS_TAF2.
GenevestigatorQ6P1X5.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR014782. Peptidase_M1_N.
[Graphical view]
PfamPF01433. Peptidase_M1. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
ProtoNetSearch...

Other

ChiTaRSTAF2. human.
GeneWikiTAF2.
GenomeRNAi6873.
NextBio26833.
PROQ6P1X5.
SOURCESearch...

Entry information

Entry nameTAF2_HUMAN
AccessionPrimary (citable) accession number: Q6P1X5
Secondary accession number(s): B2RE82 expand/collapse secondary AC list , O43487, O43604, O60668, Q86WW7, Q8IWK4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: April 17, 2007
Last modified: April 16, 2014
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM