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Q6P1J6 (PLB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospholipase B1, membrane-associated

Short name=Phospholipase B
Short name=hPLB
Alternative name(s):
Phospholipase B/lipase
Short name=PLB/LIP

Including the following 2 domains:

  1. Phospholipase A2
    EC=3.1.1.4
  2. Lysophospholipase
    EC=3.1.1.5
Gene names
Name:PLB1
Synonyms:PLB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1458 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids By similarity.

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulation

Inhibited by diisopropyl fluorophosphate By similarity.

Subcellular location

Apical cell membrane; Single-pass type I membrane protein By similarity. Note: Present in the intestinal brush border membranes By similarity.

Tissue specificity

Expressed in the epidermis (at protein level). Ref.5

Domain

Repeat 2 contains the catalytic domain By similarity.

Post-translational modification

Undergoes proteolytic cleavage in the ileum By similarity.

Sequence similarities

Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily.

Sequence caution

The sequence BAB70920.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6P1J6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6P1J6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-312: Missing.
     774-800: SAGGDGSLENVTTLPNILREFNRNLTG → RESKPGFLSDSWVSKSNRKCTRKAPNP
     801-1458: Missing.
Isoform 3 (identifier: Q6P1J6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     184-184: Q → QQAPSLSTVLLS
     523-544: Missing.
Isoform 4 (identifier: Q6P1J6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     750-760: AGNGIGSKPDD → VRTLGPQVVWG
     761-1458: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q6P1J6-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1035: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 14581437Phospholipase B1, membrane-associated
PRO_0000324383

Regions

Topological domain22 – 14171396Extracellular Potential
Transmembrane1418 – 143821Helical; Potential
Topological domain1439 – 145820Cytoplasmic Potential
Repeat39 – 3473091
Repeat362 – 7073462
Repeat708 – 10543473
Repeat1064 – 14023394
Region39 – 140213644 X 308-326 AA approximate repeats
Region1403 – 144543Necessary for membrane localization By similarity

Sites

Active site4001Nucleophile By similarity
Active site13461 By similarity
Active site13491 By similarity
Binding site4741Substrate; via amide nitrogen By similarity
Binding site5131Substrate By similarity

Amino acid modifications

Glycosylation2401N-linked (GlcNAc...) Potential
Glycosylation4931N-linked (GlcNAc...) Potential
Glycosylation5291N-linked (GlcNAc...) Potential
Glycosylation7971N-linked (GlcNAc...) Potential
Glycosylation10551N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 10351035Missing in isoform 5.
VSP_032225
Alternative sequence1 – 312312Missing in isoform 2.
VSP_032226
Alternative sequence1841Q → QQAPSLSTVLLS in isoform 3.
VSP_032227
Alternative sequence523 – 54422Missing in isoform 3.
VSP_032228
Alternative sequence750 – 76011AGNGIGSKPDD → VRTLGPQVVWG in isoform 4.
VSP_032229
Alternative sequence761 – 1458698Missing in isoform 4.
VSP_032230
Alternative sequence774 – 80027SAGGD…RNLTG → RESKPGFLSDSWVSKSNRKC TRKAPNP in isoform 2.
VSP_032231
Alternative sequence801 – 1458658Missing in isoform 2.
VSP_032232
Natural variant2121V → L.
Corresponds to variant rs6753929 [ dbSNP | Ensembl ].
VAR_039793
Natural variant7081M → V.
Corresponds to variant rs11681826 [ dbSNP | Ensembl ].
VAR_039794
Natural variant8211G → R.
Corresponds to variant rs10201128 [ dbSNP | Ensembl ].
VAR_039795
Natural variant8791H → D.
Corresponds to variant rs7601771 [ dbSNP | Ensembl ].
VAR_039796
Natural variant9871A → V.
Corresponds to variant rs34289907 [ dbSNP | Ensembl ].
VAR_061358
Natural variant13181A → V.
Corresponds to variant rs2199619 [ dbSNP | Ensembl ].
VAR_039797

Experimental info

Sequence conflict4261W → Y in BAB70920. Ref.4
Sequence conflict11861V → L in AAH42674. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 0B260E1489D8137B

FASTA1,458163,081
        10         20         30         40         50         60 
MGLRPGIFLL ELLLLLGQGT PQIHTSPRKS TLEGQLWPET LKNSPFPCNP NKLGVNMPSK 

        70         80         90        100        110        120 
SVHSLKPSDI KFVAAIGNLE IPPDPGTGDL EKQDWTERPQ QVCMGVMTVL SDIIRYFSPS 

       130        140        150        160        170        180 
VPMPVCHTGK RVIPHDGAED LWIQAQELVR NMKENLQLDF QFDWKLINVF FSNASQCYLC 

       190        200        210        220        230        240 
PSAQQNGLAA GGVDELMGVL DYLQQEVPRA FVNLVDLSEV AEVSRQYHGT WLSPAPEPCN 

       250        260        270        280        290        300 
CSEETTRLAK VVMQWSYQEA WNSLLASSRY SEQESFTVVF QPFFYETTPS LHSEDPRLQD 

       310        320        330        340        350        360 
STTLAWHLWN RMMEPAGEKD EPLSVKHGRP MKCPSQESPY LFSYRNSNYL TRLQKPQDKL 

       370        380        390        400        410        420 
EVREGAEIRC PDKDPSDTVP TSVHRLKPAD INVIGALGDS LTAGNGAGST PGNVLDVLTQ 

       430        440        450        460        470        480 
YRGLSWSVGG DENIGTVTTL ANILREFNPS LKGFSVGTGK ETSPNAFLNQ AVAGGRAEDL 

       490        500        510        520        530        540 
PVQARRLVDL MKNDTRIHFQ EDWKIITLFI GGNDLCDFCN DLVHYSPQNF TDNIGKALDI 

       550        560        570        580        590        600 
LHAEVPRAFV NLVTVLEIVN LRELYQEKKV YCPRMILRSL CPCVLKFDDN STELATLIEF 

       610        620        630        640        650        660 
NKKFQEKTHQ LIESGRYDTR EDFTVVVQPF FENVDMPKTS EGLPDNSFFA PDCFHFSSKS 

       670        680        690        700        710        720 
HSRAASALWN NMLEPVGQKT TRHKFENKIN ITCPNQVQPF LRTYKNSMQG HGTWLPCRDR 

       730        740        750        760        770        780 
APSALHPTSV HALRPADIQV VAALGDSLTA GNGIGSKPDD LPDVTTQYRG LSYSAGGDGS 

       790        800        810        820        830        840 
LENVTTLPNI LREFNRNLTG YAVGTGDAND TNAFLNQAVP GAKAEDLMSQ VQTLMQKMKD 

       850        860        870        880        890        900 
DHRVNFHEDW KVITVLIGGS DLCDYCTDSN LYSAANFVHH LRNALDVLHR EVPRVLVNLV 

       910        920        930        940        950        960 
DFLNPTIMRQ VFLGNPDKCP VQQASVLCNC VLTLRENSQE LARLEAFSRA YRSSMRELVG 

       970        980        990       1000       1010       1020 
SGRYDTQEDF SVVLQPFFQN IQLPVLADGL PDTSFFAPDC IHPNQKFHSQ LARALWTNML 

      1030       1040       1050       1060       1070       1080 
EPLGSKTETL DLRAEMPITC PTQNEPFLRT PRNSNYTYPI KPAIENWGSD FLCTEWKASN 

      1090       1100       1110       1120       1130       1140 
SVPTSVHQLR PADIKVVAAL GDSLTTAVGA RPNNSSDLPT SWRGLSWSIG GDGNLETHTT 

      1150       1160       1170       1180       1190       1200 
LPNILKKFNP YLLGFSTSTW EGTAGLNVAA EGARARDMPA QAWDLVERMK NSPDINLEKD 

      1210       1220       1230       1240       1250       1260 
WKLVTLFIGV NDLCHYCENP EAHLATEYVQ HIQQALDILS EELPRAFVNV VEVMELASLY 

      1270       1280       1290       1300       1310       1320 
QGQGGKCAML AAQNNCTCLR HSQSSLEKQE LKKVNWNLQH GISSFSYWHQ YTQREDFAVV 

      1330       1340       1350       1360       1370       1380 
VQPFFQNTLT PLNERGDTDL TFFSEDCFHF SDRGHAEMAI ALWNNMLEPV GRKTTSNNFT 

      1390       1400       1410       1420       1430       1440 
HSRAKLKCPS PESPYLYTLR NSRLLPDQAE EAPEVLYWAV PVAAGVGLVV GIIGTVVWRC 

      1450 
RRGGRREDPP MSLRTVAL 

« Hide

Isoform 2 [UniParc].

Checksum: 6BE12FF5CB0AC9C5
Show »

FASTA48854,341
Isoform 3 [UniParc].

Checksum: 463E147644AF72B7
Show »

FASTA1,447161,713
Isoform 4 [UniParc].

Checksum: 6910E87C50726761
Show »

FASTA76085,191
Isoform 5 [UniParc].

Checksum: A66F32D49AC1A20C
Show »

FASTA42347,341

References

« Hide 'large scale' references
[1]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1458 (ISOFORM 3).
Tissue: Brain and Skin.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 426-1458 (ISOFORM 4).
Tissue: Brain.
[5]"Human epidermis is a novel site of phospholipase B expression."
Maury E., Prevost M.-C., Nauze M., Redoules D., Tarroux R., Charveron M., Salles J.-P., Perret B., Chap H., Gassama-Diagne A.
Biochem. Biophys. Res. Commun. 295:362-369(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC074011 Genomic DNA. Translation: AAY24081.1.
AC074011 Genomic DNA. Translation: AAY24082.1.
AC093164 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00532.1.
CH471053 Genomic DNA. Translation: EAX00533.1.
BC042674 mRNA. Translation: AAH42674.1.
BC065041 mRNA. Translation: AAH65041.1.
BC153864 mRNA. Translation: AAI53865.1.
AK055428 mRNA. Translation: BAB70920.1. Different initiation.
RefSeqNP_001164056.1. NM_001170585.1.
NP_694566.4. NM_153021.4.
UniGeneHs.444933.

3D structure databases

ProteinModelPortalQ6P1J6.
SMRQ6P1J6. Positions 163-212, 468-550, 647-673, 849-897, 992-1017, 1202-1248.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127341. 1 interaction.
STRING9606.ENSP00000330442.

PTM databases

PhosphoSiteQ6P1J6.

Proteomic databases

PaxDbQ6P1J6.
PRIDEQ6P1J6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000327757; ENSP00000330442; ENSG00000163803. [Q6P1J6-1]
ENST00000329020; ENSP00000330729; ENSG00000163803. [Q6P1J6-2]
ENST00000422425; ENSP00000416440; ENSG00000163803. [Q6P1J6-3]
ENST00000541605; ENSP00000437426; ENSG00000163803. [Q6P1J6-5]
GeneID151056.
KEGGhsa:151056.
UCSCuc002rmb.2. human. [Q6P1J6-1]
uc002rmc.3. human. [Q6P1J6-2]
uc002rme.2. human. [Q6P1J6-5]
uc010ezj.2. human. [Q6P1J6-3]

Organism-specific databases

CTD151056.
GeneCardsGC02P028732.
H-InvDBHIX0024210.
HGNCHGNC:30041. PLB1.
HPAHPA014045.
MIM610179. gene.
neXtProtNX_Q6P1J6.
PharmGKBPA134891309.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG311176.
HOGENOMHOG000115574.
HOVERGENHBG108263.
InParanoidQ6P1J6.
KOK14621.
OMACPDKDPS.
OrthoDBEOG7MPRD0.
PhylomeDBQ6P1J6.
TreeFamTF314942.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_111217. Metabolism.
REACT_116125. Disease.

Gene expression databases

ArrayExpressQ6P1J6.
BgeeQ6P1J6.
CleanExHS_PLB1.
GenevestigatorQ6P1J6.

Family and domain databases

Gene3D3.40.50.1110. 5 hits.
InterProIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi151056.
NextBio86583.
PROQ6P1J6.
SOURCESearch...

Entry information

Entry namePLB1_HUMAN
AccessionPrimary (citable) accession number: Q6P1J6
Secondary accession number(s): A8KAX2 expand/collapse secondary AC list , Q53S03, Q8IUP7, Q96DP9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM