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Protein

Phospholipase B1, membrane-associated

Gene

PLB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by diisopropyl fluorophosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei400NucleophileBy similarity1
Binding sitei474Substrate; via amide nitrogenBy similarity1
Binding sitei513SubstrateBy similarity1
Active sitei1346By similarity1
Active sitei1349By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciZFISH:HS08939-MONOMER.
ReactomeiR-HSA-1482788. Acyl chain remodelling of PC.
R-HSA-975634. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Short name:
hPLB
Alternative name(s):
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLB1
Synonyms:PLB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:30041. PLB1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 1417ExtracellularSequence analysisAdd BLAST1396
Transmembranei1418 – 1438HelicalSequence analysisAdd BLAST21
Topological domaini1439 – 1458CytoplasmicSequence analysisAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi151056.
OpenTargetsiENSG00000163803.
PharmGKBiPA134891309.

Polymorphism and mutation databases

BioMutaiPLB1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000032438322 – 1458Phospholipase B1, membrane-associatedAdd BLAST1437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Glycosylationi493N-linked (GlcNAc...)Sequence analysis1
Glycosylationi529N-linked (GlcNAc...)Sequence analysis1
Glycosylationi797N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1055N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6P1J6.
PeptideAtlasiQ6P1J6.
PRIDEiQ6P1J6.

PTM databases

iPTMnetiQ6P1J6.
PhosphoSitePlusiQ6P1J6.

Expressioni

Tissue specificityi

Expressed in the epidermis (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000163803.
CleanExiHS_PLB1.
ExpressionAtlasiQ6P1J6. baseline and differential.
GenevisibleiQ6P1J6. HS.

Organism-specific databases

HPAiHPA014045.

Interactioni

Protein-protein interaction databases

BioGridi127341. 12 interactors.
IntActiQ6P1J6. 3 interactors.
STRINGi9606.ENSP00000330442.

Structurei

3D structure databases

ProteinModelPortaliQ6P1J6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati39 – 3471Add BLAST309
Repeati362 – 7072Add BLAST346
Repeati708 – 10543Add BLAST347
Repeati1064 – 14024Add BLAST339

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 14024 X 308-326 AA approximate repeatsAdd BLAST1364
Regioni1403 – 1445Necessary for membrane localizationBy similarityAdd BLAST43

Domaini

Repeat 2 contains the catalytic domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3670. Eukaryota.
ENOG4111FH4. LUCA.
GeneTreeiENSGT00530000063883.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiQ6P1J6.
KOiK14621.
OMAiNDLCHYC.
OrthoDBiEOG091G00VL.
PhylomeDBiQ6P1J6.
TreeFamiTF314942.

Family and domain databases

Gene3Di3.40.50.1110. 5 hits.
InterProiIPR001087. GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 7 hits.
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P1J6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLRPGIFLL ELLLLLGQGT PQIHTSPRKS TLEGQLWPET LKNSPFPCNP
60 70 80 90 100
NKLGVNMPSK SVHSLKPSDI KFVAAIGNLE IPPDPGTGDL EKQDWTERPQ
110 120 130 140 150
QVCMGVMTVL SDIIRYFSPS VPMPVCHTGK RVIPHDGAED LWIQAQELVR
160 170 180 190 200
NMKENLQLDF QFDWKLINVF FSNASQCYLC PSAQQNGLAA GGVDELMGVL
210 220 230 240 250
DYLQQEVPRA FVNLVDLSEV AEVSRQYHGT WLSPAPEPCN CSEETTRLAK
260 270 280 290 300
VVMQWSYQEA WNSLLASSRY SEQESFTVVF QPFFYETTPS LHSEDPRLQD
310 320 330 340 350
STTLAWHLWN RMMEPAGEKD EPLSVKHGRP MKCPSQESPY LFSYRNSNYL
360 370 380 390 400
TRLQKPQDKL EVREGAEIRC PDKDPSDTVP TSVHRLKPAD INVIGALGDS
410 420 430 440 450
LTAGNGAGST PGNVLDVLTQ YRGLSWSVGG DENIGTVTTL ANILREFNPS
460 470 480 490 500
LKGFSVGTGK ETSPNAFLNQ AVAGGRAEDL PVQARRLVDL MKNDTRIHFQ
510 520 530 540 550
EDWKIITLFI GGNDLCDFCN DLVHYSPQNF TDNIGKALDI LHAEVPRAFV
560 570 580 590 600
NLVTVLEIVN LRELYQEKKV YCPRMILRSL CPCVLKFDDN STELATLIEF
610 620 630 640 650
NKKFQEKTHQ LIESGRYDTR EDFTVVVQPF FENVDMPKTS EGLPDNSFFA
660 670 680 690 700
PDCFHFSSKS HSRAASALWN NMLEPVGQKT TRHKFENKIN ITCPNQVQPF
710 720 730 740 750
LRTYKNSMQG HGTWLPCRDR APSALHPTSV HALRPADIQV VAALGDSLTA
760 770 780 790 800
GNGIGSKPDD LPDVTTQYRG LSYSAGGDGS LENVTTLPNI LREFNRNLTG
810 820 830 840 850
YAVGTGDAND TNAFLNQAVP GAKAEDLMSQ VQTLMQKMKD DHRVNFHEDW
860 870 880 890 900
KVITVLIGGS DLCDYCTDSN LYSAANFVHH LRNALDVLHR EVPRVLVNLV
910 920 930 940 950
DFLNPTIMRQ VFLGNPDKCP VQQASVLCNC VLTLRENSQE LARLEAFSRA
960 970 980 990 1000
YRSSMRELVG SGRYDTQEDF SVVLQPFFQN IQLPVLADGL PDTSFFAPDC
1010 1020 1030 1040 1050
IHPNQKFHSQ LARALWTNML EPLGSKTETL DLRAEMPITC PTQNEPFLRT
1060 1070 1080 1090 1100
PRNSNYTYPI KPAIENWGSD FLCTEWKASN SVPTSVHQLR PADIKVVAAL
1110 1120 1130 1140 1150
GDSLTTAVGA RPNNSSDLPT SWRGLSWSIG GDGNLETHTT LPNILKKFNP
1160 1170 1180 1190 1200
YLLGFSTSTW EGTAGLNVAA EGARARDMPA QAWDLVERMK NSPDINLEKD
1210 1220 1230 1240 1250
WKLVTLFIGV NDLCHYCENP EAHLATEYVQ HIQQALDILS EELPRAFVNV
1260 1270 1280 1290 1300
VEVMELASLY QGQGGKCAML AAQNNCTCLR HSQSSLEKQE LKKVNWNLQH
1310 1320 1330 1340 1350
GISSFSYWHQ YTQREDFAVV VQPFFQNTLT PLNERGDTDL TFFSEDCFHF
1360 1370 1380 1390 1400
SDRGHAEMAI ALWNNMLEPV GRKTTSNNFT HSRAKLKCPS PESPYLYTLR
1410 1420 1430 1440 1450
NSRLLPDQAE EAPEVLYWAV PVAAGVGLVV GIIGTVVWRC RRGGRREDPP

MSLRTVAL
Length:1,458
Mass (Da):163,081
Last modified:May 18, 2010 - v3
Checksum:i0B260E1489D8137B
GO
Isoform 2 (identifier: Q6P1J6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-312: Missing.
     774-800: SAGGDGSLENVTTLPNILREFNRNLTG → RESKPGFLSDSWVSKSNRKCTRKAPNP
     801-1458: Missing.

Show »
Length:488
Mass (Da):54,341
Checksum:i6BE12FF5CB0AC9C5
GO
Isoform 3 (identifier: Q6P1J6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     184-184: Q → QQAPSLSTVLLS
     523-544: Missing.

Show »
Length:1,447
Mass (Da):161,713
Checksum:i463E147644AF72B7
GO
Isoform 4 (identifier: Q6P1J6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     750-760: AGNGIGSKPDD → VRTLGPQVVWG
     761-1458: Missing.

Note: No experimental confirmation available.
Show »
Length:760
Mass (Da):85,191
Checksum:i6910E87C50726761
GO
Isoform 5 (identifier: Q6P1J6-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1035: Missing.

Show »
Length:423
Mass (Da):47,341
Checksum:iA66F32D49AC1A20C
GO

Sequence cautioni

The sequence BAB70920 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti426W → Y in BAB70920 (PubMed:14702039).Curated1
Sequence conflicti1186V → L in AAH42674 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_039793212V → L.Corresponds to variant rs6753929dbSNPEnsembl.1
Natural variantiVAR_039794708M → V.Corresponds to variant rs11681826dbSNPEnsembl.1
Natural variantiVAR_039795821G → R.Corresponds to variant rs10201128dbSNPEnsembl.1
Natural variantiVAR_039796879H → D.Corresponds to variant rs7601771dbSNPEnsembl.1
Natural variantiVAR_061358987A → V.Corresponds to variant rs34289907dbSNPEnsembl.1
Natural variantiVAR_0397971318A → V.Corresponds to variant rs2199619dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0322251 – 1035Missing in isoform 5. 1 PublicationAdd BLAST1035
Alternative sequenceiVSP_0322261 – 312Missing in isoform 2. 1 PublicationAdd BLAST312
Alternative sequenceiVSP_032227184Q → QQAPSLSTVLLS in isoform 3. 1 Publication1
Alternative sequenceiVSP_032228523 – 544Missing in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_032229750 – 760AGNGIGSKPDD → VRTLGPQVVWG in isoform 4. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_032230761 – 1458Missing in isoform 4. 1 PublicationAdd BLAST698
Alternative sequenceiVSP_032231774 – 800SAGGD…RNLTG → RESKPGFLSDSWVSKSNRKC TRKAPNP in isoform 2. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_032232801 – 1458Missing in isoform 2. 1 PublicationAdd BLAST658

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC074011 Genomic DNA. Translation: AAY24081.1.
AC074011 Genomic DNA. Translation: AAY24082.1.
AC093164 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00532.1.
CH471053 Genomic DNA. Translation: EAX00533.1.
BC042674 mRNA. Translation: AAH42674.1.
BC065041 mRNA. Translation: AAH65041.1.
BC153864 mRNA. Translation: AAI53865.1.
AK055428 mRNA. Translation: BAB70920.1. Different initiation.
CCDSiCCDS33168.1. [Q6P1J6-1]
CCDS54340.1. [Q6P1J6-3]
RefSeqiNP_001164056.1. NM_001170585.1. [Q6P1J6-3]
NP_694566.4. NM_153021.4. [Q6P1J6-1]
XP_016858922.1. XM_017003433.1. [Q6P1J6-1]
UniGeneiHs.444933.

Genome annotation databases

EnsembliENST00000327757; ENSP00000330442; ENSG00000163803. [Q6P1J6-1]
ENST00000422425; ENSP00000416440; ENSG00000163803. [Q6P1J6-3]
GeneIDi151056.
KEGGihsa:151056.
UCSCiuc002rmb.3. human. [Q6P1J6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC074011 Genomic DNA. Translation: AAY24081.1.
AC074011 Genomic DNA. Translation: AAY24082.1.
AC093164 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00532.1.
CH471053 Genomic DNA. Translation: EAX00533.1.
BC042674 mRNA. Translation: AAH42674.1.
BC065041 mRNA. Translation: AAH65041.1.
BC153864 mRNA. Translation: AAI53865.1.
AK055428 mRNA. Translation: BAB70920.1. Different initiation.
CCDSiCCDS33168.1. [Q6P1J6-1]
CCDS54340.1. [Q6P1J6-3]
RefSeqiNP_001164056.1. NM_001170585.1. [Q6P1J6-3]
NP_694566.4. NM_153021.4. [Q6P1J6-1]
XP_016858922.1. XM_017003433.1. [Q6P1J6-1]
UniGeneiHs.444933.

3D structure databases

ProteinModelPortaliQ6P1J6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127341. 12 interactors.
IntActiQ6P1J6. 3 interactors.
STRINGi9606.ENSP00000330442.

PTM databases

iPTMnetiQ6P1J6.
PhosphoSitePlusiQ6P1J6.

Polymorphism and mutation databases

BioMutaiPLB1.

Proteomic databases

PaxDbiQ6P1J6.
PeptideAtlasiQ6P1J6.
PRIDEiQ6P1J6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327757; ENSP00000330442; ENSG00000163803. [Q6P1J6-1]
ENST00000422425; ENSP00000416440; ENSG00000163803. [Q6P1J6-3]
GeneIDi151056.
KEGGihsa:151056.
UCSCiuc002rmb.3. human. [Q6P1J6-1]

Organism-specific databases

CTDi151056.
DisGeNETi151056.
GeneCardsiPLB1.
H-InvDBHIX0024210.
HGNCiHGNC:30041. PLB1.
HPAiHPA014045.
MIMi610179. gene.
neXtProtiNX_Q6P1J6.
OpenTargetsiENSG00000163803.
PharmGKBiPA134891309.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3670. Eukaryota.
ENOG4111FH4. LUCA.
GeneTreeiENSGT00530000063883.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiQ6P1J6.
KOiK14621.
OMAiNDLCHYC.
OrthoDBiEOG091G00VL.
PhylomeDBiQ6P1J6.
TreeFamiTF314942.

Enzyme and pathway databases

BioCyciZFISH:HS08939-MONOMER.
ReactomeiR-HSA-1482788. Acyl chain remodelling of PC.
R-HSA-975634. Retinoid metabolism and transport.

Miscellaneous databases

GenomeRNAii151056.
PROiQ6P1J6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163803.
CleanExiHS_PLB1.
ExpressionAtlasiQ6P1J6. baseline and differential.
GenevisibleiQ6P1J6. HS.

Family and domain databases

Gene3Di3.40.50.1110. 5 hits.
InterProiIPR001087. GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 7 hits.
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLB1_HUMAN
AccessioniPrimary (citable) accession number: Q6P1J6
Secondary accession number(s): A8KAX2
, Q53S03, Q8IUP7, Q96DP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 18, 2010
Last modified: November 2, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.