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Q6P1D7

- SLX4_MOUSE

UniProt

Q6P1D7 - SLX4_MOUSE

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Protein

Structure-specific endonuclease subunit SLX4

Gene
Slx4, Btbd12
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks By similarity.

GO - Molecular functioni

  1. 3'-flap endonuclease activity Source: Ensembl
  2. 5'-flap endonuclease activity Source: Ensembl
  3. crossover junction endodeoxyribonuclease activity Source: Ensembl
  4. enzyme activator activity Source: Ensembl

GO - Biological processi

  1. DNA double-strand break processing involved in repair via single-strand annealing Source: Ensembl
  2. DNA replication Source: InterPro
  3. double-strand break repair via homologous recombination Source: Ensembl
  4. nucleotide-excision repair Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Structure-specific endonuclease subunit SLX4
Alternative name(s):
BTB/POZ domain-containing protein 12
Gene namesi
Name:Slx4
Synonyms:Btbd12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:106299. Slx4.

Subcellular locationi

Nucleus By similarity
Note: Localizes to sites of DNA dammage By similarity.

GO - Cellular componenti

  1. ERCC4-ERCC1 complex Source: Ensembl
  2. Holliday junction resolvase complex Source: Ensembl
  3. nuclear chromatin Source: Ensembl
  4. nuclear chromosome, telomeric region Source: Ensembl
  5. Slx1-Slx4 complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi202 – 2021P → T: Does not modify the functional properties of the protein. 1 Publication
Mutagenesisi602 – 6021E → K: Does not modify the functional properties of the protein. 1 Publication
Mutagenesisi1324 – 13241Q → W: Does not modify the functional properties of the protein. 1 Publication
Mutagenesisi1545 – 15451R → C: Does not modify the functional properties of the protein. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15651565Structure-specific endonuclease subunit SLX4PRO_0000383567Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei111 – 1111Phosphoserine By similarity
Modified residuei845 – 8451Phosphoserine By similarity
Modified residuei1254 – 12541Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6P1D7.
PRIDEiQ6P1D7.

PTM databases

PhosphoSiteiQ6P1D7.

Expressioni

Gene expression databases

ArrayExpressiQ6P1D7.
BgeeiQ6P1D7.
GenevestigatoriQ6P1D7.

Interactioni

Subunit structurei

Forms a heterodimer with SLX1A/GIYD1. Interacts with ERCC4; catalytic subunit of the ERCC4-ERCC1 endonuclease. Interacts with MUS81; catalytic subunit of the MUS81-EME1 endonuclease. Interacts with MSH2; component of the MSH2-MSH3 mismatch repair complex. Interacts with TERF2-TERF2IP. Interacts with PLK1 and SLX4IP By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ6P1D7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini506 – 57974BTBAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni499 – 15651067Interaction with PLK1 and TERF2-TERF2IP By similarityAdd
BLAST
Regioni1120 – 1413294Interaction with MUS81 By similarityAdd
BLAST
Regioni1406 – 1565160Interaction with SLX1 By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the SLX4 family.
Contains 1 BTB (POZ) domain.

Phylogenomic databases

eggNOGiNOG44550.
GeneTreeiENSGT00390000014091.
HOGENOMiHOG000095273.
InParanoidiQ6P1D7.
KOiK10484.
OMAiHKFVLYA.
OrthoDBiEOG7ZKSDH.
PhylomeDBiQ6P1D7.
TreeFamiTF106446.

Family and domain databases

Gene3Di3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR018574. Structure-sp_endonuc_su_Slx4.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF09494. Slx4. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P1D7-1 [UniParc]FASTAAdd to Basket

« Hide

MVPESAPNGN SQPLPSCFTT TGVPSPSKPR VSELVLQRMK QFKRADPERL     50
RHASEESPQK TALGDDVPRS PPEETVGENE YKLDATDSDA AMALALQQEF 100
RREEASSHHD SLEEKGLFFC QMCQKNLSAM NVTRREQHVN RCLDEAEKAQ 150
RPASPRIPDC PICGKPFLTT KSRISHLKQC AVRMEVGPQL LLQAVRLQTA 200
QPEVDGSPQV PSFSNNVGGL KRKGVTTKRE PRRRKVNKPE APSEDLLVAM 250
ALSRSEVEHC PVVPPLRLEN AFSEKIRLGA EKKSRKKRPP VCPPQLVTQD 300
SETTGRQIED RVAQLLSEEA ELSCTPPLLA SKISKEELEP AGWRARLPEG 350
KRNFLWELSA LTGAWAEESF YTVGLFPPIV SQCPSKEPQL PLELPKQGEP 400
SPRRPPASQS SLPVSHSPKI RLLSSSQRER QALQDLVDLA VEGLSSSPQP 450
GSRGVPTGLD LVPSSLPLTG FVLPCKKTLK KDDSASLSLG LLVTDFGAMV 500
NNPHLSDVQF QLDSGEVLYA HKFVLYARCP LLIQYVSTES FSSEEDGDLT 550
QRALLSDVSS EAAHAFLNYL YMADTDMPPS LVPDLRSLAL RFGVSDLVQL 600
CEQVPAVVDL EGEQPEETSE DCESRAETFL ELLRSVWVDN EEEVETLLKP 650
ELCEEERERV NEAEMEEIYE FAATQRKLLQ WGRAADPDGS TNPHGEDGAV 700
SEPSLAGVQS NRQLENTEHM ESSGLEKEEA LASWEQEGHS TPLQDQCPDW 750
AGKAEAQDAL GEATDDPSFC SRHRRGKECL PLHPNKAHGC KQPLPSNPRV 800
SSELSQITVD HEEQSDHVRE TQADMAQAPT PHSCSLVSQS SVDGSPSQSW 850
LHLYHTSHLS PSVSQSHSSI SRVASPRSLS PTTPTKQRRG SNIVTLRKDA 900
GHHRGQQSSP IAGHRNRGIL ISPAKSPPID LTQSVPEPLS PRAQDPLHFV 950
KKEDEVILLL DSDEELEHTK TESVSKDSPE GRKVPEFSPR SSELFSVIDV 1000
EEDHEHFQSP LKREAGLQHG EEGQLGNQSA LGCRDIPWLL CSQKTSLDED 1050
SATDTSWLVP ATPGVSRSRD CSSQTQIKSL KTRIPSDETA QQTPRPNLER 1100
RTMLETAQQF SVIMPHTQPI TLGAFDSGRQ AYRSPSHPYP RHHRLSSSQP 1150
SCPGPDFTRW SQKSSAPRPC LPNLPAADDV VEVGDSDDEV ASHQGNSSPV 1200
LDGDPPGPMG DYCWNEPLSP IPIDHLNLER TGPLTTSSPS SQVLEALHSD 1250
DCHSPGLGTT PIRGSCGTLR ESQERSSLAG SPEALWDDWN EEEGQSPEAP 1300
PVAQMLSTRT RKPDRPETPK GANQKKNLPP KVPITPMPRY SIMETPVLKK 1350
ELDRFGVRAL PKRQMVLKLK EIFQYTHQTL ESDSEDEVQS PQIPAELPCR 1400
QASTTETCNP SRLPTGEPSH PDGDAQLPAS QESMATSVDG SDNSFSSKSS 1450
SAEFGAAFEY SDEDKDEEVG VTASQAAIQA ADTEEAVRRY IRSKPALHRQ 1500
VLRYQPVELA ELQAELKQNG IPVAMGKLSD ILDAQCITFT TAAARKEKLK 1550
HKRRQPSGRK KKDQK 1565
Length:1,565
Mass (Da):172,412
Last modified:July 5, 2004 - v1
Checksum:iAB4CB4EB57CA6394
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC065125 mRNA. Translation: AAH65125.1.
CCDSiCCDS37240.1.
RefSeqiNP_803423.2. NM_177472.5.
UniGeneiMm.29935.

Genome annotation databases

EnsembliENSMUST00000040790; ENSMUSP00000038871; ENSMUSG00000039738.
GeneIDi52864.
KEGGimmu:52864.
UCSCiuc007xzg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC065125 mRNA. Translation: AAH65125.1 .
CCDSi CCDS37240.1.
RefSeqi NP_803423.2. NM_177472.5.
UniGenei Mm.29935.

3D structure databases

ProteinModelPortali Q6P1D7.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q6P1D7.

Proteomic databases

PaxDbi Q6P1D7.
PRIDEi Q6P1D7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040790 ; ENSMUSP00000038871 ; ENSMUSG00000039738 .
GeneIDi 52864.
KEGGi mmu:52864.
UCSCi uc007xzg.1. mouse.

Organism-specific databases

CTDi 84464.
MGIi MGI:106299. Slx4.

Phylogenomic databases

eggNOGi NOG44550.
GeneTreei ENSGT00390000014091.
HOGENOMi HOG000095273.
InParanoidi Q6P1D7.
KOi K10484.
OMAi HKFVLYA.
OrthoDBi EOG7ZKSDH.
PhylomeDBi Q6P1D7.
TreeFami TF106446.

Miscellaneous databases

NextBioi 309663.
PROi Q6P1D7.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6P1D7.
Bgeei Q6P1D7.
Genevestigatori Q6P1D7.

Family and domain databases

Gene3Di 3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR018574. Structure-sp_endonuc_su_Slx4.
[Graphical view ]
Pfami PF00651. BTB. 1 hit.
PF09494. Slx4. 1 hit.
[Graphical view ]
SMARTi SM00225. BTB. 1 hit.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
PROSITEi PS50097. BTB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. Cited for: MUTAGENESIS OF PRO-202; GLU-602; GLN-1324 AND ARG-1545.

Entry informationi

Entry nameiSLX4_MOUSE
AccessioniPrimary (citable) accession number: Q6P1D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi