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Q6P1D7

- SLX4_MOUSE

UniProt

Q6P1D7 - SLX4_MOUSE

Protein

Structure-specific endonuclease subunit SLX4

Gene

Slx4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks By similarity.By similarity

    GO - Molecular functioni

    1. 3'-flap endonuclease activity Source: Ensembl
    2. 5'-flap endonuclease activity Source: Ensembl
    3. crossover junction endodeoxyribonuclease activity Source: Ensembl
    4. enzyme activator activity Source: Ensembl

    GO - Biological processi

    1. DNA double-strand break processing involved in repair via single-strand annealing Source: Ensembl
    2. DNA replication Source: InterPro
    3. double-strand break repair via homologous recombination Source: Ensembl
    4. nucleotide-excision repair Source: Ensembl

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Structure-specific endonuclease subunit SLX4
    Alternative name(s):
    BTB/POZ domain-containing protein 12
    Gene namesi
    Name:Slx4
    Synonyms:Btbd12
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:106299. Slx4.

    Subcellular locationi

    Nucleus By similarity
    Note: Localizes to sites of DNA dammage.By similarity

    GO - Cellular componenti

    1. ERCC4-ERCC1 complex Source: Ensembl
    2. Holliday junction resolvase complex Source: Ensembl
    3. nuclear chromatin Source: Ensembl
    4. nuclear chromosome, telomeric region Source: Ensembl
    5. Slx1-Slx4 complex Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi202 – 2021P → T: Does not modify the functional properties of the protein. 1 Publication
    Mutagenesisi602 – 6021E → K: Does not modify the functional properties of the protein. 1 Publication
    Mutagenesisi1324 – 13241Q → W: Does not modify the functional properties of the protein. 1 Publication
    Mutagenesisi1545 – 15451R → C: Does not modify the functional properties of the protein. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15651565Structure-specific endonuclease subunit SLX4PRO_0000383567Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei111 – 1111PhosphoserineBy similarity
    Modified residuei845 – 8451PhosphoserineBy similarity
    Modified residuei1254 – 12541PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ6P1D7.
    PRIDEiQ6P1D7.

    PTM databases

    PhosphoSiteiQ6P1D7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6P1D7.
    BgeeiQ6P1D7.
    GenevestigatoriQ6P1D7.

    Interactioni

    Subunit structurei

    Forms a heterodimer with SLX1A/GIYD1. Interacts with ERCC4; catalytic subunit of the ERCC4-ERCC1 endonuclease. Interacts with MUS81; catalytic subunit of the MUS81-EME1 endonuclease. Interacts with MSH2; component of the MSH2-MSH3 mismatch repair complex. Interacts with TERF2-TERF2IP. Interacts with PLK1 and SLX4IP By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P1D7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini506 – 57974BTBPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni499 – 15651067Interaction with PLK1 and TERF2-TERF2IPBy similarityAdd
    BLAST
    Regioni1120 – 1413294Interaction with MUS81By similarityAdd
    BLAST
    Regioni1406 – 1565160Interaction with SLX1By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SLX4 family.Curated
    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG44550.
    GeneTreeiENSGT00390000014091.
    HOGENOMiHOG000095273.
    InParanoidiQ6P1D7.
    KOiK10484.
    OMAiHKFVLYA.
    OrthoDBiEOG7ZKSDH.
    PhylomeDBiQ6P1D7.
    TreeFamiTF106446.

    Family and domain databases

    Gene3Di3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR018574. Structure-sp_endonuc_su_Slx4.
    [Graphical view]
    PfamiPF00651. BTB. 1 hit.
    PF09494. Slx4. 1 hit.
    [Graphical view]
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6P1D7-1 [UniParc]FASTAAdd to Basket

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    MVPESAPNGN SQPLPSCFTT TGVPSPSKPR VSELVLQRMK QFKRADPERL     50
    RHASEESPQK TALGDDVPRS PPEETVGENE YKLDATDSDA AMALALQQEF 100
    RREEASSHHD SLEEKGLFFC QMCQKNLSAM NVTRREQHVN RCLDEAEKAQ 150
    RPASPRIPDC PICGKPFLTT KSRISHLKQC AVRMEVGPQL LLQAVRLQTA 200
    QPEVDGSPQV PSFSNNVGGL KRKGVTTKRE PRRRKVNKPE APSEDLLVAM 250
    ALSRSEVEHC PVVPPLRLEN AFSEKIRLGA EKKSRKKRPP VCPPQLVTQD 300
    SETTGRQIED RVAQLLSEEA ELSCTPPLLA SKISKEELEP AGWRARLPEG 350
    KRNFLWELSA LTGAWAEESF YTVGLFPPIV SQCPSKEPQL PLELPKQGEP 400
    SPRRPPASQS SLPVSHSPKI RLLSSSQRER QALQDLVDLA VEGLSSSPQP 450
    GSRGVPTGLD LVPSSLPLTG FVLPCKKTLK KDDSASLSLG LLVTDFGAMV 500
    NNPHLSDVQF QLDSGEVLYA HKFVLYARCP LLIQYVSTES FSSEEDGDLT 550
    QRALLSDVSS EAAHAFLNYL YMADTDMPPS LVPDLRSLAL RFGVSDLVQL 600
    CEQVPAVVDL EGEQPEETSE DCESRAETFL ELLRSVWVDN EEEVETLLKP 650
    ELCEEERERV NEAEMEEIYE FAATQRKLLQ WGRAADPDGS TNPHGEDGAV 700
    SEPSLAGVQS NRQLENTEHM ESSGLEKEEA LASWEQEGHS TPLQDQCPDW 750
    AGKAEAQDAL GEATDDPSFC SRHRRGKECL PLHPNKAHGC KQPLPSNPRV 800
    SSELSQITVD HEEQSDHVRE TQADMAQAPT PHSCSLVSQS SVDGSPSQSW 850
    LHLYHTSHLS PSVSQSHSSI SRVASPRSLS PTTPTKQRRG SNIVTLRKDA 900
    GHHRGQQSSP IAGHRNRGIL ISPAKSPPID LTQSVPEPLS PRAQDPLHFV 950
    KKEDEVILLL DSDEELEHTK TESVSKDSPE GRKVPEFSPR SSELFSVIDV 1000
    EEDHEHFQSP LKREAGLQHG EEGQLGNQSA LGCRDIPWLL CSQKTSLDED 1050
    SATDTSWLVP ATPGVSRSRD CSSQTQIKSL KTRIPSDETA QQTPRPNLER 1100
    RTMLETAQQF SVIMPHTQPI TLGAFDSGRQ AYRSPSHPYP RHHRLSSSQP 1150
    SCPGPDFTRW SQKSSAPRPC LPNLPAADDV VEVGDSDDEV ASHQGNSSPV 1200
    LDGDPPGPMG DYCWNEPLSP IPIDHLNLER TGPLTTSSPS SQVLEALHSD 1250
    DCHSPGLGTT PIRGSCGTLR ESQERSSLAG SPEALWDDWN EEEGQSPEAP 1300
    PVAQMLSTRT RKPDRPETPK GANQKKNLPP KVPITPMPRY SIMETPVLKK 1350
    ELDRFGVRAL PKRQMVLKLK EIFQYTHQTL ESDSEDEVQS PQIPAELPCR 1400
    QASTTETCNP SRLPTGEPSH PDGDAQLPAS QESMATSVDG SDNSFSSKSS 1450
    SAEFGAAFEY SDEDKDEEVG VTASQAAIQA ADTEEAVRRY IRSKPALHRQ 1500
    VLRYQPVELA ELQAELKQNG IPVAMGKLSD ILDAQCITFT TAAARKEKLK 1550
    HKRRQPSGRK KKDQK 1565
    Length:1,565
    Mass (Da):172,412
    Last modified:July 5, 2004 - v1
    Checksum:iAB4CB4EB57CA6394
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC065125 mRNA. Translation: AAH65125.1.
    CCDSiCCDS37240.1.
    RefSeqiNP_803423.2. NM_177472.5.
    UniGeneiMm.29935.

    Genome annotation databases

    EnsembliENSMUST00000040790; ENSMUSP00000038871; ENSMUSG00000039738.
    GeneIDi52864.
    KEGGimmu:52864.
    UCSCiuc007xzg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC065125 mRNA. Translation: AAH65125.1 .
    CCDSi CCDS37240.1.
    RefSeqi NP_803423.2. NM_177472.5.
    UniGenei Mm.29935.

    3D structure databases

    ProteinModelPortali Q6P1D7.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6P1D7.

    Proteomic databases

    PaxDbi Q6P1D7.
    PRIDEi Q6P1D7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000040790 ; ENSMUSP00000038871 ; ENSMUSG00000039738 .
    GeneIDi 52864.
    KEGGi mmu:52864.
    UCSCi uc007xzg.1. mouse.

    Organism-specific databases

    CTDi 84464.
    MGIi MGI:106299. Slx4.

    Phylogenomic databases

    eggNOGi NOG44550.
    GeneTreei ENSGT00390000014091.
    HOGENOMi HOG000095273.
    InParanoidi Q6P1D7.
    KOi K10484.
    OMAi HKFVLYA.
    OrthoDBi EOG7ZKSDH.
    PhylomeDBi Q6P1D7.
    TreeFami TF106446.

    Miscellaneous databases

    NextBioi 309663.
    PROi Q6P1D7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6P1D7.
    Bgeei Q6P1D7.
    Genevestigatori Q6P1D7.

    Family and domain databases

    Gene3Di 3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR018574. Structure-sp_endonuc_su_Slx4.
    [Graphical view ]
    Pfami PF00651. BTB. 1 hit.
    PF09494. Slx4. 1 hit.
    [Graphical view ]
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. Cited for: MUTAGENESIS OF PRO-202; GLU-602; GLN-1324 AND ARG-1545.

    Entry informationi

    Entry nameiSLX4_MOUSE
    AccessioniPrimary (citable) accession number: Q6P1D7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3