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Protein

Structure-specific endonuclease subunit SLX4

Gene

Slx4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks (By similarity).By similarity

GO - Molecular functioni

  1. 3'-flap endonuclease activity Source: Ensembl
  2. 5'-flap endonuclease activity Source: Ensembl
  3. crossover junction endodeoxyribonuclease activity Source: Ensembl
  4. enzyme activator activity Source: MGI

GO - Biological processi

  1. DNA catabolic process, endonucleolytic Source: MGI
  2. DNA double-strand break processing involved in repair via single-strand annealing Source: MGI
  3. DNA repair Source: MGI
  4. DNA replication Source: InterPro
  5. double-strand break repair via homologous recombination Source: MGI
  6. nucleotide-excision repair Source: MGI
  7. positive regulation of catalytic activity Source: MGI
  8. response to intra-S DNA damage checkpoint signaling Source: MGI
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Structure-specific endonuclease subunit SLX4
Alternative name(s):
BTB/POZ domain-containing protein 12
Gene namesi
Name:Slx4
Synonyms:Btbd12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:106299. Slx4.

Subcellular locationi

Nucleus By similarity
Note: Localizes to sites of DNA dammage.By similarity

GO - Cellular componenti

  1. cell junction Source: MGI
  2. cytoplasm Source: MGI
  3. ERCC4-ERCC1 complex Source: Ensembl
  4. Holliday junction resolvase complex Source: Ensembl
  5. nuclear chromatin Source: Ensembl
  6. nuclear chromosome, telomeric region Source: Ensembl
  7. nucleoplasm Source: MGI
  8. Slx1-Slx4 complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi202 – 2021P → T: Does not modify the functional properties of the protein. 1 Publication
Mutagenesisi602 – 6021E → K: Does not modify the functional properties of the protein. 1 Publication
Mutagenesisi1324 – 13241Q → W: Does not modify the functional properties of the protein. 1 Publication
Mutagenesisi1545 – 15451R → C: Does not modify the functional properties of the protein. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15651565Structure-specific endonuclease subunit SLX4PRO_0000383567Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei111 – 1111PhosphoserineBy similarity
Modified residuei845 – 8451PhosphoserineBy similarity
Modified residuei1254 – 12541PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6P1D7.
PRIDEiQ6P1D7.

PTM databases

PhosphoSiteiQ6P1D7.

Expressioni

Gene expression databases

BgeeiQ6P1D7.
ExpressionAtlasiQ6P1D7. baseline and differential.
GenevestigatoriQ6P1D7.

Interactioni

Subunit structurei

Forms a heterodimer with SLX1A/GIYD1. Interacts with ERCC4; catalytic subunit of the ERCC4-ERCC1 endonuclease. Interacts with MUS81; catalytic subunit of the MUS81-EME1 endonuclease. Interacts with MSH2; component of the MSH2-MSH3 mismatch repair complex. Interacts with TERF2-TERF2IP. Interacts with PLK1 and SLX4IP (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6P1D7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini506 – 57974BTBPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni499 – 15651067Interaction with PLK1 and TERF2-TERF2IPBy similarityAdd
BLAST
Regioni1120 – 1413294Interaction with MUS81By similarityAdd
BLAST
Regioni1406 – 1565160Interaction with SLX1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the SLX4 family.Curated
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG44550.
GeneTreeiENSGT00390000014091.
HOGENOMiHOG000095273.
InParanoidiQ6P1D7.
KOiK10484.
OMAiHKFVLYA.
OrthoDBiEOG7ZKSDH.
PhylomeDBiQ6P1D7.
TreeFamiTF106446.

Family and domain databases

InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR018574. Structure-sp_endonuc_su_Slx4.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF09494. Slx4. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P1D7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPESAPNGN SQPLPSCFTT TGVPSPSKPR VSELVLQRMK QFKRADPERL
60 70 80 90 100
RHASEESPQK TALGDDVPRS PPEETVGENE YKLDATDSDA AMALALQQEF
110 120 130 140 150
RREEASSHHD SLEEKGLFFC QMCQKNLSAM NVTRREQHVN RCLDEAEKAQ
160 170 180 190 200
RPASPRIPDC PICGKPFLTT KSRISHLKQC AVRMEVGPQL LLQAVRLQTA
210 220 230 240 250
QPEVDGSPQV PSFSNNVGGL KRKGVTTKRE PRRRKVNKPE APSEDLLVAM
260 270 280 290 300
ALSRSEVEHC PVVPPLRLEN AFSEKIRLGA EKKSRKKRPP VCPPQLVTQD
310 320 330 340 350
SETTGRQIED RVAQLLSEEA ELSCTPPLLA SKISKEELEP AGWRARLPEG
360 370 380 390 400
KRNFLWELSA LTGAWAEESF YTVGLFPPIV SQCPSKEPQL PLELPKQGEP
410 420 430 440 450
SPRRPPASQS SLPVSHSPKI RLLSSSQRER QALQDLVDLA VEGLSSSPQP
460 470 480 490 500
GSRGVPTGLD LVPSSLPLTG FVLPCKKTLK KDDSASLSLG LLVTDFGAMV
510 520 530 540 550
NNPHLSDVQF QLDSGEVLYA HKFVLYARCP LLIQYVSTES FSSEEDGDLT
560 570 580 590 600
QRALLSDVSS EAAHAFLNYL YMADTDMPPS LVPDLRSLAL RFGVSDLVQL
610 620 630 640 650
CEQVPAVVDL EGEQPEETSE DCESRAETFL ELLRSVWVDN EEEVETLLKP
660 670 680 690 700
ELCEEERERV NEAEMEEIYE FAATQRKLLQ WGRAADPDGS TNPHGEDGAV
710 720 730 740 750
SEPSLAGVQS NRQLENTEHM ESSGLEKEEA LASWEQEGHS TPLQDQCPDW
760 770 780 790 800
AGKAEAQDAL GEATDDPSFC SRHRRGKECL PLHPNKAHGC KQPLPSNPRV
810 820 830 840 850
SSELSQITVD HEEQSDHVRE TQADMAQAPT PHSCSLVSQS SVDGSPSQSW
860 870 880 890 900
LHLYHTSHLS PSVSQSHSSI SRVASPRSLS PTTPTKQRRG SNIVTLRKDA
910 920 930 940 950
GHHRGQQSSP IAGHRNRGIL ISPAKSPPID LTQSVPEPLS PRAQDPLHFV
960 970 980 990 1000
KKEDEVILLL DSDEELEHTK TESVSKDSPE GRKVPEFSPR SSELFSVIDV
1010 1020 1030 1040 1050
EEDHEHFQSP LKREAGLQHG EEGQLGNQSA LGCRDIPWLL CSQKTSLDED
1060 1070 1080 1090 1100
SATDTSWLVP ATPGVSRSRD CSSQTQIKSL KTRIPSDETA QQTPRPNLER
1110 1120 1130 1140 1150
RTMLETAQQF SVIMPHTQPI TLGAFDSGRQ AYRSPSHPYP RHHRLSSSQP
1160 1170 1180 1190 1200
SCPGPDFTRW SQKSSAPRPC LPNLPAADDV VEVGDSDDEV ASHQGNSSPV
1210 1220 1230 1240 1250
LDGDPPGPMG DYCWNEPLSP IPIDHLNLER TGPLTTSSPS SQVLEALHSD
1260 1270 1280 1290 1300
DCHSPGLGTT PIRGSCGTLR ESQERSSLAG SPEALWDDWN EEEGQSPEAP
1310 1320 1330 1340 1350
PVAQMLSTRT RKPDRPETPK GANQKKNLPP KVPITPMPRY SIMETPVLKK
1360 1370 1380 1390 1400
ELDRFGVRAL PKRQMVLKLK EIFQYTHQTL ESDSEDEVQS PQIPAELPCR
1410 1420 1430 1440 1450
QASTTETCNP SRLPTGEPSH PDGDAQLPAS QESMATSVDG SDNSFSSKSS
1460 1470 1480 1490 1500
SAEFGAAFEY SDEDKDEEVG VTASQAAIQA ADTEEAVRRY IRSKPALHRQ
1510 1520 1530 1540 1550
VLRYQPVELA ELQAELKQNG IPVAMGKLSD ILDAQCITFT TAAARKEKLK
1560
HKRRQPSGRK KKDQK
Length:1,565
Mass (Da):172,412
Last modified:July 5, 2004 - v1
Checksum:iAB4CB4EB57CA6394
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC065125 mRNA. Translation: AAH65125.1.
CCDSiCCDS37240.1.
RefSeqiNP_803423.2. NM_177472.5.
UniGeneiMm.29935.

Genome annotation databases

EnsembliENSMUST00000040790; ENSMUSP00000038871; ENSMUSG00000039738.
GeneIDi52864.
KEGGimmu:52864.
UCSCiuc007xzg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC065125 mRNA. Translation: AAH65125.1.
CCDSiCCDS37240.1.
RefSeqiNP_803423.2. NM_177472.5.
UniGeneiMm.29935.

3D structure databases

ProteinModelPortaliQ6P1D7.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ6P1D7.

Proteomic databases

PaxDbiQ6P1D7.
PRIDEiQ6P1D7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040790; ENSMUSP00000038871; ENSMUSG00000039738.
GeneIDi52864.
KEGGimmu:52864.
UCSCiuc007xzg.1. mouse.

Organism-specific databases

CTDi84464.
MGIiMGI:106299. Slx4.

Phylogenomic databases

eggNOGiNOG44550.
GeneTreeiENSGT00390000014091.
HOGENOMiHOG000095273.
InParanoidiQ6P1D7.
KOiK10484.
OMAiHKFVLYA.
OrthoDBiEOG7ZKSDH.
PhylomeDBiQ6P1D7.
TreeFamiTF106446.

Miscellaneous databases

NextBioi309663.
PROiQ6P1D7.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P1D7.
ExpressionAtlasiQ6P1D7. baseline and differential.
GenevestigatoriQ6P1D7.

Family and domain databases

InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR018574. Structure-sp_endonuc_su_Slx4.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF09494. Slx4. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. Cited for: MUTAGENESIS OF PRO-202; GLU-602; GLN-1324 AND ARG-1545.

Entry informationi

Entry nameiSLX4_MOUSE
AccessioniPrimary (citable) accession number: Q6P1D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: March 4, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.