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Protein

Inositol-pentakisphosphate 2-kinase

Gene

Ippk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis.1 Publication

Catalytic activityi

ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol hexakisphosphate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1855167. Synthesis of pyrophosphates in the cytosol.
R-MMU-1855191. Synthesis of IPs in the nucleus.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-pentakisphosphate 2-kinase (EC:2.7.1.158)
Alternative name(s):
Inositol-1,3,4,5,6-pentakisphosphate 2-kinase
Ins(1,3,4,5,6)P5 2-kinase
Short name:
InsP5 2-kinase
Gene namesi
Name:Ippk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1922928. Ippk.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death during ermbryogenesis before 8.5 dpc, suggesting that InsP6 is required for yolk sac function and development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001105301 – 489Inositol-pentakisphosphate 2-kinaseAdd BLAST489

Proteomic databases

PaxDbiQ6P1C1.
PRIDEiQ6P1C1.

PTM databases

iPTMnetiQ6P1C1.
PhosphoSitePlusiQ6P1C1.

Expressioni

Tissue specificityi

In brain, it is expressed throughout the hippocampus (CA1, CA2, CA3 and dentate gyrus), inner layers of the cerebral cortex, and Purkinje cells of the cerebellum. In heart, it is expressed in cardiomyocytes but not in interstitial cells, blood vessels, or valves. Also expressed in testis.1 Publication

Developmental stagei

In E9 embryos it is expressed in regions corresponding to the neural tube, notochord and somites. Through the neural tube, it is expressed in the ventricular zone and in migrating neuroblasts. Also expressed in the notochord and specific regions of the somite. Strongly expressed in the myotome (future skeletal muscle) of the somite. In addition to the neural tube, it is also expressed in the ventricular zone and migrating neuroblasts throughout the embryonic brain. Prominent expression is detected in the yolk sac and embryonic heart. Within the yolk sac, it is expressed in both the epithelial and endothelial layers (blood vessels) but absent from the blood islands. In the heart, it is detected in both the atrial and ventricular chambers. In the ventricular myocardium it is present in both the endocardium and myocardium. Also expressed in the cardinal vein, aorta, digestive tract, and pharyngeal arches.

Gene expression databases

BgeeiENSMUSG00000021385.
CleanExiMM_IPPK.
GenevisibleiQ6P1C1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021817.

Structurei

Secondary structure

1489
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 10Combined sources3
Beta strandi11 – 16Combined sources6
Beta strandi18 – 37Combined sources20
Helixi45 – 61Combined sources17
Helixi63 – 67Combined sources5
Turni69 – 71Combined sources3
Beta strandi77 – 79Combined sources3
Helixi82 – 92Combined sources11
Turni93 – 95Combined sources3
Helixi98 – 102Combined sources5
Beta strandi103 – 105Combined sources3
Beta strandi111 – 116Combined sources6
Turni118 – 120Combined sources3
Turni123 – 125Combined sources3
Beta strandi133 – 137Combined sources5
Helixi153 – 156Combined sources4
Helixi160 – 170Combined sources11
Beta strandi173 – 176Combined sources4
Helixi182 – 185Combined sources4
Beta strandi186 – 188Combined sources3
Helixi190 – 202Combined sources13
Turni205 – 207Combined sources3
Beta strandi208 – 214Combined sources7
Helixi230 – 241Combined sources12
Helixi254 – 269Combined sources16
Beta strandi275 – 277Combined sources3
Beta strandi289 – 291Combined sources3
Beta strandi315 – 317Combined sources3
Helixi318 – 326Combined sources9
Helixi333 – 349Combined sources17
Helixi353 – 356Combined sources4
Turni357 – 360Combined sources4
Helixi365 – 370Combined sources6
Helixi382 – 399Combined sources18
Beta strandi402 – 409Combined sources8
Beta strandi425 – 427Combined sources3
Beta strandi429 – 436Combined sources8
Helixi443 – 445Combined sources3
Helixi446 – 463Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MW8X-ray2.40A1-468[»]
5MWLX-ray3.20A/B1-468[»]
5MWMX-ray2.60A1-468[»]
ProteinModelPortaliQ6P1C1.
SMRiQ6P1C1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi136 – 140EXKPK motif5

Domaini

The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.

Sequence similaritiesi

Belongs to the IPK1 type 2 family.Curated

Phylogenomic databases

eggNOGiKOG4749. Eukaryota.
ENOG410XSF2. LUCA.
GeneTreeiENSGT00390000010053.
HOGENOMiHOG000113092.
HOVERGENiHBG079461.
InParanoidiQ6P1C1.
KOiK10572.
OMAiMDEGKMD.
OrthoDBiEOG091G05DL.
PhylomeDBiQ6P1C1.
TreeFamiTF106142.

Family and domain databases

InterProiView protein in InterPro
IPR009286. Ins_P5_2-kin.
PANTHERiPTHR14456. PTHR14456. 1 hit.
PfamiView protein in Pfam
PF06090. Ins_P5_2-kin. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6P1C1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEGKMDENE WSYHGEGNKS LVVAHAQRCV VLRFLKFPPN KKKTSEEILQ
60 70 80 90 100
HLQNIVDFGK NVMKDFLGEN YVHCGEVVQL PLEFVKQLCL KIQCERPESR
110 120 130 140 150
CDKDLDTFSG YAMCLPNLTR LQTFHFAEHR PILCVEIKPK CGFIPFSNDV
160 170 180 190 200
THEMKHKVCR YCMHQHLKVA TGKWKKISKY CPLDLYSGNK QRMHFALRSL
210 220 230 240 250
LQETQNNLRI FKNGELIYGC GDARSPVADL KELAHHLKPF FFPSNGLASG
260 270 280 290 300
PHCTKAVIRE LVHVITRVLL SSSEKARAGA LRLGLQGPRV CEASPFSRSL
310 320 330 340 350
HNQGKNTSEH SGLPKGCLLY KTLQVQMLDQ LDIEGLYPLY KRVEQYLEEF
360 370 380 390 400
PEERKTLQID GPYDEVFYQK LLDLSTEDDG TVAFALTKVQ QYRVAMTAKD
410 420 430 440 450
CSIMIALSPC LQGTSSDQRP VIPSSRSRLA FSVSVLDLDL KPYESIPHQY
460 470 480
KLDSKIVNYY SKTVHAKDDT VRSTRFKEHE DCTLVLHKV
Length:489
Mass (Da):55,928
Last modified:July 5, 2004 - v1
Checksum:i441839EA3F425D5A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83E → Q in BAE20447 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029085 mRNA. Translation: BAE20447.1.
AK160092 mRNA. Translation: BAE35623.1.
AK167467 mRNA. Translation: BAE39551.1.
BC062167 mRNA. Translation: AAH62167.1.
BC065157 mRNA. Translation: AAH65157.1.
CCDSiCCDS26500.1.
RefSeqiNP_951011.1. NM_199056.3.
UniGeneiMm.390586.

Genome annotation databases

EnsembliENSMUST00000220447; ENSMUSP00000152331; ENSMUSG00000021385.
GeneIDi75678.
KEGGimmu:75678.
UCSCiuc007qjj.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiIPPK_MOUSE
AccessioniPrimary (citable) accession number: Q6P1C1
Secondary accession number(s): Q3V3Z0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 5, 2004
Last modified: August 30, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families