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Protein

Xaa-Pro aminopeptidase 1

Gene

Xpnpep1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro (By similarity).By similarity

Catalytic activityi

Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.By similarity

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi415 – 4151Manganese 1By similarity
Metal bindingi426 – 4261Manganese 1By similarity
Metal bindingi426 – 4261Manganese 2By similarity
Metal bindingi489 – 4891Manganese 2By similarity
Metal bindingi523 – 5231Manganese 1By similarity
Metal bindingi523 – 5231Manganese 2By similarity
Metal bindingi537 – 5371Manganese 1By similarity
Metal bindingi537 – 5371Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

MEROPSiM24.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Xaa-Pro aminopeptidase 1 (EC:3.4.11.9)
Alternative name(s):
Aminoacylproline aminopeptidase
Cytosolic aminopeptidase P
Soluble aminopeptidase P
Short name:
sAmp
X-Pro aminopeptidase 1
X-prolyl aminopeptidase 1, soluble
Gene namesi
Name:Xpnpep1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2180003. Xpnpep1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 623622Xaa-Pro aminopeptidase 1PRO_0000185084Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei304 – 3041N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ6P1B1.
MaxQBiQ6P1B1.
PaxDbiQ6P1B1.
PRIDEiQ6P1B1.

PTM databases

iPTMnetiQ6P1B1.
PhosphoSiteiQ6P1B1.

Expressioni

Gene expression databases

BgeeiQ6P1B1.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228414. 1 interaction.
IntActiQ6P1B1. 4 interactions.
MINTiMINT-4121768.
STRINGi10090.ENSMUSP00000070946.

Structurei

3D structure databases

ProteinModelPortaliQ6P1B1.
SMRiQ6P1B1. Positions 3-619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24B family.Sequence analysis

Phylogenomic databases

eggNOGiKOG2413. Eukaryota.
COG0006. LUCA.
HOGENOMiHOG000255713.
HOVERGENiHBG002934.
InParanoidiQ6P1B1.
KOiK01262.
OrthoDBiEOG7BGHK4.
PhylomeDBiQ6P1B1.
TreeFamiTF314183.

Family and domain databases

Gene3Di3.40.350.10. 2 hits.
3.90.230.10. 1 hit.
InterProiIPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR000994. Pept_M24_structural-domain.
IPR032416. Peptidase_M24_C.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
PF16188. Peptidase_M24_C. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6P1B1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKVTSELL RQLRQAMRNS EYVAEPIQAY IIPSGDAHQS EYIAPCDCRR
60 70 80 90 100
AFVSGFDGSA GTAIITEEHA AMWTDGRYFL QAAKQMDNNW TLMKMGLKDT
110 120 130 140 150
PTQEDWLVSV LPEGSRVGVD PLIIPTDYWK KMAKVLRSAG HHLVPVKENL
160 170 180 190 200
VDKIWTDRPE RPCKPLLTLG LDYTGISWKE KVADLRLKMA ERSIAWFVVT
210 220 230 240 250
ALDEIAWLFN LRGSDVEHNP VFFSYAIVGL ETIMLFIDGD RVDAPGVKQH
260 270 280 290 300
LLLDLGLEAE YRIQVLPYKS ILSELKALCA DLSPREKVWV SDKASYAVSE
310 320 330 340 350
AIPKDHRCCM PYTPICIAKA VKNSAESDGM RRAHIKDAVA LCELFNWLEQ
360 370 380 390 400
EVPKGGVTEI SAADKAEEFR RQQADFVDLS FPTISSTGPN GAIIHYAPVP
410 420 430 440 450
ETNRTLSLDE VYLIDSGAQY KDGTTDVTRT MHFGTPTAYE KECFTYVLKG
460 470 480 490 500
HIAVSAAVFP TGTKGHLLDS FARSALWDSG LDYLHGTGHG VGSFLNVHEG
510 520 530 540 550
PCGISYKTFS DEPLEAGMIV TDEPGYYEDG AFGIRIENVV LVVPAKTKYN
560 570 580 590 600
FNNRGSLTFE PLTLVPIQTK MIDVNALTDK ECDWLNSYHQ TCRDVVGKEL
610 620
QSQGRQEALE WLIRETEPVS RQH
Length:623
Mass (Da):69,591
Last modified:July 5, 2004 - v1
Checksum:i3DACC2C5EDA74B9A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti347 – 3471W → R in AAK48945 (Ref. 1) Curated
Sequence conflicti457 – 4571A → T in AAK48945 (Ref. 1) Curated
Sequence conflicti544 – 5441P → A in AAK48945 (Ref. 1) Curated
Sequence conflicti552 – 5521N → T in AAK48945 (Ref. 1) Curated
Sequence conflicti556 – 5561S → T in AAK48945 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF363970 mRNA. Translation: AAK48945.1.
BC065174 mRNA. Translation: AAH65174.1.
RefSeqiNP_573479.3. NM_133216.3.
XP_011245461.1. XM_011247159.1.
XP_011245462.1. XM_011247160.1.
UniGeneiMm.99776.

Genome annotation databases

GeneIDi170750.
KEGGimmu:170750.
UCSCiuc008hwf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF363970 mRNA. Translation: AAK48945.1.
BC065174 mRNA. Translation: AAH65174.1.
RefSeqiNP_573479.3. NM_133216.3.
XP_011245461.1. XM_011247159.1.
XP_011245462.1. XM_011247160.1.
UniGeneiMm.99776.

3D structure databases

ProteinModelPortaliQ6P1B1.
SMRiQ6P1B1. Positions 3-619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228414. 1 interaction.
IntActiQ6P1B1. 4 interactions.
MINTiMINT-4121768.
STRINGi10090.ENSMUSP00000070946.

Protein family/group databases

MEROPSiM24.009.

PTM databases

iPTMnetiQ6P1B1.
PhosphoSiteiQ6P1B1.

Proteomic databases

EPDiQ6P1B1.
MaxQBiQ6P1B1.
PaxDbiQ6P1B1.
PRIDEiQ6P1B1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi170750.
KEGGimmu:170750.
UCSCiuc008hwf.2. mouse.

Organism-specific databases

CTDi7511.
MGIiMGI:2180003. Xpnpep1.

Phylogenomic databases

eggNOGiKOG2413. Eukaryota.
COG0006. LUCA.
HOGENOMiHOG000255713.
HOVERGENiHBG002934.
InParanoidiQ6P1B1.
KOiK01262.
OrthoDBiEOG7BGHK4.
PhylomeDBiQ6P1B1.
TreeFamiTF314183.

Miscellaneous databases

PROiQ6P1B1.
SOURCEiSearch...

Gene expression databases

BgeeiQ6P1B1.

Family and domain databases

Gene3Di3.40.350.10. 2 hits.
3.90.230.10. 1 hit.
InterProiIPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000587. Creatinase_N.
IPR000994. Pept_M24_structural-domain.
IPR032416. Peptidase_M24_C.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF01321. Creatinase_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
PF16188. Peptidase_M24_C. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse soluble (cytosolic) aminopeptidase P."
    Ryan J.W., Bondarev I.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: LiverImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported.
    Tissue: BrainImported.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiXPP1_MOUSE
AccessioniPrimary (citable) accession number: Q6P1B1
Secondary accession number(s): Q91Y37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.