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Protein

Mis18-binding protein 1

Gene

MIS18BP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Mis18-binding protein 1
Alternative name(s):
Kinetochore-associated protein KNL-2 homolog
Short name:
HsKNL-2
P243
Gene namesi
Name:MIS18BP1
Synonyms:C14orf106, KIAA1903, KNL2, M18BP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20190. MIS18BP1.

Subcellular locationi

  • Nucleus
  • Chromosome
  • Chromosomecentromere

  • Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55320.
OpenTargetsiENSG00000129534.
PharmGKBiPA134927557.

Polymorphism and mutation databases

BioMutaiMIS18BP1.
DMDMi68052324.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001971401 – 1132Mis18-binding protein 1Add BLAST1132

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1
Modified residuei110PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei135PhosphoserineCombined sources1
Modified residuei172PhosphoserineCombined sources1
Modified residuei192PhosphoserineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Cross-linki639Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei653PhosphothreonineCombined sources1
Cross-linki742Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei772PhosphoserineCombined sources1
Modified residuei773PhosphoserineCombined sources1
Modified residuei821PhosphothreonineCombined sources1
Modified residuei824PhosphoserineCombined sources1
Modified residuei860PhosphoserineCombined sources1
Cross-linki899Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1008PhosphoserineCombined sources1
Modified residuei1086PhosphoserineCombined sources1
Modified residuei1087PhosphothreonineCombined sources1
Modified residuei1089PhosphothreonineCombined sources1
Modified residuei1104PhosphoserineCombined sources1
Modified residuei1116PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ6P0N0.
MaxQBiQ6P0N0.
PaxDbiQ6P0N0.
PeptideAtlasiQ6P0N0.
PRIDEiQ6P0N0.

PTM databases

iPTMnetiQ6P0N0.
PhosphoSitePlusiQ6P0N0.

Expressioni

Gene expression databases

BgeeiENSG00000129534.
CleanExiHS_C14orf106.
ExpressionAtlasiQ6P0N0. baseline and differential.
GenevisibleiQ6P0N0. HS.

Organism-specific databases

HPAiHPA006504.

Interactioni

Subunit structurei

Interacts (via N-terminus) with FLNA (via N-terminus) (By similarity). Interacts with SP1 (Probable). Interacts with MIS18A. Identified in a complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4.By similarityCurated2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
OIP5O434823EBI-9870821,EBI-536879

Protein-protein interaction databases

BioGridi120601. 18 interactors.
IntActiQ6P0N0. 8 interactors.
MINTiMINT-4992022.
STRINGi9606.ENSP00000309790.

Structurei

Secondary structure

11132
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi882 – 894Combined sources13
Beta strandi897 – 901Combined sources5
Helixi902 – 909Combined sources8
Turni910 – 912Combined sources3
Helixi915 – 924Combined sources10
Beta strandi925 – 929Combined sources5
Beta strandi934 – 936Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGXNMR-A877-936[»]
ProteinModelPortaliQ6P0N0.
SMRiQ6P0N0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6P0N0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini875 – 930SANTPROSITE-ProRule annotationAdd BLAST56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1010 – 1013Poly-Asp4

Sequence similaritiesi

Contains 1 SANT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410II9U. Eukaryota.
ENOG41120B5. LUCA.
GeneTreeiENSGT00390000007395.
HOGENOMiHOG000113431.
InParanoidiQ6P0N0.
OMAiNDCDKYV.
OrthoDBiEOG091G050V.
PhylomeDBiQ6P0N0.
TreeFamiTF106401.

Family and domain databases

InterProiIPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015216. SANTA.
[Graphical view]
PfamiPF00249. Myb_DNA-binding. 1 hit.
PF09133. SANTA. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51293. SANT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P0N0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIATPLKHSR IYLPPEASSQ RRNLPMDAIF FDSIPSGTLT PVKDLVKYQN
60 70 80 90 100
SSLKLNDHKK NQFLKMTTFN NKNIFQSTML TEATTSNSSL DISAIKPNKD
110 120 130 140 150
GLKNKANYES PGKIFLRMKE KVLRDKQEQP SRNSSLLEPQ KSGNNETFTP
160 170 180 190 200
NRVEKKKLQH TYLCEEKENN KSFQSDDSSL RASVQGVPLE SSNNDIFLPV
210 220 230 240 250
KQKIQCQQEK KAPLHNLTYE LPTLNQEQEN FLAVEARNKT LTRAQLAKQI
260 270 280 290 300
FHSKESIVAT TKSKKDTFVL ESVDSADEQF QNTNAETLST NCIPIKNGSL
310 320 330 340 350
LMVSDSERTT EGTSQQKVKE GNGKTVPGET GLPGSMKDTC KIVLATPRLH
360 370 380 390 400
ITIPRRSKRN ISKLSPPRIF QTVTNGLKKN QVVQLQEWMI KSINNNTAIC
410 420 430 440 450
VEGKLIDVTN IYWHSNVIIE RIEHNKLRTI SGNVYILKGM IDQISMKEAG
460 470 480 490 500
YPNYLIRKFM FGFPENWKEH IDNFLEQLRA GEKNREKTKQ KQKTGRSVRD
510 520 530 540 550
IRKSMKNDAR ENQTDTAQRA TTTYDFDCDN LELKSNKHSE SPGATELNMC
560 570 580 590 600
HSNCQNKPTL RFPDDQVNNT IQNGGGDDLS NQELIGKKEY KMSSKKLKIG
610 620 630 640 650
ERTNERIIKS QKQETTEELD VSIDILTSRE QFFSDEERKY MAINQKKAYI
660 670 680 690 700
LVTPLKSRKV IEQRCMRYNL SAGTIKAVTD FVIPECQKKS PISKSMGTLE
710 720 730 740 750
NTFEGHKSKN KEDCDERDLL TVNRKIKISN LEKEQMLTSD FKKNTRLLPK
760 770 780 790 800
LKKIENQVAM SFYKHQSSPD LSSEESETEK EIKRKAEVKK TKAGNTKEAV
810 820 830 840 850
VHLRKSTRNT SNIPVILEPE TEESENEFYI KQKKARPSVK ETLQKSGVRK
860 870 880 890 900
EFPITEAVGS DKTNRHPLEC LPGLIQDKEW NEKELQKLHC AFASLPKHKP
910 920 930 940 950
GFWSEVAAAV GSRSPEECQR KYMENPRGKG SQKHVTKKKP ANSKGQNGKR
960 970 980 990 1000
GDADQKQTIK ITAKVGTLKR KQQMREFLEQ LPKDDHDDFF STTPLQHQRI
1010 1020 1030 1040 1050
LLPSFQDSED DDDILPNMDK NPTTPSSVIF PLVKTPQCQH VSPGMLGSIN
1060 1070 1080 1090 1100
RNDCDKYVFR MQKYHKSNGG IVWGNIKKKL VETDFSTPTP RRKTPFNTDL
1110 1120 1130
GENSGIGKLF TNAVESLDEE EKDYYFSNSD SA
Length:1,132
Mass (Da):129,085
Last modified:July 5, 2004 - v1
Checksum:i92F2787CD4687059
GO
Isoform 2 (identifier: Q6P0N0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     391-1132: Missing.

Note: No experimental confirmation available.
Show »
Length:390
Mass (Da):43,994
Checksum:iB4B1B945CE91C8A8
GO

Sequence cautioni

The sequence AK002048 differs from that shown. Reason: Frameshift at position 157.Curated
The sequence BAB67796 differs from that shown. Reason: Frameshift at position 154.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti510R → Q in BAB67796 (PubMed:11572484).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050184164C → R.Corresponds to variant rs1269008dbSNPEnsembl.1
Natural variantiVAR_050185347P → R.Corresponds to variant rs34168608dbSNPEnsembl.1
Natural variantiVAR_050186583E → D.Corresponds to variant rs34101857dbSNPEnsembl.1
Natural variantiVAR_050187851E → Q.Corresponds to variant rs34402741dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014105391 – 1132Missing in isoform 2. 1 PublicationAdd BLAST742

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067490 mRNA. Translation: BAB67796.1. Frameshift.
AK002048 mRNA. No translation available.
AL121809 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW65778.1.
CH471078 Genomic DNA. Translation: EAW65779.1.
CH471078 Genomic DNA. Translation: EAW65780.1.
BC051885 mRNA. Translation: AAH51885.1.
BC065544 mRNA. Translation: AAH65544.1.
AJ242977 mRNA. Translation: CAB45134.1.
CCDSiCCDS9684.1. [Q6P0N0-1]
RefSeqiNP_060823.3. NM_018353.4. [Q6P0N0-1]
XP_005267890.1. XM_005267833.4. [Q6P0N0-1]
XP_016876914.1. XM_017021425.1. [Q6P0N0-1]
UniGeneiHs.732769.

Genome annotation databases

EnsembliENST00000310806; ENSP00000309790; ENSG00000129534. [Q6P0N0-1]
GeneIDi55320.
KEGGihsa:55320.
UCSCiuc001wwf.3. human. [Q6P0N0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB067490 mRNA. Translation: BAB67796.1. Frameshift.
AK002048 mRNA. No translation available.
AL121809 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW65778.1.
CH471078 Genomic DNA. Translation: EAW65779.1.
CH471078 Genomic DNA. Translation: EAW65780.1.
BC051885 mRNA. Translation: AAH51885.1.
BC065544 mRNA. Translation: AAH65544.1.
AJ242977 mRNA. Translation: CAB45134.1.
CCDSiCCDS9684.1. [Q6P0N0-1]
RefSeqiNP_060823.3. NM_018353.4. [Q6P0N0-1]
XP_005267890.1. XM_005267833.4. [Q6P0N0-1]
XP_016876914.1. XM_017021425.1. [Q6P0N0-1]
UniGeneiHs.732769.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGXNMR-A877-936[»]
ProteinModelPortaliQ6P0N0.
SMRiQ6P0N0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120601. 18 interactors.
IntActiQ6P0N0. 8 interactors.
MINTiMINT-4992022.
STRINGi9606.ENSP00000309790.

PTM databases

iPTMnetiQ6P0N0.
PhosphoSitePlusiQ6P0N0.

Polymorphism and mutation databases

BioMutaiMIS18BP1.
DMDMi68052324.

Proteomic databases

EPDiQ6P0N0.
MaxQBiQ6P0N0.
PaxDbiQ6P0N0.
PeptideAtlasiQ6P0N0.
PRIDEiQ6P0N0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310806; ENSP00000309790; ENSG00000129534. [Q6P0N0-1]
GeneIDi55320.
KEGGihsa:55320.
UCSCiuc001wwf.3. human. [Q6P0N0-1]

Organism-specific databases

CTDi55320.
DisGeNETi55320.
GeneCardsiMIS18BP1.
HGNCiHGNC:20190. MIS18BP1.
HPAiHPA006504.
neXtProtiNX_Q6P0N0.
OpenTargetsiENSG00000129534.
PharmGKBiPA134927557.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410II9U. Eukaryota.
ENOG41120B5. LUCA.
GeneTreeiENSGT00390000007395.
HOGENOMiHOG000113431.
InParanoidiQ6P0N0.
OMAiNDCDKYV.
OrthoDBiEOG091G050V.
PhylomeDBiQ6P0N0.
TreeFamiTF106401.

Enzyme and pathway databases

ReactomeiR-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Miscellaneous databases

ChiTaRSiMIS18BP1. human.
EvolutionaryTraceiQ6P0N0.
GeneWikiiC14orf106.
GenomeRNAii55320.
PROiQ6P0N0.

Gene expression databases

BgeeiENSG00000129534.
CleanExiHS_C14orf106.
ExpressionAtlasiQ6P0N0. baseline and differential.
GenevisibleiQ6P0N0. HS.

Family and domain databases

InterProiIPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015216. SANTA.
[Graphical view]
PfamiPF00249. Myb_DNA-binding. 1 hit.
PF09133. SANTA. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS51293. SANT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiM18BP_HUMAN
AccessioniPrimary (citable) accession number: Q6P0N0
Secondary accession number(s): D3DSA7
, Q86V14, Q96PY4, Q9NUR5, Q9Y4X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.