Q6P0N0 (M18BP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mis18-binding protein 1

Alternative name(s):
Kinetochore-associated protein KNL-2 homolog
Short name=HsKNL-2
P243

Gene names

Name:	MIS18BP1
Synonyms:	C14orf106, KIAA1903, KNL2, M18BP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1132 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. Ref.9 Ref.10
Subunit structure	Interacts (via N-terminus) with FLNA (via N-terminus) By similarity. Interacts with SP1 Probable. Interacts with MIS18A. Identified in a complex containing MIS18A, MIS18B, MIS18BP1, RBBP7 and RBBP4. Ref.6 Ref.9
Subcellular location	Nucleus. Chromosome. Chromosome › centromere. Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin. Ref.9 Ref.10
Sequence similarities	Contains 1 SANT domain.
Sequence caution	The sequence AK002048 differs from that shown. Reason: Frameshift at position 157. The sequence BAB67796.1 differs from that shown. Reason: Frameshift at position 154.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Centromere Chromosome Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CenH3-containing nucleosome assembly at centromere Traceable author statement. Source: Reactome cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, centromeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Traceable author statement. Source: Reactome
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6P0N0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6P0N0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     391-1132: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1132

1132

Mis18-binding protein 1

PRO_0000197140

Regions

Domain

875 – 930

56

SANT

Compositional bias

1010 – 1013

4

Poly-Asp

Amino acid modifications

Modified residue

110

1

Phosphoserine Ref.12

Modified residue

135

1

Phosphoserine Ref.11

Modified residue

261

1

Phosphothreonine Ref.7

Modified residue

263

1

Phosphoserine Ref.7

Modified residue

299

1

Phosphoserine Ref.11

Modified residue

365

1

Phosphoserine Ref.11

Modified residue

541

1

Phosphoserine Ref.7

Modified residue

653

1

Phosphothreonine Ref.12 Ref.13

Modified residue

821

1

Phosphothreonine Ref.12

Modified residue

824

1

Phosphoserine Ref.8 Ref.12 Ref.13

Modified residue

993

1

Phosphothreonine Ref.8

Modified residue

1004

1

Phosphoserine Ref.7 Ref.13

Modified residue

1008

1

Phosphoserine Ref.12 Ref.13

Modified residue

1086

1

Phosphoserine Ref.12

Modified residue

1087

1

Phosphothreonine Ref.12

Modified residue

1089

1

Phosphothreonine Ref.12

Modified residue

1104

1

Phosphoserine Ref.8 Ref.12 Ref.13

Natural variations

Alternative sequence

391 – 1132

742

Missing in isoform 2.

VSP_014105

Natural variant

164

1

C → R.
Corresponds to variant rs1269008 [ dbSNP | Ensembl ].

VAR_050184

Natural variant

347

1

P → R.
Corresponds to variant rs34168608 [ dbSNP | Ensembl ].

VAR_050185

Natural variant

583

1

E → D.
Corresponds to variant rs34101857 [ dbSNP | Ensembl ].

VAR_050186

Natural variant

851

1

E → Q.
Corresponds to variant rs34402741 [ dbSNP | Ensembl ].

VAR_050187

Experimental info

Sequence conflict

510

1

R → Q in BAB67796. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

1132

Helix Strand Turn

Details...

Helix

882 – 894

13

Beta strand

897 – 901

5

Helix

902 – 909

8

Turn

910 – 912

3

Helix

915 – 924

10

Beta strand

925 – 929

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 92F2787CD4687059	FASTA	1,132	129,085
	10 20 30 40 50 60 MIATPLKHSR IYLPPEASSQ RRNLPMDAIF FDSIPSGTLT PVKDLVKYQN SSLKLNDHKK 70 80 90 100 110 120 NQFLKMTTFN NKNIFQSTML TEATTSNSSL DISAIKPNKD GLKNKANYES PGKIFLRMKE 130 140 150 160 170 180 KVLRDKQEQP SRNSSLLEPQ KSGNNETFTP NRVEKKKLQH TYLCEEKENN KSFQSDDSSL 190 200 210 220 230 240 RASVQGVPLE SSNNDIFLPV KQKIQCQQEK KAPLHNLTYE LPTLNQEQEN FLAVEARNKT 250 260 270 280 290 300 LTRAQLAKQI FHSKESIVAT TKSKKDTFVL ESVDSADEQF QNTNAETLST NCIPIKNGSL 310 320 330 340 350 360 LMVSDSERTT EGTSQQKVKE GNGKTVPGET GLPGSMKDTC KIVLATPRLH ITIPRRSKRN 370 380 390 400 410 420 ISKLSPPRIF QTVTNGLKKN QVVQLQEWMI KSINNNTAIC VEGKLIDVTN IYWHSNVIIE 430 440 450 460 470 480 RIEHNKLRTI SGNVYILKGM IDQISMKEAG YPNYLIRKFM FGFPENWKEH IDNFLEQLRA 490 500 510 520 530 540 GEKNREKTKQ KQKTGRSVRD IRKSMKNDAR ENQTDTAQRA TTTYDFDCDN LELKSNKHSE 550 560 570 580 590 600 SPGATELNMC HSNCQNKPTL RFPDDQVNNT IQNGGGDDLS NQELIGKKEY KMSSKKLKIG 610 620 630 640 650 660 ERTNERIIKS QKQETTEELD VSIDILTSRE QFFSDEERKY MAINQKKAYI LVTPLKSRKV 670 680 690 700 710 720 IEQRCMRYNL SAGTIKAVTD FVIPECQKKS PISKSMGTLE NTFEGHKSKN KEDCDERDLL 730 740 750 760 770 780 TVNRKIKISN LEKEQMLTSD FKKNTRLLPK LKKIENQVAM SFYKHQSSPD LSSEESETEK 790 800 810 820 830 840 EIKRKAEVKK TKAGNTKEAV VHLRKSTRNT SNIPVILEPE TEESENEFYI KQKKARPSVK 850 860 870 880 890 900 ETLQKSGVRK EFPITEAVGS DKTNRHPLEC LPGLIQDKEW NEKELQKLHC AFASLPKHKP 910 920 930 940 950 960 GFWSEVAAAV GSRSPEECQR KYMENPRGKG SQKHVTKKKP ANSKGQNGKR GDADQKQTIK 970 980 990 1000 1010 1020 ITAKVGTLKR KQQMREFLEQ LPKDDHDDFF STTPLQHQRI LLPSFQDSED DDDILPNMDK 1030 1040 1050 1060 1070 1080 NPTTPSSVIF PLVKTPQCQH VSPGMLGSIN RNDCDKYVFR MQKYHKSNGG IVWGNIKKKL 1090 1100 1110 1120 1130 VETDFSTPTP RRKTPFNTDL GENSGIGKLF TNAVESLDEE EKDYYFSNSD SA
	Isoform 2 [UniParc]. Checksum: B4B1B945CE91C8A8	FASTA	390	43,994
	10 20 30 40 50 60 MIATPLKHSR IYLPPEASSQ RRNLPMDAIF FDSIPSGTLT PVKDLVKYQN SSLKLNDHKK 70 80 90 100 110 120 NQFLKMTTFN NKNIFQSTML TEATTSNSSL DISAIKPNKD GLKNKANYES PGKIFLRMKE 130 140 150 160 170 180 KVLRDKQEQP SRNSSLLEPQ KSGNNETFTP NRVEKKKLQH TYLCEEKENN KSFQSDDSSL 190 200 210 220 230 240 RASVQGVPLE SSNNDIFLPV KQKIQCQQEK KAPLHNLTYE LPTLNQEQEN FLAVEARNKT 250 260 270 280 290 300 LTRAQLAKQI FHSKESIVAT TKSKKDTFVL ESVDSADEQF QNTNAETLST NCIPIKNGSL 310 320 330 340 350 360 LMVSDSERTT EGTSQQKVKE GNGKTVPGET GLPGSMKDTC KIVLATPRLH ITIPRRSKRN 370 380 390 ISKLSPPRIF QTVTNGLKKN QVVQLQEWMI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins." Nagase T., Kikuno R., Ohara O. DNA Res. 8:179-187(2001) [PubMed: 11572484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[3]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis and Uterus.
[6]	"A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening." Gunther M., Laithier M., Brison O. Mol. Cell. Biochem. 210:131-142(2000) [PubMed: 10976766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 523-760, INTERACTION WITH SP1. Tissue: Colon carcinoma.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-261; SER-263; SER-541 AND SER-1004, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-824; THR-993 AND SER-1104, MASS SPECTROMETRY. Tissue: Cervix adenocarcinoma.
[9]	"Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1." Fujita Y., Hayashi T., Kiyomitsu T., Toyoda Y., Kokubu A., Obuse C., Yanagida M. Dev. Cell 12:17-30(2007) [PubMed: 17199038] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, INTERACTION WITH MIS18A, IDENTIFICATION IN A COMPLEX CONTAINING MIS18A; MIS18B; MIS18BP1; RBBP7 AND RBBP4.
[10]	"Functional genomics identifies a Myb domain-containing protein family required for assembly of CENP-A chromatin." Maddox P.S., Hyndman F., Monen J., Oegema K., Desai A. J. Cell Biol. 176:757-763(2007) [PubMed: 17339379] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135; SER-299 AND SER-365, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; THR-653; THR-821; SER-824; SER-1008; SER-1086; THR-1087; THR-1089 AND SER-1104, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-653; SER-824; SER-1004; SER-1008 AND SER-1104, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[14]	"Solution structure of RSGI RUH-022, a Myb DNA-binding domain in human cDNA." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 877-936.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB067490 mRNA. Translation: BAB67796.1. Frameshift.
AK002048 mRNA. No translation available.
AL121809 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW65778.1.
CH471078 Genomic DNA. Translation: EAW65779.1.
CH471078 Genomic DNA. Translation: EAW65780.1.
BC051885 mRNA. Translation: AAH51885.1.
BC065544 mRNA. Translation: AAH65544.1.
AJ242977 mRNA. Translation: CAB45134.1.

IPI

IPI00104907.
IPI00385830.

RefSeq

NP_060823.3. NM_018353.4.

UniGene

Hs.437941.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WGX	NMR	-	A	877-936	[»]

ProteinModelPortal

Q6P0N0.

SMR

Q6P0N0. Positions 877-936.

ModBase

Search...

Protein-protein interaction databases

STRING

Q6P0N0.

PTM databases

PhosphoSite

Q6P0N0.

Polymorphism databases

DMDM

68052324.

Proteomic databases

PRIDE

Q6P0N0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000310806; ENSP00000309790; ENSG00000129534.

GeneID

55320.

KEGG

hsa:55320.

UCSC

uc001wwf.1. human.

Organism-specific databases

CTD

55320.

GeneCards

GC14M045673.

HGNC

HGNC:20190. MIS18BP1.

HPA

HPA006504.

neXtProt

NX_Q6P0N0.

PharmGKB

PA134927557.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG06570.

GeneTree

ENSGT00390000007395.

InParanoid

Q6P0N0.

OMA

NDCDKYV.

OrthoDB

EOG44QT27.

PhylomeDB

Q6P0N0.

Enzyme and pathway databases

Reactome

REACT_22172. Chromosome Maintenance.

Gene expression databases

ArrayExpress

Q6P0N0.

Bgee

Q6P0N0.

CleanEx

HS_C14orf106.

Genevestigator

Q6P0N0.

GermOnline

ENSG00000129534. Homo sapiens.

Family and domain databases

InterPro

IPR009057. Homeodomain-like.
IPR001005. SANT_DNA-bd.
IPR017884. SANT_eukarya.
IPR015216. SANTA.
[Graphical view]

Pfam

PF09133. SANTA. 1 hit.
[Graphical view]

SMART

SM00717. SANT. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.

PROSITE

PS51293. SANT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

59574.

Entry information

Entry name

M18BP_HUMAN

Accession

Primary (citable) accession number: Q6P0N0
Secondary accession number(s): D3DSA7 , Q86V14, Q96PY4, Q9NUR5, Q9Y4X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.