Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6P0N0 (M18BP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mis18-binding protein 1
Alternative name(s):
Kinetochore-associated protein KNL-2 homolog
Short name=HsKNL-2
P243
Gene names
Name:MIS18BP1
Synonyms:C14orf106, KIAA1903, KNL2, M18BP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1132 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. Ref.7 Ref.8

Subunit structure

Interacts (via N-terminus) with FLNA (via N-terminus) By similarity. Interacts with SP1 Probable. Interacts with MIS18A. Identified in a complex containing MIS18A, MIS18B, MIS18BP1, RBBP7 and RBBP4. Ref.6 Ref.7

Subcellular location

Nucleus. Chromosome. Chromosomecentromere. Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin. Ref.7 Ref.8

Sequence similarities

Contains 1 SANT domain.

Sequence caution

The sequence AK002048 differs from that shown. Reason: Frameshift at position 157.

The sequence BAB67796.1 differs from that shown. Reason: Frameshift at position 154.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6P0N0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6P0N0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     391-1132: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11321132Mis18-binding protein 1
PRO_0000197140

Regions

Domain875 – 93056SANT
Compositional bias1010 – 10134Poly-Asp

Amino acid modifications

Modified residue1101Phosphoserine Ref.9
Modified residue1351Phosphoserine Ref.13
Modified residue1721Phosphoserine Ref.13
Modified residue2991Phosphoserine Ref.13
Modified residue6531Phosphothreonine Ref.9 Ref.12 Ref.13
Modified residue7721Phosphoserine Ref.13
Modified residue7731Phosphoserine Ref.13
Modified residue8211Phosphothreonine Ref.9
Modified residue8241Phosphoserine Ref.9
Modified residue8601Phosphoserine Ref.12
Modified residue10081Phosphoserine Ref.9 Ref.11 Ref.12 Ref.13
Modified residue10861Phosphoserine Ref.9
Modified residue10871Phosphothreonine Ref.9
Modified residue10891Phosphothreonine Ref.9
Modified residue11041Phosphoserine Ref.9 Ref.11 Ref.12 Ref.13
Modified residue11161Phosphoserine Ref.12 Ref.13

Natural variations

Alternative sequence391 – 1132742Missing in isoform 2.
VSP_014105
Natural variant1641C → R.
Corresponds to variant rs1269008 [ dbSNP | Ensembl ].
VAR_050184
Natural variant3471P → R.
Corresponds to variant rs34168608 [ dbSNP | Ensembl ].
VAR_050185
Natural variant5831E → D.
Corresponds to variant rs34101857 [ dbSNP | Ensembl ].
VAR_050186
Natural variant8511E → Q.
Corresponds to variant rs34402741 [ dbSNP | Ensembl ].
VAR_050187

Experimental info

Sequence conflict5101R → Q in BAB67796. Ref.1

Secondary structure

............ 1132
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 92F2787CD4687059

FASTA1,132129,085
        10         20         30         40         50         60 
MIATPLKHSR IYLPPEASSQ RRNLPMDAIF FDSIPSGTLT PVKDLVKYQN SSLKLNDHKK 

        70         80         90        100        110        120 
NQFLKMTTFN NKNIFQSTML TEATTSNSSL DISAIKPNKD GLKNKANYES PGKIFLRMKE 

       130        140        150        160        170        180 
KVLRDKQEQP SRNSSLLEPQ KSGNNETFTP NRVEKKKLQH TYLCEEKENN KSFQSDDSSL 

       190        200        210        220        230        240 
RASVQGVPLE SSNNDIFLPV KQKIQCQQEK KAPLHNLTYE LPTLNQEQEN FLAVEARNKT 

       250        260        270        280        290        300 
LTRAQLAKQI FHSKESIVAT TKSKKDTFVL ESVDSADEQF QNTNAETLST NCIPIKNGSL 

       310        320        330        340        350        360 
LMVSDSERTT EGTSQQKVKE GNGKTVPGET GLPGSMKDTC KIVLATPRLH ITIPRRSKRN 

       370        380        390        400        410        420 
ISKLSPPRIF QTVTNGLKKN QVVQLQEWMI KSINNNTAIC VEGKLIDVTN IYWHSNVIIE 

       430        440        450        460        470        480 
RIEHNKLRTI SGNVYILKGM IDQISMKEAG YPNYLIRKFM FGFPENWKEH IDNFLEQLRA 

       490        500        510        520        530        540 
GEKNREKTKQ KQKTGRSVRD IRKSMKNDAR ENQTDTAQRA TTTYDFDCDN LELKSNKHSE 

       550        560        570        580        590        600 
SPGATELNMC HSNCQNKPTL RFPDDQVNNT IQNGGGDDLS NQELIGKKEY KMSSKKLKIG 

       610        620        630        640        650        660 
ERTNERIIKS QKQETTEELD VSIDILTSRE QFFSDEERKY MAINQKKAYI LVTPLKSRKV 

       670        680        690        700        710        720 
IEQRCMRYNL SAGTIKAVTD FVIPECQKKS PISKSMGTLE NTFEGHKSKN KEDCDERDLL 

       730        740        750        760        770        780 
TVNRKIKISN LEKEQMLTSD FKKNTRLLPK LKKIENQVAM SFYKHQSSPD LSSEESETEK 

       790        800        810        820        830        840 
EIKRKAEVKK TKAGNTKEAV VHLRKSTRNT SNIPVILEPE TEESENEFYI KQKKARPSVK 

       850        860        870        880        890        900 
ETLQKSGVRK EFPITEAVGS DKTNRHPLEC LPGLIQDKEW NEKELQKLHC AFASLPKHKP 

       910        920        930        940        950        960 
GFWSEVAAAV GSRSPEECQR KYMENPRGKG SQKHVTKKKP ANSKGQNGKR GDADQKQTIK 

       970        980        990       1000       1010       1020 
ITAKVGTLKR KQQMREFLEQ LPKDDHDDFF STTPLQHQRI LLPSFQDSED DDDILPNMDK 

      1030       1040       1050       1060       1070       1080 
NPTTPSSVIF PLVKTPQCQH VSPGMLGSIN RNDCDKYVFR MQKYHKSNGG IVWGNIKKKL 

      1090       1100       1110       1120       1130 
VETDFSTPTP RRKTPFNTDL GENSGIGKLF TNAVESLDEE EKDYYFSNSD SA 

« Hide

Isoform 2 [UniParc].

Checksum: B4B1B945CE91C8A8
Show »

FASTA39043,994

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins."
Nagase T., Kikuno R., Ohara O.
DNA Res. 8:179-187(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Placenta.
[3]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis and Uterus.
[6]"A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening."
Gunther M., Laithier M., Brison O.
Mol. Cell. Biochem. 210:131-142(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 523-760, INTERACTION WITH SP1.
Tissue: Colon carcinoma.
[7]"Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1."
Fujita Y., Hayashi T., Kiyomitsu T., Toyoda Y., Kokubu A., Obuse C., Yanagida M.
Dev. Cell 12:17-30(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MIS18A, IDENTIFICATION IN A COMPLEX CONTAINING MIS18A; MIS18B; MIS18BP1; RBBP7 AND RBBP4.
[8]"Functional genomics identifies a Myb domain-containing protein family required for assembly of CENP-A chromatin."
Maddox P.S., Hyndman F., Monen J., Oegema K., Desai A.
J. Cell Biol. 176:757-763(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; THR-653; THR-821; SER-824; SER-1008; SER-1086; THR-1087; THR-1089 AND SER-1104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1008 AND SER-1104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-653; SER-860; SER-1008; SER-1104 AND SER-1116, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135; SER-172; SER-299; THR-653; SER-772; SER-773; SER-1008; SER-1104 AND SER-1116, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Solution structure of RSGI RUH-022, a Myb DNA-binding domain in human cDNA."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 877-936.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB067490 mRNA. Translation: BAB67796.1. Frameshift.
AK002048 mRNA. No translation available.
AL121809 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW65778.1.
CH471078 Genomic DNA. Translation: EAW65779.1.
CH471078 Genomic DNA. Translation: EAW65780.1.
BC051885 mRNA. Translation: AAH51885.1.
BC065544 mRNA. Translation: AAH65544.1.
AJ242977 mRNA. Translation: CAB45134.1.
CCDSCCDS9684.1. [Q6P0N0-1]
RefSeqNP_060823.3. NM_018353.4. [Q6P0N0-1]
XP_005267890.1. XM_005267833.2. [Q6P0N0-1]
UniGeneHs.732769.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WGXNMR-A877-936[»]
ProteinModelPortalQ6P0N0.
SMRQ6P0N0. Positions 877-936.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120601. 4 interactions.
MINTMINT-4992022.
STRING9606.ENSP00000309790.

PTM databases

PhosphoSiteQ6P0N0.

Polymorphism databases

DMDM68052324.

Proteomic databases

MaxQBQ6P0N0.
PaxDbQ6P0N0.
PRIDEQ6P0N0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000310806; ENSP00000309790; ENSG00000129534. [Q6P0N0-1]
GeneID55320.
KEGGhsa:55320.
UCSCuc001wwf.3. human. [Q6P0N0-1]

Organism-specific databases

CTD55320.
GeneCardsGC14M045673.
HGNCHGNC:20190. MIS18BP1.
HPAHPA006504.
neXtProtNX_Q6P0N0.
PharmGKBPA134927557.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG73415.
HOGENOMHOG000113431.
InParanoidQ6P0N0.
OMANDCDKYV.
OrthoDBEOG77M8R3.
PhylomeDBQ6P0N0.
TreeFamTF106401.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.

Gene expression databases

ArrayExpressQ6P0N0.
BgeeQ6P0N0.
CleanExHS_C14orf106.
GenevestigatorQ6P0N0.

Family and domain databases

InterProIPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015216. SANTA.
[Graphical view]
PfamPF09133. SANTA. 1 hit.
[Graphical view]
SMARTSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS51293. SANT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ6P0N0.
GeneWikiC14orf106.
GenomeRNAi55320.
NextBio59574.
PROQ6P0N0.

Entry information

Entry nameM18BP_HUMAN
AccessionPrimary (citable) accession number: Q6P0N0
Secondary accession number(s): D3DSA7 expand/collapse secondary AC list , Q86V14, Q96PY4, Q9NUR5, Q9Y4X9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM