Q6P0K8 (PLAK_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Junction plakoglobin

Gene names

Name:

Jup

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	745 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton By similarity.
Subunit structure	Homodimer. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma-catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1. Interacts with PTPRJ. Interacts with DSG1. Interacts with DSC1 and DSC2 By similarity.
Subcellular location	Cell junction › adherens junction By similarity. Cell junction › desmosome By similarity. Cytoplasm › cytoskeleton By similarity. Membrane; Peripheral membrane protein By similarity. Note: Cytoplasmic in a soluble and membrane-associated form By similarity.
Domain	the whole ARM repeats region mediate binding to CDH1/E-cadherin By similarity.
Post-translational modification	May be phosphorylated by FER By similarity.
Sequence similarities	Belongs to the beta-catenin family. Contains 12 ARM repeats.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell junction Cytoplasm Cytoskeleton Membrane
Domain	Repeat
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	atrioventricular valve morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome bundle of His cell to Purkinje myocyte communication Inferred from electronic annotation. Source: Compara cell migration Inferred from electronic annotation. Source: Compara cell morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome cell-cell adhesion Inferred from direct assay PubMed 9023350. Source: RGD cellular response to indole-3-methanol Inferred from electronic annotation. Source: Compara cytoskeletal anchoring at plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome desmosome assembly Inferred from electronic annotation. Source: Compara detection of mechanical stimulus Inferred from electronic annotation. Source: Compara ectoderm development Inferred from Biological aspect of Ancestor. Source: RefGenome gastrulation Inferred from Biological aspect of Ancestor. Source: RefGenome morphogenesis of embryonic epithelium Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of Wnt receptor signaling pathway involved in heart development Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of heart induction by canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome nervous system development Inferred from Biological aspect of Ancestor. Source: RefGenome oocyte development Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of protein import into nucleus Inferred from electronic annotation. Source: Compara positive regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara protein heterooligomerization Inferred from physical interaction PubMed 8834786. Source: RGD regulation of apoptotic process Inferred from electronic annotation. Source: Compara regulation of cell proliferation Inferred from electronic annotation. Source: Compara regulation of heart rate by cardiac conduction Inferred from electronic annotation. Source: Compara regulation of ventricular cardiac muscle cell action potential Inferred from electronic annotation. Source: Compara skin development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	Z disc Inferred from Biological aspect of Ancestor. Source: RefGenome apicolateral plasma membrane Inferred from direct assay PubMed 10335358. Source: RGD basolateral plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome catenin complex Inferred from Biological aspect of Ancestor. Source: RefGenome cytosol Inferred from Biological aspect of Ancestor. Source: RefGenome desmosome Inferred from direct assay PubMed 16406610. Source: RGD fascia adherens Inferred from direct assay PubMed 16406610. Source: RGD gamma-catenin-TCF7L2 complex Inferred from electronic annotation. Source: Compara intermediate filament Inferred from electronic annotation. Source: Compara internal side of plasma membrane Inferred from direct assay PubMed 12478657. Source: RGD lateral plasma membrane Inferred from direct assay PubMed 10335358. Source: RGD protein-DNA complex Inferred from electronic annotation. Source: Compara zonula adherens Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	alpha-catenin binding Inferred from Biological aspect of Ancestor. Source: RefGenome cadherin binding Inferred from Biological aspect of Ancestor. Source: RefGenome structural molecule activity Inferred from direct assay PubMed 9023350. Source: RGD transcription coactivator activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 745	745	Junction plakoglobin		PRO_0000064281
Regions
	Repeat	132 – 171	40	ARM 1
	Repeat	172 – 215	44	ARM 2
	Repeat	216 – 255	40	ARM 3
	Repeat	258 – 297	40	ARM 4
	Repeat	298 – 341	44	ARM 5
	Repeat	342 – 381	40	ARM 6
	Repeat	383 – 420	38	ARM 7
	Repeat	423 – 464	42	ARM 8
	Repeat	470 – 510	41	ARM 9
	Repeat	512 – 551	40	ARM 10
	Repeat	574 – 613	40	ARM 11
	Repeat	615 – 661	47	ARM 12
	Region	132 – 297	166	Interaction with DSC1 and DSG1 By similarity
	Region	574 – 661	88	Interaction with DSC1 By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	182	1	Phosphoserine By similarity
	Modified residue	665	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6P0K8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BF8673E1B797954D	FASTA	745	81,801
	10 20 30 40 50 60 MEVMNLIEQP IKVTEWQQTY TYDSGIHSGV NTCVPSVSSK GLLDEDDTCG RQYTLKKTTT 70 80 90 100 110 120 YTQGVPQSQG DLEYQMSTTA RAKRVREAMC PGVSGEDSSL LLATQVEGQT TNLQRLAEPS 130 140 150 160 170 180 QLLKSAIVHL INYQDDAELA TRALPELTKL LNDEDPVVVT KAAMIVNQLS KKEASRRALM 190 200 210 220 230 240 GSPQLVAAVV RTMQNTSDLD TARCTTSILH NLSHHREGLL AIFKSGGIPA LVRMLSSPVE 250 260 270 280 290 300 SVLFYAITTL HNLLLYQEGA KMAVRLADGL QKMVPLLNKN NPKFLAITTD CLQLLAYGNQ 310 320 330 340 350 360 ESKLIILANG GPQGLVQIMR NYSYEKLLWT TSRVLKVLSV CPSNKPAIVE AGGMQALGKH 370 380 390 400 410 420 LTSNSPRLVQ NCLWTLRNLS DVATKQEGLE NVLKILVNQL SVDDVNVLTC ATGTLSNLTC 430 440 450 460 470 480 NNSKNKTLVT QNSGVEALIH AILRAGDKDD ITEPAVCALR HLTSRHPEAE MAQNSVRLNY 490 500 510 520 530 540 GIPAIVKLLN QPNQWPLVKA TIGLIRNLAL CPANHAPLQE AAVIPRLVQL LVKAHQDAQR 550 560 570 580 590 600 HVAAGTQQPY TDGVRMEEIV EGCTGALHIL ARDPMNRMEI FRLNTIPLFV QLLYSSVENI 610 620 630 640 650 660 QRVAAGVLCE LAQDKEAADA IDAEGASAPL MELLHSRNEG TATYAAAVLF RISEDKNPDY 670 680 690 700 710 720 RKRVSVELTN SLFKHDPAAW EAAQSMIPIN EPYADDMDAT YRPMYSSDVP LDPLDMHMDM 730 740 DGDYPMDTYS DGLRPPYPAA DHMLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[2]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-12; 58-81; 192-203 AND 262-279, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC065580 mRNA. Translation: AAH65580.1.
IPI	IPI00421429.
RefSeq	NP_112309.2. NM_031047.2.
UniGene	Rn.11255.
3D structure databases
ProteinModelPortal	Q6P0K8.
SMR	Q6P0K8. Positions 118-672.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6P0K8. 1 interaction.
PTM databases
PhosphoSite	Q6P0K8.
Proteomic databases
PaxDb	Q6P0K8.
PRIDE	Q6P0K8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000040845; ENSRNOP00000043950; ENSRNOG00000015380.
GeneID	81679.
KEGG	rno:81679.
UCSC	RGD:620412. rat.
Organism-specific databases
CTD	3728.
RGD	620412. Jup.
Phylogenomic databases
eggNOG	NOG297695.
GeneTree	ENSGT00680000099702.
HOGENOM	HOG000230958.
HOVERGEN	HBG000919.
InParanoid	Q6P0K8.
KO	K10056.
OMA	MNLIEQP.
OrthoDB	EOG4FR0R3.
Gene expression databases
Genevestigator	Q6P0K8.
GermOnline	ENSRNOG00000015380. Rattus norvegicus.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. IPR013284. Beta-catenin. [Graphical view]
Pfam	PF00514. Arm. 4 hits. [Graphical view]
PRINTS	PR01869. BCATNINFAMLY.
SMART	SM00185. ARM. 12 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50176. ARM_REPEAT. 9 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	615292.

Entry information

Entry name

PLAK_RAT

Accession

Primary (citable) accession number: Q6P0K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program