ID   ADCL2_HUMAN             Reviewed;         401 AA.
AC   Q6P093; Q5HYJ4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 3.
DT   24-JAN-2024, entry version 135.
DE   RecName: Full=Arylacetamide deacetylase-like 2;
DE            EC=3.1.1.-;
DE   Flags: Precursor;
GN   Name=AADACL2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Adipose tissue;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16641997; DOI=10.1038/nature04728;
RA   Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA   Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA   Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA   Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA   Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA   Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA   Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA   Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA   Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA   Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA   Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA   Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA   Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA   Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA   Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA   Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA   Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA   Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA   Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT   "The DNA sequence, annotation and analysis of human chromosome 3.";
RL   Nature 440:1194-1198(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT SER-186.
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6P093-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6P093-3; Sequence=VSP_038389, VSP_038390;
CC   -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH65724.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=CAI46075.1; Type=Miscellaneous discrepancy; Note=Wrong choice of CDS.; Evidence={ECO:0000305};
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DR   EMBL; BX647585; CAI46075.1; ALT_SEQ; mRNA.
DR   EMBL; AC069067; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC065724; AAH65724.1; ALT_INIT; mRNA.
DR   CCDS; CCDS3161.2; -. [Q6P093-1]
DR   RefSeq; NP_997248.2; NM_207365.3. [Q6P093-1]
DR   AlphaFoldDB; Q6P093; -.
DR   SMR; Q6P093; -.
DR   BioGRID; 131319; 1.
DR   STRING; 9606.ENSP00000348911; -.
DR   DrugBank; DB07814; Gibberellic acid.
DR   DrugBank; DB07815; Gibberellin A4.
DR   ESTHER; human-AADACL2; Arylacetamide_deacetylase.
DR   iPTMnet; Q6P093; -.
DR   PhosphoSitePlus; Q6P093; -.
DR   BioMuta; AADACL2; -.
DR   DMDM; 269849709; -.
DR   jPOST; Q6P093; -.
DR   MassIVE; Q6P093; -.
DR   PaxDb; 9606-ENSP00000348911; -.
DR   PeptideAtlas; Q6P093; -.
DR   ProteomicsDB; 66809; -. [Q6P093-1]
DR   Antibodypedia; 33616; 134 antibodies from 15 providers.
DR   DNASU; 344752; -.
DR   Ensembl; ENST00000356517.4; ENSP00000348911.3; ENSG00000197953.6. [Q6P093-1]
DR   Ensembl; ENST00000570799.1; ENSP00000461239.1; ENSG00000261846.2. [Q6P093-1]
DR   GeneID; 344752; -.
DR   KEGG; hsa:344752; -.
DR   MANE-Select; ENST00000356517.4; ENSP00000348911.3; NM_207365.4; NP_997248.2.
DR   UCSC; uc003ezc.4; human. [Q6P093-1]
DR   AGR; HGNC:24427; -.
DR   CTD; 344752; -.
DR   GeneCards; AADACL2; -.
DR   HGNC; HGNC:24427; AADACL2.
DR   HPA; ENSG00000197953; Tissue enriched (skin).
DR   neXtProt; NX_Q6P093; -.
DR   OpenTargets; ENSG00000197953; -.
DR   PharmGKB; PA142670463; -.
DR   VEuPathDB; HostDB:ENSG00000197953; -.
DR   eggNOG; KOG1515; Eukaryota.
DR   GeneTree; ENSGT00940000161405; -.
DR   HOGENOM; CLU_012494_12_0_1; -.
DR   InParanoid; Q6P093; -.
DR   OMA; DHVYTEP; -.
DR   OrthoDB; 1144477at2759; -.
DR   PhylomeDB; Q6P093; -.
DR   TreeFam; TF314978; -.
DR   PathwayCommons; Q6P093; -.
DR   SABIO-RK; Q6P093; -.
DR   BioGRID-ORCS; 344752; 13 hits in 1150 CRISPR screens.
DR   ChiTaRS; AADACL2; human.
DR   GenomeRNAi; 344752; -.
DR   Pharos; Q6P093; Tdark.
DR   PRO; PR:Q6P093; -.
DR   Proteomes; UP000005640; Chromosome 3.
DR   RNAct; Q6P093; Protein.
DR   Bgee; ENSG00000197953; Expressed in skin of abdomen and 40 other cell types or tissues.
DR   ExpressionAtlas; Q6P093; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013094; AB_hydrolase_3.
DR   InterPro; IPR017157; Arylacetamide_deacetylase.
DR   InterPro; IPR033140; Lipase_GDXG_put_SER_AS.
DR   PANTHER; PTHR23024; ARYLACETAMIDE DEACETYLASE; 1.
DR   PANTHER; PTHR23024:SF223; ARYLACETAMIDE DEACETYLASE-LIKE 2; 1.
DR   Pfam; PF07859; Abhydrolase_3; 2.
DR   PIRSF; PIRSF037251; Arylacetamide_deacetylase; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR   PROSITE; PS01174; LIPASE_GDXG_SER; 1.
DR   Genevisible; Q6P093; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Disulfide bond; Hydrolase; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..401
FT                   /note="Arylacetamide deacetylase-like 2"
FT                   /id="PRO_0000314960"
FT   MOTIF           111..113
FT                   /note="Involved in the stabilization of the negatively
FT                   charged intermediate by the formation of the oxyanion hole"
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        189
FT                   /evidence="ECO:0000250|UniProtKB:Q8BLF1,
FT                   ECO:0000255|PROSITE-ProRule:PRU10038"
FT   ACT_SITE        341
FT                   /evidence="ECO:0000250|UniProtKB:Q8BLF1"
FT   ACT_SITE        371
FT                   /evidence="ECO:0000250|UniProtKB:Q8BLF1"
FT   DISULFID        116..338
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         47..53
FT                   /note="AMCFENM -> NRGPLTS (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:17974005"
FT                   /id="VSP_038389"
FT   VAR_SEQ         54..401
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:17974005"
FT                   /id="VSP_038390"
FT   VARIANT         186
FT                   /note="A -> S (in dbSNP:rs1972977)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_038140"
FT   VARIANT         343
FT                   /note="L -> I (in dbSNP:rs1052562)"
FT                   /id="VAR_038141"
FT   CONFLICT        26
FT                   /note="N -> S (in Ref. 1; CAI46075)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   401 AA;  46099 MW;  801AF004D9E6A3DA CRC64;
     MGLKALCLGL LCVLFVSHFY TPMPDNIEES WKIMALDAIA KTCTFTAMCF ENMRIMRYEE
     FISMIFRLDY TQPLSDEYIT VTDTTFVDIP VRLYLPKRKS ETRRRAVIYF HGGGFCFGSS
     KQRAFDFLNR WTANTLDAVV VGVDYRLAPQ HHFPAQFEDG LAAVKFFLLE KILTKYGVDP
     TRICIAGDSS GGNLATAVTQ QVQNDAEIKH KIKMQVLLYP GLQITDSYLP SHRENEHGIV
     LTRDVAIKLV SLYFTKDEAL PWAMRRNQHM PLESRHLFKF VNWSILLPEK YRKDYVYTEP
     ILGGLSYSLP GLTDSRALPL LANDSQLQNL PLTYILTCQH DLLRDDGLMY VTRLRNVGVQ
     VVHEHIEDGI HGALSFMTSP FYLRLGLRIR DMYVSWLDKN L
//