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Protein

RNA pseudouridylate synthase domain-containing protein 3

Gene

RPUSD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • pseudouridine synthase activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
RNA pseudouridylate synthase domain-containing protein 3
Gene namesi
Name:RPUSD3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:28437. RPUSD3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134924883.

Polymorphism and mutation databases

BioMutaiRPUSD3.
DMDMi294862493.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351RNA pseudouridylate synthase domain-containing protein 3PRO_0000300822Add
BLAST

Proteomic databases

EPDiQ6P087.
MaxQBiQ6P087.
PaxDbiQ6P087.
PRIDEiQ6P087.

PTM databases

iPTMnetiQ6P087.
PhosphoSiteiQ6P087.

Expressioni

Gene expression databases

BgeeiQ6P087.
CleanExiHS_RPUSD3.
ExpressionAtlasiQ6P087. baseline and differential.
GenevisibleiQ6P087. HS.

Organism-specific databases

HPAiHPA037551.
HPA037552.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT31Q153233EBI-5458784,EBI-948001
KRTAP10-7P604093EBI-5458784,EBI-10172290

Protein-protein interaction databases

BioGridi130092. 26 interactions.
IntActiQ6P087. 6 interactions.
MINTiMINT-8052420.
STRINGi9606.ENSP00000373331.

Structurei

3D structure databases

ProteinModelPortaliQ6P087.
SMRiQ6P087. Positions 90-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase RluA family.Curated

Phylogenomic databases

eggNOGiKOG1919. Eukaryota.
COG0564. LUCA.
GeneTreeiENSGT00530000063800.
HOGENOMiHOG000082513.
HOVERGENiHBG098171.
InParanoidiQ6P087.
OMAiHLHCLHL.
PhylomeDBiQ6P087.
TreeFamiTF337899.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006145. PsdUridine_synth_RsuA/RluD.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6P087-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRAVLAREMD GRRVLGRFWS GWRRGLGVRP VPEDAGFGTE ARHQRQPRGS
60 70 80 90 100
CQRSGPLGDQ PFAGLLPKNL SREELVDALR AAVVDRKGPL VTLNKPQGLP
110 120 130 140 150
VTGKPGELTL FSVLPELSQS LGLREQELQV VRASGKESSG LVLLSSCPQT
160 170 180 190 200
ASRLQKYFTH ARRAQRPTAT YCAVTDGIPA ASEGKIQAAL KLEHIDGVNL
210 220 230 240 250
TVPVKAPSRK DILEGVKKTL SHFRVVATGS GCALVQLQPL TVFSSQLQVH
260 270 280 290 300
MVLQLCPVLG DHMYSARVGT VLGQRFLLPA ENNKPQRQVL DEALLRRLHL
310 320 330 340 350
TPSQAAQLPL HLHLHRLLLP GTRARDTPVE LLAPLPPYFS RTLQCLGLRL

Q
Length:351
Mass (Da):38,461
Last modified:April 20, 2010 - v3
Checksum:i7C308C9C56AC5C4F
GO
Isoform 2 (identifier: Q6P087-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-103: Missing.

Show »
Length:336
Mass (Da):36,945
Checksum:i08B64193A39A9F85
GO
Isoform 3 (identifier: Q6P087-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-351: Missing.

Show »
Length:136
Mass (Da):14,887
Checksum:iA77135FAE7C54D11
GO

Sequence cautioni

The sequence AAH62362.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH65741.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341D → H.1 Publication
Corresponds to variant rs17855991 [ dbSNP | Ensembl ].
VAR_034885
Natural varianti173 – 1731A → P.
Corresponds to variant rs34244989 [ dbSNP | Ensembl ].
VAR_059763
Natural varianti181 – 1811A → P.
Corresponds to variant rs34244989 [ dbSNP | Ensembl ].
VAR_034886

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei89 – 10315Missing in isoform 2. 1 PublicationVSP_027870Add
BLAST
Alternative sequencei137 – 351215Missing in isoform 3. 1 PublicationVSP_027869Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK092026 mRNA. Translation: BAC03794.1.
AK299556 mRNA. Translation: BAG61501.1.
AC018809 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64008.1.
BC032135 mRNA. Translation: AAH32135.1.
BC062362 mRNA. Translation: AAH62362.1. Different initiation.
BC065741 mRNA. Translation: AAH65741.1. Different initiation.
CCDSiCCDS2586.2. [Q6P087-1]
CCDS46744.1. [Q6P087-2]
RefSeqiNP_001136019.1. NM_001142547.1. [Q6P087-2]
NP_775930.2. NM_173659.3. [Q6P087-1]
UniGeneiHs.33719.

Genome annotation databases

EnsembliENST00000383820; ENSP00000373331; ENSG00000156990. [Q6P087-1]
ENST00000433535; ENSP00000398921; ENSG00000156990. [Q6P087-2]
GeneIDi285367.
KEGGihsa:285367.
UCSCiuc011atk.3. human. [Q6P087-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK092026 mRNA. Translation: BAC03794.1.
AK299556 mRNA. Translation: BAG61501.1.
AC018809 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64008.1.
BC032135 mRNA. Translation: AAH32135.1.
BC062362 mRNA. Translation: AAH62362.1. Different initiation.
BC065741 mRNA. Translation: AAH65741.1. Different initiation.
CCDSiCCDS2586.2. [Q6P087-1]
CCDS46744.1. [Q6P087-2]
RefSeqiNP_001136019.1. NM_001142547.1. [Q6P087-2]
NP_775930.2. NM_173659.3. [Q6P087-1]
UniGeneiHs.33719.

3D structure databases

ProteinModelPortaliQ6P087.
SMRiQ6P087. Positions 90-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130092. 26 interactions.
IntActiQ6P087. 6 interactions.
MINTiMINT-8052420.
STRINGi9606.ENSP00000373331.

PTM databases

iPTMnetiQ6P087.
PhosphoSiteiQ6P087.

Polymorphism and mutation databases

BioMutaiRPUSD3.
DMDMi294862493.

Proteomic databases

EPDiQ6P087.
MaxQBiQ6P087.
PaxDbiQ6P087.
PRIDEiQ6P087.

Protocols and materials databases

DNASUi285367.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000383820; ENSP00000373331; ENSG00000156990. [Q6P087-1]
ENST00000433535; ENSP00000398921; ENSG00000156990. [Q6P087-2]
GeneIDi285367.
KEGGihsa:285367.
UCSCiuc011atk.3. human. [Q6P087-1]

Organism-specific databases

CTDi285367.
GeneCardsiRPUSD3.
HGNCiHGNC:28437. RPUSD3.
HPAiHPA037551.
HPA037552.
neXtProtiNX_Q6P087.
PharmGKBiPA134924883.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1919. Eukaryota.
COG0564. LUCA.
GeneTreeiENSGT00530000063800.
HOGENOMiHOG000082513.
HOVERGENiHBG098171.
InParanoidiQ6P087.
OMAiHLHCLHL.
PhylomeDBiQ6P087.
TreeFamiTF337899.

Miscellaneous databases

GenomeRNAii285367.
PROiQ6P087.

Gene expression databases

BgeeiQ6P087.
CleanExiHS_RPUSD3.
ExpressionAtlasiQ6P087. baseline and differential.
GenevisibleiQ6P087. HS.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006145. PsdUridine_synth_RsuA/RluD.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Brain.
  2. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-351 (ISOFORM 2), VARIANT HIS-34.
    Tissue: Bone marrow, Brain and Eye.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRUSD3_HUMAN
AccessioniPrimary (citable) accession number: Q6P087
Secondary accession number(s): B4DS39
, Q6P6A9, Q8N1B2, Q8NAV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: April 20, 2010
Last modified: June 8, 2016
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.