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Q6P073

- UB2J2_MOUSE

UniProt

Q6P073 - UB2J2_MOUSE

Protein

Ubiquitin-conjugating enzyme E2 J2

Gene

Ube2j2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD).
    In case of infection by the murid herpesvirus 4, its association with the viral E3 ligase K3 mediates ubiquitination of host surface class I (MHC-I) H-2D(b)/H2-D1 and H-2K(b)/H2-K1 molecules before they exit the endoplasmic reticulum, leading to their degradation by the ERAD system, thus blocking the immune detection of virus-infected cells. The complex formed with the murid herpesvirus 4 protein K3 mediates ubiquitination of lysine, as well as serine and threonine residues present in the cytoplasmic tail of surface class I molecules and promotes ubiquitination of hydroxylated serine or threonine residues via ester bonds instead of the classical isopeptide linkage.

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei94 – 941Glycyl thioester intermediate

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway
    2. response to unfolded protein Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway, Unfolded protein response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 J2 (EC:6.3.2.19)
    Alternative name(s):
    Non-canonical ubiquitin-conjugating enzyme 2
    Short name:
    NCUBE-2
    Gene namesi
    Name:Ube2j2
    Synonyms:Ncube2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2153608. Ube2j2.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Single-pass type IV membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum Source: MGI
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi94 – 941C → S: Loss of catalytic activity. Slows down degradation of misfolded proteins from the ER. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 259259Ubiquitin-conjugating enzyme E2 J2PRO_0000082597Add
    BLAST

    Proteomic databases

    PaxDbiQ6P073.
    PRIDEiQ6P073.

    PTM databases

    PhosphoSiteiQ6P073.

    Expressioni

    Gene expression databases

    BgeeiQ6P073.
    GenevestigatoriQ6P073.

    Interactioni

    Subunit structurei

    Interacts with murid herpesvirus 4 protein K3 (mK3).1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ6P073.
    SMRiQ6P073. Positions 11-171.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 226226CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini248 – 25912LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei227 – 24721Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00530000063379.
    HOGENOMiHOG000170330.
    HOVERGENiHBG058434.
    InParanoidiQ6P073.
    KOiK04554.
    OrthoDBiEOG780RNH.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6P073-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNNSNKRAP TTATQRLKQD YLRIKKDPVP YICAEPLPSN ILEWHYVVRG    50
    PEMTPYEGGY YHGKLIFPRE FPFKPPSIYM ITPNGRFKCN TRLCLSITDF 100
    HPDTWNPAWS VSTILTGLLS FMVEKGPTLG SIETSDFTKK QLAAQSLVFN 150
    LKDKVFCELF PEVVEEIKQK QKAQDELSNR PQNLPLPDVV PDGELHRGQH 200
    GIQLLNGHAP AAGPNLAGLP QANRHHGLLG GALANLFVIV GFAAFAYTVK 250
    YVLRSIAQE 259
    Length:259
    Mass (Da):28,954
    Last modified:July 5, 2004 - v1
    Checksum:i0C539BC0ACEAC7C7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 251K → T in BAC36280. (PubMed:16141072)Curated
    Sequence conflicti32 – 321I → M in BAC36280. (PubMed:16141072)Curated
    Sequence conflicti49 – 491R → G in BAC36280. (PubMed:16141072)Curated
    Sequence conflicti80 – 801M → I in BAB26835. (PubMed:16141072)Curated
    Sequence conflicti84 – 841N → H in BAB26835. (PubMed:16141072)Curated
    Sequence conflicti87 – 871F → I in BAB26835. (PubMed:16141072)Curated
    Sequence conflicti133 – 1331E → K in BAB23421. (PubMed:16141072)Curated
    Sequence conflicti150 – 1501N → I in BAB26835. (PubMed:16141072)Curated
    Sequence conflicti157 – 1571C → G in BAC36280. (PubMed:16141072)Curated
    Sequence conflicti162 – 1621E → K in BAB23421. (PubMed:16141072)Curated
    Sequence conflicti186 – 1861L → W in BAC36280. (PubMed:16141072)Curated
    Sequence conflicti217 – 2182AG → GW in AAF21504. (PubMed:12082160)Curated
    Sequence conflicti237 – 2371F → L in BAC36280. (PubMed:16141072)Curated
    Sequence conflicti246 – 2461A → P in AAK52607. (PubMed:11278356)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296656 mRNA. Translation: AAK52607.1.
    U93242 mRNA. Translation: AAF21504.1.
    AK004626 mRNA. Translation: BAB23421.1.
    AK010302 mRNA. Translation: BAB26835.1.
    AK076265 mRNA. Translation: BAC36280.1.
    BC065779 mRNA. Translation: AAH65779.1.
    CCDSiCCDS19051.1.
    RefSeqiNP_001034246.1. NM_001039157.2.
    NP_001034247.1. NM_001039158.2.
    NP_001034248.1. NM_001039159.2.
    NP_001271241.1. NM_001284312.1.
    NP_001271243.1. NM_001284314.1.
    NP_067377.4. NM_021402.6.
    UniGeneiMm.371673.

    Genome annotation databases

    EnsembliENSMUST00000103175; ENSMUSP00000099464; ENSMUSG00000023286.
    ENSMUST00000105581; ENSMUSP00000101206; ENSMUSG00000023286.
    ENSMUST00000166489; ENSMUSP00000127712; ENSMUSG00000023286.
    GeneIDi140499.
    KEGGimmu:140499.
    UCSCiuc007ubh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF296656 mRNA. Translation: AAK52607.1 .
    U93242 mRNA. Translation: AAF21504.1 .
    AK004626 mRNA. Translation: BAB23421.1 .
    AK010302 mRNA. Translation: BAB26835.1 .
    AK076265 mRNA. Translation: BAC36280.1 .
    BC065779 mRNA. Translation: AAH65779.1 .
    CCDSi CCDS19051.1.
    RefSeqi NP_001034246.1. NM_001039157.2.
    NP_001034247.1. NM_001039158.2.
    NP_001034248.1. NM_001039159.2.
    NP_001271241.1. NM_001284312.1.
    NP_001271243.1. NM_001284314.1.
    NP_067377.4. NM_021402.6.
    UniGenei Mm.371673.

    3D structure databases

    ProteinModelPortali Q6P073.
    SMRi Q6P073. Positions 11-171.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6P073.

    Proteomic databases

    PaxDbi Q6P073.
    PRIDEi Q6P073.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103175 ; ENSMUSP00000099464 ; ENSMUSG00000023286 .
    ENSMUST00000105581 ; ENSMUSP00000101206 ; ENSMUSG00000023286 .
    ENSMUST00000166489 ; ENSMUSP00000127712 ; ENSMUSG00000023286 .
    GeneIDi 140499.
    KEGGi mmu:140499.
    UCSCi uc007ubh.1. mouse.

    Organism-specific databases

    CTDi 118424.
    MGIi MGI:2153608. Ube2j2.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00530000063379.
    HOGENOMi HOG000170330.
    HOVERGENi HBG058434.
    InParanoidi Q6P073.
    KOi K04554.
    OrthoDBi EOG780RNH.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 369836.
    PROi Q6P073.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6P073.
    Genevestigatori Q6P073.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s)."
      Tiwari S., Weissman A.M.
      J. Biol. Chem. 276:16193-16200(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-94, SUBCELLULAR LOCATION.
      Strain: C57BL/6J.
      Tissue: Fetus.
    2. "A role for mammalian Ubc6 homologues in ER-associated protein degradation."
      Lenk U., Yu H., Walter J., Gelman M.S., Hartmann E., Kopito R.R., Sommer T.
      J. Cell Sci. 115:3007-3014(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-94, SUBCELLULAR LOCATION.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Lung and Skin.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    5. "Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated degradation substrates."
      Wang X., Herr R.A., Rabelink M., Hoeben R.C., Wiertz E.J., Hansen T.H.
      J. Cell Biol. 187:655-668(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CASE OF INFECTION BY THE MURID HERPESVIRUS 4, INTERACTION WITH MURID HERPESVIRUS 4 K3.

    Entry informationi

    Entry nameiUB2J2_MOUSE
    AccessioniPrimary (citable) accession number: Q6P073
    Secondary accession number(s): Q8C6A1
    , Q91Y64, Q9CWY5, Q9DC18, Q9QX58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3