Q6P073 (UB2J2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 J2
EC=6.3.2.19

Alternative name(s):
Non-canonical ubiquitin-conjugating enzyme 2
Short name=NCUBE-2

Gene names

Name:	Ube2j2
Synonyms:	Ncube2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). Ref.1 Ref.2 Ref.5 In case of infection by the murid herpesvirus 4, its association with the viral E3 ligase K3 mediates ubiquitination of host surface class I (MHC-I) H-2D(b)/H2-D1 and H-2K(b)/H2-K1 molecules before they exit the endoplasmic reticulum, leading to their degradation by the ERAD system, thus blocking the immune detection of virus-infected cells. The complex formed with the murid herpesvirus 4 protein K3 mediates ubiquitination of lysine, as well as serine and threonine residues present in the cytoplasmic tail of surface class I molecules and promotes ubiquitination of hydroxylated serine or threonine residues via ester bonds instead of the classical isopeptide linkage. Ref.1 Ref.2 Ref.5
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with murid herpesvirus 4 protein K3 (mK3). Ref.5
Subcellular location	Endoplasmic reticulum membrane; Single-pass type IV membrane protein Ref.1 Ref.2.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Unfolded protein response
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to unfolded protein Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Ubiquitin-conjugating enzyme E2 J2		PRO_0000082597
Regions
	Topological domain	1 – 226	226	Cytoplasmic Potential
	Transmembrane	227 – 247	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	248 – 259	12	Lumenal Potential
Sites
	Active site	94	1	Glycyl thioester intermediate
Experimental info
	Mutagenesis	94	1	C → S: Loss of catalytic activity. Slows down degradation of misfolded proteins from the ER. Ref.1 Ref.2
	Sequence conflict	25	1	K → T in BAC36280. Ref.3
	Sequence conflict	32	1	I → M in BAC36280. Ref.3
	Sequence conflict	49	1	R → G in BAC36280. Ref.3
	Sequence conflict	80	1	M → I in BAB26835. Ref.3
	Sequence conflict	84	1	N → H in BAB26835. Ref.3
	Sequence conflict	87	1	F → I in BAB26835. Ref.3
	Sequence conflict	133	1	E → K in BAB23421. Ref.3
	Sequence conflict	150	1	N → I in BAB26835. Ref.3
	Sequence conflict	157	1	C → G in BAC36280. Ref.3
	Sequence conflict	162	1	E → K in BAB23421. Ref.3
	Sequence conflict	186	1	L → W in BAC36280. Ref.3
	Sequence conflict	217 – 218	2	AG → GW in AAF21504. Ref.2
	Sequence conflict	237	1	F → L in BAC36280. Ref.3
	Sequence conflict	246	1	A → P in AAK52607. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6P073 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0C539BC0ACEAC7C7	FASTA	259	28,954
	10 20 30 40 50 60 MSNNSNKRAP TTATQRLKQD YLRIKKDPVP YICAEPLPSN ILEWHYVVRG PEMTPYEGGY 70 80 90 100 110 120 YHGKLIFPRE FPFKPPSIYM ITPNGRFKCN TRLCLSITDF HPDTWNPAWS VSTILTGLLS 130 140 150 160 170 180 FMVEKGPTLG SIETSDFTKK QLAAQSLVFN LKDKVFCELF PEVVEEIKQK QKAQDELSNR 190 200 210 220 230 240 PQNLPLPDVV PDGELHRGQH GIQLLNGHAP AAGPNLAGLP QANRHHGLLG GALANLFVIV 250 GFAAFAYTVK YVLRSIAQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s)." Tiwari S., Weissman A.M. J. Biol. Chem. 276:16193-16200(2001) [PubMed: 11278356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-94, SUBCELLULAR LOCATION. Strain: C57BL/6J. Tissue: Fetus.
[2]	"A role for mammalian Ubc6 homologues in ER-associated protein degradation." Lenk U., Yu H., Walter J., Gelman M.S., Hartmann E., Kopito R.R., Sommer T. J. Cell Sci. 115:3007-3014(2002) [PubMed: 12082160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-94, SUBCELLULAR LOCATION.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung and Skin.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[5]	"Ube2j2 ubiquitinates hydroxylated amino acids on ER-associated degradation substrates." Wang X., Herr R.A., Rabelink M., Hoeben R.C., Wiertz E.J., Hansen T.H. J. Cell Biol. 187:655-668(2009) [PubMed: 19951915] [Abstract] Cited for: FUNCTION IN CASE OF INFECTION BY THE MURID HERPESVIRUS 4, INTERACTION WITH MURID HERPESVIRUS 4 K3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF296656 mRNA. Translation: AAK52607.1. U93242 mRNA. Translation: AAF21504.1. AK004626 mRNA. Translation: BAB23421.1. AK010302 mRNA. Translation: BAB26835.1. AK076265 mRNA. Translation: BAC36280.1. BC065779 mRNA. Translation: AAH65779.1.
IPI	IPI00420213.
RefSeq	NP_001034246.1. NM_001039157.1. NP_001034247.1. NM_001039158.1. NP_001034248.1. NM_001039159.1. NP_067377.4. NM_021402.5.
UniGene	Mm.371673.
3D structure databases
ProteinModelPortal	Q6P073.
SMR	Q6P073. Positions 11-171.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6P073.
Proteomic databases
PRIDE	Q6P073.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000103175; ENSMUSP00000099464; ENSMUSG00000023286. ENSMUST00000105581; ENSMUSP00000101206; ENSMUSG00000023286. ENSMUST00000166489; ENSMUSP00000127712; ENSMUSG00000023286.
GeneID	140499.
KEGG	mmu:140499.
Organism-specific databases
CTD	118424.
MGI	MGI:2153608. Ube2j2.
Phylogenomic databases
eggNOG	roNOG14047.
GeneTree	ENSGT00530000063379.
HOVERGEN	HBG058434.
InParanoid	Q6P073.
OrthoDB	EOG4BP1CG.
PhylomeDB	Q6P073.
Gene expression databases
ArrayExpress	Q6P073.
Bgee	Q6P073.
Genevestigator	Q6P073.
GermOnline	ENSMUSG00000023286. Mus musculus.
Family and domain databases
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
KO	K04554.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	369836.
SOURCE	Search...

Entry information

Entry name

UB2J2_MOUSE

Accession

Primary (citable) accession number: Q6P073
Secondary accession number(s): Q8C6A1 , Q91Y64, Q9CWY5, Q9DC18, Q9QX58

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program