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Protein

Ubiquitin-conjugating enzyme E2 J2

Gene

Ube2j2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD).1 Publication
In case of infection by the murid herpesvirus 4, its association with the viral E3 ligase K3 mediates ubiquitination of host surface class I (MHC-I) H-2D(b)/H2-D1 and H-2K(b)/H2-K1 molecules before they exit the endoplasmic reticulum, leading to their degradation by the ERAD system, thus blocking the immune detection of virus-infected cells. The complex formed with the murid herpesvirus 4 protein K3 mediates ubiquitination of lysine, as well as serine and threonine residues present in the cytoplasmic tail of surface class I molecules and promotes ubiquitination of hydroxylated serine or threonine residues via ester bonds instead of the classical isopeptide linkage.2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei94Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway, Unfolded protein response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 J2 (EC:2.3.2.23)
Alternative name(s):
Non-canonical ubiquitin-conjugating enzyme 2
Short name:
NCUBE-2
Gene namesi
Name:Ube2j2
Synonyms:Ncube2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2153608. Ube2j2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 226CytoplasmicSequence analysisAdd BLAST226
Transmembranei227 – 247Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini248 – 259LumenalSequence analysisAdd BLAST12

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94C → S: Loss of catalytic activity. Slows down degradation of misfolded proteins from the ER. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825971 – 259Ubiquitin-conjugating enzyme E2 J2Add BLAST259

Proteomic databases

EPDiQ6P073.
MaxQBiQ6P073.
PaxDbiQ6P073.
PRIDEiQ6P073.

PTM databases

PhosphoSitePlusiQ6P073.

Expressioni

Gene expression databases

BgeeiENSMUSG00000023286.
ExpressionAtlasiQ6P073. baseline and differential.
GenevisibleiQ6P073. MM.

Interactioni

Subunit structurei

Interacts with murid herpesvirus 4 protein K3 (mK3).1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024056.

Structurei

3D structure databases

ProteinModelPortaliQ6P073.
SMRiQ6P073.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0894. Eukaryota.
ENOG410Z0AN. LUCA.
GeneTreeiENSGT00530000063379.
HOGENOMiHOG000170330.
HOVERGENiHBG058434.
InParanoidiQ6P073.
KOiK04554.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6P073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNNSNKRAP TTATQRLKQD YLRIKKDPVP YICAEPLPSN ILEWHYVVRG
60 70 80 90 100
PEMTPYEGGY YHGKLIFPRE FPFKPPSIYM ITPNGRFKCN TRLCLSITDF
110 120 130 140 150
HPDTWNPAWS VSTILTGLLS FMVEKGPTLG SIETSDFTKK QLAAQSLVFN
160 170 180 190 200
LKDKVFCELF PEVVEEIKQK QKAQDELSNR PQNLPLPDVV PDGELHRGQH
210 220 230 240 250
GIQLLNGHAP AAGPNLAGLP QANRHHGLLG GALANLFVIV GFAAFAYTVK

YVLRSIAQE
Length:259
Mass (Da):28,954
Last modified:July 5, 2004 - v1
Checksum:i0C539BC0ACEAC7C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25K → T in BAC36280 (PubMed:16141072).Curated1
Sequence conflicti32I → M in BAC36280 (PubMed:16141072).Curated1
Sequence conflicti49R → G in BAC36280 (PubMed:16141072).Curated1
Sequence conflicti80M → I in BAB26835 (PubMed:16141072).Curated1
Sequence conflicti84N → H in BAB26835 (PubMed:16141072).Curated1
Sequence conflicti87F → I in BAB26835 (PubMed:16141072).Curated1
Sequence conflicti133E → K in BAB23421 (PubMed:16141072).Curated1
Sequence conflicti150N → I in BAB26835 (PubMed:16141072).Curated1
Sequence conflicti157C → G in BAC36280 (PubMed:16141072).Curated1
Sequence conflicti162E → K in BAB23421 (PubMed:16141072).Curated1
Sequence conflicti186L → W in BAC36280 (PubMed:16141072).Curated1
Sequence conflicti217 – 218AG → GW in AAF21504 (PubMed:12082160).Curated2
Sequence conflicti237F → L in BAC36280 (PubMed:16141072).Curated1
Sequence conflicti246A → P in AAK52607 (PubMed:11278356).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296656 mRNA. Translation: AAK52607.1.
U93242 mRNA. Translation: AAF21504.1.
AK004626 mRNA. Translation: BAB23421.1.
AK010302 mRNA. Translation: BAB26835.1.
AK076265 mRNA. Translation: BAC36280.1.
BC065779 mRNA. Translation: AAH65779.1.
CCDSiCCDS19051.1.
RefSeqiNP_001034246.1. NM_001039157.2.
NP_001034247.1. NM_001039158.2.
NP_001034248.1. NM_001039159.2.
NP_001271241.1. NM_001284312.1.
NP_001271243.1. NM_001284314.1.
NP_067377.4. NM_021402.6.
UniGeneiMm.371673.

Genome annotation databases

EnsembliENSMUST00000103175; ENSMUSP00000099464; ENSMUSG00000023286.
ENSMUST00000105581; ENSMUSP00000101206; ENSMUSG00000023286.
ENSMUST00000166489; ENSMUSP00000127712; ENSMUSG00000023286.
GeneIDi140499.
KEGGimmu:140499.
UCSCiuc007ubh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296656 mRNA. Translation: AAK52607.1.
U93242 mRNA. Translation: AAF21504.1.
AK004626 mRNA. Translation: BAB23421.1.
AK010302 mRNA. Translation: BAB26835.1.
AK076265 mRNA. Translation: BAC36280.1.
BC065779 mRNA. Translation: AAH65779.1.
CCDSiCCDS19051.1.
RefSeqiNP_001034246.1. NM_001039157.2.
NP_001034247.1. NM_001039158.2.
NP_001034248.1. NM_001039159.2.
NP_001271241.1. NM_001284312.1.
NP_001271243.1. NM_001284314.1.
NP_067377.4. NM_021402.6.
UniGeneiMm.371673.

3D structure databases

ProteinModelPortaliQ6P073.
SMRiQ6P073.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024056.

PTM databases

PhosphoSitePlusiQ6P073.

Proteomic databases

EPDiQ6P073.
MaxQBiQ6P073.
PaxDbiQ6P073.
PRIDEiQ6P073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103175; ENSMUSP00000099464; ENSMUSG00000023286.
ENSMUST00000105581; ENSMUSP00000101206; ENSMUSG00000023286.
ENSMUST00000166489; ENSMUSP00000127712; ENSMUSG00000023286.
GeneIDi140499.
KEGGimmu:140499.
UCSCiuc007ubh.1. mouse.

Organism-specific databases

CTDi118424.
MGIiMGI:2153608. Ube2j2.

Phylogenomic databases

eggNOGiKOG0894. Eukaryota.
ENOG410Z0AN. LUCA.
GeneTreeiENSGT00530000063379.
HOGENOMiHOG000170330.
HOVERGENiHBG058434.
InParanoidiQ6P073.
KOiK04554.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ6P073.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023286.
ExpressionAtlasiQ6P073. baseline and differential.
GenevisibleiQ6P073. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2J2_MOUSE
AccessioniPrimary (citable) accession number: Q6P073
Secondary accession number(s): Q8C6A1
, Q91Y64, Q9CWY5, Q9DC18, Q9QX58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.