Reviewed,
UniProtKB/Swiss-Prot Q6P073 (UB2J2_MOUSE)
Last modified
January 19, 2010.
Version 57.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ubiquitin-conjugating enzyme E2 J2 EC=6.3.2.19 Alternative name(s): Non-canonical ubiquitin-conjugating enzyme 2 Short name=NCUBE2 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 259 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum. Ref.1 Ref.2 |
| Catalytic activity | ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type IV membrane protein Ref.1 Ref.2. |
| Sequence similarities | Belongs to the ubiquitin-conjugating enzyme family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Ubl conjugation pathway |
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Gene Ontology (GO) | |
| Biological process | modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modificationInferred from electronic annotation. Source: InterPro regulation of protein metabolic processInferred from electronic annotation. Source: InterPro |
| Cellular component | endoplasmic reticulum Ref.1 Inferred from direct assay. Source: MGI integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 259 | 259 | Ubiquitin-conjugating enzyme E2 J2 | PRO_0000082597 | |||||
Regions | |||||||||
| Topological domain | 1 – 226 | 226 | Cytoplasmic Potential | ||||||
| Transmembrane | 227 – 247 | 21 | Anchor for type IV membrane protein Potential | ||||||
| Topological domain | 248 – 259 | 12 | Lumenal Potential | ||||||
Sites | |||||||||
| Active site | 94 | 1 | Glycyl thioester intermediate | ||||||
Experimental info | |||||||||
| Mutagenesis | 94 | 1 | C → S: Loss of catalytic activity. Slows down degradation of misfolded proteins from the ER. Ref.1 Ref.2 | ||||||
| Sequence conflict | 25 | 1 | K → T in BAC36280. Ref.3 | ||||||
| Sequence conflict | 32 | 1 | I → M in BAC36280. Ref.3 | ||||||
| Sequence conflict | 49 | 1 | R → G in BAC36280. Ref.3 | ||||||
| Sequence conflict | 80 | 1 | M → I in BAB26835. Ref.3 | ||||||
| Sequence conflict | 84 | 1 | N → H in BAB26835. Ref.3 | ||||||
| Sequence conflict | 87 | 1 | F → I in BAB26835. Ref.3 | ||||||
| Sequence conflict | 133 | 1 | E → K in BAB23421. Ref.3 | ||||||
| Sequence conflict | 150 | 1 | N → I in BAB26835. Ref.3 | ||||||
| Sequence conflict | 157 | 1 | C → G in BAC36280. Ref.3 | ||||||
| Sequence conflict | 162 | 1 | E → K in BAB23421. Ref.3 | ||||||
| Sequence conflict | 186 | 1 | L → W in BAC36280. Ref.3 | ||||||
| Sequence conflict | 217 – 218 | 2 | AG → GW in AAF21504. Ref.2 | ||||||
| Sequence conflict | 237 | 1 | F → L in BAC36280. Ref.3 | ||||||
| Sequence conflict | 246 | 1 | A → P in AAK52607. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s)." Tiwari S., Weissman A.M. J. Biol. Chem. 276:16193-16200(2001) [PubMed: 11278356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-94, SUBCELLULAR LOCATION. Strain: C57BL/6J. Tissue: Fetus. |
| [2] | "A role for mammalian Ubc6 homologues in ER-associated protein degradation." Lenk U., Yu H., Walter J., Gelman M.S., Hartmann E., Kopito R.R., Sommer T. J. Cell Sci. 115:3007-3014(2002) [PubMed: 12082160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-94, SUBCELLULAR LOCATION. |
| [3] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung and Skin. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF296656 mRNA. Translation: AAK52607.1. U93242 mRNA. Translation: AAF21504.1. AK004626 mRNA. Translation: BAB23421.1. AK010302 mRNA. Translation: BAB26835.1. AK076265 mRNA. Translation: BAC36280.1. BC065779 mRNA. Translation: AAH65779.1. |
| IPI | IPI00420213. |
| RefSeq | NP_001034246.1. NP_001034247.1. NP_001034248.1. NP_067377.4. |
| UniGene | Mm.371673 |
3D structure databases | |
| SMR | Q6P073. Positions 11-171. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q6P073. |
Proteomic databases | |
| PRIDE | Q6P073. |
Genome annotation databases | |
| Ensembl | ENSMUST00000024056; ENSMUSP00000024056; ENSMUSG00000023286; Mus musculus. [Genome view] ENSMUST00000103175; ENSMUSP00000099464; ENSMUSG00000023286; Mus musculus. [Genome view] ENSMUST00000105581; ENSMUSP00000101206; ENSMUSG00000023286; Mus musculus. [Genome view] ENSMUST00000105582; ENSMUSP00000101207; ENSMUSG00000023286; Mus musculus. [Genome view] ENSMUST00000105583; ENSMUSP00000101208; ENSMUSG00000023286; Mus musculus. [Genome view] |
| GeneID | 140499. |
| KEGG | mmu:140499. |
| UCSC | uc007ubh.1. mouse. |
Organism-specific databases | |
| CTD | 140499. |
| MGI | MGI:2153608. Ube2j2. |
Phylogenomic databases | |
| eggNOG | roNOG14047. |
| HOVERGEN | Q6P073. |
| InParanoid | Q6P073. |
| OrthoDB | EOG9XPT20. |
| PhylomeDB | Q6P073. |
Enzyme and pathway databases | |
| BRENDA | 6.3.2.19. 244. |
Gene expression databases | |
| ArrayExpress | Q6P073. |
| Genevestigator | Q6P073. |
| GermOnline | ENSMUSG00000023286. Mus musculus. |
Family and domain databases | |
| InterPro | IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view] |
| Gene3D | G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit. |
| Pfam | PF00179. UQ_con. 1 hit. [Graphical view] |
| SMART | SM00212. UBCc. 1 hit. [Graphical view] |
| PROSITE | PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 369836. |
| SOURCE | Search... |
Entry information
| Entry name | UB2J2_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q6P073 Secondary accession number(s): Q8C6A1 Q9QX58 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


