Q6NZY4 (ZCHC8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger CCHC domain-containing protein 8

Gene names

Name:

ZCCHC8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in pre-mRNA splicing.
Subunit structure	Identified in the spliceosome C complex. Ref.4
Sequence similarities	Belongs to the ZCCHC8 family. Contains 1 CCHC-type zinc finger.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Spliceosome
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred by curator Ref.4. Source: UniProtKB
Cellular_component	catalytic step 2 spliceosome Inferred from direct assay Ref.4. Source: UniProtKB
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRCA1	P38398	2	EBI-1263058,EBI-349905

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6NZY4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6NZY4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-238: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	Zinc finger CCHC domain-containing protein 8		PRO_0000150960
Regions
	Zinc finger	227 – 244	18	CCHC-type
	Coiled coil	45 – 80	36	Potential
	Coiled coil	516 – 539	24	Potential
	Compositional bias	466 – 501	36	Pro-rich
Amino acid modifications
	Modified residue	479	1	Phosphothreonine Ref.8
	Modified residue	485	1	Phosphothreonine Ref.8
	Modified residue	598	1	Phosphoserine Ref.6
	Modified residue	658	1	Phosphoserine Ref.6 Ref.7 Ref.8
Natural variations
	Alternative sequence	1 – 238	238	Missing in isoform 2.		VSP_013717
	Natural variant	672	1	P → A. Corresponds to variant rs1063155 [ dbSNP | Ensembl ].		VAR_034585
Experimental info
	Sequence conflict	20	1	E → G in BAB55308. Ref.1
	Sequence conflict	216 – 219	4	QEIQ → DAWV in AAH17704. Ref.3
	Sequence conflict	326	1	A → V in AAH65918. Ref.3
	Sequence conflict	461	1	S → G in BAB55308. Ref.1
	Sequence conflict	582	1	E → G in BAC11105. Ref.1
	Sequence conflict	663	1	M → V in CAB75658. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 7BE6E30F919A4771	FASTA	707	78,577
	10 20 30 40 50 60 MAAEVYFGDL ELFEPFDHPE ESIPKPVHTR FKDDDGDEED ENGVGDAELR ERLRQCEETI 70 80 90 100 110 120 EQLRAENQEL KRKLNILTRP SGILVNDTKL DGPILQILFM NNAISKQYHQ EIEEFVSNLV 130 140 150 160 170 180 KRFEEQQKND VEKTSFNLLP QPSSIVLEED HKVEESCAIK NNKEAFSVVG SVLYFTNFCL 190 200 210 220 230 240 DKLGQPLLNE NPQLSEGWEI PKYHQVFSHI VSLEGQEIQV KAKRPKPHCF NCGSEEHQMK 250 260 270 280 290 300 DCPMPRNAAR ISEKRKEYMD ACGEANNQNF QQRYHAEEVE ERFGRFKPGV ISEELQDALG 310 320 330 340 350 360 VTDKSLPPFI YRMRQLGYPP GWLKEAELEN SGLALYDGKD GTDGETEVGE IQQNKSVTYD 370 380 390 400 410 420 LSKLVNYPGF NISTPRGIPD EWRIFGSIPM QACQQKDVFA NYLTSNFQAP GVKSGNKRSS 430 440 450 460 470 480 SHSSPGSPKK QKNESNSAGS PADMELDSDM EVPHGSQSSE SFQFQPPLPP DTPPLPRGTP 490 500 510 520 530 540 PPVFTPPLPK GTPPLTPSDS PQTRTASGAV DEDALTLEEL EEQQRRIWAA LEQAESVNSD 550 560 570 580 590 600 SDVPVDTPLT GNSVASSPCP NELDLPVPEG KTSEKQTLDE PEVPEIFTKK SEAGHASSPD 610 620 630 640 650 660 SEVTSLCQKE KAELAPVNTE GALLDNGSVV PNCDISNGGS QKLFPADTSP STATKIHSPI 670 680 690 700 PDMSKFATGI TPFEFENMAE STGMYLRIRS LLKNSPRNQQ KNKKASE
	Isoform 2 [UniParc]. Checksum: 891FCBBB70026D4C	FASTA	469	51,130
	10 20 30 40 50 60 MKDCPMPRNA ARISEKRKEY MDACGEANNQ NFQQRYHAEE VEERFGRFKP GVISEELQDA 70 80 90 100 110 120 LGVTDKSLPP FIYRMRQLGY PPGWLKEAEL ENSGLALYDG KDGTDGETEV GEIQQNKSVT 130 140 150 160 170 180 YDLSKLVNYP GFNISTPRGI PDEWRIFGSI PMQACQQKDV FANYLTSNFQ APGVKSGNKR 190 200 210 220 230 240 SSSHSSPGSP KKQKNESNSA GSPADMELDS DMEVPHGSQS SESFQFQPPL PPDTPPLPRG 250 260 270 280 290 300 TPPPVFTPPL PKGTPPLTPS DSPQTRTASG AVDEDALTLE ELEEQQRRIW AALEQAESVN 310 320 330 340 350 360 SDSDVPVDTP LTGNSVASSP CPNELDLPVP EGKTSEKQTL DEPEVPEIFT KKSEAGHASS 370 380 390 400 410 420 PDSEVTSLCQ KEKAELAPVN TEGALLDNGS VVPNCDISNG GSQKLFPADT SPSTATKIHS 430 440 450 460 PIPDMSKFAT GITPFEFENM AESTGMYLRI RSLLKNSPRN QQKNKKASE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Duodenum.
[4]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Embryonic kidney.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-598 AND SER-658, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-658, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-479; THR-485 AND SER-658, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK027702 mRNA. Translation: BAB55308.1. AK074638 mRNA. Translation: BAC11105.1. AL157433 mRNA. Translation: CAB75657.1. AL157434 mRNA. Translation: CAB75658.1. BC017704 mRNA. Translation: AAH17704.2. BC065918 mRNA. Translation: AAH65918.1.
IPI	IPI00166422. IPI00556594.
PIR	T46929. T46930.
RefSeq	NP_060082.2. NM_017612.3.
UniGene	Hs.37706.
3D structure databases
ProteinModelPortal	Q6NZY4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6NZY4. 5 interactions.
MINT	MINT-1187494.
STRING	9606.ENSP00000337313.
PTM databases
PhosphoSite	Q6NZY4.
Polymorphism databases
DMDM	66774213.
Proteomic databases
PaxDb	Q6NZY4.
PRIDE	Q6NZY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336229; ENSP00000337313; ENSG00000033030. ENST00000536306; ENSP00000441423; ENSG00000033030. ENST00000543897; ENSP00000438993; ENSG00000033030.
GeneID	55596.
KEGG	hsa:55596.
UCSC	uc001ucm.3. human.
Organism-specific databases
CTD	55596.
GeneCards	GC12M122958.
H-InvDB	HIX0011095.
HGNC	HGNC:25265. ZCCHC8.
HPA	HPA037483. HPA037484.
neXtProt	NX_Q6NZY4.
PharmGKB	PA134889410.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG242619.
HOGENOM	HOG000155769.
HOVERGEN	HBG079371.
InParanoid	Q6NZY4.
KO	K13128.
OMA	FMNNAIS.
OrthoDB	EOG4P8FHV.
PhylomeDB	Q6NZY4.
Gene expression databases
ArrayExpress	Q6NZY4.
Bgee	Q6NZY4.
CleanEx	HS_ZCCHC8.
Genevestigator	Q6NZY4.
GermOnline	ENSG00000033030. Homo sapiens.
Family and domain databases
Gene3D	4.10.60.10. 1 hit.
InterPro	IPR006568. PSP. IPR001878. Znf_CCHC. [Graphical view]
Pfam	PF04046. PSP. 1 hit. PF00098. zf-CCHC. 1 hit. [Graphical view]
SMART	SM00581. PSP. 1 hit. SM00343. ZnF_C2HC. 1 hit. [Graphical view]
PROSITE	PS50158. ZF_CCHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55596.
NextBio	60130.
PMAP-CutDB	Q6NZY4.

Entry information

Entry name

ZCHC8_HUMAN

Accession

Primary (citable) accession number: Q6NZY4
Secondary accession number(s): Q7L2P6 , Q8N2K5, Q96SK7, Q9NSS2, Q9NSS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 10, 2005
Last modified:	April 3, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.