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Q6NZY4

- ZCHC8_HUMAN

UniProt

Q6NZY4 - ZCHC8_HUMAN

Protein

Zinc finger CCHC domain-containing protein 8

Gene

ZCCHC8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (10 May 2005)
      Previous versions | rss
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    Functioni

    May be involved in pre-mRNA splicing.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri227 – 24418CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: UniProtKB

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger CCHC domain-containing protein 8
    Alternative name(s):
    TRAMP-like complex RNA-binding factor ZCCHC8
    Gene namesi
    Name:ZCCHC8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:25265. ZCCHC8.

    Subcellular locationi

    Nucleusnucleoplasm 1 Publication
    Note: Excluded from nucleolus.

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: UniProtKB
    2. nucleoplasm Source: UniProtKB-SubCell
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134889410.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 707706Zinc finger CCHC domain-containing protein 8PRO_0000150960Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei479 – 4791Phosphothreonine1 Publication
    Modified residuei485 – 4851Phosphothreonine1 Publication
    Modified residuei598 – 5981Phosphoserine1 Publication
    Modified residuei658 – 6581Phosphoserine3 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ6NZY4.
    PaxDbiQ6NZY4.
    PRIDEiQ6NZY4.

    PTM databases

    PhosphoSiteiQ6NZY4.

    Miscellaneous databases

    PMAP-CutDBQ6NZY4.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6NZY4.
    BgeeiQ6NZY4.
    CleanExiHS_ZCCHC8.
    GenevestigatoriQ6NZY4.

    Organism-specific databases

    HPAiHPA037483.
    HPA037484.

    Interactioni

    Subunit structurei

    Component of a nuclear TRAMP-like complex, an ATP-dependent exosome regulatory complex consisting of an helicase (SKIV2L2/MTR4), an oligadenylate polymerase (PAPD5 or PAPD7), and a substrate specific RNA-binding factor (ZCCHC7 or ZCCHC8). Several TRAMP-like complexes exist with specific compositions and are associated with nuclear, or nucleolar RNA exosomes. Identified in the spliceosome C complex.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BRCA1P383982EBI-1263058,EBI-349905

    Protein-protein interaction databases

    BioGridi120739. 36 interactions.
    IntActiQ6NZY4. 8 interactions.
    MINTiMINT-1187494.
    STRINGi9606.ENSP00000337313.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6NZY4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili45 – 8036Sequence AnalysisAdd
    BLAST
    Coiled coili516 – 53924Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi466 – 50136Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ZCCHC8 family.Curated
    Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri227 – 24418CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG242619.
    HOGENOMiHOG000155769.
    HOVERGENiHBG079371.
    InParanoidiQ6NZY4.
    KOiK13128.
    OMAiFMNNAIS.
    OrthoDBiEOG7RNK09.
    PhylomeDBiQ6NZY4.
    TreeFamiTF321837.

    Family and domain databases

    Gene3Di4.10.60.10. 1 hit.
    InterProiIPR006568. PSP.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF04046. PSP. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view]
    SMARTiSM00581. PSP. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    PROSITEiPS50158. ZF_CCHC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6NZY4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAEVYFGDL ELFEPFDHPE ESIPKPVHTR FKDDDGDEED ENGVGDAELR    50
    ERLRQCEETI EQLRAENQEL KRKLNILTRP SGILVNDTKL DGPILQILFM 100
    NNAISKQYHQ EIEEFVSNLV KRFEEQQKND VEKTSFNLLP QPSSIVLEED 150
    HKVEESCAIK NNKEAFSVVG SVLYFTNFCL DKLGQPLLNE NPQLSEGWEI 200
    PKYHQVFSHI VSLEGQEIQV KAKRPKPHCF NCGSEEHQMK DCPMPRNAAR 250
    ISEKRKEYMD ACGEANNQNF QQRYHAEEVE ERFGRFKPGV ISEELQDALG 300
    VTDKSLPPFI YRMRQLGYPP GWLKEAELEN SGLALYDGKD GTDGETEVGE 350
    IQQNKSVTYD LSKLVNYPGF NISTPRGIPD EWRIFGSIPM QACQQKDVFA 400
    NYLTSNFQAP GVKSGNKRSS SHSSPGSPKK QKNESNSAGS PADMELDSDM 450
    EVPHGSQSSE SFQFQPPLPP DTPPLPRGTP PPVFTPPLPK GTPPLTPSDS 500
    PQTRTASGAV DEDALTLEEL EEQQRRIWAA LEQAESVNSD SDVPVDTPLT 550
    GNSVASSPCP NELDLPVPEG KTSEKQTLDE PEVPEIFTKK SEAGHASSPD 600
    SEVTSLCQKE KAELAPVNTE GALLDNGSVV PNCDISNGGS QKLFPADTSP 650
    STATKIHSPI PDMSKFATGI TPFEFENMAE STGMYLRIRS LLKNSPRNQQ 700
    KNKKASE 707
    Length:707
    Mass (Da):78,577
    Last modified:May 10, 2005 - v2
    Checksum:i7BE6E30F919A4771
    GO
    Isoform 2 (identifier: Q6NZY4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-238: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:469
    Mass (Da):51,130
    Checksum:i891FCBBB70026D4C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201E → G in BAB55308. (PubMed:14702039)Curated
    Sequence conflicti216 – 2194QEIQ → DAWV in AAH17704. (PubMed:15489334)Curated
    Sequence conflicti326 – 3261A → V in AAH65918. (PubMed:15489334)Curated
    Sequence conflicti461 – 4611S → G in BAB55308. (PubMed:14702039)Curated
    Sequence conflicti582 – 5821E → G in BAC11105. (PubMed:14702039)Curated
    Sequence conflicti663 – 6631M → V in CAB75658. (PubMed:17974005)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti672 – 6721P → A.
    Corresponds to variant rs1063155 [ dbSNP | Ensembl ].
    VAR_034585

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 238238Missing in isoform 2. 1 PublicationVSP_013717Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK027702 mRNA. Translation: BAB55308.1.
    AK074638 mRNA. Translation: BAC11105.1.
    AL157433 mRNA. Translation: CAB75657.1.
    AL157434 mRNA. Translation: CAB75658.1.
    BC017704 mRNA. Translation: AAH17704.2.
    BC065918 mRNA. Translation: AAH65918.1.
    PIRiT46929.
    T46930.
    RefSeqiNP_060082.2. NM_017612.4. [Q6NZY4-1]
    XP_005253638.1. XM_005253581.1. [Q6NZY4-2]
    XP_006719555.1. XM_006719492.1. [Q6NZY4-2]
    UniGeneiHs.37706.

    Genome annotation databases

    EnsembliENST00000536306; ENSP00000441423; ENSG00000033030. [Q6NZY4-2]
    ENST00000543897; ENSP00000438993; ENSG00000033030. [Q6NZY4-2]
    GeneIDi55596.
    KEGGihsa:55596.
    UCSCiuc001ucm.3. human. [Q6NZY4-1]

    Polymorphism databases

    DMDMi66774213.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK027702 mRNA. Translation: BAB55308.1 .
    AK074638 mRNA. Translation: BAC11105.1 .
    AL157433 mRNA. Translation: CAB75657.1 .
    AL157434 mRNA. Translation: CAB75658.1 .
    BC017704 mRNA. Translation: AAH17704.2 .
    BC065918 mRNA. Translation: AAH65918.1 .
    PIRi T46929.
    T46930.
    RefSeqi NP_060082.2. NM_017612.4. [Q6NZY4-1 ]
    XP_005253638.1. XM_005253581.1. [Q6NZY4-2 ]
    XP_006719555.1. XM_006719492.1. [Q6NZY4-2 ]
    UniGenei Hs.37706.

    3D structure databases

    ProteinModelPortali Q6NZY4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120739. 36 interactions.
    IntActi Q6NZY4. 8 interactions.
    MINTi MINT-1187494.
    STRINGi 9606.ENSP00000337313.

    PTM databases

    PhosphoSitei Q6NZY4.

    Polymorphism databases

    DMDMi 66774213.

    Proteomic databases

    MaxQBi Q6NZY4.
    PaxDbi Q6NZY4.
    PRIDEi Q6NZY4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000536306 ; ENSP00000441423 ; ENSG00000033030 . [Q6NZY4-2 ]
    ENST00000543897 ; ENSP00000438993 ; ENSG00000033030 . [Q6NZY4-2 ]
    GeneIDi 55596.
    KEGGi hsa:55596.
    UCSCi uc001ucm.3. human. [Q6NZY4-1 ]

    Organism-specific databases

    CTDi 55596.
    GeneCardsi GC12M122958.
    H-InvDB HIX0011095.
    HGNCi HGNC:25265. ZCCHC8.
    HPAi HPA037483.
    HPA037484.
    neXtProti NX_Q6NZY4.
    PharmGKBi PA134889410.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG242619.
    HOGENOMi HOG000155769.
    HOVERGENi HBG079371.
    InParanoidi Q6NZY4.
    KOi K13128.
    OMAi FMNNAIS.
    OrthoDBi EOG7RNK09.
    PhylomeDBi Q6NZY4.
    TreeFami TF321837.

    Miscellaneous databases

    GeneWikii ZCCHC8.
    GenomeRNAii 55596.
    NextBioi 60130.
    PMAP-CutDB Q6NZY4.
    PROi Q6NZY4.

    Gene expression databases

    ArrayExpressi Q6NZY4.
    Bgeei Q6NZY4.
    CleanExi HS_ZCCHC8.
    Genevestigatori Q6NZY4.

    Family and domain databases

    Gene3Di 4.10.60.10. 1 hit.
    InterProi IPR006568. PSP.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF04046. PSP. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view ]
    SMARTi SM00581. PSP. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    PROSITEi PS50158. ZF_CCHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Embryo.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain and Duodenum.
    4. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
      Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
      RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-598 AND SER-658, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-658, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-479; THR-485 AND SER-658, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION IN A TRAMP-LIKE COMPLEX, SUBUNIT, SUBCELLULAR LOCATION.
    12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Comparison of the yeast and human nuclear exosome complexes."
      Sloan K.E., Schneider C., Watkins N.J.
      Biochem. Soc. Trans. 40:850-855(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON RNA EXOSOMES.

    Entry informationi

    Entry nameiZCHC8_HUMAN
    AccessioniPrimary (citable) accession number: Q6NZY4
    Secondary accession number(s): Q7L2P6
    , Q8N2K5, Q96SK7, Q9NSS2, Q9NSS3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3