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Protein

Signal peptide, CUB and EGF-like domain-containing protein 1

Gene

Scube1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Signal peptide, CUB and EGF-like domain-containing protein 1
Gene namesi
Name:Scube1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1890616. Scube1.

Subcellular locationi

  • Secreted By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000025464921 – 1018Signal peptide, CUB and EGF-like domain-containing protein 1Add BLAST998

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 50By similarity
Disulfide bondi44 ↔ 59By similarity
Disulfide bondi61 ↔ 72By similarity
Disulfide bondi78 ↔ 91By similarity
Disulfide bondi87 ↔ 100By similarity
Disulfide bondi102 ↔ 115By similarity
Disulfide bondi121 ↔ 132By similarity
Disulfide bondi128 ↔ 141By similarity
Glycosylationi255N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi316 ↔ 327By similarity
Disulfide bondi323 ↔ 336By similarity
Disulfide bondi338 ↔ 351By similarity
Disulfide bondi357 ↔ 367By similarity
Disulfide bondi363 ↔ 376By similarity
Disulfide bondi378 ↔ 390By similarity
Disulfide bondi396 ↔ 407By similarity
Glycosylationi400N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi403 ↔ 416By similarity
Glycosylationi496N-linked (GlcNAc...)Sequence analysis1
Glycosylationi709N-linked (GlcNAc...)Sequence analysis1
Glycosylationi780N-linked (GlcNAc...)Sequence analysis1
Glycosylationi809N-linked (GlcNAc...)Sequence analysis1
Glycosylationi819N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi828 ↔ 854By similarity
Disulfide bondi881 ↔ 902By similarity

Post-translational modificationi

N-glycosylated.By similarity
Could be proteolytically cleaved to release a smaller active fragment.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ6NZL8.
PRIDEiQ6NZL8.

PTM databases

PhosphoSitePlusiQ6NZL8.

Expressioni

Developmental stagei

Expressed predominantly in the developing gonad, nervous system somites, surface ectoderm and limb buds.1 Publication

Inductioni

Down-regulated by inflammatory cytokines.

Gene expression databases

BgeeiENSMUSG00000016763.
CleanExiMM_SCUBE1.
ExpressionAtlasiQ6NZL8. baseline and differential.
GenevisibleiQ6NZL8. MM.

Interactioni

Subunit structurei

Forms homooligomers and heterooligomers with SCUBE2 and SCUBE3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ6NZL8. 1 interactor.
MINTiMINT-4653211.
STRINGi10090.ENSMUSP00000130131.

Structurei

3D structure databases

ProteinModelPortaliQ6NZL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 73EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini74 – 116EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST43
Domaini117 – 153EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini166 – 202EGF-like 4PROSITE-ProRule annotationAdd BLAST37
Domaini206 – 241EGF-like 5PROSITE-ProRule annotationAdd BLAST36
Domaini275 – 310EGF-like 6PROSITE-ProRule annotationAdd BLAST36
Domaini312 – 352EGF-like 7; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini353 – 391EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini392 – 428EGF-like 9; calcium-bindingPROSITE-ProRule annotationAdd BLAST37
Domaini828 – 940CUBPROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Contains 1 CUB domain.PROSITE-ProRule annotation
Contains 9 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IR7D. Eukaryota.
ENOG411030G. LUCA.
GeneTreeiENSGT00810000125382.
HOGENOMiHOG000230943.
HOVERGENiHBG054902.
InParanoidiQ6NZL8.
OMAiKYEGAVS.
OrthoDBiEOG091G00SV.
TreeFamiTF351672.

Family and domain databases

CDDicd00041. CUB. 1 hit.
Gene3Di2.60.120.290. 1 hit.
InterProiIPR026823. cEGF.
IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024731. EGF_dom.
IPR009030. Growth_fac_rcpt_.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
[Graphical view]
PfamiPF12662. cEGF. 1 hit.
PF00431. CUB. 1 hit.
PF12947. EGF_3. 1 hit.
PF07645. EGF_CA. 2 hits.
PF07699. Ephrin_rec_like. 3 hits.
[Graphical view]
SMARTiSM00042. CUB. 1 hit.
SM00181. EGF. 10 hits.
SM00179. EGF_CA. 7 hits.
SM01411. Ephrin_rec_like. 3 hits.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF57184. SSF57184. 3 hits.
PROSITEiPS00010. ASX_HYDROXYL. 6 hits.
PS01180. CUB. 1 hit.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
PS01187. EGF_CA. 6 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NZL8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAAAVRWHL YLLLALGARG RLVGGSGLPG AVDVDECSEG TDDCHIDAIC
60 70 80 90 100
QNTPKSYKCL CKPGYKGEGR QCEDIDECEN DYYNGGCVHD CINIPGNYRC
110 120 130 140 150
TCFDGFMLAH DGHNCLDVDE CQDNNGGCQQ ICVNAMGSYE CQCHSGFFLS
160 170 180 190 200
DNQHTCIHRS NEGMNCMNKD HGCAHICRET PKGGVACDCR PGFDLAQNQK
210 220 230 240 250
DCTLTCNYGN GGCQHSCEDT DTGPMCGCHQ KYALHADGRT CIEKDEAAIE
260 270 280 290 300
RSQFNATSVA DVDKRVKRRL LMETCAVNNG GCDRTCKDTA TGVRCSCPVG
310 320 330 340 350
FTLQPDGKTC KDINECLMNN GGCDHFCRNT VGSFECGCQK GHKLLTDERT
360 370 380 390 400
CQDIDECSFE RTCDHICINS PGSFQCLCRR GYTLYGTTHC GDVDECSMNN
410 420 430 440 450
GSCEQGCVNT KGSYECVCPP GRRLHWNQKD CVEMNGCLSR SKASAQAQLS
460 470 480 490 500
CGKVGGVENC FLSCLGHSLF MPDSESSYIL SCGVPGLQGK TLPKRNGTSS
510 520 530 540 550
STGPGCSDAP TTPIRQKARF KIRDAKCHLQ PRSQERAKDT LRHPLLDNCH
560 570 580 590 600
VTFVTLKCDS SKKRRRGRKS PSKEVSHITA EFEVEMKVDE ASGTCEADCM
610 620 630 640 650
RKRAEQSLQA AIKILRKSIG RNQFYVQVLG TEYEVAQRPA KALEGTGTCG
660 670 680 690 700
IGQILQDGKC VPCAPGTYFS GDPGQCMPCV SGTYQDMEGQ LSCTPCPSSE
710 720 730 740 750
GLGLAGARNV SECGGQCSPG YFSADGFKPC QACPVGTYQP EPGRTGCFPC
760 770 780 790 800
GGGLLTKHTG TASFQDCEAK VHCSPGHHYN TTTHRCIRCP VGTYQPEFGQ
810 820 830 840 850
NHCISCPGNT STDFDGSTNV THCKNQHCGG ELGDYTGYIE SPNYPGDYPA
860 870 880 890 900
NAECVWHIAP PPKRRILIVV PEIFLPIEDE CGDVLVMRKS ASPTSVTTYE
910 920 930 940 950
TCQTYERPIA FTSRSRKLWI QFKSNEANSG KGFQVPYVTY DEDYQQLIED
960 970 980 990 1000
IVRDGRLYAS ENHQEILKDK KLIKALFDVL AHPQNYFKYT AQESKEMFPR
1010
SFIKLLRSKV SRFLRPYK
Length:1,018
Mass (Da):111,606
Last modified:October 31, 2006 - v2
Checksum:i1F4B3EDF84F0CC42
GO
Isoform 2 (identifier: Q6NZL8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     244-273: Missing.

Show »
Length:988
Mass (Da):108,147
Checksum:iBB0789E800545961
GO
Isoform 3 (identifier: Q6NZL8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     244-273: Missing.
     942-991: EDYQQLIEDI...HPQNYFKYTA → GKSPPSCHSP...GLGNAETQGV
     992-1018: Missing.

Show »
Length:961
Mass (Da):104,078
Checksum:i21F9E4797CBB5A4D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11Y → S in AAG25939 (PubMed:11087664).Curated1
Sequence conflicti21R → Q in AAG25939 (PubMed:11087664).Curated1
Sequence conflicti411K → R in AAG25939 (PubMed:11087664).Curated1
Sequence conflicti619I → T in AAG25939 (PubMed:11087664).Curated1
Sequence conflicti833G → V in AAH66066 (PubMed:15489334).Curated1
Sequence conflicti941D → DGRLP in BAC30996 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021271244 – 273Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST30
Alternative sequenceiVSP_021272942 – 991EDYQQ…FKYTA → GKSPPSCHSPLCASQGLAWG LRNELHIPASDRAQTQRQKL GLGNAETQGV in isoform 3. 1 PublicationAdd BLAST50
Alternative sequenceiVSP_021273992 – 1018Missing in isoform 3. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276425 mRNA. Translation: AAG25939.1.
AK041590 mRNA. Translation: BAC30996.1.
BC066066 mRNA. Translation: AAH66066.1.
CCDSiCCDS49684.1. [Q6NZL8-2]
CCDS70655.1. [Q6NZL8-1]
RefSeqiNP_001258401.1. NM_001271472.1. [Q6NZL8-1]
NP_073560.2. NM_022723.3. [Q6NZL8-2]
UniGeneiMm.40393.

Genome annotation databases

EnsembliENSMUST00000016907; ENSMUSP00000016907; ENSMUSG00000016763. [Q6NZL8-1]
ENSMUST00000171496; ENSMUSP00000130131; ENSMUSG00000016763. [Q6NZL8-2]
GeneIDi64706.
KEGGimmu:64706.
UCSCiuc007xbi.2. mouse. [Q6NZL8-2]
uc007xbj.2. mouse. [Q6NZL8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF276425 mRNA. Translation: AAG25939.1.
AK041590 mRNA. Translation: BAC30996.1.
BC066066 mRNA. Translation: AAH66066.1.
CCDSiCCDS49684.1. [Q6NZL8-2]
CCDS70655.1. [Q6NZL8-1]
RefSeqiNP_001258401.1. NM_001271472.1. [Q6NZL8-1]
NP_073560.2. NM_022723.3. [Q6NZL8-2]
UniGeneiMm.40393.

3D structure databases

ProteinModelPortaliQ6NZL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6NZL8. 1 interactor.
MINTiMINT-4653211.
STRINGi10090.ENSMUSP00000130131.

PTM databases

PhosphoSitePlusiQ6NZL8.

Proteomic databases

PaxDbiQ6NZL8.
PRIDEiQ6NZL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016907; ENSMUSP00000016907; ENSMUSG00000016763. [Q6NZL8-1]
ENSMUST00000171496; ENSMUSP00000130131; ENSMUSG00000016763. [Q6NZL8-2]
GeneIDi64706.
KEGGimmu:64706.
UCSCiuc007xbi.2. mouse. [Q6NZL8-2]
uc007xbj.2. mouse. [Q6NZL8-1]

Organism-specific databases

CTDi80274.
MGIiMGI:1890616. Scube1.

Phylogenomic databases

eggNOGiENOG410IR7D. Eukaryota.
ENOG411030G. LUCA.
GeneTreeiENSGT00810000125382.
HOGENOMiHOG000230943.
HOVERGENiHBG054902.
InParanoidiQ6NZL8.
OMAiKYEGAVS.
OrthoDBiEOG091G00SV.
TreeFamiTF351672.

Miscellaneous databases

PROiQ6NZL8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016763.
CleanExiMM_SCUBE1.
ExpressionAtlasiQ6NZL8. baseline and differential.
GenevisibleiQ6NZL8. MM.

Family and domain databases

CDDicd00041. CUB. 1 hit.
Gene3Di2.60.120.290. 1 hit.
InterProiIPR026823. cEGF.
IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024731. EGF_dom.
IPR009030. Growth_fac_rcpt_.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
[Graphical view]
PfamiPF12662. cEGF. 1 hit.
PF00431. CUB. 1 hit.
PF12947. EGF_3. 1 hit.
PF07645. EGF_CA. 2 hits.
PF07699. Ephrin_rec_like. 3 hits.
[Graphical view]
SMARTiSM00042. CUB. 1 hit.
SM00181. EGF. 10 hits.
SM00179. EGF_CA. 7 hits.
SM01411. Ephrin_rec_like. 3 hits.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 1 hit.
SSF57184. SSF57184. 3 hits.
PROSITEiPS00010. ASX_HYDROXYL. 6 hits.
PS01180. CUB. 1 hit.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
PS01187. EGF_CA. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCUB1_MOUSE
AccessioniPrimary (citable) accession number: Q6NZL8
Secondary accession number(s): Q8C9Q4, Q9EQC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.