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Protein

Tonsoku-like protein

Gene

Tonsl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Tonsoku-like protein
Alternative name(s):
Inhibitor of kappa B-related protein
Short name:
I-kappa-B-related protein
Short name:
IkappaBR
NF-kappa-B inhibitor-like protein 2
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2
Gene namesi
Name:Tonsl
Synonyms:Ikbr, Nfkbil2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1919999. Tonsl.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13631363Tonsoku-like proteinPRO_0000326635Add
BLAST

Proteomic databases

EPDiQ6NZL6.
MaxQBiQ6NZL6.
PaxDbiQ6NZL6.
PeptideAtlasiQ6NZL6.
PRIDEiQ6NZL6.

PTM databases

iPTMnetiQ6NZL6.
PhosphoSiteiQ6NZL6.

Interactioni

Subunit structurei

Component of the MMS22L-TONSL complex, a complex at least composed of MMS22L and TONSL/NFKBIL2. Interacts with the MCM complex, the FACT complex and the RPA complex. Binds histones (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000129597.

Structurei

3D structure databases

ProteinModelPortaliQ6NZL6.
SMRiQ6NZL6. Positions 23-420, 488-665, 1004-1354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati27 – 6034TPR 1Add
BLAST
Repeati67 – 10034TPR 2Add
BLAST
Repeati107 – 14741TPR 3Add
BLAST
Repeati162 – 19534TPR 4Add
BLAST
Repeati202 – 23534TPR 5Add
BLAST
Repeati242 – 27534TPR 6Add
BLAST
Repeati311 – 34434TPR 7Add
BLAST
Repeati352 – 38534TPR 8Add
BLAST
Repeati528 – 55730ANK 1Add
BLAST
Repeati561 – 59030ANK 2Add
BLAST
Repeati597 – 62630ANK 3Add
BLAST
Repeati1060 – 108122LRR 1Add
BLAST
Repeati1088 – 110821LRR 2Add
BLAST
Repeati1119 – 114022LRR 3Add
BLAST
Repeati1147 – 116822LRR 4Add
BLAST
Repeati1179 – 119921LRR 5Add
BLAST
Repeati1206 – 12149LRR 6
Repeati1238 – 126124LRR 7Add
BLAST
Repeati1266 – 128722LRR 8Add
BLAST
Repeati1296 – 131722LRR 9Add
BLAST
Repeati1322 – 134322LRR 10Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi466 – 50944Glu-richAdd
BLAST

Domaini

The ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L.By similarity

Sequence similaritiesi

Belongs to the Tonsoku family.Curated
Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 10 LRR (leucine-rich) repeats.Curated
Contains 8 TPR repeats.Curated

Keywords - Domaini

ANK repeat, Leucine-rich repeat, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG4308. Eukaryota.
COG0666. LUCA.
HOVERGENiHBG107999.
InParanoidiQ6NZL6.
KOiK09257.
PhylomeDBiQ6NZL6.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
1.25.40.20. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF13516. LRR_6. 2 hits.
PF13176. TPR_7. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 3 hits.
SM00028. TPR. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF48452. SSF48452. 3 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NZL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLEQELRQL SKAKTRAQRN GQLREEAAYC HQLGELLASH GRFKDALEEH
60 70 80 90 100
QQELHLLESV QDTLGCAVAH RKIGERLAEM ENYSAALKHQ HLYLDLAGSL
110 120 130 140 150
SNHTELQRAW ATIGRTHLDI YDHCQSRDSL LQAQAAFEKS LAIVDEKLEG
160 170 180 190 200
MLTQRELSEM RTRLYLNLGL TCESLQQTAL CNNYFKKSIF LAEQNHLYED
210 220 230 240 250
LFRARYNLGA IHWRGGQHSQ AMRCLEGARE CARAMKMRFM ESECCVLVSQ
260 270 280 290 300
VLQDLGDFLA AKRALKKAYR LGSQKPNQRV TVCQSLKYVL AVIQLQQQLE
310 320 330 340 350
EAEGNDLQGA MAICEQLGDL FSKAGDFPKA AEAYQKQLHL AELLNRPDLE
360 370 380 390 400
LAVIHVSLAT TLGDMKDHRK AVHHYEEELR LRKGNALEEA KTWFNIALSR
410 420 430 440 450
EEAGDAYELL APCFQKAFCC AQQAQRFQLQ RQILQHLYTV QLKLQPQEAR
460 470 480 490 500
DTEIRLQELS MAKDTEEEEE EEEEEEEEAS EAPETSELEL SESEDDADGL
510 520 530 540 550
SQQLEEDEEL QGCVGRRKVN KWNRRNDMGE TLLHRACIEG QLRRVQDLVK
560 570 580 590 600
QGHPLNPRDY CGWTPLHEAC NYGHLEIVRF LLDHGAAVDD PGGQGCDGIT
610 620 630 640 650
PLHDALNCGH FEVAELLIER GASVTLRTRK GLSPLETLQQ WVKLYFRDLD
660 670 680 690 700
LETRQKAATM EERLQMASSG QASRSSPALQ TIPSNHLFDP ETSPPSSPCP
710 720 730 740 750
EPSSYTPRPP EASPAPAKVF LEETVSAVSR PRKTRHRPTS SSSSSEDEDN
760 770 780 790 800
PSPCRPSQKR LRHTTQQGEV KIPDPPKSRE TATSSACRAA YQAAIRGVGS
810 820 830 840 850
AQSRRLVPSL PRGSEEVPAP KTALIPEEEY LAGEWLEVDT PLTRSGRPST
860 870 880 890 900
SVSDYERCPA RPRTRVKQSR LTSLDGWCAR TQAGDGSLNA EPAENPSVPR
910 920 930 940 950
TSGPNKENYA AGQPLLLVQP PPIRVRVQIQ DNLFLIPVPQ SDIRPVAWLT
960 970 980 990 1000
EQAAQRYFQT CGLLPRLTLR KDGALLAPQD PIPDVLQSND EVLAEVTSWD
1010 1020 1030 1040 1050
LPPLKDRYRR ACLSLGQGEH QQVLHAMDHQ SSSPSFSACS LALCQAQLTP
1060 1070 1080 1090 1100
LLRALKLHTA LRELRLAGNR LGDACATELL ATLGTTPNLV LLDLSSNHLG
1110 1120 1130 1140 1150
QEGLRQLVEG SSGQAALQNL EELDLSMNPL GDGCGQALAS LLRACPMLST
1160 1170 1180 1190 1200
LRLQACGFSS SFFLSHQAAL GGAFQDAVHL KTLSLSYNLL GAPALARVLQ
1210 1220 1230 1240 1250
TLPACTLKRL DLSSVAASKS NSGIIEPVIK YLTKEGCALA HLTLSANCLG
1260 1270 1280 1290 1300
DKAVRELSRC LPCCPSLTSL DLSANPEVSC ASLEELLSAL QERSQGLSFL
1310 1320 1330 1340 1350
GLSGCSIQGP LNSDLWDKIF VQLQELQLCT KDLSTKDRDS VCQRLPEGAC
1360
TMDQSSKLFF KCL
Length:1,363
Mass (Da):151,108
Last modified:April 8, 2008 - v2
Checksum:i7216519012C69DB2
GO

Sequence cautioni

The sequence AAH66068 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC25399 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAC08218 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti293 – 2942IQ → NR in CAC08218 (Ref. 3) Curated
Sequence conflicti1106 – 11072QL → HV in BAC25399 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC156550 Genomic DNA. No translation available.
AC157566 Genomic DNA. No translation available.
BC066068 mRNA. Translation: AAH66068.1. Different initiation.
AJ294539 Genomic DNA. Translation: CAC08218.1. Sequence problems.
AK013272 mRNA. Translation: BAC25399.1. Different initiation.
CCDSiCCDS27580.2.
RefSeqiNP_898914.3. NM_183091.3.
UniGeneiMm.358749.

Genome annotation databases

GeneIDi72749.
KEGGimmu:72749.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC156550 Genomic DNA. No translation available.
AC157566 Genomic DNA. No translation available.
BC066068 mRNA. Translation: AAH66068.1. Different initiation.
AJ294539 Genomic DNA. Translation: CAC08218.1. Sequence problems.
AK013272 mRNA. Translation: BAC25399.1. Different initiation.
CCDSiCCDS27580.2.
RefSeqiNP_898914.3. NM_183091.3.
UniGeneiMm.358749.

3D structure databases

ProteinModelPortaliQ6NZL6.
SMRiQ6NZL6. Positions 23-420, 488-665, 1004-1354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000129597.

PTM databases

iPTMnetiQ6NZL6.
PhosphoSiteiQ6NZL6.

Proteomic databases

EPDiQ6NZL6.
MaxQBiQ6NZL6.
PaxDbiQ6NZL6.
PeptideAtlasiQ6NZL6.
PRIDEiQ6NZL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi72749.
KEGGimmu:72749.

Organism-specific databases

CTDi4796.
MGIiMGI:1919999. Tonsl.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG4308. Eukaryota.
COG0666. LUCA.
HOVERGENiHBG107999.
InParanoidiQ6NZL6.
KOiK09257.
PhylomeDBiQ6NZL6.

Miscellaneous databases

PROiQ6NZL6.
SOURCEiSearch...

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
1.25.40.20. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF13516. LRR_6. 2 hits.
PF13176. TPR_7. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 3 hits.
SM00028. TPR. 5 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF48452. SSF48452. 3 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTONSL_MOUSE
AccessioniPrimary (citable) accession number: Q6NZL6
Secondary accession number(s): Q8BT74, Q9ER45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: July 6, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.