Reviewed, UniProtKB/Swiss-Prot Q6NYS8 (OST48_DANRE)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
      Short name=Oligosaccharyl transferase 48 kDa subunit
      Short name=DDOST 48 kDa subunit
    EC=2.4.1.119

Gene names

Name:	ddost
ORF Names:	zgc:66068

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 441	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000357448
Regions
	Topological domain	29 – 410	382	Lumenal Potential
	Transmembrane	411 – 431	21	Potential
	Topological domain	432 – 441	10	Cytoplasmic Potential
Experimental info
	Sequence conflict	2	1	A → T in AAH56559. Ref.1
	Sequence conflict	13	1	A → P in AAH56559. Ref.1
	Sequence conflict	16	1	I → V in AAH56559. Ref.1
	Sequence conflict	72	1	I → L in AAH66475. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6NYS8-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 1D4E5C248F6948EE	FASTA	441	48,534
	10 20 30 40 50 60 MATALSGGFS KNALFILSAA LMLQAVLGDG KTLVLLDNPN IRDTHSIFFR SLADRGFDLT 70 80 90 100 110 120 FKTADDPGLS LIKYGQFLYD HLILFSPSVE DFGGNINVET ITAFIDGGGN VLVAASSDIG 130 140 150 160 170 180 DPLRELGSEC GIEFDEEKTA VIDHHNYDIS DPGEHTLIVA DPENLLKAPT IVGKPTDKPV 190 200 210 220 230 240 LFKGVGMVAD PDNPLVLDIL TGSSTSYSYF PDRPITQYPH AVGKNTLLIA GLQARNNARV 250 260 270 280 290 300 VFSGSLHFFS DAFFNSAVQK AATGSKRYEQ TGNQDLAEAL SRWVFKEAGV LRVGDVTHHP 310 320 330 340 350 360 VGESTPPAAY TVTDLVEYGI VIEMLSGGKW VPFDGDDIQL EFVRIDPFVR TYLKKNGGKY 370 380 390 400 410 420 SVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH TQYERFIPSA FPYYASAFSM 430 440 MAGLFVFSVV FLHMREKEKS D

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC056559 mRNA. Translation: AAH56559.1. BC066475 mRNA. Translation: AAH66475.1.
IPI	IPI00480914.
RefSeq	NP_998258.1.
UniGene	Dr.1142
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6NYS8.
Genome annotation databases
Ensembl	ENSDART00000054290; ENSDARP00000054289; ENSDARG00000037318; Danio rerio. [Genome view]
GeneID	406408.
KEGG	dre:406408.
Organism-specific databases
CTD	406408.
ZFIN	ZDB-GENE-040426-2147. ddost.
Phylogenomic databases
HOGENOM	Q6NYS8.
HOVERGEN	Q6NYS8.
Gene expression databases
ArrayExpress	Q6NYS8.
Bgee	Q6NYS8.
Family and domain databases
InterPro	IPR005013. DDOST_48kDa. [Graphical view]
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST48_DANRE

Accession

Primary (citable) accession number: Q6NYS8
Secondary accession number(s): Q6PHG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	October 13, 2009
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project