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Q6NYR8

- NPL_DANRE

UniProt

Q6NYR8 - NPL_DANRE

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Protein

N-acetylneuraminate lyase

Gene

npl

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway (By similarity).By similarity

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei143 – 1431Involved in proton transfer during cleavageBy similarity
Active sitei173 – 1731Schiff-base intermediate with substrateBy similarity

GO - Molecular functioni

  1. N-acetylneuraminate lyase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NALase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialate-pyruvate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:npl
ORF Names:zgc:76930
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-926. npl.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307N-acetylneuraminate lyasePRO_0000273358Add
BLAST

Proteomic databases

PRIDEiQ6NYR8.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6NYR8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 522Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.Curated

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ6NYR8.
PhylomeDBiQ6NYR8.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
PROSITEiPS00665. DHDPS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NYR8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQRVKKLTG LVAATFTPLT AEGEINLSVI AAYVDYLIEK QNVKSVFVNG
60 70 80 90 100
TTGEGCSLTV DERKHLAAAW CQHGKGKLEQ LIVHVGCMSI KDSQELARHA
110 120 130 140 150
ASIGADGISV ISPSYFKPIN ADALRLFIKE VSASAPDLPM YYYHLPGMTG
160 170 180 190 200
VALEAADVLN GIEREIPSFQ GVKYSGTDLR DLGQCVCYSQ SRDWSVLYGV
210 220 230 240 250
DEQLLGALVL GVHGAVGSTY NYLGHIVNQM LSAFNNGNHT QTRDLQFGFM
260 270 280 290 300
EVITFARTLG FDVSVNKQVM SEVSGLPMGP PRLPLLPCPV SKAQAIAQKI

HNFTQGL
Length:307
Mass (Da):33,221
Last modified:July 5, 2004 - v1
Checksum:i9E2222D36024CE5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC066486 mRNA. Translation: AAH66486.1.
UniGeneiDr.2954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC066486 mRNA. Translation: AAH66486.1 .
UniGenei Dr.2954.

3D structure databases

ProteinModelPortali Q6NYR8.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q6NYR8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ZFINi ZDB-GENE-030131-926. npl.

Phylogenomic databases

eggNOGi COG0329.
HOGENOMi HOG000218206.
HOVERGENi HBG082055.
InParanoidi Q6NYR8.
PhylomeDBi Q6NYR8.

Enzyme and pathway databases

UniPathwayi UPA00629 .

Miscellaneous databases

PROi Q6NYR8.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
PROSITEi PS00665. DHDPS_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiNPL_DANRE
AccessioniPrimary (citable) accession number: Q6NYR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3