Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6NYR8

- NPL_DANRE

UniProt

Q6NYR8 - NPL_DANRE

Protein

N-acetylneuraminate lyase

Gene

npl

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway By similarity.By similarity

    Catalytic activityi

    N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei143 – 1431Involved in proton transfer during cleavageBy similarity
    Active sitei173 – 1731Schiff-base intermediate with substrateBy similarity

    GO - Molecular functioni

    1. N-acetylneuraminate lyase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    UniPathwayiUPA00629.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylneuraminate lyase (EC:4.1.3.3)
    Short name:
    NALase
    Alternative name(s):
    N-acetylneuraminate pyruvate-lyase
    N-acetylneuraminic acid aldolase
    Sialate lyase
    Sialate-pyruvate lyase
    Sialic acid aldolase
    Sialic acid lyase
    Gene namesi
    Name:npl
    ORF Names:zgc:76930
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-030131-926. npl.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 307307N-acetylneuraminate lyasePRO_0000273358Add
    BLAST

    Proteomic databases

    PRIDEiQ6NYR8.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ6NYR8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni51 – 522Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DapA family. NanA subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0329.
    HOGENOMiHOG000218206.
    HOVERGENiHBG082055.
    InParanoidiQ6NYR8.
    PhylomeDBiQ6NYR8.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.
    PROSITEiPS00665. DHDPS_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6NYR8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQRVKKLTG LVAATFTPLT AEGEINLSVI AAYVDYLIEK QNVKSVFVNG    50
    TTGEGCSLTV DERKHLAAAW CQHGKGKLEQ LIVHVGCMSI KDSQELARHA 100
    ASIGADGISV ISPSYFKPIN ADALRLFIKE VSASAPDLPM YYYHLPGMTG 150
    VALEAADVLN GIEREIPSFQ GVKYSGTDLR DLGQCVCYSQ SRDWSVLYGV 200
    DEQLLGALVL GVHGAVGSTY NYLGHIVNQM LSAFNNGNHT QTRDLQFGFM 250
    EVITFARTLG FDVSVNKQVM SEVSGLPMGP PRLPLLPCPV SKAQAIAQKI 300
    HNFTQGL 307
    Length:307
    Mass (Da):33,221
    Last modified:July 5, 2004 - v1
    Checksum:i9E2222D36024CE5F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC066486 mRNA. Translation: AAH66486.1.
    UniGeneiDr.2954.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC066486 mRNA. Translation: AAH66486.1 .
    UniGenei Dr.2954.

    3D structure databases

    ProteinModelPortali Q6NYR8.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q6NYR8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ZFINi ZDB-GENE-030131-926. npl.

    Phylogenomic databases

    eggNOGi COG0329.
    HOGENOMi HOG000218206.
    HOVERGENi HBG082055.
    InParanoidi Q6NYR8.
    PhylomeDBi Q6NYR8.

    Enzyme and pathway databases

    UniPathwayi UPA00629 .

    Miscellaneous databases

    PROi Q6NYR8.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR002220. DapA-like.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    PROSITEi PS00665. DHDPS_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiNPL_DANRE
    AccessioniPrimary (citable) accession number: Q6NYR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3