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Q6NYN7 (S22A6_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 22 member 6
Alternative name(s):
Organic anion transporter 1
Renal organic anion transporter 1
Short name=ROAT1
Gene names
Name:slc22a6
Synonyms:oat
ORF Names:zgc:77073
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of p-aminohippurate (PAH), 2,3-dimercapto-1-propanesulfonic acid (DMPS), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), indomethacin, sulindac, diclofenac, carprofen, okadaic acid, benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, bumetamide, losartan, probenecid, phenol red, urate, glutarate and alpha-ketoglutarate By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Domain

Multiple cysteine residues are necessary for proper targeting to the plasma membrane By similarity.

Post-translational modification

Glycosylated. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane By similarity.

Sequence similarities

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Solute carrier family 22 member 6
PRO_0000324173

Regions

Topological domain1 – 1515Cytoplasmic Potential
Transmembrane16 – 3621Helical; Potential
Topological domain37 – 143107Extracellular Potential
Transmembrane144 – 16421Helical; Potential
Topological domain165 – 1706Cytoplasmic Potential
Transmembrane171 – 19121Helical; Potential
Topological domain192 – 20110Extracellular Potential
Transmembrane202 – 22221Helical; Potential
Topological domain223 – 2286Cytoplasmic Potential
Transmembrane229 – 24921Helical; Potential
Topological domain250 – 2567Extracellular Potential
Transmembrane257 – 27721Helical; Potential
Topological domain278 – 34568Cytoplasmic Potential
Transmembrane346 – 36621Helical; Potential
Topological domain367 – 3748Extracellular Potential
Transmembrane375 – 39521Helical; Potential
Topological domain396 – 40611Cytoplasmic Potential
Transmembrane407 – 42721Helical; Potential
Topological domain428 – 4336Extracellular Potential
Transmembrane434 – 45421Helical; Potential
Topological domain455 – 46511Cytoplasmic Potential
Transmembrane466 – 48621Helical; Potential
Topological domain487 – 4915Extracellular Potential
Transmembrane492 – 51221Helical; Potential
Topological domain513 – 56048Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
Q6NYN7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 14D3F32AB9771D45

FASTA56061,972
        10         20         30         40         50         60 
MAFSDLLEQV GSTGRFQVLH VTLLSMPILM MASHNLLQNF VAAVPPHHCQ PHANLSMSST 

        70         80         90        100        110        120 
DAVDVLRATV PLGLNGKLER CKRYTTPQWQ ILSLNTSESL WDESMAETET QSCEDGWFYN 

       130        140        150        160        170        180 
MTEMSSTIIT EWDLVCDYRA LKQMSQTTYM GGVLVGAIVF GGLSDRFGRR VLLLISNLMM 

       190        200        210        220        230        240 
AIGGTCVAFS TSFTMFCVFR VCCGMALSGL VLNSFSLIVE WIPTRVRTVV GTGTGYCYTT 

       250        260        270        280        290        300 
GQLILAAVAY CIRDWRWLTL AVSLPFYVSF LYSWWFLESA RWLVLTKNPE QAVKNLKTVA 

       310        320        330        340        350        360 
RINGRSAEGD KIDLEMLQES MKKEMACSKG SYSALDLLRT STMRTITICL SAVWFSTSFA 

       370        380        390        400        410        420 
YYGLSMDLQK FGVSIYLIQI IFGAVDIPAK IIVTICMSML GRRPSQCGAL VLAGIMILIN 

       430        440        450        460        470        480 
LLVPSDLQML RTSLAVIGKG CLAASFNCCY LYAGELYPTV IRQSGMGWVS MMARFGAMVA 

       490        500        510        520        530        540 
PMVLLLGDDY PWIPGFIYGG APIVSGIFAF FLPETLSQPL PDTIQDIDDR GLARTNSKRL 

       550        560 
PEKLDLAMKD PSCVLLKESV 

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC066519 mRNA. Translation: AAH66519.1.
RefSeqNP_996960.1. NM_207077.1.
UniGeneDr.107265.

3D structure databases

ProteinModelPortalQ6NYN7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000072353.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID404609.
KEGGdre:404609.

Organism-specific databases

CTD4942.
ZFINZDB-GENE-040426-2249. slc22a6l.

Phylogenomic databases

eggNOGCOG0477.
HOGENOMHOG000234569.
HOVERGENHBG108433.
InParanoidQ6NYN7.
KOK08203.
PhylomeDBQ6NYN7.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20817677.

Entry information

Entry nameS22A6_DANRE
AccessionPrimary (citable) accession number: Q6NYN7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families