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Protein

Peroxisomal bifunctional enzyme

Gene

ehhadh

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100Substrate; via amide nitrogenBy similarity1
Sitei123Important for catalytic activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandNAD

Enzyme and pathway databases

ReactomeiR-DRE-390247 Beta-oxidation of very long chain fatty acids
R-DRE-9033241 Peroxisomal protein import
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal bifunctional enzyme
Short name:
PBE
Short name:
PBFE
Including the following 2 domains:
Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase (EC:4.2.1.17, EC:5.3.3.8)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:ehhadh
Synonyms:echd
ORF Names:si:dkeyp-30d5.2, zgc:77526
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi

Organism-specific databases

ZFINiZDB-GENE-040426-2581 ehhadh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003531822 – 718Peroxisomal bifunctional enzymeAdd BLAST717

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6NYL3
PRIDEiQ6NYL3

Expressioni

Gene expression databases

BgeeiENSDARG00000070029

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000093211

Structurei

3D structure databases

ProteinModelPortaliQ6NYL3
SMRiQ6NYL3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 280Enoyl-CoA hydratase / isomeraseAdd BLAST279
Regioni281 – 5673-hydroxyacyl-CoA dehydrogenaseAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi716 – 718Microbody targeting signalBy similarity3

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.Curated

Phylogenomic databases

eggNOGiKOG1683 Eukaryota
COG1250 LUCA
GeneTreeiENSGT00880000137923
HOGENOMiHOG000261347
HOVERGENiHBG104990
InParanoidiQ6NYL3
KOiK07514
OMAiIDLCMIL
OrthoDBiEOG091G082G
PhylomeDBiQ6NYL3
TreeFamiTF316708

Family and domain databases

InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR001753 Enoyl-CoA_hydra/iso
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit
PS00166 ENOYL_COA_HYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6NYL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARYELVKRS VALITLTNPP VNALSSAVRH AISKTMERAL SDPKVTAVVI
60 70 80 90 100
CGENGRFCGG ADIREFAGPL RGPPLVPLLD AIEAGEKPVV AAIEGVALGG
110 120 130 140 150
GFELALVCHY RIAHYKARLG LPEVTLGILP AAGGTQRLPR LIGIPAALEL
160 170 180 190 200
ITTGRHVSAQ EALKLGMVDQ VTEQNTCEVA LEFALKAVGK PLSSRRLSML
210 220 230 240 250
TTPCPPGLDG IFEAATMQVQ KKARGVMAPL ACVQAVRAAT LPYSEGIKRE
260 270 280 290 300
GELMATLFSS GQAQALQYSF FAQRTAEKWT LPSGAQWNNS KPREIQSAAV
310 320 330 340 350
IGLGTMGRGI VVSLARVGIS VIAVESEKKL LETGRQMVIG MLERDAKRRG
360 370 380 390 400
VSASLNLLKF SLSLQDLKDV DLVIEAVFED MALKKQIFRE LSRVCRPATL
410 420 430 440 450
LCSNTSGLDV DALADVTDRP QLVAGMHFFS PAHVMKLLEV VCGPRSSKEA
460 470 480 490 500
IATAMSLGKR MGKVSVAVGN CPGFVGNRML MPYLEQATFL LEEGATPQQI
510 520 530 540 550
DKALEDFGFA MGVFRMSDLA GLDVGWRVRK ESGLTGPDVD PKDPPRRRQG
560 570 580 590 600
RKYCPIPDMV CQQGRFGQKT GRGWYMYDKP GDTNAKPDPL IQNLLETYRS
610 620 630 640 650
RYGIQPRKIT DQEIIERCLF ALANEGFRIL KDKIAGQPED IDVIYLFGYG
660 670 680 690 700
FPRHRGGPMF YASMVGLERV LERLEYYHHA LPDVPHLEPS PLLKKLVARG
710
SPPIQKWREH IKSMHSHL
Length:718
Mass (Da):78,508
Last modified:July 5, 2004 - v1
Checksum:iEA4DC2FDDCAE95B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR936497 Genomic DNA Translation: CAP19438.1
BC066545 mRNA Translation: AAH66545.1
RefSeqiNP_996951.1, NM_207068.1
UniGeneiDr.80045

Genome annotation databases

EnsembliENSDART00000102434; ENSDARP00000093211; ENSDARG00000070029
GeneIDi100000859
KEGGidre:100000859

Entry informationi

Entry nameiECHP_DANRE
AccessioniPrimary (citable) accession number: Q6NYL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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