Reviewed, UniProtKB/Swiss-Prot Q6NYG8 (DPYD_DANRE)

Names and origin

Protein names

Recommended name:
    Dihydropyrimidine dehydrogenase [NADP+]
      Short name=DHPDHase
      Short name=DPD
    EC=1.3.1.2
Alternative name(s):
    Dihydrouracil dehydrogenase
    Dihydrothymine dehydrogenase

Gene names

Name:	dpyd
ORF Names:	zgc:77205

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	1022 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine By similarity.
Catalytic activity	5,6-dihydrouracil + NADP+ = uracil + NADPH.
Cofactor	Binds 2 4Fe-4S clusters By similarity. FAD By similarity. FMN By similarity.
Pathway	Amino-acid biosynthesis; beta-alanine biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the dihydropyrimidine dehydrogenase family. Contains 3 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	4Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW thymidine catabolic process Inferred from sequence or structural similarity. Source: UniProtKB uracil catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro dihydropyrimidine dehydrogenase (NADP+) activity Inferred from sequence or structural similarity. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1022	1022	Dihydropyrimidine dehydrogenase [NADP+]		PRO_0000327504
Regions
	Domain	69 – 100	32	4Fe-4S ferredoxin-type 1
	Domain	943 – 975	33	4Fe-4S ferredoxin-type 2
	Domain	976 – 1006	31	4Fe-4S ferredoxin-type 3
Sites
	Metal binding	952	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	955	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	958	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	962	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	985	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	988	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	991	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	995	1	Iron-sulfur 2 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6NYG8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8A8983492F936654	FASTA	1,022	111,004
	10 20 30 40 50 60 MATMLSKELQ DIESILALNP RVKSHANVHS TASKKNEKKH WKRNPERSCD SCVNLENNFD 70 80 90 100 110 120 DIKHTTLSER GALREALRCL KCADAPCQKS CPTNLDIKSF ITSISNKNYY GAAKAILSDN 130 140 150 160 170 180 PLGLTCGMVC PTSDLCVGGC NLYASEEGPI NIGGLQQFAT EVFSKMGIPQ IRNPELPTAD 190 200 210 220 230 240 NMPKSFHTRI ALIGCGPASI SCASFLARLG YDNITIFEKQ KYIGGLSTSE IPQFRLPYEV 250 260 270 280 290 300 VQFEIDLMKD LGVKVVLEKG LGQNGLTLTS LKEEGYQVVY IGIGLPQANR DKIFEGLTTE 310 320 330 340 350 360 QGFYTSKDFL PLVAKASKIG MCNCRSQLPK LHGNVIVLGA GDTAFDCATS ALRCGARRVF 370 380 390 400 410 420 VVFRKGFTNI RAVPEEMEAA KEEKCEFLPF LSPHEVIKKN GRVSGLRFCR TEQQDDGTWI 430 440 450 460 470 480 VDEEQIVHLK ADFIISAFGS MLNDPAVTKA LDPIKLNRWG TPEVNSETMQ TTEPWVFAGG 490 500 510 520 530 540 DIAGFANTTV ESVNDGKQAS WHIHKYIQSL HGNTISATPR LPLFHCSIDT VDISVEMCGI 550 560 570 580 590 600 KFPNPFGLAS APPTTSAAMI RRAFEQGWGF ALTKTFGLDK DLVTNVSPRI VRGTTSGHIF 610 620 630 640 650 660 GPGQGSFLNI ELISEKTAAY WCKSVAELKA DFPKNIIIAS IMCSYNQADW TELAKMAQES 670 680 690 700 710 720 QADALELNLS CPHGMGERGM GLACGQDPEL VRNICRWVRK ATSIPFFAKL TPNVTNIVDI 730 740 750 760 770 780 ATAAYEGGAD GVTATNTVSG LMALKADATP WPGIGRGART TYGGVSGNAI RPIALRAVSA 790 800 810 820 830 840 IARALPGFPI LATGGIDSAE SGLQFLHAGA SVLQVCSAVQ NQDFTVIEDY CLGLKALLYL 850 860 870 880 890 900 KSIEELHDWD GQSPPTIRHQ KGKPVPHVSE LIGKSLPSFG PYLQTKTQAL AKYKKDASGD 910 920 930 940 950 960 VIMDTGAARV NIPKKPIPNV KDVIARALKH IGAYQELDNT EQVQALVDPE MCINCGKCYM 970 980 990 1000 1010 1020 TCNDSGYQAI KFDPETHLPV ITDSCTGCTL CLSVCPIIDC IKMVSRTTPY EPKRGLPVNP VC

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC066602 mRNA. Translation: AAH66602.1.
IPI	IPI00502639.
RefSeq	NP_998058.1.
UniGene	Dr.79932
3D structure databases
SMR	Q6NYG8. Positions 2-1016.
ModBase	Search...
Proteomic databases
PRIDE	Q6NYG8.
Genome annotation databases
Ensembl	ENSDARG00000010267. Danio rerio. [Contig view]
GeneID	405829.
KEGG	dre:405829.
Organism-specific databases
ZFIN	ZDB-GENE-040426-2459. dpyd.
Phylogenomic databases
HOGENOM	Q6NYG8.
HOVERGEN	Q6NYG8.
Enzyme and pathway databases
BRENDA	1.3.1.2. 96826.
Gene expression databases
ArrayExpress	Q6NYG8.
Bgee	Q6NYG8.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR000759. Adrndx_reductase. IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005720. Dihydroorotate_DH_1_core. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR012285. Fum_reductase_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00419. ADXRDTASE.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPYD_DANRE

Accession

Primary (citable) accession number: Q6NYG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project