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Q6NYB7 (RAB1A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-1A
Gene names
Name:Rab1A
Synonyms:Rab1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length205 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in anterograde melanosome transport By similarity. Plays a role in microtubule-dependent protein transport by early endosomes. Ref.4

Subunit structure

May interact with YIPF5. Interacts with GDI1; this promotes dissociation from membranes By similarity.

Subcellular location

Golgi apparatus By similarity. Endoplasmic reticulum By similarity. Early endosome. Cytoplasmcytosol By similarity. Membrane By similarity. Melanosome By similarity. Note: Alternates between membrane-associated and cytosolic forms By similarity. Ref.4

Tissue specificity

Expressed in flagella of epididymal sperm. Ref.3

Post-translational modification

Phosphorylated by CDK1 kinase during mitosis By similarity.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Biological processAutophagy
ER-Golgi transport
Protein transport
Transport
   Cellular componentCytoplasm
Endoplasmic reticulum
Endosome
Golgi apparatus
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMAcetylation
Lipoprotein
Phosphoprotein
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processER to Golgi vesicle-mediated transport

Inferred from direct assay PubMed 15728249. Source: MGI

GTP catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

Rab protein signal transduction

Inferred by curator PubMed 8807639. Source: RGD

autophagic vacuole assembly

Inferred from sequence or structural similarity. Source: UniProtKB

autophagy

Inferred from sequence or structural similarity. Source: UniProtKB

cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

defense response to bacterium

Inferred from sequence or structural similarity. Source: UniProtKB

endocytosis

Inferred from mutant phenotype Ref.4. Source: UniProtKB

growth hormone secretion

Inferred from sequence or structural similarity. Source: UniProtKB

interleukin-8 secretion

Inferred from sequence or structural similarity. Source: UniProtKB

vesicle transport along microtubule

Inferred from mutant phenotype Ref.4. Source: UniProtKB

   Cellular_componentGolgi membrane

Inferred from direct assay PubMed 8807639. Source: RGD

cytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

early endosome

Inferred from direct assay Ref.4. Source: UniProtKB

endoplasmic reticulum membrane

Inferred from direct assay PubMed 8807639. Source: RGD

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

neuronal cell body

Inferred from direct assay PubMed 8573789. Source: RGD

   Molecular_functionGTP binding

Inferred from direct assay PubMed 8807639. Source: RGD

GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 205204Ras-related protein Rab-1A
PRO_0000121059

Regions

Nucleotide binding18 – 269GTP By similarity
Nucleotide binding36 – 438GTP By similarity
Nucleotide binding66 – 705GTP By similarity
Nucleotide binding124 – 1274GTP By similarity
Nucleotide binding154 – 1563GTP By similarity
Motif40 – 489Effector region By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue791O-(2-cholinephosphoryl)serine By similarity
Modified residue1941Phosphoserine; by CDK1 By similarity
Lipidation2041S-geranylgeranyl cysteine By similarity
Lipidation2051S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict158 – 1592AT → EK in AAA42006. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6NYB7 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B2A8F4E3B0FB17D6

FASTA20522,678
        10         20         30         40         50         60 
MSSMNPEYDY LFKLLLIGDS GVGKSCLLLR FADDTYTESY ISTIGVDFKI RTIELDGKTI 

        70         80         90        100        110        120 
KLQIWDTAGQ ERFRTITSSY YRGAHGIIVV YDVTDQESFN NVKQWLQEID RYASENVNKL 

       130        140        150        160        170        180 
LVGNKCDLTT KKVVDYTTAK EFADSLGIPF LETSAKNATN VEQSFMTMAA EIKKRMGPGA 

       190        200 
TAGGAEKSNV KIQSTPVKQS GGGCC 

« Hide

References

« Hide 'large scale' references
[1]"Four additional members of the ras gene superfamily isolated by an oligonucleotide strategy: molecular cloning of YPT-related cDNAs from a rat brain library."
Touchot N., Chardin P., Tavitian A.
Proc. Natl. Acad. Sci. U.S.A. 84:8210-8214(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
[3]"Identification of novel immunodominant epididymal sperm proteins using combinatorial approach."
Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.
Reproduction 138:81-93(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
[4]"Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early endocytic vesicles."
Mukhopadhyay A., Nieves E., Che F.Y., Wang J., Jin L., Murray J.W., Gordon K., Angeletti R.H., Wolkoff A.W.
J. Cell Sci. 124:765-775(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02998 mRNA. Translation: AAA42006.1.
BC066662 mRNA. Translation: AAH66662.1.
PIRTVRTYP. A39963.
RefSeqNP_112352.2. NM_031090.2.
UniGeneRn.106743.

3D structure databases

ProteinModelPortalQ6NYB7.
SMRQ6NYB7. Positions 6-176.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid249626. 1 interaction.
IntActQ6NYB7. 1 interaction.
MINTMINT-4576596.

PTM databases

PhosphoSiteQ6NYB7.

Proteomic databases

PaxDbQ6NYB7.
PRIDEQ6NYB7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID81754.
KEGGrno:81754.
UCSCRGD:619736. rat.

Organism-specific databases

CTD5861.
RGD619736. Rab1.

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233968.
HOVERGENHBG009351.
KOK07874.
PhylomeDBQ6NYB7.

Gene expression databases

GenevestigatorQ6NYB7.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20817273.
PROQ6NYB7.

Entry information

Entry nameRAB1A_RAT
AccessionPrimary (citable) accession number: Q6NYB7
Secondary accession number(s): P05711
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families