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Protein

Ras-related protein Rab-1A

Gene

Rab1A

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in anterograde melanosome transport (By similarity). Plays a role in microtubule-dependent protein transport by early endosomes.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269GTPBy similarity
Nucleotide bindingi36 – 438GTPBy similarity
Nucleotide bindingi66 – 705GTPBy similarity
Nucleotide bindingi124 – 1274GTPBy similarity
Nucleotide bindingi154 – 1563GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: RGD

GO - Biological processi

  • autophagic vacuole assembly Source: UniProtKB
  • autophagy Source: UniProtKB
  • cell migration Source: UniProtKB
  • defense response to bacterium Source: UniProtKB
  • endocytosis Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: MGI
  • growth hormone secretion Source: UniProtKB
  • interleukin-8 secretion Source: UniProtKB
  • intracellular protein transport Source: GO_Central
  • Rab protein signal transduction Source: GO_Central
  • vesicle transport along microtubule Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Autophagy, ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-1A
Gene namesi
Name:Rab1A
Synonyms:Rab1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619736. Rab1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • early endosome Source: UniProtKB
  • endoplasmic reticulum membrane Source: RGD
  • Golgi membrane Source: RGD
  • melanosome Source: UniProtKB-SubCell
  • neuronal cell body Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 205204Ras-related protein Rab-1APRO_0000121059Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei79 – 791O-(2-cholinephosphoryl)serineBy similarity
Modified residuei194 – 1941Phosphoserine; by CDK1By similarity
Lipidationi204 – 2041S-geranylgeranyl cysteineBy similarity
Lipidationi205 – 2051S-geranylgeranyl cysteineBy similarity

Post-translational modificationi

Phosphorylated by CDK1 kinase during mitosis.By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ6NYB7.
PRIDEiQ6NYB7.

PTM databases

PhosphoSiteiQ6NYB7.

Expressioni

Tissue specificityi

Expressed in flagella of epididymal sperm.1 Publication

Gene expression databases

GenevisibleiQ6NYB7. RN.

Interactioni

Subunit structurei

May interact with YIPF5. Interacts with GDI1; this promotes dissociation from membranes. Interacts with C9orf72 (By similarity).By similarity

Protein-protein interaction databases

BioGridi249626. 2 interactions.
IntActiQ6NYB7. 1 interaction.
MINTiMINT-4576596.
STRINGi10116.ENSRNOP00000067788.

Structurei

3D structure databases

ProteinModelPortaliQ6NYB7.
SMRiQ6NYB7. Positions 6-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 489Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ6NYB7.
KOiK07874.
PhylomeDBiQ6NYB7.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6NYB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSMNPEYDY LFKLLLIGDS GVGKSCLLLR FADDTYTESY ISTIGVDFKI
60 70 80 90 100
RTIELDGKTI KLQIWDTAGQ ERFRTITSSY YRGAHGIIVV YDVTDQESFN
110 120 130 140 150
NVKQWLQEID RYASENVNKL LVGNKCDLTT KKVVDYTTAK EFADSLGIPF
160 170 180 190 200
LETSAKNATN VEQSFMTMAA EIKKRMGPGA TAGGAEKSNV KIQSTPVKQS

GGGCC
Length:205
Mass (Da):22,678
Last modified:January 23, 2007 - v3
Checksum:iB2A8F4E3B0FB17D6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1592AT → EK in AAA42006 (PubMed:3317403).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02998 mRNA. Translation: AAA42006.1.
BC066662 mRNA. Translation: AAH66662.1.
PIRiA39963. TVRTYP.
RefSeqiNP_112352.2. NM_031090.2.
UniGeneiRn.106743.

Genome annotation databases

GeneIDi81754.
KEGGirno:81754.
UCSCiRGD:619736. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02998 mRNA. Translation: AAA42006.1.
BC066662 mRNA. Translation: AAH66662.1.
PIRiA39963. TVRTYP.
RefSeqiNP_112352.2. NM_031090.2.
UniGeneiRn.106743.

3D structure databases

ProteinModelPortaliQ6NYB7.
SMRiQ6NYB7. Positions 6-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249626. 2 interactions.
IntActiQ6NYB7. 1 interaction.
MINTiMINT-4576596.
STRINGi10116.ENSRNOP00000067788.

PTM databases

PhosphoSiteiQ6NYB7.

Proteomic databases

PaxDbiQ6NYB7.
PRIDEiQ6NYB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81754.
KEGGirno:81754.
UCSCiRGD:619736. rat.

Organism-specific databases

CTDi5861.
RGDi619736. Rab1.

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ6NYB7.
KOiK07874.
PhylomeDBiQ6NYB7.

Miscellaneous databases

NextBioi615518.
PROiQ6NYB7.

Gene expression databases

GenevisibleiQ6NYB7. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Four additional members of the ras gene superfamily isolated by an oligonucleotide strategy: molecular cloning of YPT-related cDNAs from a rat brain library."
    Touchot N., Chardin P., Tavitian A.
    Proc. Natl. Acad. Sci. U.S.A. 84:8210-8214(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. "Identification of novel immunodominant epididymal sperm proteins using combinatorial approach."
    Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.
    Reproduction 138:81-93(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
  4. "Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early endocytic vesicles."
    Mukhopadhyay A., Nieves E., Che F.Y., Wang J., Jin L., Murray J.W., Gordon K., Angeletti R.H., Wolkoff A.W.
    J. Cell Sci. 124:765-775(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRAB1A_RAT
AccessioniPrimary (citable) accession number: Q6NYB7
Secondary accession number(s): P05711
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.