ID   KANK3_HUMAN             Reviewed;         821 AA.
AC   Q6NY19; Q6NZI1; Q6ZQR3; Q8IUV2;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 2.
DT   24-JAN-2024, entry version 152.
DE   RecName: Full=KN motif and ankyrin repeat domain-containing protein 3 {ECO:0000305};
DE   AltName: Full=Ankyrin repeat domain-containing protein 47;
GN   Name=KANK3 {ECO:0000312|HGNC:HGNC:24796}; Synonyms=ANKRD47;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANTS TYR-288
RP   AND THR-485.
RC   TISSUE=Mammary gland;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain, and Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   POSSIBLE FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=17996375; DOI=10.1016/j.bbagen.2007.09.017;
RA   Zhu Y., Kakinuma N., Wang Y., Kiyama R.;
RT   "Kank proteins: a new family of ankyrin-repeat domain-containing
RT   proteins.";
RL   Biochim. Biophys. Acta 1780:128-133(2008).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152; SER-167; SER-168;
RP   SER-177; SER-271 AND SER-293, AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- FUNCTION: May be involved in the control of cytoskeleton formation by
CC       regulating actin polymerization.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=2;
CC         IsoId=Q6NY19-2; Sequence=Displayed;
CC       Name=1;
CC         IsoId=Q6NY19-1; Sequence=VSP_061119;
CC   -!- TISSUE SPECIFICITY: Strongly expressed in breast, liver, lung, skeletal
CC       muscle and kidney. {ECO:0000269|PubMed:17996375}.
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DR   EMBL; AK128815; BAC87620.1; -; mRNA.
DR   EMBL; BC035849; AAH35849.1; -; mRNA.
DR   EMBL; BC066124; AAH66124.1; -; mRNA.
DR   EMBL; BC066775; AAH66775.1; -; mRNA.
DR   CCDS; CCDS12199.1; -. [Q6NY19-2]
DR   RefSeq; NP_940873.2; NM_198471.2. [Q6NY19-2]
DR   RefSeq; XP_006722781.1; XM_006722718.3. [Q6NY19-2]
DR   RefSeq; XP_011526186.1; XM_011527884.2. [Q6NY19-2]
DR   AlphaFoldDB; Q6NY19; -.
DR   SMR; Q6NY19; -.
DR   BioGRID; 129185; 7.
DR   IntAct; Q6NY19; 1.
DR   STRING; 9606.ENSP00000328923; -.
DR   iPTMnet; Q6NY19; -.
DR   PhosphoSitePlus; Q6NY19; -.
DR   BioMuta; KANK3; -.
DR   DMDM; 74749039; -.
DR   EPD; Q6NY19; -.
DR   jPOST; Q6NY19; -.
DR   MassIVE; Q6NY19; -.
DR   PaxDb; 9606-ENSP00000328923; -.
DR   PeptideAtlas; Q6NY19; -.
DR   ProteomicsDB; 66779; -. [Q6NY19-1]
DR   ProteomicsDB; 66780; -. [Q6NY19-2]
DR   Antibodypedia; 42728; 76 antibodies from 18 providers.
DR   DNASU; 256949; -.
DR   Ensembl; ENST00000330915.7; ENSP00000328923.2; ENSG00000186994.12. [Q6NY19-2]
DR   Ensembl; ENST00000593649.5; ENSP00000470728.1; ENSG00000186994.12. [Q6NY19-1]
DR   GeneID; 256949; -.
DR   KEGG; hsa:256949; -.
DR   MANE-Select; ENST00000330915.7; ENSP00000328923.2; NM_198471.3; NP_940873.2.
DR   UCSC; uc010dwa.3; human. [Q6NY19-2]
DR   AGR; HGNC:24796; -.
DR   CTD; 256949; -.
DR   DisGeNET; 256949; -.
DR   GeneCards; KANK3; -.
DR   HGNC; HGNC:24796; KANK3.
DR   HPA; ENSG00000186994; Low tissue specificity.
DR   MIM; 614611; gene.
DR   neXtProt; NX_Q6NY19; -.
DR   OpenTargets; ENSG00000186994; -.
DR   PharmGKB; PA162392612; -.
DR   VEuPathDB; HostDB:ENSG00000186994; -.
DR   eggNOG; KOG0514; Eukaryota.
DR   GeneTree; ENSGT00940000161178; -.
DR   HOGENOM; CLU_004269_2_0_1; -.
DR   InParanoid; Q6NY19; -.
DR   OMA; SDAWVTE; -.
DR   OrthoDB; 123304at2759; -.
DR   PhylomeDB; Q6NY19; -.
DR   TreeFam; TF324499; -.
DR   PathwayCommons; Q6NY19; -.
DR   SignaLink; Q6NY19; -.
DR   BioGRID-ORCS; 256949; 15 hits in 1147 CRISPR screens.
DR   ChiTaRS; KANK3; human.
DR   GenomeRNAi; 256949; -.
DR   Pharos; Q6NY19; Tdark.
DR   PRO; PR:Q6NY19; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q6NY19; Protein.
DR   Bgee; ENSG00000186994; Expressed in vena cava and 167 other cell types or tissues.
DR   ExpressionAtlas; Q6NY19; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005856; C:cytoskeleton; IBA:GO_Central.
DR   GO; GO:0030837; P:negative regulation of actin filament polymerization; IBA:GO_Central.
DR   Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR   InterPro; IPR002110; Ankyrin_rpt.
DR   InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR   InterPro; IPR047184; KANK1-4.
DR   InterPro; IPR021939; KN_motif.
DR   PANTHER; PTHR24168; KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING; 1.
DR   PANTHER; PTHR24168:SF23; KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 3; 1.
DR   Pfam; PF12796; Ank_2; 2.
DR   Pfam; PF12075; KN_motif; 1.
DR   PRINTS; PR01415; ANKYRIN.
DR   SMART; SM00248; ANK; 4.
DR   SUPFAM; SSF48403; Ankyrin repeat; 1.
DR   PROSITE; PS50297; ANK_REP_REGION; 1.
DR   PROSITE; PS50088; ANK_REPEAT; 2.
DR   Genevisible; Q6NY19; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; ANK repeat; Coiled coil; Phosphoprotein;
KW   Reference proteome; Repeat.
FT   CHAIN           1..821
FT                   /note="KN motif and ankyrin repeat domain-containing
FT                   protein 3"
FT                   /id="PRO_0000244582"
FT   REPEAT          622..652
FT                   /note="ANK 1"
FT   REPEAT          656..690
FT                   /note="ANK 2"
FT   REPEAT          695..724
FT                   /note="ANK 3"
FT   REPEAT          728..758
FT                   /note="ANK 4"
FT   REPEAT          762..785
FT                   /note="ANK 5"
FT   REGION          1..36
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          58..184
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          224..333
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          385..547
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          784..821
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          181..230
FT                   /evidence="ECO:0000255"
FT   COILED          367..404
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        128..146
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        224..266
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        397..411
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        418..432
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        459..481
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        497..517
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        787..808
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         152
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   MOD_RES         160
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Z1P7"
FT   MOD_RES         164
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Z1P7"
FT   MOD_RES         167
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   MOD_RES         168
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   MOD_RES         177
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   MOD_RES         271
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   MOD_RES         280
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Z1P7"
FT   MOD_RES         293
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   VAR_SEQ         795..821
FT                   /note="SESPPGSQTATPGEGECGDNGENPQVQ -> AGVQRHNLSSLQPPPPRFKKF
FT                   SCLSLPSSWDYNSCEPSRLAQLTIF (in isoform 1)"
FT                   /id="VSP_061119"
FT   VARIANT         288
FT                   /note="D -> Y (in dbSNP:rs890850)"
FT                   /evidence="ECO:0000269|PubMed:14702039"
FT                   /id="VAR_026909"
FT   VARIANT         359
FT                   /note="R -> H (in dbSNP:rs890853)"
FT                   /id="VAR_026910"
FT   VARIANT         485
FT                   /note="A -> T (in dbSNP:rs2913955)"
FT                   /evidence="ECO:0000269|PubMed:14702039"
FT                   /id="VAR_026911"
SQ   SEQUENCE   821 AA;  85893 MW;  66CCB7F5B1B251F5 CRC64;
     MAKFALNQNL PDLGGPRLCP VPAAGGARSP SSPYSVETPY GFHLDLDFLK YIEELERGPA
     ARRAPGPPTS RRPRAPRPGL AGARSPGAWT SSESLASDDG GAPGILSQGA PSGLLMQPLS
     PRAPVRNPRV EHTLRETSRR LELAQTHERA PSPGRGVPRS PRGSGRSSPA PNLAPASPGP
     AQLQLVREQM AAALRRLREL EDQARTLPEL QEQVRALRAE KARLLAGRAQ PEPDGEAETR
     PDKLAQLRRL TERLATSERG GRARASPRAD SPDGLAAGRS EGALQVLDGE VGSLDGTPQT
     REVAAEAVPE TREAGAQAVP ETREAGVEAA PETVEADAWV TEALLGLPAA AERELELLRA
     SLEHQRGVSE LLRGRLRELE EAREAAEEAA AGARAQLREA TTQTPWSCAE KAAQTESPAE
     APSLTQESSP GSMDGDRAVA PAGILKSIMK KRDGTPGAQP SSGPKSLQFV GVLNGEYESS
     SSEDASDSDG DSENGGAEPP GSSSGSGDDS GGGSDSGTPG PPSGGDIRDP EPEAEAEPQQ
     VAQGRCELSP RLREACVALQ RQLSRPRGVA SDGGAVRLVA QEWFRVSSQR RSQAEPVARM
     LEGVRRLGPE LLAHVVNLAD GNGNTALHYS VSHGNLAIAS LLLDTGACEV NRQNRAGYSA
     LMLAALTSVR QEEEDMAVVQ RLFCMGDVNA KASQTGQTAL MLAISHGRQD MVATLLACGA
     DVNAQDADGA TALMCASEYG RLDTVRLLLT QPGCDPAILD NEGTSALAIA LEAEQDEVAA
     LLHAHLSSGQ PDTQSESPPG SQTATPGEGE CGDNGENPQV Q
//