ID   Q6NXI4_MOUSE            Unreviewed;      1511 AA.
AC   Q6NXI4;
DT   05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2004, sequence version 1.
DT   27-MAR-2024, entry version 146.
DE   RecName: Full=Rap guanine nucleotide exchange factor 2 {ECO:0000256|ARBA:ARBA00016709};
DE   AltName: Full=Cyclic nucleotide ras GEF {ECO:0000256|ARBA:ARBA00031545};
DE   AltName: Full=Neural RAP guanine nucleotide exchange protein {ECO:0000256|ARBA:ARBA00032021};
DE   AltName: Full=PDZ domain-containing guanine nucleotide exchange factor 1 {ECO:0000256|ARBA:ARBA00030673};
DE   AltName: Full=RA-GEF-1 {ECO:0000256|ARBA:ARBA00029925};
DE   AltName: Full=Ras/Rap1-associating GEF-1 {ECO:0000256|ARBA:ARBA00031980};
DE   Flags: Fragment;
GN   Name=Rapgef2 {ECO:0000313|EMBL:AAH67056.1,
GN   ECO:0000313|MGI:MGI:2659071};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|EMBL:AAH67056.1};
RN   [1] {ECO:0000313|EMBL:AAH67056.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6 {ECO:0000313|EMBL:AAH67056.1};
RC   TISSUE=Brain {ECO:0000313|EMBL:AAH67056.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RA   Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H.,
RA   Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M.,
RA   Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.,
RA   Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F.,
RA   Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T.,
RA   Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F.,
RA   Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E.,
RA   Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y.,
RA   Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E.,
RA   Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y.,
RA   Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W.,
RA   Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J.,
RA   Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W.,
RA   Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J.,
RA   Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I.,
RA   Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U.,
RA   Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A.,
RA   Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBCELLULAR LOCATION: Cell junction {ECO:0000256|ARBA:ARBA00004282}.
CC       Cell membrane {ECO:0000256|ARBA:ARBA00004236}. Cytoplasm, perinuclear
CC       region {ECO:0000256|ARBA:ARBA00004556}. Endosome
CC       {ECO:0000256|ARBA:ARBA00004177}. Late endosome
CC       {ECO:0000256|ARBA:ARBA00004603}.
CC   -!- SIMILARITY: Belongs to the RAPGEF2 family.
CC       {ECO:0000256|ARBA:ARBA00010829}.
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DR   EMBL; BC067056; AAH67056.1; -; mRNA.
DR   RefSeq; NP_001093094.2; NM_001099624.3.
DR   RefSeq; NP_001297465.1; NM_001310536.1.
DR   IntAct; Q6NXI4; 1.
DR   PeptideAtlas; Q6NXI4; -.
DR   GeneID; 76089; -.
DR   KEGG; mmu:76089; -.
DR   AGR; MGI:2659071; -.
DR   CTD; 9693; -.
DR   MGI; MGI:2659071; Rapgef2.
DR   OrthoDB; 5473909at2759; -.
DR   BioGRID-ORCS; 76089; 2 hits in 61 CRISPR screens.
DR   ChiTaRS; Rapgef2; mouse.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR   GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR   GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR   CDD; cd00038; CAP_ED; 1.
DR   CDD; cd00992; PDZ_signaling; 1.
DR   CDD; cd01785; RA_PDZ-GEF1; 1.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 2.30.42.10; -; 1.
DR   Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR   Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR   Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR018490; cNMP-bd_dom_sf.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR000159; RA_dom.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR45161; CYTOSKELETON-ASSOCIATED PROTEIN 4; 1.
DR   PANTHER; PTHR45161:SF2; RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2; 1.
DR   Pfam; PF00595; PDZ; 1.
DR   Pfam; PF00788; RA; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00100; cNMP; 1.
DR   SMART; SM00228; PDZ; 1.
DR   SMART; SM00314; RA; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF51206; cAMP-binding domain-like; 1.
DR   SUPFAM; SSF50156; PDZ domain-like; 1.
DR   SUPFAM; SSF48366; Ras GEF; 1.
DR   SUPFAM; SSF54236; Ubiquitin-like; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
DR   PROSITE; PS50106; PDZ; 1.
DR   PROSITE; PS50200; RA; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
DR   Genevisible; Q6NXI4; MM.
PE   2: Evidence at transcript level;
KW   Cell junction {ECO:0000256|ARBA:ARBA00022949};
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Differentiation {ECO:0000256|ARBA:ARBA00022782};
KW   Endosome {ECO:0000256|ARBA:ARBA00022753};
KW   Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW   ECO:0000256|PROSITE-ProRule:PRU00168};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Neurogenesis {ECO:0000256|ARBA:ARBA00022902};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}.
FT   DOMAIN          150..250
FT                   /note="Cyclic nucleotide-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS50042"
FT   DOMAIN          282..395
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000259|PROSITE:PS50212"
FT   DOMAIN          400..470
FT                   /note="PDZ"
FT                   /evidence="ECO:0000259|PROSITE:PS50106"
FT   DOMAIN          621..707
FT                   /note="Ras-associating"
FT                   /evidence="ECO:0000259|PROSITE:PS50200"
FT   DOMAIN          732..959
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000259|PROSITE:PS50009"
FT   REGION          85..114
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1017..1066
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1108..1174
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1239..1271
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1335..1384
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1405..1511
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        96..111
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1040..1064
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1120..1143
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1150..1174
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1335..1350
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1353..1367
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:AAH67056.1"
SQ   SEQUENCE   1511 AA;  168517 MW;  FC65F1077673A757 CRC64;
     LRGIFFYLEK EAFSTQEMAF LVRCYANCLQ PWSSKLPADF TKLHLTDSLH PQVTHVSSSH
     SGCSITSDSG SSSLSDIYQA TESEAGDMDL SGLPETAVDS EDDDDEEDIE RASDPLMSRD
     IVRDCLEKDP IDRTDDDIEQ LLEFMHQLPA FANMTMSVRR ELCAVMVFAV VERAGTIVLN
     DGEELDSWSV ILNGSVEVTY PDGKAEILCM GNSFGVSPTM DKEYMKGVMR TKVDDCQFVC
     IAQQDYCRIL NQVEKNMQKV EEEGEIVMVK EHRELDRTGT RKGHIVIKGT SERLTMHLVE
     EHSVVDPTFI EDFLLTYRTF LSSPMEVGKK LLEWFNDPSL RDKVTRVVLL WVNNHFNDFE
     GDPAMTRFLE EFENNLEREK MGGHLRLLNI ACAAKAKRRL MTLTKPSREA PLPFILLGGS
     EKGFGIFVDS VDSCSKATEA GLKRGDQILE VNGQNFENIQ LSKAMEILRN NTHLSITVKT
     NLFVFKELLT RLSEEKRNGA PHLPKIGDIK KASRYSIPDL AVDVEQVIGL EKVNKKSKAN
     TVGGRNKLKK ILDKTRISIL PQKPYNDIGI GQSQDDSIVG LRQTKHIPAA LPVSGTLSSS
     NPDLLQSHHR ILDFSTTPDL PDQVLRVFKA DQQSRYIMIS KDTTAKEVVI QAIREFAVTA
     TPEQYSLCEV SVTPEGVIKQ RRLPDQLSKL ADRIQLSGRY YLKNNMETET LCSDEDAQEL
     LRESQISLLQ LSTVEVATQL SMRNFELFRN IEPTEYIDDL FKLKSKTSCA NLKKFEEVIN
     QETFWVASEI LRETNQLKRM KIIKHFIKIA LHCRECKNFN SMFAIISGLN LAPVARLRTT
     WEKLPNKYEK LFQDLQDLFD PSRNMAKYRN VLSGQNLQPP VIPLFPVIKK DLTFLHEGND
     SKVDGLVNFE KLRMIAKEIR HVGRMASVNM DPALMFRTRK KKWRSLGSLS QGSANATVLD
     VAQTGGHKKR VRRSSFLNAK KLYEDAQMAR KVKQYLSNLE LEMDEESLQT LSLQCEPATS
     TLPKNPGDKK PVKSETSPVA PRAGPQQKVQ PQQPLAQPQP PHKVSQGLQV PAVSLYPSRK
     KVPVKDLPPF GINSPQALKK ILSLSEEGSL ERHRKQAEDT ISNASSQLSS PPTSPQSSPR
     KGYALALSGT VDNFSDSGHS EISSRSSIVS NSSFDSVPVS LHDERRQRHS VSIVESNLGV
     GRMERRTLME PDQYSLGSYA PVSESRGLYA AATVISSPST EELSHDQGDR ASLDAADSGR
     GSWTSCSSGS HDNIQTIQHQ RSWETLPFGH THFDYSGDAA SIWASGGHMD QMMFSDHSTK
     YNRQNQSRES LEQAQSRASW ASSTGYWGED SEGDTGTIKR RGGKDVSAEA ESSSMVPVTT
     EEAKPVPMPA HIAVTPSTTK GLIARKEGRY REPPPTPPGY VGIPIADFPE GPCHPARKPP
     DYNVALQRSR MVARPTEAPA PGRTPPAAAA SRPGSKPQWH KPSDADPRLA PFQPQGFAGA
     EEDEDEQVSA V
//