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Protein

Glycoprotein endo-alpha-1,2-mannosidase

Gene

Manea

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of the terminal alpha-D-glucosyl-(1,3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of the glycoprotein produced in the Golgi membrane.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-964739. N-glycan trimming and elongation in the cis-Golgi.

Protein family/group databases

CAZyiGH99. Glycoside Hydrolase Family 99.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein endo-alpha-1,2-mannosidase (EC:3.2.1.130)
Short name:
Endo-alpha mannosidase
Short name:
Endomannosidase
Short name:
mEndo
Gene namesi
Name:Manea
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2444484. Manea.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini31 – 462LumenalSequence analysisAdd BLAST432

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002823161 – 462Glycoprotein endo-alpha-1,2-mannosidaseAdd BLAST462

Post-translational modificationi

Undergoes proteolytic cleavage in the C-terminal region.By similarity

Proteomic databases

EPDiQ6NXH2.
MaxQBiQ6NXH2.
PaxDbiQ6NXH2.
PeptideAtlasiQ6NXH2.
PRIDEiQ6NXH2.

PTM databases

PhosphoSitePlusiQ6NXH2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040520.
CleanExiMM_MANEA.
ExpressionAtlasiQ6NXH2. baseline and differential.
GenevisibleiQ6NXH2. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038671.

Structurei

3D structure databases

ProteinModelPortaliQ6NXH2.
SMRiQ6NXH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 462CatalyticBy similarityAdd BLAST403

Sequence similaritiesi

Belongs to the glycosyl hydrolase 99 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG22. Eukaryota.
ENOG410XT8Q. LUCA.
GeneTreeiENSGT00390000016054.
HOGENOMiHOG000223388.
HOVERGENiHBG103563.
InParanoidiQ6NXH2.
KOiK15538.
OMAiMLRPNKA.
OrthoDBiEOG091G0CUF.
PhylomeDBiQ6NXH2.
TreeFamiTF324051.

Family and domain databases

CDDicd11574. GH99. 1 hit.
InterProiIPR026071. Glyco_Hydrolase_99.
[Graphical view]
PANTHERiPTHR13572. PTHR13572. 1 hit.
PfamiPF16317. Glyco_hydro_99. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NXH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFRRRTCI LLSLFILFIF SLMMGLKMLW PNAASFGPPF GLDLLPELHP
60 70 80 90 100
LNAHSGNKAD FQRSDRINME TNTKALKGAG MTVLPAKASE VNLEELPPLN
110 120 130 140 150
YFLHAFYYSW YGNPQFDGKY IHWNHPVLEH WDPRIAKNYP QGQHSPPDDI
160 170 180 190 200
GSSFYPELGS YSSRDPSVIE THMKQMRSAS IGVLALSWYP PDSRDDNGEA
210 220 230 240 250
TDHLVPTILD KAHKYNLKVT FHIEPYSNRD DQNMHQNIKY IIDKYGNHPA
260 270 280 290 300
FYRYKTRTGH SLPMFYVYDS YITKPTIWAN LLTPSGSQSV RSSPYDGLFI
310 320 330 340 350
ALLVEEKHKN DILQSGFDGI YTYFATNGFT YGSSHQNWNN LKSFCEKNNL
360 370 380 390 400
MFIPSVGPGY IDTSIRPWNT QNTRNRVNGK YYEVGLSAAL QTHPSLISIT
410 420 430 440 450
SFNEWHEGTQ IEKAVPKRTA NTIYLDYRPH KPSLYLELTR KWSEKFSKER
460
MTYALDQQQP AS
Length:462
Mass (Da):53,183
Last modified:July 5, 2004 - v1
Checksum:i01262CFE7DD9FC5F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti294P → L in BAC26805 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030141 mRNA. Translation: BAC26805.1.
AL805949 Genomic DNA. Translation: CAM23963.1.
BC067076 mRNA. Translation: AAH67076.1.
CCDSiCCDS18012.1.
RefSeqiNP_766453.2. NM_172865.2.
XP_006537962.1. XM_006537899.3.
UniGeneiMm.245602.

Genome annotation databases

EnsembliENSMUST00000041374; ENSMUSP00000038671; ENSMUSG00000040520.
GeneIDi242362.
KEGGimmu:242362.
UCSCiuc008sej.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030141 mRNA. Translation: BAC26805.1.
AL805949 Genomic DNA. Translation: CAM23963.1.
BC067076 mRNA. Translation: AAH67076.1.
CCDSiCCDS18012.1.
RefSeqiNP_766453.2. NM_172865.2.
XP_006537962.1. XM_006537899.3.
UniGeneiMm.245602.

3D structure databases

ProteinModelPortaliQ6NXH2.
SMRiQ6NXH2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038671.

Protein family/group databases

CAZyiGH99. Glycoside Hydrolase Family 99.

PTM databases

PhosphoSitePlusiQ6NXH2.

Proteomic databases

EPDiQ6NXH2.
MaxQBiQ6NXH2.
PaxDbiQ6NXH2.
PeptideAtlasiQ6NXH2.
PRIDEiQ6NXH2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041374; ENSMUSP00000038671; ENSMUSG00000040520.
GeneIDi242362.
KEGGimmu:242362.
UCSCiuc008sej.1. mouse.

Organism-specific databases

CTDi79694.
MGIiMGI:2444484. Manea.

Phylogenomic databases

eggNOGiENOG410IG22. Eukaryota.
ENOG410XT8Q. LUCA.
GeneTreeiENSGT00390000016054.
HOGENOMiHOG000223388.
HOVERGENiHBG103563.
InParanoidiQ6NXH2.
KOiK15538.
OMAiMLRPNKA.
OrthoDBiEOG091G0CUF.
PhylomeDBiQ6NXH2.
TreeFamiTF324051.

Enzyme and pathway databases

ReactomeiR-MMU-964739. N-glycan trimming and elongation in the cis-Golgi.

Miscellaneous databases

PROiQ6NXH2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040520.
CleanExiMM_MANEA.
ExpressionAtlasiQ6NXH2. baseline and differential.
GenevisibleiQ6NXH2. MM.

Family and domain databases

CDDicd11574. GH99. 1 hit.
InterProiIPR026071. Glyco_Hydrolase_99.
[Graphical view]
PANTHERiPTHR13572. PTHR13572. 1 hit.
PfamiPF16317. Glyco_hydro_99. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMANEA_MOUSE
AccessioniPrimary (citable) accession number: Q6NXH2
Secondary accession number(s): Q8C0N9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.