Reviewed, UniProtKB/Swiss-Prot Q6NWG4 (ANM6_DANRE)

Names and origin

Protein names

Recommended name:
    Protein arginine N-methyltransferase 6
    EC=2.1.1.-
Alternative name(s):
    Histone-arginine N-methyltransferase PRMT6
    EC=2.1.1.125

Gene names

Name:

prmt6

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as hoxa2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (polb), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates hmga1 By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone-arginine = S-adenosyl-L-homocysteine + histone-N(omega)-methyl-arginine.
Subcellular location	Nucleus By similarity.
Post-translational modification	Automethylated By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family. PRMT6 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
PTM	Methylation
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW histone H3-R2 methylation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB peptidyl-arginine methylation, to asymmetrical-dimethyl arginine Inferred from sequence or structural similarity. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	histone binding Inferred from sequence or structural similarity. Source: UniProtKB histone methyltransferase activity (H2A-R3 specific) Inferred from sequence or structural similarity. Source: UniProtKB histone methyltransferase activity (H3-R2 specific) Inferred from sequence or structural similarity. Source: UniProtKB histone methyltransferase activity (H4-R3 specific) Inferred from sequence or structural similarity. Source: UniProtKB protein-arginine omega-N asymmetric methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB protein-arginine omega-N monomethyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Protein arginine N-methyltransferase 6		PRO_0000378150
Sites
	Binding site	30	1	S-adenosyl-L-methionine By similarity
	Binding site	39	1	S-adenosyl-L-methionine By similarity
	Binding site	63	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	85	1	S-adenosyl-L-methionine By similarity
	Binding site	114	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NWG4-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: F5333FFA35EBA893	FASTA	349	39,251
	10 20 30 40 50 60 MSQHATKKRK LDRSTEDYMY FDSYSDVTIH EEMIADTVRT NTYRMGIFKN SKSIEGKVVL 70 80 90 100 110 120 DVGAGTGVLS LFCAQAGARK VYAVEASSIA DQAVKIVKLN QMEDRIEVIK STLETIELAE 130 140 150 160 170 180 KVDVIVSEWM GYALLHESML NSVIFARDKW LKPGGLILPS RADLYIAPIN DVVVEGRLDF 190 200 210 220 230 240 WSTVKGQYGV DMSCMTDFAR KCIMNKDITV NPVTVEDVLS HPCKFAELDL NTVTLEQLRD 250 260 270 280 290 300 VNGSFSCVCF GSSSIHAFCV WFTVTFPAEE KALVLSTSPF KAETHWKQAV LYLDDAVDVM 310 320 330 340 QDTKVEGEIS LYPSEENSRH ICIRVDYVIG EQKKHSKSFS IPDQYLEVK

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC058308 mRNA. Translation: AAH58308.1. BC067600 mRNA. Translation: AAH67600.1. Different initiation.
IPI	IPI00513082.
UniGene	Dr.84227
3D structure databases
ModBase	Search...
Organism-specific databases
ZFIN	ZDB-GENE-040914-7. prmt6.
Phylogenomic databases
HOVERGEN	Q6NWG4.
Gene expression databases
ArrayExpress	Q6NWG4.
Family and domain databases
InterPro	IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANM6_DANRE

Accession

Primary (citable) accession number: Q6NWG4
Secondary accession number(s): Q6PE31

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	September 22, 2009
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project