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Protein

Mitochondrial import inner membrane translocase subunit TIM50

Gene

timm50

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.1 Publication

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: ZFIN
  • mitochondrial membrane organization Source: UniProtKB
  • protein dephosphorylation Source: UniProtKB
  • protein import into mitochondrial matrix Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM50
Gene namesi
Name:timm50
Synonyms:tim50
ORF Names:zgc:66357
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-040426-1618. timm50.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 10074Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Topological domaini122 – 387266Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Massive apoptosis in the CNS, probably due to loss of mitochondrial function and increased cytochrome c release.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626MitochondrionSequence analysisAdd
BLAST
Chaini27 – 387361Mitochondrial import inner membrane translocase subunit TIM50PRO_0000043118Add
BLAST

Proteomic databases

PaxDbiQ6NWD4.

Expressioni

Developmental stagei

At 48 hours post-fertilization (hpf), it is highly expressed in the CNS, particularly in the brain tissues. The pattern is the same at 72 hpf.1 Publication

Interactioni

Subunit structurei

Component of the TIM23 complex at least composed of timm23, timm17 and timm50.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000048422.

Structurei

3D structure databases

ProteinModelPortaliQ6NWD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini178 – 321144FCP1 homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TIM50 family.Curated
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2832. Eukaryota.
COG5190. LUCA.
HOGENOMiHOG000044824.
HOVERGENiHBG084157.
InParanoidiQ6NWD4.
KOiK17496.
PhylomeDBiQ6NWD4.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
IPR027111. Tim50.
[Graphical view]
PANTHERiPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6NWD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVSVYPMC VRASRGLLRL RQGARCSTAP PLLDVVRPLS ADTSSSSATG
60 70 80 90 100
GLAQAILQER LQQQQKSQEQ PPPEGEDSGH KQDEQGEDKK QKENTAYAKK
110 120 130 140 150
MVLRLAGIMG LGGTVGIVYI FGSNSVDEQG NKIPDEFDND VPVIQQLRRT
160 170 180 190 200
FKYFKDYRQM IIEPTSPKLL PDPLREPYYQ PPYTLVLELT DVLLHPEWSL
210 220 230 240 250
ATGWRFKKRP GIDYLFQQLA PLYEIVIFTS ETGMTAYPLI DSIDPQGFVM
260 270 280 290 300
YRLFRDATRY MEGHHVKDVS CLNRDTSKVI VVDCKREAFG LQPFNGLALC
310 320 330 340 350
KWDGNSEDRT LYDLAAFLKT IATSGVEDVR SVLENYAHEE DPIEAFKRRQ
360 370 380
AQLAREEEQR ISEMAQQKKQ GFSLGTIAGR FWSRKQQ
Length:387
Mass (Da):43,881
Last modified:December 6, 2005 - v2
Checksum:i00F49E27F1E1C9D9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti323 – 3231T → I in AAH67634 (Ref. 2) Curated
Sequence conflicti355 – 3551R → Q in AAH67634 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY551343 mRNA. Translation: AAT01210.1.
BC057522 mRNA. Translation: AAH57522.1.
BC067634 mRNA. Translation: AAH67634.1.
RefSeqiNP_956959.1. NM_200665.2.
UniGeneiDr.5208.

Genome annotation databases

GeneIDi393638.
KEGGidre:393638.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY551343 mRNA. Translation: AAT01210.1.
BC057522 mRNA. Translation: AAH57522.1.
BC067634 mRNA. Translation: AAH67634.1.
RefSeqiNP_956959.1. NM_200665.2.
UniGeneiDr.5208.

3D structure databases

ProteinModelPortaliQ6NWD4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000048422.

Proteomic databases

PaxDbiQ6NWD4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi393638.
KEGGidre:393638.

Organism-specific databases

CTDi92609.
ZFINiZDB-GENE-040426-1618. timm50.

Phylogenomic databases

eggNOGiKOG2832. Eukaryota.
COG5190. LUCA.
HOGENOMiHOG000044824.
HOVERGENiHBG084157.
InParanoidiQ6NWD4.
KOiK17496.
PhylomeDBiQ6NWD4.

Miscellaneous databases

PROiQ6NWD4.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR004274. FCP1_dom.
IPR023214. HAD-like_dom.
IPR027111. Tim50.
[Graphical view]
PANTHERiPTHR12210:SF3. PTHR12210:SF3. 1 hit.
PfamiPF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
PROSITEiPS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death."
    Guo Y., Cheong N., Zhang Z., De Rose R., Deng Y., Farber S.A., Fernandes-Alnemri T., Alnemri E.S.
    J. Biol. Chem. 279:24813-24825(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiTIM50_DANRE
AccessioniPrimary (citable) accession number: Q6NWD4
Secondary accession number(s): Q6PFK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 6, 2016
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.