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Protein

Spastin

Gene

spast

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. Also plays a role in axon growth and the formation of axonal branches.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi335 – 342ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • anterograde axonal transport Source: UniProtKB
  • axonal transport of mitochondrion Source: UniProtKB
  • axon extension Source: ZFIN
  • axonogenesis Source: ZFIN
  • central nervous system neuron axonogenesis Source: ZFIN
  • cytokinetic process Source: UniProtKB-HAMAP
  • cytoplasmic microtubule organization Source: ZFIN
  • endosome organization Source: ZFIN
  • ER to Golgi vesicle-mediated transport Source: UniProtKB-HAMAP
  • exit from mitosis Source: UniProtKB
  • membrane fission Source: UniProtKB
  • microtubule bundle formation Source: UniProtKB
  • microtubule cytoskeleton organization Source: ZFIN
  • microtubule severing Source: UniProtKB
  • mitotic cytokinesis Source: UniProtKB
  • mitotic spindle disassembly Source: UniProtKB
  • negative regulation of apoptotic process Source: ZFIN
  • nuclear envelope reassembly Source: UniProtKB
  • positive regulation of axon extension Source: ZFIN
  • positive regulation of microtubule depolymerization Source: ZFIN
  • protein hexamerization Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
  • reactive oxygen species metabolic process Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Differentiation, Neurogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.3. 928.

Names & Taxonomyi

Protein namesi
Recommended name:
SpastinUniRule annotation (EC:3.6.4.3UniRule annotation)
Gene namesi
Name:spastUniRule annotation
Synonyms:spg4UniRule annotation
ORF Names:si:ch73-233a22.3, zgc:85952
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 1

Organism-specific databases

ZFINiZDB-GENE-040426-2331. spast.

Subcellular locationi

  • Membrane UniRule annotation; Peripheral membrane protein UniRule annotation
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome UniRule annotation
  • Cytoplasmcytoskeleton UniRule annotation
  • Cytoplasmperinuclear region UniRule annotation
  • Nucleus UniRule annotation

  • Note: Forms a intramembrane hairpin-like structure in the membrane.UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 35CytoplasmicUniRule annotationAdd BLAST35
Intramembranei36 – 52HelicalUniRule annotationAdd BLAST17
Topological domaini53 – 570CytoplasmicUniRule annotationAdd BLAST518

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane, Microtubule, Nucleus

Pathology & Biotechi

Disruption phenotypei

Branchiomotor neurons exhibit reduced axonal outgrowth and aberrant positioning of neuronal cell bodies. Outgrowth of motor axons from the spinal cord is impaired, the number and length of spinal motor neurons is reduced, and elevated levels of apoptosis are observed in the CNS. Thickened bundles of axonal microtubules are observed in the spinal cord. Longitudinal fascicles in the hindbrain are disordered. Most embryos are immotile and fail to hatch, while those that do exhibit reduced motility and swimming defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003671371 – 570SpastinAdd BLAST570

Proteomic databases

PaxDbiQ6NW58.

Expressioni

Developmental stagei

Maternally expressed. Expressed at low levels throughout the embryo up to 24 hours post-fertilization (hpf).1 Publication

Gene expression databases

BgeeiENSDARG00000024933.

Interactioni

Subunit structurei

Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000029675.

Structurei

3D structure databases

ProteinModelPortaliQ6NW58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 158MITSequence analysisAdd BLAST76

Sequence similaritiesi

Belongs to the AAA ATPase family. Spastin subfamily.UniRule annotation
Contains 1 MIT domain.Sequence analysis

Phylogenomic databases

eggNOGiKOG0740. Eukaryota.
COG0464. LUCA.
GeneTreeiENSGT00570000078874.
HOGENOMiHOG000225146.
HOVERGENiHBG108502.
InParanoidiQ6NW58.
KOiK13254.
OMAiGNPDGDR.
OrthoDBiEOG091G0Q8J.
PhylomeDBiQ6NW58.
TreeFamiTF105014.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03021. Spastin. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
IPR027417. P-loop_NTPase.
IPR017179. Spastin.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
PIRSFiPIRSF037338. Spastin. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NW58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSGHKARLR GGRACGPVSD GSARGNRLLF YTRSLSRVPE WLLRVLLLLL
60 70 80 90 100
RWLFQPIRRA MAARAKECGP DGSEETGERI RNYHKQAFEF ISVALQIDED
110 120 130 140 150
EKGDKQKAVQ WYRKGIAELE KGIQIQVTGA GEKADRARKL QDKMITNLSM
160 170 180 190 200
AEDRLKLLGN LLSQSPAESS SDDSFYSFSN GNLRPAPASG AVSKKKDTLT
210 220 230 240 250
ITNQTSLRPK NPPKSTPNAS GLNCTPSAAQ SSRTGPQNNQ KGPTVKGKNN
260 270 280 290 300
VKASTTATAS PQRKRDMKNF KNVDSKLASL ILNEIVDSGS VVRFDDIAGQ
310 320 330 340 350
DLAKQALQEI VILPALRPEL FTGLRAPARG LLLFGPPGNG KTMLAKAVAM
360 370 380 390 400
ESNATFFNIS AATLTSKYVG EGEKLVRALF AVARELQPSI IFIDEIDSLL
410 420 430 440 450
CERREGEHDA SRRLKTEFLI EFDGVQSGGD ERVLVMGATN RPQELDEAVL
460 470 480 490 500
RRFAKRIYVA LPTEETRLKL LKNLLSKHRN PLSQKELSQL ARLTDGYSGS
510 520 530 540 550
DLTSLAKDAA LGPIRELKPE QVRNMSAHEM RDIRISDFLE SLKRIKRSVS
560 570
PQTLDQYVRW NREYGDTTGV
Length:570
Mass (Da):63,086
Last modified:March 24, 2009 - v2
Checksum:i2EE116D8B18DD9AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224C → R in AAH67715 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY304504 mRNA. Translation: AAQ74774.1.
CU651563 Genomic DNA. Translation: CAX13091.1.
BC067715 mRNA. Translation: AAH67715.1.
RefSeqiNP_998080.1. NM_212915.1.
UniGeneiDr.30307.

Genome annotation databases

EnsembliENSDART00000035150; ENSDARP00000029675; ENSDARG00000024933.
GeneIDi405851.
KEGGidre:405851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY304504 mRNA. Translation: AAQ74774.1.
CU651563 Genomic DNA. Translation: CAX13091.1.
BC067715 mRNA. Translation: AAH67715.1.
RefSeqiNP_998080.1. NM_212915.1.
UniGeneiDr.30307.

3D structure databases

ProteinModelPortaliQ6NW58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000029675.

Proteomic databases

PaxDbiQ6NW58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000035150; ENSDARP00000029675; ENSDARG00000024933.
GeneIDi405851.
KEGGidre:405851.

Organism-specific databases

CTDi6683.
ZFINiZDB-GENE-040426-2331. spast.

Phylogenomic databases

eggNOGiKOG0740. Eukaryota.
COG0464. LUCA.
GeneTreeiENSGT00570000078874.
HOGENOMiHOG000225146.
HOVERGENiHBG108502.
InParanoidiQ6NW58.
KOiK13254.
OMAiGNPDGDR.
OrthoDBiEOG091G0Q8J.
PhylomeDBiQ6NW58.
TreeFamiTF105014.

Enzyme and pathway databases

BRENDAi3.6.4.3. 928.

Miscellaneous databases

PROiQ6NW58.

Gene expression databases

BgeeiENSDARG00000024933.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03021. Spastin. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
IPR027417. P-loop_NTPase.
IPR017179. Spastin.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
PIRSFiPIRSF037338. Spastin. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPAST_DANRE
AccessioniPrimary (citable) accession number: Q6NW58
Secondary accession number(s): Q6JUU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: October 5, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.