Q6NW58 (SPAST_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spastin
EC=3.6.4.3

Gene names

Name:	spast
Synonyms:	spg4
ORF Names:	zgc:85952, si:ch73-233a22.3

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis By similarity. Required for axon growth. Ref.1
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity. Nucleus By similarity.
Developmental stage	Maternally expressed. Expressed at low levels throughout the embryo up to 24 hours post-fertilization (hpf). Ref.1
Disruption phenotype	Branchiomotor neurons exhibit reduced axonal outgrowth and aberrant positioning of neuronal cell bodies. Outgrowth of motor axons from the spinal cord is impaired, the number and length of spinal motor neurons is reduced, and elevated levels of apoptosis are observed in the CNS. Thickened bundles of axonal microtubules are observed in the spinal cord. Longitudinal fascicles in the hindbrain are disordered. Most embryos are immotile and fail to hatch, while those that do exhibit reduced motility and swimming defects. Ref.1
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule Nucleus
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	axon extension involved in development Inferred from mutant phenotype. Source: ZFIN cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW central nervous system neuron axonogenesis Inferred from mutant phenotype Ref.1. Source: ZFIN cytoplasmic microtubule organization Inferred from mutant phenotype. Source: ZFIN microtubule bundle formation Inferred from sequence or structural similarity. Source: UniProtKB microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of apoptotic process Inferred from mutant phenotype Ref.1. Source: ZFIN positive regulation of axon extension Inferred from mutant phenotype Ref.1. Source: ZFIN positive regulation of microtubule depolymerization Inferred from mutant phenotype Ref.1. Source: ZFIN protein hexamerization Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alpha-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Spastin		PRO_0000367137
Regions
	Transmembrane	38 – 54	17	Helical; Potential
	Domain	83 – 158	76	MIT
	Nucleotide binding	335 – 342	8	ATP Potential
Experimental info
	Sequence conflict	224	1	C → R in AAH67715. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q6NW58 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 2EE116D8B18DD9AA	FASTA	570	63,086
	10 20 30 40 50 60 MNSGHKARLR GGRACGPVSD GSARGNRLLF YTRSLSRVPE WLLRVLLLLL RWLFQPIRRA 70 80 90 100 110 120 MAARAKECGP DGSEETGERI RNYHKQAFEF ISVALQIDED EKGDKQKAVQ WYRKGIAELE 130 140 150 160 170 180 KGIQIQVTGA GEKADRARKL QDKMITNLSM AEDRLKLLGN LLSQSPAESS SDDSFYSFSN 190 200 210 220 230 240 GNLRPAPASG AVSKKKDTLT ITNQTSLRPK NPPKSTPNAS GLNCTPSAAQ SSRTGPQNNQ 250 260 270 280 290 300 KGPTVKGKNN VKASTTATAS PQRKRDMKNF KNVDSKLASL ILNEIVDSGS VVRFDDIAGQ 310 320 330 340 350 360 DLAKQALQEI VILPALRPEL FTGLRAPARG LLLFGPPGNG KTMLAKAVAM ESNATFFNIS 370 380 390 400 410 420 AATLTSKYVG EGEKLVRALF AVARELQPSI IFIDEIDSLL CERREGEHDA SRRLKTEFLI 430 440 450 460 470 480 EFDGVQSGGD ERVLVMGATN RPQELDEAVL RRFAKRIYVA LPTEETRLKL LKNLLSKHRN 490 500 510 520 530 540 PLSQKELSQL ARLTDGYSGS DLTSLAKDAA LGPIRELKPE QVRNMSAHEM RDIRISDFLE 550 560 570 SLKRIKRSVS PQTLDQYVRW NREYGDTTGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The microtubule-severing protein spastin is essential for axon outgrowth in the zebrafish embryo." Wood J.D., Landers J.A., Bingley M., McDermott C.J., Thomas-McArthur V., Gleadall L.J., Shaw P.J., Cunliffe V.T. Hum. Mol. Genet. 15:2763-2771(2006) [PubMed: 16893913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[2]	The Danio rerio sequencing project at the Sanger Institute Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY304504 mRNA. Translation: AAQ74774.1. CU651563 Genomic DNA. Translation: CAX13091.1. BC067715 mRNA. Translation: AAH67715.1.
IPI	IPI00497843.
RefSeq	NP_998080.1. NM_212915.1.
UniGene	Dr.30307.
3D structure databases
HSSP	HSSP built from PDB template 1YPW based on UniProtKB Q01853.
ProteinModelPortal	Q6NW58.
SMR	Q6NW58. Positions 276-561.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6NW58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000035150; ENSDARP00000029675; ENSDARG00000024933.
GeneID	405851.
KEGG	dre:405851.
Organism-specific databases
CTD	6683.
ZFIN	ZDB-GENE-040426-2331. spast.
Phylogenomic databases
eggNOG	fiNOG13914.
GeneTree	ENSGT00570000078874.
HOGENOM	HBG724153.
HOVERGEN	HBG108502.
OMA	THKSTPK.
OrthoDB	EOG4NZTTF.
PhylomeDB	Q6NW58.
Gene expression databases
ArrayExpress	Q6NW58.
Bgee	Q6NW58.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. IPR017179. Spastin. [Graphical view]
KO	K13254.
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
PIRSF	PIRSF037338. Spastin. 1 hit.
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_DANRE

Accession

Primary (citable) accession number: Q6NW58
Secondary accession number(s): Q6JUU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	November 16, 2011
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program