Q6NVP8 (FUT8_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-(1,6)-fucosyltransferase
EC=2.4.1.68

Alternative name(s):
GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
Short name=alpha1-6FucT
GDP-fucose--glycoprotein fucosyltransferase
Glycoprotein 6-alpha-L-fucosyltransferase

Gene names

Name:

fut8

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans By similarity.
Catalytic activity	GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Sequence similarities	Belongs to the glycosyltransferase 23 family. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	SH3 domain SH3-binding Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation in Golgi Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW glycoprotein 6-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Alpha-(1,6)-fucosyltransferase		PRO_0000357043
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 578	548	Lumenal Potential
	Domain	505 – 566	62	SH3
	Region	368 – 369	2	Important for donor substrate binding
	Motif	302 – 308	7	SH3-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6NVP8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C161502A464C38EE	FASTA	578	66,685
	10 20 30 40 50 60 MRPWTGSWRW IMLILFAWGT LLFYIGGHLV RDNENPDHSS RELSKILAKL ERLKQQNEDL 70 80 90 100 110 120 RRMAESLRIP EGPIEQGAAA GRIRALEEQL LKAKEQIEMY KQQSSNAVSG LGKDHEILRR 130 140 150 160 170 180 AIENGAKEFW YFVQSEVKKL KHLDRNELQR HVDEIIIDMG HQQRSVMTDL YYLSQTDGAG 190 200 210 220 230 240 DWREREAKDL TDLVQRRITY LQNPKDCSKA KKLVCNINKG CGYGCQLHHV VYCFMIAYGT 250 260 270 280 290 300 QRTLILESQS WRYATGGWET VFKPVSETCT DRSGSSTGHW SGEANDKNVQ VVELPIVDSL 310 320 330 340 350 360 HPRPPYLPLG VPEDLADRLI RLHGDPAVWW VSQFVKYLIR PQPWLEKEIE ESTKKLGFKH 370 380 390 400 410 420 PVIGVHVRRT DKVGTEAAFH PIEEYMVHVE EHFQLLARRM QIDKKRVYLA TDDPTLLQEA 430 440 450 460 470 480 KAKYPQYEFI SDNSISWSAG LHNRYTENSL RGVILDIHFL SQADFLVCTF SSQVCRVAYE 490 500 510 520 530 540 IMQTLHPDAS AHFHSLDDIY YFGGQNAHNQ LAIYPHQPRN AEEIPLEPGD IIGVAGNHWD 550 560 570 GYSKGINRKL GRTGLYPSYK VKEKIETVKY PTYQEAEK

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC067957 mRNA. Translation: AAH67957.1.
RefSeq	NP_001001232.1. NM_001001232.2.
UniGene	Str.230.
3D structure databases
HSSP	HSSP built from PDB template 1ZLM based on UniProtKB Q92882.
ProteinModelPortal	Q6NVP8.
SMR	Q6NVP8. Positions 111-575.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6NVP8.
Protein family/group databases
CAZy	GT23. Glycosyltransferase Family 23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSXETT00000016641; ENSXETP00000016641; ENSXETG00000007644.
GeneID	407913.
KEGG	xtr:407913.
Organism-specific databases
CTD	2530.
Xenbase	XB-GENE-954943. fut8.
Phylogenomic databases
eggNOG	veNOG05283.
GeneTree	ENSGT00530000063737.
HOGENOM	HBG314245.
HOVERGEN	HBG028260.
InParanoid	Q6NVP8.
OMA	LILESHN.
OrthoDB	EOG4JWVD1.
Gene expression databases
Bgee	Q6NVP8.
Family and domain databases
InterPro	IPR015827. Alpha1_6FUT_euk. IPR001452. SH3_domain. [Graphical view]
KO	K00717.
PIRSF	PIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50002. SH3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT8_XENTR

Accession

Primary (citable) accession number: Q6NVP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program