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Protein

Lysophospholipid acyltransferase LPCAT4

Gene

Lpcat4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates; converts lysophosphatidylethanolamine to phosphatidylethanolamine, 1-alkenyl-lysophatidylethanolamine to 1-alkenyl-phosphatidylethanolamine, lysophosphatidylglycerol and alkyl-lysophosphatidylcholine to phosphatidylglycerol and alkyl-phosphatidylcholine, respectively. In contrast, has no lysophosphatidylinositol, glycerol-3-phosphate, diacylglycerol or lysophosphatidic acid acyltransferase activity. Prefers long chain acyl-CoAs (C16, C18) as acyl donors (By similarity). Converts lysophosphatidylcholine to phosphatidycholine.By similarity1 Publication

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine = CoA + 1,2-diacyl-sn-glycero-3-phosphoethanolamine.1 Publication
Acyl-CoA + 1-alkenylglycerophosphoethanolamine = CoA + 1-alkenyl-2-acylglycerophosphoethanolamine.1 Publication
Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine.1 Publication
Acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine = CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine.1 Publication
Acyl-CoA + 1-acyl-sn-glycero-3-phosphatidylserine = CoA + 1,2-diacyl-sn-glycero-3-phosphatidylserine.1 Publication

Pathwayi: phospholipid metabolism

This protein is involved in the pathway phospholipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway phospholipid metabolism and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.23. 3474.
ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482925. Acyl chain remodelling of PG.
R-MMU-1483166. Synthesis of PA.
R-MMU-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00085.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipid acyltransferase LPCAT4
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 7
Short name:
1-AGP acyltransferase 7
Short name:
1-AGPAT 7
1-acylglycerophosphocholine O-acyltransferase (EC:2.3.1.23)
1-acylglycerophosphoserine O-acyltransferase (EC:2.3.1.n6)
1-alkenylglycerophosphoethanolamine O-acyltransferase (EC:2.3.1.121)
1-alkylglycerophosphocholine O-acetyltransferase (EC:2.3.1.67)
Acyltransferase-like 3
Lysophosphatidylcholine acyltransferase 4
Lysophosphatidylethanolamine acyltransferase 2 (EC:2.3.1.n7)
Plasmalogen synthase
Gene namesi
Name:Lpcat4
Synonyms:Agpat7, Aytl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2138993. Lpcat4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei40 – 6223HelicalSequence analysisAdd
BLAST
Transmembranei87 – 10721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Lysophospholipid acyltransferase LPCAT4PRO_0000247055Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ6NVG1.
MaxQBiQ6NVG1.
PaxDbiQ6NVG1.
PeptideAtlasiQ6NVG1.
PRIDEiQ6NVG1.

PTM databases

iPTMnetiQ6NVG1.
PhosphoSiteiQ6NVG1.

Expressioni

Tissue specificityi

Widely expressed with much higher level in brain. Expressed in erythroleukemic cells but not in reticulocytes.2 Publications

Gene expression databases

BgeeiQ6NVG1.
GenevisibleiQ6NVG1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028554.

Structurei

3D structure databases

ProteinModelPortaliQ6NVG1.
SMRiQ6NVG1. Positions 351-399, 406-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi129 – 1346HXXXXD motif

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4666. Eukaryota.
ENOG410XSIQ. LUCA.
GeneTreeiENSGT00390000004914.
HOGENOMiHOG000234374.
InParanoidiQ6NVG1.
KOiK13512.
OMAiLRPPHTS.
OrthoDBiEOG7NCV5G.
PhylomeDBiQ6NVG1.
TreeFamiTF323244.

Family and domain databases

InterProiIPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NVG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQGSPGAWA PLDPTSGSSA SPNPFVHELH LSGLQRVKFC LLGVLLAPIR
60 70 80 90 100
VLLAFIVLFL LWPFAWLQVA GLTEEQLQEP ITGWRKTVCH NGVLGLSRLL
110 120 130 140 150
FFLLGFLRIR VRGQRASRLE APVLVAAPHS TFFDPIVLLP CDLPKVVSRA
160 170 180 190 200
ENLSVPVIGA LLRFNQAILV SRHDPASRRR VVEEVRRRAT SGGKWPQVLF
210 220 230 240 250
FPEGTCSNKK ALLKFKPGAF IAGVPVQPVL IRYPNSLDTT SWAWRGPGVL
260 270 280 290 300
KVLWLTASQP CSIVDVEFLP VYQPSLEESK DPTLYANNVQ RVMAQALGIP
310 320 330 340 350
ATECEFVGSL PVIVVGQLKV ALEPQLWELA KVLQKAGLSP GFVDMGAEPG
360 370 380 390 400
RSRMISQEAF AQQLQLSDPQ TVAGAFSYFQ QDAKGLVDFR NVALALAALD
410 420 430 440 450
GGRSLEELTR LAFELFAEEQ AEGSDRLLYK DGFSTILHLL LGSPRPAATT
460 470 480 490 500
LHAELCQPGC SQGLSLCQFQ NFSLHDPLYG KLFSAYLRPP HKPRSTSQIP
510 520
NASSPSSPTA LANGTVQAPK QKGD
Length:524
Mass (Da):57,143
Last modified:July 5, 2004 - v1
Checksum:i2EB97E811ED9C0E6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti301 – 3011A → T in AAH80829 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068131 mRNA. Translation: AAH68131.1.
BC080829 mRNA. Translation: AAH80829.1.
CCDSiCCDS16548.1.
RefSeqiNP_997089.1. NM_207206.2.
UniGeneiMm.33438.

Genome annotation databases

EnsembliENSMUST00000028554; ENSMUSP00000028554; ENSMUSG00000027134.
GeneIDi99010.
KEGGimmu:99010.
UCSCiuc008loo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068131 mRNA. Translation: AAH68131.1.
BC080829 mRNA. Translation: AAH80829.1.
CCDSiCCDS16548.1.
RefSeqiNP_997089.1. NM_207206.2.
UniGeneiMm.33438.

3D structure databases

ProteinModelPortaliQ6NVG1.
SMRiQ6NVG1. Positions 351-399, 406-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028554.

PTM databases

iPTMnetiQ6NVG1.
PhosphoSiteiQ6NVG1.

Proteomic databases

EPDiQ6NVG1.
MaxQBiQ6NVG1.
PaxDbiQ6NVG1.
PeptideAtlasiQ6NVG1.
PRIDEiQ6NVG1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028554; ENSMUSP00000028554; ENSMUSG00000027134.
GeneIDi99010.
KEGGimmu:99010.
UCSCiuc008loo.1. mouse.

Organism-specific databases

CTDi254531.
MGIiMGI:2138993. Lpcat4.

Phylogenomic databases

eggNOGiKOG4666. Eukaryota.
ENOG410XSIQ. LUCA.
GeneTreeiENSGT00390000004914.
HOGENOMiHOG000234374.
InParanoidiQ6NVG1.
KOiK13512.
OMAiLRPPHTS.
OrthoDBiEOG7NCV5G.
PhylomeDBiQ6NVG1.
TreeFamiTF323244.

Enzyme and pathway databases

UniPathwayiUPA00085.
BRENDAi2.3.1.23. 3474.
ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482925. Acyl chain remodelling of PG.
R-MMU-1483166. Synthesis of PA.
R-MMU-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ6NVG1.
SOURCEiSearch...

Gene expression databases

BgeeiQ6NVG1.
GenevisibleiQ6NVG1. MM.

Family and domain databases

InterProiIPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Embryo.
  2. "Mammalian acyl-CoA:lysophosphatidylcholine acyltransferase enzymes."
    Soupene E., Fyrst H., Kuypers F.A.
    Proc. Natl. Acad. Sci. U.S.A. 105:88-93(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
  3. "Molecular identification of a novel mammalian brain isoform of acyl-CoA:lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2."
    Cao J., Shan D., Revett T., Li D., Wu L., Liu W., Tobin J.F., Gimeno R.E.
    J. Biol. Chem. 283:19049-19057(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiLPCT4_MOUSE
AccessioniPrimary (citable) accession number: Q6NVG1
Secondary accession number(s): Q66JP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.