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Protein

Breast cancer type 1 susceptibility protein homolog

Gene

Brca1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator.UniRule annotation

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycleUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, Ubl conjugation pathwayUniRule annotation

Keywords - Ligandi

DNA-bindingUniRule annotation, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Breast cancer type 1 susceptibility protein homologUniRule annotation (EC:2.3.2.27UniRule annotation)
Gene namesi
Name:Brca1Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:104537. Brca1.

Subcellular locationi

  • Nucleus UniRule annotation
  • Chromosome UniRule annotation

  • Note: Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

ChromosomeUniRule annotation, NucleusUniRule annotation

PTM / Processingi

Proteomic databases

PaxDbiQ6NV63.
PeptideAtlasiQ6NV63.

Expressioni

Gene expression databases

GenevisibleiQ6NV63. MM.

Interactioni

Subunit structurei

Heterodimer with BARD1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the MRE11-RAD50-NBN protein (MRN) complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1, BRCC3, BRE and BABAM1. Interacts (via the BRCT domains) with FAM175A. Component of the BRCA1-RBBP8 complex.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000017290.

Structurei

3D structure databases

ProteinModelPortaliQ6NV63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 65RING-typeInterPro annotationAdd BLAST42
Domaini1584 – 1678BRCTInterPro annotationAdd BLAST95
Domaini1697 – 1796BRCTInterPro annotationAdd BLAST100

Keywords - Domaini

Zinc-fingerSAAS annotation

Phylogenomic databases

eggNOGiENOG410ITQ6. Eukaryota.
ENOG4111WR7. LUCA.
HOVERGENiHBG050730.
KOiK10605.

Family and domain databases

CDDicd00027. BRCT. 2 hits.
Gene3Di3.30.40.10. 1 hit.
3.40.50.10190. 2 hits.
InterProiIPR011364. BRCA1.
IPR031099. BRCA1-associated.
IPR025994. BRCA1_serine_dom.
IPR001357. BRCT_dom.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR13763. PTHR13763. 1 hit.
PTHR13763:SF0. PTHR13763:SF0. 1 hit.
PfamiPF00533. BRCT. 2 hits.
PF12820. BRCT_assoc. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PIRSFiPIRSF001734. BRCA1. 1 hit.
PRINTSiPR00493. BRSTCANCERI.
SMARTiSM00292. BRCT. 2 hits.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 2 hits.
PROSITEiPS50172. BRCT. 2 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NV63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLSAVQIQE VQNVLHAMQK ILECPICLEL IKEPVSTKCD HIFCKFCMLK
60 70 80 90 100
LLNQKKGPSQ CPLCKNEITK RSLQGSTRFS QLAEELLRIM AAFELDTGMQ
110 120 130 140 150
LTNGFSFSKK RNNSCERLNE EASIIQSVGY RNRVRRLPQV EPGNATLKDS
160 170 180 190 200
LGVQLSNLGI VRSVKKNRQT QPRKKSVYIE LDSDSSEETV TKPGDCSVRD
210 220 230 240 250
QELLQTAPQE AGDEGKLHSA EEAACEFSGG IRNIEHHQCS DDLNPTENHA
260 270 280 290 300
TERHPEKCQS ISISNVCVEP CGTDAHASSL QPETSSLLLI EDRMNAEKAE
310 320 330 340 350
FCNKSKQPGI AVSQQSRWAA SKGTCNDRQV PSTGEKVGPN ADSLSDREKW
360 370 380 390 400
THPQSLCPEN SGATTDVPWI TLNSSVQKVN EWFSRTGEML TSDSASARRH
410 420 430 440 450
ESNAEAAVVL EVSNEVDGGF SSSRKTDLVT PDPHHTLMCK RGRDFSKPVE
460 470 480 490 500
DNISDKIFGK SYQRKGSRPH LNHVTEIIGT FITEPQITQE QPFTNKLKRK
510 520 530 540 550
RSTSLQPEDF IKKADSAGVQ RTPDNINQGT DLMEPNEQAV STTSNCQENK
560 570 580 590 600
IAGSNLQKEK SAHPTESLRK EPASTAGAKS ISNSVSDLEV ELNVHSSKAP
610 620 630 640 650
KKNRLRRKSS IRCALPLEPI SRNPSPPTCA ELQIDSCGSS EETKKNHSNQ
660 670 680 690 700
QPAGHLREPQ LIEDTEPAAD AKKNEPNEHI RKRRASDAFP EEKLMNKAGL
710 720 730 740 750
LTSCSSPRKS QGPVNPSPQR TGTEQLETRQ MSDSAKELGD RVLGGEPSGK
760 770 780 790 800
TTDRSEESTS VSLVSDTDYD TQNSVSVLDA HTVRYARTGS AQCMTQFVAS
810 820 830 840 850
ENPKELVHGS NNAGSGTEGL KPPLRHALNL SQEKVEMEDS ELDTQYLQNT
860 870 880 890 900
FQVSKRQSFA LFSKPRSPQK DCAHSVPSKE LSPKVTAKGK QKERQGQEEF
910 920 930 940 950
EISHVQAVAA TVGLPVPCQE GKLAADTMCD RGCRLCPSSH YRSGENGLSA
960 970 980 990 1000
TGKSGISQNS HFKQSVSPIR SSIKTDNRKP LTEGRFERHT PSTEMAVGNE
1010 1020 1030 1040 1050
NILQSTVHTV SLNNRGNACQ EAGSGSIHEV CSTGDSFPGQ LGRNRGPKVN
1060 1070 1080 1090 1100
TVPPLDSMQP GVCQQSVPVS DKYLEIKKQE GEAVCADFSP CLFSDHLEQS
1110 1120 1130 1140 1150
MSGKVFQVCS ETPDDLLDDV EIQGHTSFGE GDIMERSAVF NGSILRRESS
1160 1170 1180 1190 1200
RSPSPVTHAS KSQSLHRASR KLESSEESDS TEDEDLPCFQ HLLSRISNTP
1210 1220 1230 1240 1250
ELTKCSSAVA QRMPEKAEGT QAPWKGSSSD CNNEVIMIEA SQEHQFSEDP
1260 1270 1280 1290 1300
RCSGSMFSSQ HSAAQGSTAN ANSQDSNFIP PSKQRSHQCG NEEAFLSDKE
1310 1320 1330 1340 1350
LISDNEEMAT CLEEDNDQEE DSIIPDSEAS GYESETNLSE DCSQSDILTT
1360 1370 1380 1390 1400
QQRATMKYNL IKLQQEMAHL EAVLEQRGNQ PSGHSPSLLA DPCALEDLPD
1410 1420 1430 1440 1450
LEPNMSGAAI LTSKNINENP VSQNLKSACD DKFQLQHLEG PTSGDDESGM
1460 1470 1480 1490 1500
GRPSPFKSPL AGSRGSAHGC SRHLQKRNSP SQEELLQPAG SEASSEPHNS
1510 1520 1530 1540 1550
TGQSCLPRRE LGTPYLGSGI SLFSSRDPES ESPKEPAHIG TTPASTSALK
1560 1570 1580 1590 1600
IPQGQVAFRS AAAAGADKAV VGIVSKIKPE LTSSEERADR DISMVVSGLT
1610 1620 1630 1640 1650
PKEVMTVQKF AEKYRLTLTD AITEETTHVI IKTDAEFVCE RTLKYFLGIA
1660 1670 1680 1690 1700
GGKWIVSYSW VVRSIQERRL LNVHEFEVKG DVVTGRNHQG PRRSRESREK
1710 1720 1730 1740 1750
LFKGLQVYCC EPFTNMPKDE LERMLQLCGA SVVKELPSLT HDTGAHLVVI
1760 1770 1780 1790 1800
VQPSAWTEDS NCPDIGQLCK ARLVMWDWVL DSLSSYRCRD LDAYLVQNIT
1810
CDSSEPQDSN D
Length:1,811
Mass (Da):198,615
Last modified:July 5, 2004 - v1
Checksum:i76197BA09B8DEC86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068303 mRNA. Translation: AAH68303.1.
RefSeqiNP_033894.3. NM_009764.3.
UniGeneiMm.244975.

Genome annotation databases

GeneIDi12189.
KEGGimmu:12189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068303 mRNA. Translation: AAH68303.1.
RefSeqiNP_033894.3. NM_009764.3.
UniGeneiMm.244975.

3D structure databases

ProteinModelPortaliQ6NV63.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000017290.

Proteomic databases

PaxDbiQ6NV63.
PeptideAtlasiQ6NV63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi12189.
KEGGimmu:12189.

Organism-specific databases

CTDi672.
MGIiMGI:104537. Brca1.

Phylogenomic databases

eggNOGiENOG410ITQ6. Eukaryota.
ENOG4111WR7. LUCA.
HOVERGENiHBG050730.
KOiK10605.

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

GenevisibleiQ6NV63. MM.

Family and domain databases

CDDicd00027. BRCT. 2 hits.
Gene3Di3.30.40.10. 1 hit.
3.40.50.10190. 2 hits.
InterProiIPR011364. BRCA1.
IPR031099. BRCA1-associated.
IPR025994. BRCA1_serine_dom.
IPR001357. BRCT_dom.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR13763. PTHR13763. 1 hit.
PTHR13763:SF0. PTHR13763:SF0. 1 hit.
PfamiPF00533. BRCT. 2 hits.
PF12820. BRCT_assoc. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PIRSFiPIRSF001734. BRCA1. 1 hit.
PRINTSiPR00493. BRSTCANCERI.
SMARTiSM00292. BRCT. 2 hits.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 2 hits.
PROSITEiPS50172. BRCT. 2 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ6NV63_MOUSE
AccessioniPrimary (citable) accession number: Q6NV63
Entry historyi
Integrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.