Q6NV04 (ILVBL_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetolactate synthase-like protein
EC=2.2.1.-

Alternative name(s):
IlvB-like protein

Gene names

Name:	ilvbl
ORF Names:	zgc:66376, zgc:85697

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Subcellular location	Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Acetolactate synthase-like protein		PRO_0000314827
Regions
	Transmembrane	7 – 29	23	Helical; Potential
	Region	459 – 539	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	510	1	Magnesium By similarity
	Metal binding	536	1	Magnesium By similarity
	Binding site	87	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NV04 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B56AF5DB90B4A026	FASTA	621	67,036
	10 20 30 40 50 60 MDISMYLGCS LGAALGGVIF ASYKLGLLYQ LFHKTERQSP RHGGESVAEV LRSHGVKFVF 70 80 90 100 110 120 TLVGGHISPI LVACEKLGIR IVDTRHEATA VFAADAVARL SGTVGVAAVT AGPGLTNTVT 130 140 150 160 170 180 AVKNAQMAES PLLLIGGAAA TLLQGRGALQ DIDQMSLFKP LCKFCASVRT VREIVPTVRK 190 200 210 220 230 240 ALAIAQSGTP GPVFIEFPID TLYPYHVVEK EFAPKNTPKG LMGKIIAWYL KNHLSNLFAG 250 260 270 280 290 300 AWESRDLSPL PVHIPHATDD QVQRCVELVS RAKKPVILLG SQATLPPTPA DDIRKALESL 310 320 330 340 350 360 GIPCFLGGMS RGLLGKNSPL HIRQNRRDAL KDADLVLLAG TVCDFRLSYG RVLNRRSKII 370 380 390 400 410 420 AVNRDKSQLL KNSDMFWKPT VAIQGDAGSF LLNLSKALKG HRCPEEWPQS LKEGDNVKEK 430 440 450 460 470 480 ANRAKADEKT ERHLNPLSVL HRVDELLAED SIIVADGGDF VGSAAYIMRP RGPLCWLDPG 490 500 510 520 530 540 AFGTLGVGGG FALGAKLCRP ESEVWIVYGD GSLGYTVAEF DTFTRHKTPV IALVGNDACW 550 560 570 580 590 600 SQISREQVPM LGSNVACGLA FTDYHVVADG YGGKGYLIGR EDESQLEDII KKAQKECKEG 610 620 KAVLLNVLIG KTNFREGSIS V

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC068362 mRNA. Translation: AAH68362.1.
IPI	IPI00500139.
RefSeq	NP_956960.2. NM_200666.2.
UniGene	Dr.12240.
3D structure databases
HSSP	HSSP built from PDB template 1OZH based on UniProtKB P27696.
ProteinModelPortal	Q6NV04.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6NV04.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000016271; ENSDARP00000004404; ENSDARG00000017126.
GeneID	393639.
KEGG	dre:393639.
Organism-specific databases
CTD	10994.
ZFIN	ZDB-GENE-040426-1623. ilvbl.
Phylogenomic databases
GeneTree	ENSGT00530000063412.
HOGENOM	HBG323037.
HOVERGEN	HBG108004.
OMA	FLGGMSR.
OrthoDB	EOG48D0V2.
PhylomeDB	Q6NV04.
Gene expression databases
ArrayExpress	Q6NV04.
Bgee	Q6NV04.
Family and domain databases
InterPro	IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K11259.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVBL_DANRE

Accession

Primary (citable) accession number: Q6NV04

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program