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Protein

Transmembrane protein 214

Gene

TMEM214

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Critical mediator, in cooperation with CASP4, of endoplasmic reticulum-stress induced apoptosis. Required or the activation of CASP4 following endoplasmic reticulum stress.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protein 214
Gene namesi
Name:TMEM214
ORF Names:PP446
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:25983. TMEM214.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei480 – 50021HelicalSequence analysisAdd
BLAST
Transmembranei616 – 63621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162406505.

Polymorphism and mutation databases

BioMutaiTMEM214.
DMDMi189042272.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 689688Transmembrane protein 214PRO_0000321897Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence analysis
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

EPDiQ6NUQ4.
MaxQBiQ6NUQ4.
PaxDbiQ6NUQ4.
PRIDEiQ6NUQ4.

PTM databases

iPTMnetiQ6NUQ4.
PhosphoSiteiQ6NUQ4.
SwissPalmiQ6NUQ4.

Expressioni

Gene expression databases

BgeeiQ6NUQ4.
CleanExiHS_TMEM214.
ExpressionAtlasiQ6NUQ4. baseline and differential.
GenevisibleiQ6NUQ4. HS.

Organism-specific databases

HPAiHPA034551.

Interactioni

Subunit structurei

Constitutively interacts with CASP4; required for the localization of procaspase 4 to the ER.

Binary interactionsi

WithEntry#Exp.IntActNotes
LSM1O151161EBI-372318,EBI-347619

Protein-protein interaction databases

BioGridi120216. 46 interactions.
IntActiQ6NUQ4. 14 interactions.
STRINGi9606.ENSP00000238788.

Structurei

3D structure databases

ProteinModelPortaliQ6NUQ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TMEM214 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4467. Eukaryota.
ENOG410ZR2A. LUCA.
GeneTreeiENSGT00390000002693.
HOVERGENiHBG054145.
InParanoidiQ6NUQ4.
OMAiGFEKIMK.
OrthoDBiEOG7N0C46.
PhylomeDBiQ6NUQ4.
TreeFamiTF329489.

Family and domain databases

InterProiIPR019308. TMEM214.
[Graphical view]
PANTHERiPTHR13448. PTHR13448. 1 hit.
PfamiPF10151. TMEM214. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NUQ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATKTAGVGR WEVVKKGRRP GVGAGAGGRG GGRNRRALGE ANGVWKYDLT
60 70 80 90 100
PAIQTTSTLY ERGFENIMKR QNKEQVPPPA VEPKKPGNKK QPKKVATPPN
110 120 130 140 150
QNQKQGRFRS LEEALKALDV ADLQKELDKS QSVFSGNPSI WLKDLASYLN
160 170 180 190 200
YKLQAPLSEP TLSQHTHDYP YSLVSRELRG IIRGLLAKAA GSLELFFDHC
210 220 230 240 250
LFTMLQELDK TPGESLHGYR ICIQAILQDK PKIATANLGK FLELLRSHQS
260 270 280 290 300
RPAKCLTIMW ALGQAGFANL TEGLKVWLGI MLPVLGIKSL SPFAITYLDR
310 320 330 340 350
LLLMHPNLTK GFGMIGPKDF FPLLDFAYMP NNSLTPSLQE QLCQLYPRLK
360 370 380 390 400
VLAFGAKPDS TLHTYFPSFL SRATPSCPPE MKKELLSSLT ECLTVDPLSA
410 420 430 440 450
SVWRQLYPKH LSQSSLLLEH LLSSWEQIPK KVQKSLQETI QSLKLTNQEL
460 470 480 490 500
LRKGSSNNQD VVTCDMACKG LLQQVQGPRL PWTRLLLLLL VFAVGFLCHD
510 520 530 540 550
LRSHSSFQAS LTGRLLRSSG FLPASQQACA KLYSYSLQGY SWLGETLPLW
560 570 580 590 600
GSHLLTVVRP SLQLAWAHTN ATVSFLSAHC ASHLAWFGDS LTSLSQRLQI
610 620 630 640 650
QLPDSVNQLL RYLRELPLLF HQNVLLPLWH LLLEALAWAQ EHCHEACRGE
660 670 680
VTWDCMKTQL SEAVHWTWLC LQDITVAFLD WALALISQQ
Length:689
Mass (Da):77,151
Last modified:February 26, 2008 - v2
Checksum:iA12259676BA5B8E4
GO
Isoform 2 (identifier: Q6NUQ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-212: Missing.

Show »
Length:644
Mass (Da):72,040
Checksum:i3239DD7F41C1623C
GO

Sequence cautioni

The sequence AAL55739.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA91038.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC11331.1 differs from that shown. Reason: Frameshift at position 94. Curated
The sequence CAH10591.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 991P → H in AAH68479 (PubMed:15489334).Curated
Sequence conflicti138 – 1381P → Q in AAH68479 (PubMed:15489334).Curated
Sequence conflicti326 – 3261F → Y in BAA91038 (PubMed:14702039).Curated
Sequence conflicti502 – 5021R → W in AAL55739 (PubMed:15498874).Curated
Sequence conflicti651 – 6511V → A in BAG53451 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti351 – 3511V → M.
Corresponds to variant rs1124649 [ dbSNP | Ensembl ].
VAR_039370

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei168 – 21245Missing in isoform 2. 1 PublicationVSP_041155Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000261 mRNA. Translation: BAA91038.1. Different initiation.
AK074981 mRNA. Translation: BAC11331.1. Frameshift.
AK097308 mRNA. Translation: BAG53451.1.
AC013472 Genomic DNA. Translation: AAY14654.1.
CH471053 Genomic DNA. Translation: EAX00655.1.
CH471053 Genomic DNA. Translation: EAX00657.1.
CH471053 Genomic DNA. Translation: EAX00659.1.
AL833725 mRNA. Translation: CAH10591.1. Different initiation.
BC068479 mRNA. Translation: AAH68479.1.
AF289555 mRNA. Translation: AAL55739.1. Different initiation.
CCDSiCCDS42664.1. [Q6NUQ4-1]
CCDS46242.1. [Q6NUQ4-2]
RefSeqiNP_001077059.1. NM_001083590.1. [Q6NUQ4-2]
NP_060197.4. NM_017727.4. [Q6NUQ4-1]
UniGeneiHs.533934.

Genome annotation databases

EnsembliENST00000238788; ENSP00000238788; ENSG00000119777. [Q6NUQ4-1]
ENST00000321326; ENSP00000319469; ENSG00000119777. [Q6NUQ4-1]
ENST00000404032; ENSP00000384417; ENSG00000119777. [Q6NUQ4-2]
GeneIDi54867.
KEGGihsa:54867.
UCSCiuc002ria.5. human. [Q6NUQ4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000261 mRNA. Translation: BAA91038.1. Different initiation.
AK074981 mRNA. Translation: BAC11331.1. Frameshift.
AK097308 mRNA. Translation: BAG53451.1.
AC013472 Genomic DNA. Translation: AAY14654.1.
CH471053 Genomic DNA. Translation: EAX00655.1.
CH471053 Genomic DNA. Translation: EAX00657.1.
CH471053 Genomic DNA. Translation: EAX00659.1.
AL833725 mRNA. Translation: CAH10591.1. Different initiation.
BC068479 mRNA. Translation: AAH68479.1.
AF289555 mRNA. Translation: AAL55739.1. Different initiation.
CCDSiCCDS42664.1. [Q6NUQ4-1]
CCDS46242.1. [Q6NUQ4-2]
RefSeqiNP_001077059.1. NM_001083590.1. [Q6NUQ4-2]
NP_060197.4. NM_017727.4. [Q6NUQ4-1]
UniGeneiHs.533934.

3D structure databases

ProteinModelPortaliQ6NUQ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120216. 46 interactions.
IntActiQ6NUQ4. 14 interactions.
STRINGi9606.ENSP00000238788.

PTM databases

iPTMnetiQ6NUQ4.
PhosphoSiteiQ6NUQ4.
SwissPalmiQ6NUQ4.

Polymorphism and mutation databases

BioMutaiTMEM214.
DMDMi189042272.

Proteomic databases

EPDiQ6NUQ4.
MaxQBiQ6NUQ4.
PaxDbiQ6NUQ4.
PRIDEiQ6NUQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000238788; ENSP00000238788; ENSG00000119777. [Q6NUQ4-1]
ENST00000321326; ENSP00000319469; ENSG00000119777. [Q6NUQ4-1]
ENST00000404032; ENSP00000384417; ENSG00000119777. [Q6NUQ4-2]
GeneIDi54867.
KEGGihsa:54867.
UCSCiuc002ria.5. human. [Q6NUQ4-1]

Organism-specific databases

CTDi54867.
GeneCardsiTMEM214.
HGNCiHGNC:25983. TMEM214.
HPAiHPA034551.
MIMi615301. gene.
neXtProtiNX_Q6NUQ4.
PharmGKBiPA162406505.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4467. Eukaryota.
ENOG410ZR2A. LUCA.
GeneTreeiENSGT00390000002693.
HOVERGENiHBG054145.
InParanoidiQ6NUQ4.
OMAiGFEKIMK.
OrthoDBiEOG7N0C46.
PhylomeDBiQ6NUQ4.
TreeFamiTF329489.

Miscellaneous databases

ChiTaRSiTMEM214. human.
GenomeRNAii54867.
NextBioi57770.
PROiQ6NUQ4.
SOURCEiSearch...

Gene expression databases

BgeeiQ6NUQ4.
CleanExiHS_TMEM214.
ExpressionAtlasiQ6NUQ4. baseline and differential.
GenevisibleiQ6NUQ4. HS.

Family and domain databases

InterProiIPR019308. TMEM214.
[Graphical view]
PANTHERiPTHR13448. PTHR13448. 1 hit.
PfamiPF10151. TMEM214. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Colon mucosa and Stomach.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-689 (ISOFORM 1).
    Tissue: Amygdala and Stomach.
  6. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 286-689 (ISOFORM 1).
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Transmembrane protein 214 (TMEM214) mediates endoplasmic reticulum stress-induced caspase 4 enzyme activation and apoptosis."
    Li C., Wei J., Li Y., He X., Zhou Q., Yan J., Zhang J., Liu Y., Liu Y., Shu H.B.
    J. Biol. Chem. 288:17908-17917(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTM214_HUMAN
AccessioniPrimary (citable) accession number: Q6NUQ4
Secondary accession number(s): A6NNF2
, B3KUI9, B5MCD8, D6W547, Q53SW1, Q69YH4, Q8NC45, Q8WZ37, Q9NXH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: May 11, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.