Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase 4 regulatory subunit 4

Gene

PPP4R4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative regulatory subunit of serine/threonine-protein phosphatase 4.

GO - Molecular functioni

  • protein phosphatase regulator activity Source: MGI

GO - Biological processi

  • negative regulation of phosphoprotein phosphatase activity Source: MGI
  • regulation of protein serine/threonine phosphatase activity Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 regulatory subunit 4
Gene namesi
Name:PPP4R4
Synonyms:KIAA1622, PP4R4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:23788. PPP4R4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • protein serine/threonine phosphatase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi501 – 5011R → W: Abolishes interaction with PPP4C. 1 Publication
Mutagenesisi618 – 6181V → A: Diminishes interaction with PPP4C. 1 Publication
Mutagenesisi618 – 6181V → D: Abolishes interaction with PPP4C. 1 Publication

Organism-specific databases

PharmGKBiPA164724938.

Polymorphism and mutation databases

BioMutaiPPP4R4.
DMDMi74736832.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Serine/threonine-protein phosphatase 4 regulatory subunit 4PRO_0000311850Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei775 – 7751PhosphoserineBy similarity
Modified residuei797 – 7971PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6NUP7.
PaxDbiQ6NUP7.
PRIDEiQ6NUP7.

PTM databases

iPTMnetiQ6NUP7.
PhosphoSiteiQ6NUP7.

Expressioni

Gene expression databases

BgeeiQ6NUP7.
CleanExiHS_PPP4R4.
ExpressionAtlasiQ6NUP7. baseline and differential.
GenevisibleiQ6NUP7. HS.

Organism-specific databases

HPAiHPA041866.

Interactioni

Subunit structurei

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP4CP605106EBI-1774189,EBI-1046072

Protein-protein interaction databases

BioGridi121741. 2 interactions.
IntActiQ6NUP7. 3 interactions.
MINTiMINT-4905889.
STRINGi9606.ENSP00000305924.

Structurei

3D structure databases

ProteinModelPortaliQ6NUP7.
SMRiQ6NUP7. Positions 130-300, 399-460, 606-632.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati213 – 25139HEAT 1Add
BLAST
Repeati252 – 29039HEAT 2Add
BLAST
Repeati392 – 42736HEAT 3Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili686 – 72035Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 3 HEAT repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IUDH. Eukaryota.
ENOG410XPMD. LUCA.
GeneTreeiENSGT00510000047895.
HOGENOMiHOG000115653.
HOVERGENiHBG098096.
InParanoidiQ6NUP7.
KOiK15426.
OMAiLYGIFTP.
OrthoDBiEOG7QVM20.
PhylomeDBiQ6NUP7.
TreeFamiTF313717.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NUP7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHPPPPAAAM DFSQNSLFGY MEDLQELTII ERPVRRSLKT PEEIERLTVD
60 70 80 90 100
EDLSDIERAV YLLSAGQDVQ GTSVIANLPF LMRQNPTETL RRVLPKVREA
110 120 130 140 150
LHVAGVEMQL TAAMSFLTIL QDESVSIHAY THSFLQVILL HLEHRDTGVS
160 170 180 190 200
NAWLETLLSV IEVLPKETLR HEILNPLVSK AQLSQTVQSR LVSCKILGKL
210 220 230 240 250
TNKFDAHTIK REILPLVKSL CQDVEYEVRS CMCRQLENIA QGIGTELTKS
260 270 280 290 300
VVLPELIELS RDEGSSVRLA AFETLVNLLD IFDTDDRSQT ILPLVKSFCE
310 320 330 340 350
KSFKADESIL ISLSFHLGKL CHGLYGIFTP DQHLRFLEFY KKLCTLGLQQ
360 370 380 390 400
ENGHNENQIP PQILEQEKKY ISVRKNCAYN FPAMIVFVDP KNFHMELYST
410 420 430 440 450
FFCLCHDPEV PVRYTIAICF YEVSKLLNSG VYLIHKELIT LLQDESLEVL
460 470 480 490 500
DALIDHLPEI LELMSTGGES SVQENKLSSL PDLIPALTAA EQRAAASLKW
510 520 530 540 550
RTHEKLLQKY ACLPHVISSD QIYYRFLQRM FTIMMTNNVL PVQKAASRTL
560 570 580 590 600
CIFLRYNRKQ EQRHEVIQKL IEQLGQGKSY WNRLRFLDTC EFIIEIFSKS
610 620 630 640 650
FFCKYFFLPA IELTHDPVAN VRMKLCYLLP KVKSTLKIPA DKHLLQQLEM
660 670 680 690 700
CVRKLLCQEK DKDVLAIVKR TVLELDRMEM SMDAFQKKFY EKDLLDQEKE
710 720 730 740 750
REELLLLEME QLEKEKQQND GRPMSDKMFE KKRRDTKTPT QSLPKNIPIS
760 770 780 790 800
VPGPSSVTPS TSKEIKKSKL IRSQSFNNQA FHAKYGNLEK CASKSSTTGY
810 820 830 840 850
TTSVSGLGKT SVLSLADDSF RTRNASSVPS SFSPNTPLPS TSRGTGNSVD
860 870
PKSSGSKDTQ PRKATLKSRK SNP
Length:873
Mass (Da):99,452
Last modified:July 5, 2004 - v1
Checksum:i9C1A9CD4584447C1
GO
Isoform 2 (identifier: Q6NUP7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     99-120: EALHVAGVEMQLTAAMSFLTIL → VHEDAHLFIQRVWISHMFVQRV
     121-873: Missing.

Note: No experimental confirmation available.
Show »
Length:120
Mass (Da):13,894
Checksum:i0B935EB2909EBB6A
GO

Sequence cautioni

The sequence BAB13448.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei99 – 12022EALHV…FLTIL → VHEDAHLFIQRVWISHMFVQ RV in isoform 2. 1 PublicationVSP_029616Add
BLAST
Alternative sequencei121 – 873753Missing in isoform 2. 1 PublicationVSP_029617Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046842 mRNA. Translation: BAB13448.1. Different initiation.
CH471061 Genomic DNA. Translation: EAW81561.1.
CH471061 Genomic DNA. Translation: EAW81562.1.
BC002650 mRNA. Translation: AAH02650.2.
BC068491 mRNA. Translation: AAH68491.1.
CCDSiCCDS9921.1. [Q6NUP7-1]
CCDS9922.1. [Q6NUP7-2]
RefSeqiNP_066009.2. NM_020958.2. [Q6NUP7-2]
NP_478144.1. NM_058237.1. [Q6NUP7-1]
UniGeneiHs.259599.

Genome annotation databases

EnsembliENST00000304338; ENSP00000305924; ENSG00000119698. [Q6NUP7-1]
ENST00000328839; ENSP00000330831; ENSG00000119698. [Q6NUP7-2]
ENST00000614957; ENSP00000482849; ENSG00000278326. [Q6NUP7-2]
ENST00000619905; ENSP00000480902; ENSG00000278326. [Q6NUP7-1]
GeneIDi57718.
KEGGihsa:57718.
UCSCiuc001ycr.4. human. [Q6NUP7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046842 mRNA. Translation: BAB13448.1. Different initiation.
CH471061 Genomic DNA. Translation: EAW81561.1.
CH471061 Genomic DNA. Translation: EAW81562.1.
BC002650 mRNA. Translation: AAH02650.2.
BC068491 mRNA. Translation: AAH68491.1.
CCDSiCCDS9921.1. [Q6NUP7-1]
CCDS9922.1. [Q6NUP7-2]
RefSeqiNP_066009.2. NM_020958.2. [Q6NUP7-2]
NP_478144.1. NM_058237.1. [Q6NUP7-1]
UniGeneiHs.259599.

3D structure databases

ProteinModelPortaliQ6NUP7.
SMRiQ6NUP7. Positions 130-300, 399-460, 606-632.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121741. 2 interactions.
IntActiQ6NUP7. 3 interactions.
MINTiMINT-4905889.
STRINGi9606.ENSP00000305924.

PTM databases

iPTMnetiQ6NUP7.
PhosphoSiteiQ6NUP7.

Polymorphism and mutation databases

BioMutaiPPP4R4.
DMDMi74736832.

Proteomic databases

MaxQBiQ6NUP7.
PaxDbiQ6NUP7.
PRIDEiQ6NUP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304338; ENSP00000305924; ENSG00000119698. [Q6NUP7-1]
ENST00000328839; ENSP00000330831; ENSG00000119698. [Q6NUP7-2]
ENST00000614957; ENSP00000482849; ENSG00000278326. [Q6NUP7-2]
ENST00000619905; ENSP00000480902; ENSG00000278326. [Q6NUP7-1]
GeneIDi57718.
KEGGihsa:57718.
UCSCiuc001ycr.4. human. [Q6NUP7-1]

Organism-specific databases

CTDi57718.
GeneCardsiPPP4R4.
HGNCiHGNC:23788. PPP4R4.
HPAiHPA041866.
neXtProtiNX_Q6NUP7.
PharmGKBiPA164724938.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IUDH. Eukaryota.
ENOG410XPMD. LUCA.
GeneTreeiENSGT00510000047895.
HOGENOMiHOG000115653.
HOVERGENiHBG098096.
InParanoidiQ6NUP7.
KOiK15426.
OMAiLYGIFTP.
OrthoDBiEOG7QVM20.
PhylomeDBiQ6NUP7.
TreeFamiTF313717.

Miscellaneous databases

GenomeRNAii57718.
NextBioi64648.
PROiQ6NUP7.

Gene expression databases

BgeeiQ6NUP7.
CleanExiHS_PPP4R4.
ExpressionAtlasiQ6NUP7. baseline and differential.
GenevisibleiQ6NUP7. HS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O.
    DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Uterus.
  4. "PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4."
    Chen G.I., Tisayakorn S., Jorgensen C., D'Ambrosio L.M., Goudreault M., Gingras A.-C.
    J. Biol. Chem. 283:29273-29284(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPP4C, IDENTIFICATION IN THE PPP4C-PPP4R4 COMPLEX, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-501 AND VAL-618.

Entry informationi

Entry nameiPP4R4_HUMAN
AccessioniPrimary (citable) accession number: Q6NUP7
Secondary accession number(s): Q9BUF8, Q9HCF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: July 5, 2004
Last modified: March 16, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.