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Protein

Acyl-coenzyme A synthetase ACSM5, mitochondrial

Gene

ACSM5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro) (By similarity).By similarity

Catalytic activityi

ATP + a carboxylate + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei455ATPBy similarity1
Binding sitei470ATPBy similarity1
Binding sitei566ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi230 – 238ATPBy similarity9
Nucleotide bindingi368 – 373ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS11973-MONOMER.
ReactomeiR-HSA-177128. Conjugation of salicylate with glycine.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A synthetase ACSM5, mitochondrial (EC:6.2.1.2)
Alternative name(s):
Acyl-CoA synthetase medium-chain family member 5
Gene namesi
Name:ACSM5
Synonyms:MACS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:26060. ACSM5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000183549.
PharmGKBiPA162375501.

Polymorphism and mutation databases

BioMutaiACSM5.
DMDMi269849538.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
ChainiPRO_000030610127 – 579Acyl-coenzyme A synthetase ACSM5, mitochondrialAdd BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97N6-acetyllysine; alternateBy similarity1
Modified residuei97N6-succinyllysine; alternateBy similarity1
Modified residuei152N6-acetyllysineBy similarity1
Modified residuei303N6-acetyllysine; alternateBy similarity1
Modified residuei303N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ6NUN0.
PeptideAtlasiQ6NUN0.
PRIDEiQ6NUN0.

PTM databases

iPTMnetiQ6NUN0.
PhosphoSitePlusiQ6NUN0.

Expressioni

Tissue specificityi

Detected in kidney and liver.1 Publication

Gene expression databases

BgeeiENSG00000183549.
CleanExiHS_ACSM5.
ExpressionAtlasiQ6NUN0. baseline and differential.
GenevisibleiQ6NUN0. HS.

Organism-specific databases

HPAiHPA041435.

Interactioni

Protein-protein interaction databases

BioGridi120323. 15 interactors.
IntActiQ6NUN0. 1 interactor.
STRINGi9606.ENSP00000327916.

Structurei

3D structure databases

ProteinModelPortaliQ6NUN0.
SMRiQ6NUN0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOGENOMiHOG000229982.
HOVERGENiHBG053031.
InParanoidiQ6NUN0.
KOiK01896.
OMAiCFFNCYL.
OrthoDBiEOG091G03NW.
PhylomeDBiQ6NUN0.
TreeFamiTF354287.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NUN0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRPWLRHLVL QALRNSRAFC GSHGKPAPLP VPQKIVATWE AISLGRQLVP
60 70 80 90 100
EYFNFAHDVL DVWSRLEEAG HRPPNPAFWW VNGTGAEIKW SFEELGKQSR
110 120 130 140 150
KAANVLGGAC GLQPGDRMML VLPRLPEWWL VSVACMRTGT VMIPGVTQLT
160 170 180 190 200
EKDLKYRLQA SRAKSIITSD SLAPRVDAIS AECPSLQTKL LVSDSSRPGW
210 220 230 240 250
LNFRELLREA STEHNCMRTK SRDPLAIYFT SGTTGAPKMV EHSQSSYGLG
260 270 280 290 300
FVASGRRWVA LTESDIFWNT TDTGWVKAAW TLFSAWPNGS CIFVHELPRV
310 320 330 340 350
DAKVILNTLS KFPITTLCCV PTIFRLLVQE DLTRYQFQSL RHCLTGGEAL
360 370 380 390 400
NPDVREKWKH QTGVELYEGY GQSETVVICA NPKGMKIKSG SMGKASPPYD
410 420 430 440 450
VQIVDDEGNV LPPGEEGNVA VRIRPTRPFC FFNCYLDNPE KTAASEQGDF
460 470 480 490 500
YITGDRARMD KDGYFWFMGR NDDVINSSSY RIGPVEVESA LAEHPAVLES
510 520 530 540 550
AVVSSPDPIR GEVVKAFIVL TPAYSSHDPE ALTRELQEHV KRVTAPYKYP
560 570
RKVAFVSELP KTVSGKIQRS KLRSQEWGK
Length:579
Mass (Da):64,760
Last modified:November 24, 2009 - v2
Checksum:i9493CB853CE29CB1
GO
Isoform 2 (identifier: Q6NUN0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     209-579: Missing.

Note: No experimental confirmation available.
Show »
Length:208
Mass (Da):23,320
Checksum:i5C29746BE07D3E30
GO

Sequence cautioni

The sequence BAA91273 differs from that shown. Reason: Frameshift at position 560.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142M → V in AAH16703 (PubMed:15489334).Curated1
Sequence conflicti231 – 234SGTT → KREPP in BAA91273 (PubMed:14702039).Curated4
Sequence conflicti502V → A in AAH68516 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05549565R → Q.Corresponds to variant rs9928053dbSNPEnsembl.1
Natural variantiVAR_035252159Q → H.1 PublicationCorresponds to variant rs559741756dbSNPEnsembl.1
Natural variantiVAR_055496182E → K.Corresponds to variant rs7192210dbSNPEnsembl.1
Natural variantiVAR_061011217M → V.Corresponds to variant rs59025904dbSNPEnsembl.1
Natural variantiVAR_035253352P → R.1 PublicationCorresponds to variant rs8062344dbSNPEnsembl.1
Natural variantiVAR_035254360H → R.1 PublicationCorresponds to variant rs12931877dbSNPEnsembl.1
Natural variantiVAR_035255533T → M.1 PublicationCorresponds to variant rs56308819dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028398209 – 579Missing in isoform 2. 1 PublicationAdd BLAST371

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000588 mRNA. Translation: BAA91273.1. Frameshift.
AC137056 Genomic DNA. No translation available.
BC013753 mRNA. Translation: AAH13753.1.
BC016703 mRNA. Translation: AAH16703.1.
BC068516 mRNA. Translation: AAH68516.1.
CCDSiCCDS10585.1. [Q6NUN0-1]
CCDS81954.1. [Q6NUN0-2]
RefSeqiNP_001311300.1. NM_001324371.1. [Q6NUN0-1]
NP_001311302.1. NM_001324373.1. [Q6NUN0-2]
NP_060358.2. NM_017888.2. [Q6NUN0-1]
UniGeneiHs.659606.

Genome annotation databases

EnsembliENST00000331849; ENSP00000327916; ENSG00000183549. [Q6NUN0-1]
ENST00000575584; ENSP00000460112; ENSG00000183549. [Q6NUN0-2]
GeneIDi54988.
KEGGihsa:54988.
UCSCiuc002dhd.1. human. [Q6NUN0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000588 mRNA. Translation: BAA91273.1. Frameshift.
AC137056 Genomic DNA. No translation available.
BC013753 mRNA. Translation: AAH13753.1.
BC016703 mRNA. Translation: AAH16703.1.
BC068516 mRNA. Translation: AAH68516.1.
CCDSiCCDS10585.1. [Q6NUN0-1]
CCDS81954.1. [Q6NUN0-2]
RefSeqiNP_001311300.1. NM_001324371.1. [Q6NUN0-1]
NP_001311302.1. NM_001324373.1. [Q6NUN0-2]
NP_060358.2. NM_017888.2. [Q6NUN0-1]
UniGeneiHs.659606.

3D structure databases

ProteinModelPortaliQ6NUN0.
SMRiQ6NUN0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120323. 15 interactors.
IntActiQ6NUN0. 1 interactor.
STRINGi9606.ENSP00000327916.

PTM databases

iPTMnetiQ6NUN0.
PhosphoSitePlusiQ6NUN0.

Polymorphism and mutation databases

BioMutaiACSM5.
DMDMi269849538.

Proteomic databases

PaxDbiQ6NUN0.
PeptideAtlasiQ6NUN0.
PRIDEiQ6NUN0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331849; ENSP00000327916; ENSG00000183549. [Q6NUN0-1]
ENST00000575584; ENSP00000460112; ENSG00000183549. [Q6NUN0-2]
GeneIDi54988.
KEGGihsa:54988.
UCSCiuc002dhd.1. human. [Q6NUN0-1]

Organism-specific databases

CTDi54988.
GeneCardsiACSM5.
H-InvDBHIX0023110.
HGNCiHGNC:26060. ACSM5.
HPAiHPA041435.
MIMi614361. gene.
neXtProtiNX_Q6NUN0.
OpenTargetsiENSG00000183549.
PharmGKBiPA162375501.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOGENOMiHOG000229982.
HOVERGENiHBG053031.
InParanoidiQ6NUN0.
KOiK01896.
OMAiCFFNCYL.
OrthoDBiEOG091G03NW.
PhylomeDBiQ6NUN0.
TreeFamiTF354287.

Enzyme and pathway databases

BioCyciZFISH:HS11973-MONOMER.
ReactomeiR-HSA-177128. Conjugation of salicylate with glycine.

Miscellaneous databases

GenomeRNAii54988.
PROiQ6NUN0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000183549.
CleanExiHS_ACSM5.
ExpressionAtlasiQ6NUN0. baseline and differential.
GenevisibleiQ6NUN0. HS.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACSM5_HUMAN
AccessioniPrimary (citable) accession number: Q6NUN0
Secondary accession number(s): Q96AV1, Q96CX8, Q9NWV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: November 24, 2009
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.