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Protein

All-trans-retinol 13,14-reductase

Gene

RETSAT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Retinol saturase carrying out the saturation of the 13-14 double bond of all-trans-retinol to produce all-trans-13,14-dihydroretinol. Has activity toward all-trans-retinol as substrate. Does not use all-trans-retinoic acid nor 9-cis, 11-cis or 13-cis-retinol isomers as substrates. May play a role in the metabolism of vitamin A (By similarity).By similarity

Catalytic activityi

All-trans-13,14-dihydroretinol + acceptor = all-trans-retinol + reduced acceptor.

Cofactori

NAD+By similarity, NADP+By similarity, FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi70 – 9829FAD or NAD or NADPSequence analysisAdd
BLAST

GO - Molecular functioni

  • all-trans-retinol 13,14-reductase activity Source: HGNC
  • oxidoreductase activity Source: HGNC

GO - Biological processi

  • oxidation-reduction process Source: HGNC
  • retinol metabolic process Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
All-trans-retinol 13,14-reductase (EC:1.3.99.23)
Alternative name(s):
All-trans-13,14-dihydroretinol saturase
Short name:
RetSat
PPAR-alpha-regulated and starvation-induced gene protein
Gene namesi
Name:RETSAT
Synonyms:PPSIG
ORF Names:UNQ439/PRO872
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:25991. RETSAT.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: HGNC
  • membrane Source: UniProtKB
  • nuclear membrane Source: HGNC
  • nuclear outer membrane Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA145007867.

Chemistry

DrugBankiDB00162. Vitamin A.

Polymorphism and mutation databases

BioMutaiRETSAT.
DMDMi90108452.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 610592All-trans-retinol 13,14-reductasePRO_0000225665Add
BLAST

Proteomic databases

EPDiQ6NUM9.
MaxQBiQ6NUM9.
PaxDbiQ6NUM9.
PeptideAtlasiQ6NUM9.
PRIDEiQ6NUM9.

PTM databases

iPTMnetiQ6NUM9.
PhosphoSiteiQ6NUM9.
SwissPalmiQ6NUM9.

Expressioni

Gene expression databases

BgeeiENSG00000042445.
CleanExiHS_RETSAT.
ExpressionAtlasiQ6NUM9. baseline and differential.
GenevisibleiQ6NUM9. HS.

Organism-specific databases

HPAiHPA007961.
HPA046513.

Interactioni

Protein-protein interaction databases

BioGridi120232. 26 interactions.
IntActiQ6NUM9. 1 interaction.
MINTiMINT-3039422.
STRINGi9606.ENSP00000295802.

Structurei

3D structure databases

ProteinModelPortaliQ6NUM9.
SMRiQ6NUM9. Positions 31-109, 309-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4254. Eukaryota.
COG1233. LUCA.
GeneTreeiENSGT00390000017613.
HOVERGENiHBG079484.
InParanoidiQ6NUM9.
KOiK09516.
OMAiFSMHALL.
OrthoDBiEOG091G04VP.
PhylomeDBiQ6NUM9.
TreeFamiTF328375.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NUM9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWLPLVLLLA VLLLAVLCKV YLGLFSGSSP NPFSEDVKRP PAPLVTDKEA
60 70 80 90 100
RKKVLKQAFS ANQVPEKLDV VVIGSGFGGL AAAAILAKAG KRVLVLEQHT
110 120 130 140 150
KAGGCCHTFG KNGLEFDTGI HYIGRMEEGS IGRFILDQIT EGQLDWAPLS
160 170 180 190 200
SPFDIMVLEG PNGRKEYPMY SGEKAYIQGL KEKFPQEEAI IDKYIKLVKV
210 220 230 240 250
VSSGAPHAIL LKFLPLPVVQ LLDRCGLLTR FSPFLQASTQ SLAEVLQQLG
260 270 280 290 300
ASSELQAVLS YIFPTYGVTP NHSAFSMHAL LVNHYMKGGF YPRGGSSEIA
310 320 330 340 350
FHTIPVIQRA GGAVLTKATV QSVLLDSAGK ACGVSVKKGH ELVNIYCPIV
360 370 380 390 400
VSNAGLFNTY EHLLPGNARC LPGVKQQLGT VRPGLGMTSV FICLRGTKED
410 420 430 440 450
LHLPSTNYYV YYDTDMDQAM ERYVSMPREE AAEHIPLLFF AFPSAKDPTW
460 470 480 490 500
EDRFPGRSTM IMLIPTAYEW FEEWQAELKG KRGSDYETFK NSFVEASMSV
510 520 530 540 550
VLKLFPQLEG KVESVTAGSP LTNQFYLAAP RGACYGADHD LGRLHPCVMA
560 570 580 590 600
SLRAQSPIPN LYLTGQDIFT CGLVGALQGA LLCSSAILKR NLYSDLKNLD
610
SRIRAQKKKN
Length:610
Mass (Da):66,820
Last modified:March 7, 2006 - v2
Checksum:i06AE8EBC16BBA2EC
GO
Isoform 2 (identifier: Q6NUM9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     457-481: RSTMIMLIPTAYEWFEEWQAELKGK → GECDCRIPTHQPVLSGCSPRCLLRG
     482-610: Missing.

Note: No experimental confirmation available.
Show »
Length:481
Mass (Da):52,439
Checksum:i6266754EA5A201E4
GO

Sequence cautioni

The sequence AAH11418 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA91069 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1401T → S in AAH68517 (PubMed:15489334).Curated
Sequence conflicti298 – 2981E → Q in AAH11418 (PubMed:15489334).Curated
Sequence conflicti353 – 3531N → S in BAC11505 (PubMed:16303743).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti533 – 5331A → V.
Corresponds to variant rs4832169 [ dbSNP | Ensembl ].
VAR_025473
Natural varianti536 – 5361G → R.
Corresponds to variant rs4832168 [ dbSNP | Ensembl ].
VAR_059243
Natural varianti559 – 5591P → T.
Corresponds to variant rs13384912 [ dbSNP | Ensembl ].
VAR_059244

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei457 – 48125RSTMI…ELKGK → GECDCRIPTHQPVLSGCSPR CLLRG in isoform 2. 1 PublicationVSP_017410Add
BLAST
Alternative sequencei482 – 610129Missing in isoform 2. 1 PublicationVSP_017411Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358568 mRNA. Translation: AAQ88931.1.
AK075261 mRNA. Translation: BAC11505.1.
AC062037 Genomic DNA. Translation: AAY24126.1.
AC093162 Genomic DNA. Translation: AAY24096.1.
BC068517 mRNA. Translation: AAH68517.1.
BC011418 mRNA. Translation: AAH11418.1. Different initiation.
AK000303 mRNA. Translation: BAA91069.1. Different initiation.
CCDSiCCDS1972.1. [Q6NUM9-1]
RefSeqiNP_060220.3. NM_017750.3. [Q6NUM9-1]
UniGeneiHs.440401.

Genome annotation databases

EnsembliENST00000295802; ENSP00000295802; ENSG00000042445. [Q6NUM9-1]
GeneIDi54884.
KEGGihsa:54884.
UCSCiuc002spd.4. human. [Q6NUM9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358568 mRNA. Translation: AAQ88931.1.
AK075261 mRNA. Translation: BAC11505.1.
AC062037 Genomic DNA. Translation: AAY24126.1.
AC093162 Genomic DNA. Translation: AAY24096.1.
BC068517 mRNA. Translation: AAH68517.1.
BC011418 mRNA. Translation: AAH11418.1. Different initiation.
AK000303 mRNA. Translation: BAA91069.1. Different initiation.
CCDSiCCDS1972.1. [Q6NUM9-1]
RefSeqiNP_060220.3. NM_017750.3. [Q6NUM9-1]
UniGeneiHs.440401.

3D structure databases

ProteinModelPortaliQ6NUM9.
SMRiQ6NUM9. Positions 31-109, 309-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120232. 26 interactions.
IntActiQ6NUM9. 1 interaction.
MINTiMINT-3039422.
STRINGi9606.ENSP00000295802.

Chemistry

DrugBankiDB00162. Vitamin A.

PTM databases

iPTMnetiQ6NUM9.
PhosphoSiteiQ6NUM9.
SwissPalmiQ6NUM9.

Polymorphism and mutation databases

BioMutaiRETSAT.
DMDMi90108452.

Proteomic databases

EPDiQ6NUM9.
MaxQBiQ6NUM9.
PaxDbiQ6NUM9.
PeptideAtlasiQ6NUM9.
PRIDEiQ6NUM9.

Protocols and materials databases

DNASUi54884.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295802; ENSP00000295802; ENSG00000042445. [Q6NUM9-1]
GeneIDi54884.
KEGGihsa:54884.
UCSCiuc002spd.4. human. [Q6NUM9-1]

Organism-specific databases

CTDi54884.
GeneCardsiRETSAT.
HGNCiHGNC:25991. RETSAT.
HPAiHPA007961.
HPA046513.
neXtProtiNX_Q6NUM9.
PharmGKBiPA145007867.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4254. Eukaryota.
COG1233. LUCA.
GeneTreeiENSGT00390000017613.
HOVERGENiHBG079484.
InParanoidiQ6NUM9.
KOiK09516.
OMAiFSMHALL.
OrthoDBiEOG091G04VP.
PhylomeDBiQ6NUM9.
TreeFamiTF328375.

Miscellaneous databases

ChiTaRSiRETSAT. human.
GeneWikiiRETSAT.
GenomeRNAii54884.
PROiQ6NUM9.

Gene expression databases

BgeeiENSG00000042445.
CleanExiHS_RETSAT.
ExpressionAtlasiQ6NUM9. baseline and differential.
GenevisibleiQ6NUM9. HS.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiRETST_HUMAN
AccessioniPrimary (citable) accession number: Q6NUM9
Secondary accession number(s): A6NIK3
, Q53R95, Q53SA9, Q6UX05, Q8N2H5, Q96FA4, Q9NXE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.