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Protein

Calcium-binding mitochondrial carrier protein SCaMC-1

Gene

SLC25A24

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent mitochondrial solute carrier. Mediates the reversible, electroneutral exchange of Mg-ATP or Mg-ADP against phosphate ions, catalyzing the net uptake or efflux of adenine nucleotides across the mitochondrial inner membrane. Nucleotide transport is inactive when cytosolic calcium levels are low, and is activated by an increase in cytosolic calcium levels. May play a role in protecting cells against oxidative stress-induced cell death, probably by promoting the formation of calcium-phosphate precipitates in the mitochondrial matrix, and thereby buffering calcium levels in the mitochondrial matrix.2 Publications

Kineticsi

  1. KM=0.97 mM for AMP1 Publication
  2. KM=0.3 mM for ADP1 Publication
  3. KM=0.33 mM for ATP1 Publication
  4. KM=0.2 mM for ATP-Mg1 Publication
  5. KM=1.64 mM for Pi1 Publication
  1. Vmax=337 µmol/min/g enzyme with AMP as substrate1 Publication
  2. Vmax=345 µmol/min/g enzyme with ADP as substrate1 Publication
  3. Vmax=320 µmol/min/g enzyme with ATP as substrate1 Publication
  4. Vmax=365 µmol/min/g enzyme with ATP-Mg as substrate1 Publication
  5. Vmax=380 µmol/min/g enzyme with Pi as substrate1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi32 – 431Add BLAST12
Calcium bindingi68 – 792Add BLAST12
Calcium bindingi99 – 1103Add BLAST12
Calcium bindingi135 – 1464Add BLAST12

GO - Molecular functioni

  • ADP transmembrane transporter activity Source: GO_Central
  • ATP transmembrane transporter activity Source: UniProtKB
  • calcium ion binding Source: UniProtKB

GO - Biological processi

  • ATP transport Source: UniProtKB
  • cellular response to calcium ion Source: UniProtKB
  • cellular response to oxidative stress Source: UniProtKB
  • mitochondrial transport Source: UniProtKB
  • regulation of cell death Source: UniProtKB

Keywordsi

Biological processTransport
LigandCalcium, Metal-binding

Protein family/group databases

TCDBi2.A.29.23.8. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding mitochondrial carrier protein SCaMC-1
Alternative name(s):
Mitochondrial ATP-Mg/Pi carrier protein 1
Mitochondrial Ca(2+)-dependent solute carrier protein 1
Small calcium-binding mitochondrial carrier protein 1
Solute carrier family 25 member 24
Gene namesi
Name:SLC25A24
Synonyms:APC1, MCSC1, SCAMC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:20662. SLC25A24.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 197Mitochondrial intermembraneSequence analysisAdd BLAST197
Transmembranei198 – 215Helical; Name=1Sequence analysisAdd BLAST18
Topological domaini216 – 252Mitochondrial matrixSequence analysisAdd BLAST37
Transmembranei253 – 272Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini273 – 295Mitochondrial intermembraneSequence analysisAdd BLAST23
Transmembranei296 – 309Helical; Name=3Sequence analysisAdd BLAST14
Topological domaini310 – 345Mitochondrial matrixSequence analysisAdd BLAST36
Transmembranei346 – 365Helical; Name=4Sequence analysisAdd BLAST20
Topological domaini366 – 388Mitochondrial intermembraneSequence analysisAdd BLAST23
Transmembranei389 – 406Helical; Name=5Sequence analysisAdd BLAST18
Topological domaini407 – 445Mitochondrial matrixSequence analysisAdd BLAST39
Transmembranei446 – 465Helical; Name=6Sequence analysisAdd BLAST20
Topological domaini466 – 477Mitochondrial intermembraneSequence analysisAdd BLAST12

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • mitochondrial inner membrane Source: GO_Central
  • mitochondrion Source: UniProtKB

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi29957.
OpenTargetsiENSG00000085491.
PharmGKBiPA134978257.

Polymorphism and mutation databases

BioMutaiSLC25A24.
DMDMi167016554.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003175941 – 477Calcium-binding mitochondrial carrier protein SCaMC-1Add BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei320N6-acetyllysine; alternateBy similarity1
Modified residuei320N6-succinyllysine; alternateBy similarity1
Modified residuei336N6-acetyllysineCombined sources1
Modified residuei437N6-acetyllysine; alternateCombined sources1
Modified residuei437N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ6NUK1.
MaxQBiQ6NUK1.
PaxDbiQ6NUK1.
PeptideAtlasiQ6NUK1.
PRIDEiQ6NUK1.
TopDownProteomicsiQ6NUK1-1. [Q6NUK1-1]
Q6NUK1-2. [Q6NUK1-2]

PTM databases

iPTMnetiQ6NUK1.
PhosphoSitePlusiQ6NUK1.
SwissPalmiQ6NUK1.

Expressioni

Tissue specificityi

Present in various cell lines (at protein level). Expressed in all tissues tested. Highly expressed in testis, expressed at intermediate level in small intestine and pancreas, and weakly expressed in kidney, spleen, liver, skeletal muscle and heart.2 Publications

Gene expression databases

BgeeiENSG00000085491.
CleanExiHS_SLC25A24.
ExpressionAtlasiQ6NUK1. baseline and differential.
GenevisibleiQ6NUK1. HS.

Organism-specific databases

HPAiHPA028519.
HPA063636.

Interactioni

Protein-protein interaction databases

BioGridi118993. 37 interactors.
IntActiQ6NUK1. 5 interactors.
MINTiMINT-4905805.
STRINGi9606.ENSP00000457733.

Structurei

Secondary structure

1477
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 31Combined sources8
Beta strandi36 – 39Combined sources4
Helixi41 – 49Combined sources9
Turni50 – 52Combined sources3
Helixi59 – 67Combined sources9
Beta strandi72 – 75Combined sources4
Helixi77 – 98Combined sources22
Beta strandi103 – 106Combined sources4
Helixi108 – 117Combined sources10
Helixi124 – 134Combined sources11
Beta strandi136 – 138Combined sources3
Beta strandi140 – 142Combined sources3
Helixi144 – 151Combined sources8
Helixi159 – 168Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4N5XX-ray2.10A1-193[»]
4ZCUX-ray2.10A/B/C14-174[»]
4ZCVX-ray2.80A/B/C/D14-174[»]
ProteinModelPortaliQ6NUK1.
SMRiQ6NUK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 54EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini55 – 88EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini86 – 121EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini122 – 157EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Repeati192 – 278Solcar 1Add BLAST87
Repeati286 – 371Solcar 2Add BLAST86
Repeati383 – 471Solcar 3Add BLAST89

Domaini

The N-terminal domain can bind calcium and regulates the ATP carrier activity of the transmembrane domain. The apo form of the N-terminal domain is intrinsically disordered and binds to the transmembrane domain, leading to inhibition of the ATP carrier activity. Calcium binding leads to a major conformation change and abolishes the interaction with the transmembrane domain and the inhibition of the ATP carrier activity (PubMed:24332718).1 Publication

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0036. Eukaryota.
ENOG410XQ4P. LUCA.
GeneTreeiENSGT00880000137904.
HOVERGENiHBG108464.
InParanoidiQ6NUK1.
KOiK14684.
OMAiMQAQAMV.
OrthoDBiEOG091G0C8S.
PhylomeDBiQ6NUK1.
TreeFamiTF313492.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002167. Graves_DC.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
PfamiView protein in Pfam
PF13499. EF-hand_7. 2 hits.
PF00153. Mito_carr. 3 hits.
PRINTSiPR00928. GRAVESDC.
PR00926. MITOCARRIER.
SMARTiView protein in SMART
SM00054. EFh. 3 hits.
SUPFAMiSSF103506. SSF103506. 1 hit.
SSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
PS50920. SOLCAR. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NUK1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRWLRDFVL PTAACQDAEQ PTRYETLFQA LDRNGDGVVD IGELQEGLRN
60 70 80 90 100
LGIPLGQDAE EKIFTTGDVN KDGKLDFEEF MKYLKDHEKK MKLAFKSLDK
110 120 130 140 150
NNDGKIEASE IVQSLQTLGL TISEQQAELI LQSIDVDGTM TVDWNEWRDY
160 170 180 190 200
FLFNPVTDIE EIIRFWKHST GIDIGDSLTI PDEFTEDEKK SGQWWRQLLA
210 220 230 240 250
GGIAGAVSRT STAPLDRLKI MMQVHGSKSD KMNIFGGFRQ MVKEGGIRSL
260 270 280 290 300
WRGNGTNVIK IAPETAVKFW AYEQYKKLLT EEGQKIGTFE RFISGSMAGA
310 320 330 340 350
TAQTFIYPME VMKTRLAVGK TGQYSGIYDC AKKILKHEGL GAFYKGYVPN
360 370 380 390 400
LLGIIPYAGI DLAVYELLKS YWLDNFAKDS VNPGVMVLLG CGALSSTCGQ
410 420 430 440 450
LASYPLALVR TRMQAQAMLE GSPQLNMVGL FRRIISKEGI PGLYRGITPN
460 470
FMKVLPAVGI SYVVYENMKQ TLGVTQK
Length:477
Mass (Da):53,354
Last modified:February 5, 2008 - v2
Checksum:iF533D47B2457123F
GO
Isoform 2 (identifier: Q6NUK1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MLRWLRDFVL...GIPLGQDAEE → MDSLYGDLFW...GAIQSLEEAK

Show »
Length:458
Mass (Da):51,354
Checksum:i8C70A5782DE8BF3D
GO

Sequence cautioni

The sequence AAF28888 differs from that shown. Reason: Frameshift at positions 418 and 464.Curated
The sequence CAI13622 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI14512 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75L → P in AAH68561 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0310661 – 61MLRWL…QDAEE → MDSLYGDLFWYLDYNKDGTL DIFELQEGLEDVGAIQSLEE AK in isoform 2. 2 PublicationsAdd BLAST61

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ619987 mRNA. Translation: CAF04493.1.
AJ619961 mRNA. Translation: CAF04058.1.
AK292567 mRNA. Translation: BAF85256.1.
AK316304 mRNA. Translation: BAH14675.1.
AL390036, AL359258 Genomic DNA. Translation: CAI13622.1. Sequence problems.
AL390036, AL359258 Genomic DNA. Translation: CAI13623.1.
AL359258, AL390036 Genomic DNA. Translation: CAI14512.1. Sequence problems.
AL359258, AL390036 Genomic DNA. Translation: CAI14513.1.
CH471156 Genomic DNA. Translation: EAW51254.1.
CH471156 Genomic DNA. Translation: EAW51255.1.
BC014519 mRNA. Translation: AAH14519.1.
BC068561 mRNA. Translation: AAH68561.1.
AF123303 mRNA. Translation: AAF28888.1. Frameshift.
CCDSiCCDS41361.1. [Q6NUK1-1]
CCDS786.1. [Q6NUK1-2]
RefSeqiNP_037518.3. NM_013386.4. [Q6NUK1-1]
NP_998816.1. NM_213651.2. [Q6NUK1-2]
UniGeneiHs.656870.

Genome annotation databases

EnsembliENST00000370041; ENSP00000359058; ENSG00000085491. [Q6NUK1-2]
ENST00000565488; ENSP00000457733; ENSG00000085491. [Q6NUK1-1]
ENST00000639032; ENSP00000492810; ENSG00000284468. [Q6NUK1-2]
ENST00000640416; ENSP00000491572; ENSG00000284468. [Q6NUK1-1]
GeneIDi29957.
KEGGihsa:29957.
UCSCiuc001dvm.5. human. [Q6NUK1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSCMC1_HUMAN
AccessioniPrimary (citable) accession number: Q6NUK1
Secondary accession number(s): B7ZAI9
, Q5T331, Q5T485, Q6PJJ9, Q705K4, Q9P129
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: August 30, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families