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Q6NUI2 (GPAT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate acyltransferase 2, mitochondrial

Short name=GPAT-2
EC=2.3.1.15
Alternative name(s):
xGPAT1
Gene names
Name:GPAT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length795 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis By similarity.

Catalytic activity

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

Pathway

Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.

Subcellular location

Mitochondrion outer membrane; Multi-pass membrane protein By similarity.

Domain

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.

Sequence similarities

Belongs to the GPAT/DAPAT family.

Sequence caution

The sequence AAH68596.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAD18392.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6NUI2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6NUI2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     633-671: TPGSRPACDT...SDSDDFGEAD → SWATQSSCSS...PSVLSGPSET
     672-795: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q6NUI2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     476-476: K → KGVFLSQ
Note: No experimental confirmation available.
Isoform 4 (identifier: Q6NUI2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-216: Missing.
     274-344: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 795795Glycerol-3-phosphate acyltransferase 2, mitochondrial
PRO_0000325853

Regions

Topological domain1 – 305305Cytoplasmic Potential
Transmembrane306 – 33227Helical; Potential
Topological domain333 – 449117Mitochondrial intermembrane Potential
Transmembrane450 – 47223Helical; Potential
Topological domain473 – 795323Cytoplasmic Potential
Region180 – 331152Acyltransferase
Motif205 – 2106HXXXXD motif

Natural variations

Alternative sequence1 – 216216Missing in isoform 4.
VSP_032450
Alternative sequence274 – 34471Missing in isoform 4.
VSP_032451
Alternative sequence4761K → KGVFLSQ in isoform 3.
VSP_032452
Alternative sequence633 – 67139TPGSR…FGEAD → SWATQSSCSSSCRPPPRKKG SSSVRTQSSPSVLSGPSET in isoform 2.
VSP_032453
Alternative sequence672 – 795124Missing in isoform 2.
VSP_032454

Experimental info

Sequence conflict3361P → Q in AAH68596. Ref.2
Sequence conflict6891F → L in BAC85508. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: EE8E387A87A9EE21

FASTA79587,835
        10         20         30         40         50         60 
MATMLEGRCQ TQPRSSPSGR EASLWSSGFG MKLEAVTPFL GKYRPFVGRC CQTCTPKSWE 

        70         80         90        100        110        120 
SLFHRSITDL GFCNVILVKE ENTRFRGWLV RRLCYFLWSL EQHIPPCQDV PQKIMESTGV 

       130        140        150        160        170        180 
QNLLSGRVPG GTGEGQVPDL VKKEVQRILG HIQAPPRPFL VRLFSWALLR FLNCLFLNVQ 

       190        200        210        220        230        240 
LHKGQMKMVQ KAAQAGLPLV LLSTHKTLLD GILLPFMLLS QGLGVLRVAW DSRACSPALR 

       250        260        270        280        290        300 
ALLRKLGGLF LPPEASLSLD SSEGLLARAV VQAVIEQLLV SGQPLLIFLE EPPGALGPRL 

       310        320        330        340        350        360 
SALGQAWVGF VVQAVQVGIV PDALLVPVAV TYDLVPDAPC DIDHASAPLG LWTGALAVLR 

       370        380        390        400        410        420 
SLWSRWGCSH RICSRVHLAQ PFSLQEYIVS ARSCWGGRQT LEQLLQPIVL GQCTAVPDTE 

       430        440        450        460        470        480 
KEQEWTPITG PLLALKEEDQ LLVRRLSCHV LSASVGSSAV MSTAIMATLL LFKHQKLLGE 

       490        500        510        520        530        540 
FSWLTEEILL RGFDVGFSGQ LRSLLQHSLS LLRAHVALLR IRQGDLLVVP QPGPGLTHLA 

       550        560        570        580        590        600 
QLSAELLPVF LSEAVGACAV RGLLAGRVPP QGPWELQGIL LLSQNELYRQ ILLLMHLLPQ 

       610        620        630        640        650        660 
DLLLLKPCQS SYCYCQEVLD RLIQCGLLVA EETPGSRPAC DTGRQRLSRK LLWKPSGDFT 

       670        680        690        700        710        720 
DSDSDDFGEA DGRYFRLSQQ SHCPDFFLFL CRLLSPLLKA FAQAAAFLRQ GQLPDTELGY 

       730        740        750        760        770        780 
TEQLFQFLQA TAQEEGIFEC ADPKLAISAV WTFRDLGVLQ QTPSPAGPRL HLSPTFASLD 

       790 
NQEKLEQFIR QFICS 

« Hide

Isoform 2 [UniParc].

Checksum: F44C1200F3AA60A6
Show »

FASTA67173,521
Isoform 3 [UniParc].

Checksum: 5E29D4F62C9292B0
Show »

FASTA80188,467
Isoform 4 [UniParc].

Checksum: D405B71CFA1125F1
Show »

FASTA50856,052

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 165-795 (ISOFORM 3).
Tissue: Brain, Heart and Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK122734 mRNA. Translation: BAC85508.1.
AK131194 mRNA. Translation: BAD18390.1.
AK131197 mRNA. Translation: BAD18392.1. Different initiation.
BC068596 mRNA. Translation: AAH68596.1. Different initiation.
CCDSCCDS42714.1. [Q6NUI2-1]
RefSeqNP_997211.2. NM_207328.2. [Q6NUI2-1]
XP_005263941.1. XM_005263884.1. [Q6NUI2-4]
XP_005263945.1. XM_005263888.1. [Q6NUI2-3]
XP_006712378.1. XM_006712315.1. [Q6NUI2-3]
UniGeneHs.348629.
Hs.679801.

3D structure databases

ProteinModelPortalQ6NUI2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127324. 1 interaction.
STRING9606.ENSP00000352547.

PTM databases

PhosphoSiteQ6NUI2.

Polymorphism databases

DMDM172046129.

Proteomic databases

PaxDbQ6NUI2.
PRIDEQ6NUI2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000359548; ENSP00000352547; ENSG00000186281. [Q6NUI2-1]
ENST00000377137; ENSP00000366341; ENSG00000186281. [Q6NUI2-3]
ENST00000434632; ENSP00000389395; ENSG00000186281. [Q6NUI2-1]
GeneID150763.
KEGGhsa:150763.
UCSCuc002svd.3. human. [Q6NUI2-4]
uc002svf.3. human. [Q6NUI2-1]
uc002svh.3. human. [Q6NUI2-3]

Organism-specific databases

CTD150763.
GeneCardsGC02M096687.
HGNCHGNC:27168. GPAT2.
HPAHPA036841.
neXtProtNX_Q6NUI2.
PharmGKBPA165696677.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG134494.
KOK00629.
OMACVRVHLA.
OrthoDBEOG74R1PZ.
PhylomeDBQ6NUI2.
TreeFamTF313360.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
UniPathwayUPA00557; UER00612.

Gene expression databases

BgeeQ6NUI2.
GenevestigatorQ6NUI2.

Family and domain databases

InterProIPR022284. GPAT/DHAPAT.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERPTHR12563. PTHR12563. 1 hit.
SMARTSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi150763.
NextBio86529.
PROQ6NUI2.

Entry information

Entry nameGPAT2_HUMAN
AccessionPrimary (citable) accession number: Q6NUI2
Secondary accession number(s): Q6P2E4 expand/collapse secondary AC list , Q6ZNI3, Q6ZNI5, Q6ZWJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: July 9, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM