Reviewed, UniProtKB/Swiss-Prot Q6NUI2 (GPAT2_HUMAN)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase 2, mitochondrial
    EC=2.3.1.15
Alternative name(s):
    xGPAT1

Gene names

Name:

GPAT2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis By similarity.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Mitochondrion outer membrane; Multi-pass membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6NUI2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6NUI2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     633-671: TPGSRPACDT...SDSDDFGEAD → SWATQSSCSS...PSVLSGPSET
     672-795: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6NUI2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     476-476: K → KGVFLSQ

Note: No experimental confirmation available.

Isoform 4 (identifier: Q6NUI2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-216: Missing.
     274-344: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 795	795	Glycerol-3-phosphate acyltransferase 2, mitochondrial		PRO_0000325853
Regions
	Transmembrane	159 – 179	21	Potential
	Transmembrane	452 – 472	21	Potential
	Region	180 – 331	152	Acyltransferase
	Motif	205 – 210	6	HXXXXD motif
Natural variations
	Alternative sequence	1 – 216	216	Missing in isoform 4.		VSP_032450
	Alternative sequence	274 – 344	71	Missing in isoform 4.		VSP_032451
	Alternative sequence	476	1	K → KGVFLSQ in isoform 3.		VSP_032452
	Alternative sequence	633 – 671	39	TPGSR…FGEAD → SWATQSSCSSSCRPPPRKKG SSSVRTQSSPSVLSGPSET in isoform 2.		VSP_032453
	Alternative sequence	672 – 795	124	Missing in isoform 2.		VSP_032454
Experimental info
	Sequence conflict	336	1	P → Q in AAH68596. Ref.2
	Sequence conflict	689	1	F → L in BAC85508. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: EE8E387A87A9EE21	FASTA	795	87,835
	10 20 30 40 50 60 MATMLEGRCQ TQPRSSPSGR EASLWSSGFG MKLEAVTPFL GKYRPFVGRC CQTCTPKSWE 70 80 90 100 110 120 SLFHRSITDL GFCNVILVKE ENTRFRGWLV RRLCYFLWSL EQHIPPCQDV PQKIMESTGV 130 140 150 160 170 180 QNLLSGRVPG GTGEGQVPDL VKKEVQRILG HIQAPPRPFL VRLFSWALLR FLNCLFLNVQ 190 200 210 220 230 240 LHKGQMKMVQ KAAQAGLPLV LLSTHKTLLD GILLPFMLLS QGLGVLRVAW DSRACSPALR 250 260 270 280 290 300 ALLRKLGGLF LPPEASLSLD SSEGLLARAV VQAVIEQLLV SGQPLLIFLE EPPGALGPRL 310 320 330 340 350 360 SALGQAWVGF VVQAVQVGIV PDALLVPVAV TYDLVPDAPC DIDHASAPLG LWTGALAVLR 370 380 390 400 410 420 SLWSRWGCSH RICSRVHLAQ PFSLQEYIVS ARSCWGGRQT LEQLLQPIVL GQCTAVPDTE 430 440 450 460 470 480 KEQEWTPITG PLLALKEEDQ LLVRRLSCHV LSASVGSSAV MSTAIMATLL LFKHQKLLGE 490 500 510 520 530 540 FSWLTEEILL RGFDVGFSGQ LRSLLQHSLS LLRAHVALLR IRQGDLLVVP QPGPGLTHLA 550 560 570 580 590 600 QLSAELLPVF LSEAVGACAV RGLLAGRVPP QGPWELQGIL LLSQNELYRQ ILLLMHLLPQ 610 620 630 640 650 660 DLLLLKPCQS SYCYCQEVLD RLIQCGLLVA EETPGSRPAC DTGRQRLSRK LLWKPSGDFT 670 680 690 700 710 720 DSDSDDFGEA DGRYFRLSQQ SHCPDFFLFL CRLLSPLLKA FAQAAAFLRQ GQLPDTELGY 730 740 750 760 770 780 TEQLFQFLQA TAQEEGIFEC ADPKLAISAV WTFRDLGVLQ QTPSPAGPRL HLSPTFASLD 790 NQEKLEQFIR QFICS
	Isoform 2. Checksum: F44C1200F3AA60A6	FASTA	671	73,521
	10 20 30 40 50 60 MATMLEGRCQ TQPRSSPSGR EASLWSSGFG MKLEAVTPFL GKYRPFVGRC CQTCTPKSWE 70 80 90 100 110 120 SLFHRSITDL GFCNVILVKE ENTRFRGWLV RRLCYFLWSL EQHIPPCQDV PQKIMESTGV 130 140 150 160 170 180 QNLLSGRVPG GTGEGQVPDL VKKEVQRILG HIQAPPRPFL VRLFSWALLR FLNCLFLNVQ 190 200 210 220 230 240 LHKGQMKMVQ KAAQAGLPLV LLSTHKTLLD GILLPFMLLS QGLGVLRVAW DSRACSPALR 250 260 270 280 290 300 ALLRKLGGLF LPPEASLSLD SSEGLLARAV VQAVIEQLLV SGQPLLIFLE EPPGALGPRL 310 320 330 340 350 360 SALGQAWVGF VVQAVQVGIV PDALLVPVAV TYDLVPDAPC DIDHASAPLG LWTGALAVLR 370 380 390 400 410 420 SLWSRWGCSH RICSRVHLAQ PFSLQEYIVS ARSCWGGRQT LEQLLQPIVL GQCTAVPDTE 430 440 450 460 470 480 KEQEWTPITG PLLALKEEDQ LLVRRLSCHV LSASVGSSAV MSTAIMATLL LFKHQKLLGE 490 500 510 520 530 540 FSWLTEEILL RGFDVGFSGQ LRSLLQHSLS LLRAHVALLR IRQGDLLVVP QPGPGLTHLA 550 560 570 580 590 600 QLSAELLPVF LSEAVGACAV RGLLAGRVPP QGPWELQGIL LLSQNELYRQ ILLLMHLLPQ 610 620 630 640 650 660 DLLLLKPCQS SYCYCQEVLD RLIQCGLLVA EESWATQSSC SSSCRPPPRK KGSSSVRTQS 670 SPSVLSGPSE T
	Isoform 3. Checksum: 5E29D4F62C9292B0	FASTA	801	88,467
	10 20 30 40 50 60 MATMLEGRCQ TQPRSSPSGR EASLWSSGFG MKLEAVTPFL GKYRPFVGRC CQTCTPKSWE 70 80 90 100 110 120 SLFHRSITDL GFCNVILVKE ENTRFRGWLV RRLCYFLWSL EQHIPPCQDV PQKIMESTGV 130 140 150 160 170 180 QNLLSGRVPG GTGEGQVPDL VKKEVQRILG HIQAPPRPFL VRLFSWALLR FLNCLFLNVQ 190 200 210 220 230 240 LHKGQMKMVQ KAAQAGLPLV LLSTHKTLLD GILLPFMLLS QGLGVLRVAW DSRACSPALR 250 260 270 280 290 300 ALLRKLGGLF LPPEASLSLD SSEGLLARAV VQAVIEQLLV SGQPLLIFLE EPPGALGPRL 310 320 330 340 350 360 SALGQAWVGF VVQAVQVGIV PDALLVPVAV TYDLVPDAPC DIDHASAPLG LWTGALAVLR 370 380 390 400 410 420 SLWSRWGCSH RICSRVHLAQ PFSLQEYIVS ARSCWGGRQT LEQLLQPIVL GQCTAVPDTE 430 440 450 460 470 480 KEQEWTPITG PLLALKEEDQ LLVRRLSCHV LSASVGSSAV MSTAIMATLL LFKHQKGVFL 490 500 510 520 530 540 SQLLGEFSWL TEEILLRGFD VGFSGQLRSL LQHSLSLLRA HVALLRIRQG DLLVVPQPGP 550 560 570 580 590 600 GLTHLAQLSA ELLPVFLSEA VGACAVRGLL AGRVPPQGPW ELQGILLLSQ NELYRQILLL 610 620 630 640 650 660 MHLLPQDLLL LKPCQSSYCY CQEVLDRLIQ CGLLVAEETP GSRPACDTGR QRLSRKLLWK 670 680 690 700 710 720 PSGDFTDSDS DDFGEADGRY FRLSQQSHCP DFFLFLCRLL SPLLKAFAQA AAFLRQGQLP 730 740 750 760 770 780 DTELGYTEQL FQFLQATAQE EGIFECADPK LAISAVWTFR DLGVLQQTPS PAGPRLHLSP 790 800 TFASLDNQEK LEQFIRQFIC S
	Isoform 4. Checksum: D405B71CFA1125F1	FASTA	508	56,052
	10 20 30 40 50 60 MLLSQGLGVL RVAWDSRACS PALRALLRKL GGLFLPPEAS LSLDSSEGLL ARAVVQAASA 70 80 90 100 110 120 PLGLWTGALA VLRSLWSRWG CSHRICSRVH LAQPFSLQEY IVSARSCWGG RQTLEQLLQP 130 140 150 160 170 180 IVLGQCTAVP DTEKEQEWTP ITGPLLALKE EDQLLVRRLS CHVLSASVGS SAVMSTAIMA 190 200 210 220 230 240 TLLLFKHQKL LGEFSWLTEE ILLRGFDVGF SGQLRSLLQH SLSLLRAHVA LLRIRQGDLL 250 260 270 280 290 300 VVPQPGPGLT HLAQLSAELL PVFLSEAVGA CAVRGLLAGR VPPQGPWELQ GILLLSQNEL 310 320 330 340 350 360 YRQILLLMHL LPQDLLLLKP CQSSYCYCQE VLDRLIQCGL LVAEETPGSR PACDTGRQRL 370 380 390 400 410 420 SRKLLWKPSG DFTDSDSDDF GEADGRYFRL SQQSHCPDFF LFLCRLLSPL LKAFAQAAAF 430 440 450 460 470 480 LRQGQLPDTE LGYTEQLFQF LQATAQEEGI FECADPKLAI SAVWTFRDLG VLQQTPSPAG 490 500 PRLHLSPTFA SLDNQEKLEQ FIRQFICS

References

[1]

"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 165-795 (ISOFORM 3). Tissue: Brain, Heart and Testis.

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK122734 mRNA. Translation: BAC85508.1. AK131194 mRNA. Translation: BAD18390.1. AK131197 mRNA. Translation: BAD18392.1. Different initiation. BC068596 mRNA. Translation: AAH68596.1. Different initiation.
IPI	IPI00419675. IPI00442339. IPI00744938. IPI00888543.
RefSeq	NP_997211.2.
UniGene	Hs.590914 Hs.679801
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q6NUI2.
Genome annotation databases
Ensembl	ENST00000335448; ENSP00000335216; ENSG00000186281; Homo sapiens. [Genome view] ENST00000359548; ENSP00000352547; ENSG00000186281; Homo sapiens. [Genome view] ENST00000377137; ENSP00000366341; ENSG00000186281; Homo sapiens. [Genome view] ENST00000398024; ENSP00000381106; ENSG00000186281; Homo sapiens. [Genome view] ENST00000434632; ENSP00000389395; ENSG00000186281; Homo sapiens. [Genome view] ENST00000439254; ENSP00000401334; ENSG00000186281; Homo sapiens. [Genome view] ENST00000453542; ENSP00000393770; ENSG00000186281; Homo sapiens. [Genome view]
GeneID	150763.
KEGG	hsa:150763.
UCSC	uc002svd.1. human. uc002sve.1. human. uc002svf.1. human. uc002svh.1. human.
Organism-specific databases
CTD	150763.
GeneCards	GC02M096109. GC02P097112.
HGNC	HGNC:27168. GPAT2.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q6NUI2.
Enzyme and pathway databases
BRENDA	2.3.1.15. 247.
Gene expression databases
ArrayExpress	Q6NUI2.
Genevestigator	Q6NUI2.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	86529.

Entry information

Entry name

GPAT2_HUMAN

Accession

Primary (citable) accession number: Q6NUI2
Secondary accession number(s): Q6P2E4 , Q6ZNI3, Q6ZNI5, Q6ZWJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	November 3, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.