ID   TPX2A_XENLA             Reviewed;         716 AA.
AC   Q6NUF4; Q9I8N0;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   19-OCT-2011, entry version 37.
DE   RecName: Full=Targeting protein for Xklp2-A;
GN   Name=tpx2-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Amphibia; Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae;
OC   Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION,
RP   SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND PHOSPHORYLATION.
RC   TISSUE=Egg;
RX   MEDLINE=20330161; PubMed=10871281; DOI=10.1083/jcb.149.7.1405;
RA   Wittmann T., Wilm M., Karsenti E., Vernos I.;
RT   "TPX2, a novel Xenopus MAP involved in spindle pole organization.";
RL   J. Cell Biol. 149:1405-1418(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   INTERACTION WITH KIF15, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RX   PubMed=9813089; DOI=10.1083/jcb.143.3.673;
RA   Wittmann T., Boleti H., Antony C., Karsenti E., Vernos I.;
RT   "Localization of the kinesin-like protein Xklp2 to spindle poles
RT   requires a leucine zipper, a microtubule-associated protein, and
RT   dynein.";
RL   J. Cell Biol. 143:673-685(1998).
RN   [4]
RP   FUNCTION, PHOSPHORYLATION AT SER-204, MUTAGENESIS OF SER-204, AND
RP   INTERACTION WITH PLK1.
RX   PubMed=19556869;
RA   Eckerdt F., Pascreau G., Phistry M., Lewellyn A.L.,
RA   DePaoli-Roach A.A., Maller J.L.;
RT   "Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A.";
RL   Cell Cycle 8:2413-2419(2009).
CC   -!- FUNCTION: Spindle assembly factor. Required for normal assembly of
CC       mitotic spindles. Mediates the binding kif15 and aurka to spindle
CC       microtubules. Required for targeting kif15 to microtubule minus
CC       ends. Activates aurka by promoting its autophosphorylation and
CC       protects the phosphorylated residue against dephosphorylation (By
CC       similarity).
CC   -!- SUBUNIT: Associates with microtubules. Interacts with aurka and
CC       plk1. Interacts with kif15.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, spindle.
CC       Cytoplasm, cytoskeleton, spindle pole. Note=Localizes during
CC       metaphase on spindle microtubules, with a strong enrichment at
CC       spindle poles. Localizes to the minus ends of spindle pole and
CC       aster microtubules in a dynein- and dynactin-dependent manner.
CC   -!- DEVELOPMENTAL STAGE: Detected in mitotic eggs (at protein level).
CC   -!- PTM: Phosphorylated during mitosis. Hyperphosphorylated upon
CC       assembly of microtubules.
CC   -!- SIMILARITY: Belongs to the TPX2 family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AF244546; AAF81694.1; -; mRNA.
DR   EMBL; BC068637; AAH68637.1; -; mRNA.
DR   RefSeq; NP_001082006.1; NM_001088537.1.
DR   UniGene; Xl.658; -.
DR   PDB; 3KND; X-ray; 2.15 A; B=270-350.
DR   PDBsum; 3KND; -.
DR   ProteinModelPortal; Q6NUF4; -.
DR   SMR; Q6NUF4; 7-39.
DR   IntAct; Q6NUF4; 4.
DR   GeneID; 398174; -.
DR   KEGG; xla:398174; -.
DR   CTD; 398174; -.
DR   Xenbase; XB-GENE-6251919; tpx2.
DR   HOVERGEN; HBG057334; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007067; P:mitosis; IEA:UniProtKB-KW.
DR   InterPro; IPR015128; Aurora-A-bd.
DR   InterPro; IPR022021; TPX2_importin.
DR   InterPro; IPR009675; Xklp2_targeting_prot.
DR   Pfam; PF09041; Aurora-A_bind; 1.
DR   Pfam; PF06886; TPX2; 1.
DR   Pfam; PF12214; TPX2_importin; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell cycle; Cell division; Cytoplasm; Cytoskeleton;
KW   Direct protein sequencing; Microtubule; Mitosis; Nucleus;
KW   Phosphoprotein.
FT   CHAIN         1    716       Targeting protein for Xklp2-A.
FT                                /FTId=PRO_0000393114.
FT   MOD_RES     204    204       Phosphoserine; by plk1.
FT   MUTAGEN     204    204       S->A: Decreases activation of aurka.
FT   MUTAGEN     204    204       S->D: Increases activation of aurka.
FT   CONFLICT     61     61       E -> Q (in Ref. 1; AAF81694).
FT   CONFLICT    473    473       Missing (in Ref. 1; AAF81694).
SQ   SEQUENCE   716 AA;  82512 MW;  F402B114123E839C CRC64;
     MEDTQDTYSY DAPSIFNFSS FHEDHNADSW FDQVTNAENI PPDQRRLSET SVNTEQNSKV
     EPVQTTPSKD DVSNSATHVC DVKSQSKRSS RRMSKKHRQK LLVKMKDTHL EKETAPPEYP
     PCKKLKGSSS KGRHAPVIKS QSTSSHHSMT SPKPKAQLTM PSTPTVLKRR NVLVKAKNSE
     EQELEKMQEL QKEMLENLKK NEHSMKVAIT GAGQPVKTFI PVTKPVDFHF KTDDRLKRTA
     NQPEGDGYKA VDFASELRKH PPSPVQVTKG GHTVPKPFNL SKGKRKHEEA SDYVSTAEQV
     IAFYKRTPAR YHLRSRQREM EGPSPVKMIK TKLTNPKTPL LQTKGRHRPV TCKSAAELEA
     EELEMINQYK FKAQELDTRI LEGGPVLLKK PLVKEPTKAI GFDLEIEKRI QQREKKEEIE
     EETFTFHSRP CPSKMLTDVV GVPLKKLLPV TVPQSPAFAL KNRVRIPAQE EKQEEMVPVI
     KATRMPHYGV PFKPKLVEQR QVDVCPFSFC DRDKERQLQK EKRLDELRKD EVPKFKAQPL
     PQFDNIRLPE KKVKMPTQQE PFDLEIEKRG ASKLQRWQQQ IQEELKQQKE MVVFKARPNT
     VVHQEPFVPK KENRSLTESL SGSIVQEGFE LATAKRAKER QEFDKCLAET EAQKSLLEEE
     IRKRREEEEK EEISQLRQEL VHKAKPIRKY RAVEVKASDV PLTVPRSPNF SDRFKC
//