Q6NUF4 (TPX2A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Targeting protein for Xklp2-A

Gene names

Name:

tpx2-a

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Spindle assembly factor. Required for normal assembly of mitotic spindles. Mediates the binding kif15 and aurka to spindle microtubules. Required for targeting kif15 to microtubule minus ends. Activates aurka by promoting its autophosphorylation and protects the phosphorylated residue against dephosphorylation By similarity. Ref.1 Ref.4
Subunit structure	Associates with microtubules. Interacts with aurka and plk1. Interacts with kif15. Ref.3 Ref.4
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Note: Localizes during metaphase on spindle microtubules, with a strong enrichment at spindle poles. Localizes to the minus ends of spindle pole and aster microtubules in a dynein- and dynactin-dependent manner. Ref.1 Ref.3
Developmental stage	Detected in mitotic eggs (at protein level). Ref.3
Post-translational modification	Phosphorylated during mitosis. Hyperphosphorylated upon assembly of microtubules. Ref.1 Ref.4
Sequence similarities	Belongs to the TPX2 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle pole Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 716	716	Targeting protein for Xklp2-A		PRO_0000393114
Amino acid modifications
	Modified residue	204	1	Phosphoserine; by plk1 Ref.4
Experimental info
	Mutagenesis	204	1	S → A: Decreases activation of aurka. Ref.4
	Mutagenesis	204	1	S → D: Increases activation of aurka. Ref.4
	Sequence conflict	61	1	E → Q in AAF81694. Ref.1
	Sequence conflict	473	1	Missing in AAF81694. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6NUF4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: F402B114123E839C	FASTA	716	82,512
	10 20 30 40 50 60 MEDTQDTYSY DAPSIFNFSS FHEDHNADSW FDQVTNAENI PPDQRRLSET SVNTEQNSKV 70 80 90 100 110 120 EPVQTTPSKD DVSNSATHVC DVKSQSKRSS RRMSKKHRQK LLVKMKDTHL EKETAPPEYP 130 140 150 160 170 180 PCKKLKGSSS KGRHAPVIKS QSTSSHHSMT SPKPKAQLTM PSTPTVLKRR NVLVKAKNSE 190 200 210 220 230 240 EQELEKMQEL QKEMLENLKK NEHSMKVAIT GAGQPVKTFI PVTKPVDFHF KTDDRLKRTA 250 260 270 280 290 300 NQPEGDGYKA VDFASELRKH PPSPVQVTKG GHTVPKPFNL SKGKRKHEEA SDYVSTAEQV 310 320 330 340 350 360 IAFYKRTPAR YHLRSRQREM EGPSPVKMIK TKLTNPKTPL LQTKGRHRPV TCKSAAELEA 370 380 390 400 410 420 EELEMINQYK FKAQELDTRI LEGGPVLLKK PLVKEPTKAI GFDLEIEKRI QQREKKEEIE 430 440 450 460 470 480 EETFTFHSRP CPSKMLTDVV GVPLKKLLPV TVPQSPAFAL KNRVRIPAQE EKQEEMVPVI 490 500 510 520 530 540 KATRMPHYGV PFKPKLVEQR QVDVCPFSFC DRDKERQLQK EKRLDELRKD EVPKFKAQPL 550 560 570 580 590 600 PQFDNIRLPE KKVKMPTQQE PFDLEIEKRG ASKLQRWQQQ IQEELKQQKE MVVFKARPNT 610 620 630 640 650 660 VVHQEPFVPK KENRSLTESL SGSIVQEGFE LATAKRAKER QEFDKCLAET EAQKSLLEEE 670 680 690 700 710 IRKRREEEEK EEISQLRQEL VHKAKPIRKY RAVEVKASDV PLTVPRSPNF SDRFKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TPX2, a novel Xenopus MAP involved in spindle pole organization." Wittmann T., Wilm M., Karsenti E., Vernos I. J. Cell Biol. 149:1405-1418(2000) [PubMed: 10871281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, PHOSPHORYLATION. Tissue: Egg.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	"Localization of the kinesin-like protein Xklp2 to spindle poles requires a leucine zipper, a microtubule-associated protein, and dynein." Wittmann T., Boleti H., Antony C., Karsenti E., Vernos I. J. Cell Biol. 143:673-685(1998) [PubMed: 9813089] [Abstract] Cited for: INTERACTION WITH KIF15, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
[4]	"Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A." Eckerdt F., Pascreau G., Phistry M., Lewellyn A.L., DePaoli-Roach A.A., Maller J.L. Cell Cycle 8:2413-2419(2009) [PubMed: 19556869] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT SER-204, MUTAGENESIS OF SER-204, INTERACTION WITH PLK1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF244546 mRNA. Translation: AAF81694.1.
BC068637 mRNA. Translation: AAH68637.1.

RefSeq

NP_001082006.1. NM_001088537.1.

UniGene

Xl.658.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3KND	X-ray	2.15	B	270-350	[»]

ProteinModelPortal

Q6NUF4.

SMR

Q6NUF4. Positions 7-39.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q6NUF4. 4 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

398174.

KEGG

xla:398174.

Organism-specific databases

CTD

398174.

Xenbase

XB-GENE-6251919. tpx2.

Phylogenomic databases

HOVERGEN

HBG057334.

Family and domain databases

InterPro

IPR015128. Aurora-A-bd.
IPR022021. TPX2_importin.
IPR009675. Xklp2_targeting_prot.
[Graphical view]

Pfam

PF09041. Aurora-A_bind. 1 hit.
PF06886. TPX2. 1 hit.
PF12214. TPX2_importin. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TPX2A_XENLA

Accession

Primary (citable) accession number: Q6NUF4
Secondary accession number(s): Q9I8N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	July 5, 2004
Last modified:	October 19, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program