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Protein

Methylosome protein 50

Gene

wdr77

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic component of the 20S prmt5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. Required for normal prmt5 methyltransferase activity.2 Publications

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome protein 50
Short name:
MEP-50
Alternative name(s):
WD repeat-containing protein 77
Gene namesi
Name:wdr77
Synonyms:mep50
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-972901. wdr77.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • histone methyltransferase complex Source: UniProtKB
  • methylosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Methylosome protein 50PRO_0000422973Add
BLAST

Proteomic databases

PRIDEiQ6NUD0.

Expressioni

Tissue specificityi

Detected in egg (at protein level).1 Publication

Interactioni

Subunit structurei

Heterotetramer; dimer of heterodimer with prmt5. Interacts with histone h2a and h4 and with nucleoplasmin.2 Publications

Protein-protein interaction databases

BioGridi101086. 2 interactions.

Structurei

Secondary structure

1
333
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi19 – 268Combined sources
Beta strandi28 – 303Combined sources
Beta strandi32 – 376Combined sources
Beta strandi39 – 435Combined sources
Beta strandi46 – 538Combined sources
Helixi60 – 623Combined sources
Beta strandi64 – 685Combined sources
Beta strandi73 – 797Combined sources
Turni80 – 823Combined sources
Beta strandi83 – 886Combined sources
Beta strandi93 – 953Combined sources
Beta strandi97 – 993Combined sources
Turni100 – 1034Combined sources
Beta strandi109 – 1113Combined sources
Beta strandi118 – 1236Combined sources
Beta strandi125 – 13410Combined sources
Beta strandi139 – 1435Combined sources
Turni144 – 1474Combined sources
Beta strandi148 – 1536Combined sources
Beta strandi160 – 1656Combined sources
Beta strandi173 – 1775Combined sources
Beta strandi182 – 1843Combined sources
Beta strandi189 – 1913Combined sources
Beta strandi205 – 2106Combined sources
Beta strandi217 – 2259Combined sources
Beta strandi227 – 2337Combined sources
Helixi235 – 2373Combined sources
Beta strandi239 – 2413Combined sources
Beta strandi248 – 2536Combined sources
Beta strandi255 – 2584Combined sources
Beta strandi261 – 2655Combined sources
Beta strandi270 – 2734Combined sources
Beta strandi279 – 2835Combined sources
Beta strandi290 – 2956Combined sources
Beta strandi297 – 2993Combined sources
Beta strandi302 – 3076Combined sources
Beta strandi312 – 3165Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4G56X-ray2.95B/D2-333[»]
ProteinModelPortaliQ6NUD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati16 – 5944WD 1Add
BLAST
Repeati68 – 10639WD 2Add
BLAST
Repeati113 – 15240WD 3Add
BLAST
Repeati155 – 19541WD 4Add
BLAST
Repeati199 – 24042WD 5Add
BLAST
Repeati243 – 28341WD 6Add
BLAST
Repeati285 – 32844WD 7Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOVERGENiHBG052458.
KOiK13221.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NUD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKGSAWGRP VTAPACMEVQ IGAVRYRRDG ALLLAASSLS SRTWGGSIWV
60 70 80 90 100
FKDPEGAPNE SLCTAGVQTE AGVTDVAWVS EKGILVASDS GAVELWEILE
110 120 130 140 150
KESLLVNKFA KYEHDDIVKT LSVFSDGTQA VSGGKDFSVK VWDLSQKAVL
160 170 180 190 200
KSYNAHSSEV NCVAACPGKD TIFLSCGEDG RILLWDTRKP KPATRIDFCA
210 220 230 240 250
SDTIPTSVTW HPEKDDTFAC GDETGNVSLV NIKNPDSAQT SAVHSQNITG
260 270 280 290 300
LAYSYHSSPF LASISEDCTV AVLDADFSEV FRDLSHRDFV TGVAWSPLDH
310 320 330
SKFTTVGWDH KVLHHHLPSE GRTENLIATK AED
Length:333
Mass (Da):36,052
Last modified:July 5, 2004 - v1
Checksum:i74673EB8C5765293
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068665 mRNA. Translation: AAH68665.1.
RefSeqiNP_001084703.1. NM_001091234.1.
UniGeneiXl.8563.

Genome annotation databases

GeneIDi414664.
KEGGixla:414664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068665 mRNA. Translation: AAH68665.1.
RefSeqiNP_001084703.1. NM_001091234.1.
UniGeneiXl.8563.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4G56X-ray2.95B/D2-333[»]
ProteinModelPortaliQ6NUD0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi101086. 2 interactions.

Proteomic databases

PRIDEiQ6NUD0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi414664.
KEGGixla:414664.

Organism-specific databases

CTDi79084.
XenbaseiXB-GENE-972901. wdr77.

Phylogenomic databases

HOVERGENiHBG052458.
KOiK13221.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMEP50_XENLA
AccessioniPrimary (citable) accession number: Q6NUD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.