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Protein

Methylosome protein 50

Gene

wdr77

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic component of the 20S prmt5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. Required for normal prmt5 methyltransferase activity.2 Publications

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome protein 50
Short name:
MEP-50
Alternative name(s):
WD repeat-containing protein 77
Gene namesi
Name:wdr77
Synonyms:mep50
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-972901. wdr77.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • histone methyltransferase complex Source: UniProtKB
  • methylosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004229731 – 333Methylosome protein 50Add BLAST333

Expressioni

Tissue specificityi

Detected in egg (at protein level).1 Publication

Interactioni

Subunit structurei

Heterotetramer; dimer of heterodimer with prmt5. Interacts with histone h2a and h4 and with nucleoplasmin.2 Publications

Protein-protein interaction databases

BioGridi101086. 2 interactors.

Structurei

Secondary structure

1333
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 26Combined sources8
Beta strandi28 – 30Combined sources3
Beta strandi32 – 37Combined sources6
Beta strandi39 – 43Combined sources5
Beta strandi46 – 53Combined sources8
Helixi60 – 62Combined sources3
Beta strandi64 – 68Combined sources5
Beta strandi73 – 79Combined sources7
Turni80 – 82Combined sources3
Beta strandi83 – 88Combined sources6
Beta strandi93 – 95Combined sources3
Beta strandi97 – 99Combined sources3
Turni100 – 103Combined sources4
Beta strandi109 – 111Combined sources3
Beta strandi118 – 123Combined sources6
Beta strandi125 – 134Combined sources10
Beta strandi139 – 143Combined sources5
Turni144 – 147Combined sources4
Beta strandi148 – 153Combined sources6
Beta strandi160 – 165Combined sources6
Beta strandi173 – 177Combined sources5
Beta strandi182 – 184Combined sources3
Beta strandi189 – 191Combined sources3
Beta strandi205 – 210Combined sources6
Beta strandi217 – 225Combined sources9
Beta strandi227 – 233Combined sources7
Helixi235 – 237Combined sources3
Beta strandi239 – 241Combined sources3
Beta strandi248 – 253Combined sources6
Beta strandi255 – 258Combined sources4
Beta strandi261 – 265Combined sources5
Beta strandi270 – 273Combined sources4
Beta strandi279 – 283Combined sources5
Beta strandi290 – 295Combined sources6
Beta strandi297 – 299Combined sources3
Beta strandi302 – 307Combined sources6
Beta strandi312 – 316Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G56X-ray2.95B/D2-333[»]
ProteinModelPortaliQ6NUD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati16 – 59WD 1Add BLAST44
Repeati68 – 106WD 2Add BLAST39
Repeati113 – 152WD 3Add BLAST40
Repeati155 – 195WD 4Add BLAST41
Repeati199 – 240WD 5Add BLAST42
Repeati243 – 283WD 6Add BLAST41
Repeati285 – 328WD 7Add BLAST44

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOVERGENiHBG052458.
KOiK13221.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NUD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKGSAWGRP VTAPACMEVQ IGAVRYRRDG ALLLAASSLS SRTWGGSIWV
60 70 80 90 100
FKDPEGAPNE SLCTAGVQTE AGVTDVAWVS EKGILVASDS GAVELWEILE
110 120 130 140 150
KESLLVNKFA KYEHDDIVKT LSVFSDGTQA VSGGKDFSVK VWDLSQKAVL
160 170 180 190 200
KSYNAHSSEV NCVAACPGKD TIFLSCGEDG RILLWDTRKP KPATRIDFCA
210 220 230 240 250
SDTIPTSVTW HPEKDDTFAC GDETGNVSLV NIKNPDSAQT SAVHSQNITG
260 270 280 290 300
LAYSYHSSPF LASISEDCTV AVLDADFSEV FRDLSHRDFV TGVAWSPLDH
310 320 330
SKFTTVGWDH KVLHHHLPSE GRTENLIATK AED
Length:333
Mass (Da):36,052
Last modified:July 5, 2004 - v1
Checksum:i74673EB8C5765293
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068665 mRNA. Translation: AAH68665.1.
RefSeqiNP_001084703.1. NM_001091234.1.
UniGeneiXl.8563.

Genome annotation databases

GeneIDi414664.
KEGGixla:414664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068665 mRNA. Translation: AAH68665.1.
RefSeqiNP_001084703.1. NM_001091234.1.
UniGeneiXl.8563.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G56X-ray2.95B/D2-333[»]
ProteinModelPortaliQ6NUD0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi101086. 2 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi414664.
KEGGixla:414664.

Organism-specific databases

CTDi79084.
XenbaseiXB-GENE-972901. wdr77.

Phylogenomic databases

HOVERGENiHBG052458.
KOiK13221.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMEP50_XENLA
AccessioniPrimary (citable) accession number: Q6NUD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: July 5, 2004
Last modified: October 5, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.