Ubiquitin thioesterase zranb1-B - Xenopus laevis (African clawed frog)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase zranb1-B
EC=3.4.19.12

Alternative name(s):
Zinc finger Ran-binding domain-containing protein 1B

Gene names

Name:

zranb1-b

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. May play a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The OTU domain mediates the deubiquitinating activity By similarity. The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin By similarity.
Sequence similarities	Belongs to the peptidase C64 family. Contains 2 ANK repeats. Contains 1 OTU domain. Contains 3 RanBP2-type zinc fingers.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Wnt signaling pathway
Cellular component	Cytoplasm Nucleus
Domain	ANK repeat Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell migration Inferred from sequence or structural similarity. Source: UniProtKB cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 701

701

Ubiquitin thioesterase zranb1-B

PRO_0000361557

Regions

Repeat

253 – 283

31

ANK 1

Repeat

306 – 333

28

ANK 2

Domain

425 – 585

161

OTU

Zinc finger

3 – 33

31

RanBP2-type 1

Zinc finger

79 – 108

30

RanBP2-type 2

Zinc finger

143 – 173

31

RanBP2-type 3

Sites

Active site

436

1

Nucleophile By similarity

Active site

578

1

Proton acceptor By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6NUB7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: FDCD31885AC1A180
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FASTA

701

79,950

        10         20         30         40         50         60 
MTEDGIKWAC EYCTFENWPS AIKCTMCRAP RPSGAIITEE PFKNRTPDVG SMEREIGSPL 

        70         80         90        100        110        120 
ICPDSSARPR VKSSYSMETS SKWSCQICTY LNWPRAIRCT QCLSQRRTRS PTESPQSSGS 

       130        140        150        160        170        180 
GLRSIPGPID PCEEYNDRNK LNIKGQHWTC SACTYENCAK AKKCVVCDHP TPNNMDAIEL 

       190        200        210        220        230        240 
ANTDEASSII NEQDRARWRG GCSSSNSQRR SPPTSKRDSD MDFQRIELAG AVGSKEEFEL 

       250        260        270        280        290        300 
DLKKLKQIKN RMRKTDWLFL NACVGVVEGD LSAVEAYKTS GGDIARQLSA DEVRLLNRPS 

       310        320        330        340        350        360 
AFDVGYTLVH LSIRFQRQDM LAILLTEVAQ HAAKCIPAMV CPELTEQIRR EIAASVHQRK 

       370        380        390        400        410        420 
GDFACYFLTD LVTFTLPADI EDLPPTVQEK LFDEVLDRDV QKELEEESPI INWSLELGTR 

       430        440        450        460        470        480 
LDSRLYALWN RTAGDCLLDS VLQATWGIYD KDSVLRKALH DSLRDCSHWF YSRWKEWESW 

       490        500        510        520        530        540 
YSQSFGLHFS LREEQWQEDW AFILSLASQP GASLEQTHIF VLAHILRRPI IVYGVKYYKS 

       550        560        570        580        590        600 
FRGETLGYTR FQGVYLPLLW EQSFCWKSPI ALGYTRGHFS ALVAMENDGF DNRGAGANLN 

       610        620        630        640        650        660 
TDDDVTVTFL PLVDSERKLL HVHFLSAQEL GNEDQQEKLL REWMDCCVTE GGVLVAMQKS 

       670        680        690        700 
SRRRNHPLVT QMVEKWLDGY RQIRPCTALS DGEEDEDDED E

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References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC068679 mRNA. Translation: AAH68679.1.
RefSeq	NP_001084698.1. NM_001091229.1.
UniGene	Xl.9866.
3D structure databases
HSSP	HSSP built from PDB template 1Q5W based on UniProtKB Q9ES54.
ModBase	Search...
Protein family/group databases
MEROPS	C64.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	414659.
KEGG	xla:414659.
Organism-specific databases
CTD	414659.
Xenbase	XB-GENE-6252281. zranb1.
Phylogenomic databases
HOVERGEN	HBG058978.
KO	K11862.
Family and domain databases
InterPro	IPR003323. OTU. IPR001876. Znf_RanBP2. [Graphical view]
Pfam	PF02338. OTU. 1 hit. PF00641. zf-RanBP. 2 hits. [Graphical view]
SMART	SM00547. ZnF_RBZ. 3 hits. [Graphical view]
PROSITE	PS50088. ANK_REPEAT. False negative. PS50802. OTU. 1 hit. PS01358. ZF_RANBP2_1. 3 hits. PS50199. ZF_RANBP2_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ZRN1B_XENLA

Accession

Primary (citable) accession number: Q6NUB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents