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Q6NUB7 (ZRN1B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin thioesterase zranb1-B

EC=3.4.19.12
Alternative name(s):
Zinc finger Ran-binding domain-containing protein 1B
Gene names
Name:zranb1-b
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length701 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. May play a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Domain

The OTU domain mediates the deubiquitinating activity By similarity.

The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin By similarity.

Sequence similarities

Belongs to the peptidase C64 family.

Contains 2 ANK repeats.

Contains 1 OTU domain.

Contains 3 RanBP2-type zinc fingers.

Ontologies

Keywords
   Biological processUbl conjugation pathway
Wnt signaling pathway
   Cellular componentCytoplasm
Nucleus
   DomainANK repeat
Repeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Protease
Thiol protease
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

cytoskeleton organization

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of Wnt signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

protein K29-linked deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K33-linked deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein K63-linked deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of cell morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionubiquitin thiolesterase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-specific protease activity

Inferred from sequence or structural similarity. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 701701Ubiquitin thioesterase zranb1-B
PRO_0000361557

Regions

Repeat253 – 28331ANK 1
Repeat306 – 33328ANK 2
Domain425 – 585161OTU
Zinc finger3 – 3331RanBP2-type 1
Zinc finger79 – 10830RanBP2-type 2
Zinc finger143 – 17331RanBP2-type 3

Sites

Active site4361Nucleophile By similarity
Active site5781Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6NUB7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: FDCD31885AC1A180

FASTA70179,950
        10         20         30         40         50         60 
MTEDGIKWAC EYCTFENWPS AIKCTMCRAP RPSGAIITEE PFKNRTPDVG SMEREIGSPL 

        70         80         90        100        110        120 
ICPDSSARPR VKSSYSMETS SKWSCQICTY LNWPRAIRCT QCLSQRRTRS PTESPQSSGS 

       130        140        150        160        170        180 
GLRSIPGPID PCEEYNDRNK LNIKGQHWTC SACTYENCAK AKKCVVCDHP TPNNMDAIEL 

       190        200        210        220        230        240 
ANTDEASSII NEQDRARWRG GCSSSNSQRR SPPTSKRDSD MDFQRIELAG AVGSKEEFEL 

       250        260        270        280        290        300 
DLKKLKQIKN RMRKTDWLFL NACVGVVEGD LSAVEAYKTS GGDIARQLSA DEVRLLNRPS 

       310        320        330        340        350        360 
AFDVGYTLVH LSIRFQRQDM LAILLTEVAQ HAAKCIPAMV CPELTEQIRR EIAASVHQRK 

       370        380        390        400        410        420 
GDFACYFLTD LVTFTLPADI EDLPPTVQEK LFDEVLDRDV QKELEEESPI INWSLELGTR 

       430        440        450        460        470        480 
LDSRLYALWN RTAGDCLLDS VLQATWGIYD KDSVLRKALH DSLRDCSHWF YSRWKEWESW 

       490        500        510        520        530        540 
YSQSFGLHFS LREEQWQEDW AFILSLASQP GASLEQTHIF VLAHILRRPI IVYGVKYYKS 

       550        560        570        580        590        600 
FRGETLGYTR FQGVYLPLLW EQSFCWKSPI ALGYTRGHFS ALVAMENDGF DNRGAGANLN 

       610        620        630        640        650        660 
TDDDVTVTFL PLVDSERKLL HVHFLSAQEL GNEDQQEKLL REWMDCCVTE GGVLVAMQKS 

       670        680        690        700 
SRRRNHPLVT QMVEKWLDGY RQIRPCTALS DGEEDEDDED E 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC068679 mRNA. Translation: AAH68679.1.
RefSeqNP_001084698.1. NM_001091229.1.
UniGeneXl.9866.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC64.004.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID414659.
KEGGxla:414659.

Organism-specific databases

CTD414659.
XenbaseXB-GENE-6252281. zranb1.

Phylogenomic databases

HOVERGENHBG058978.
KOK11862.

Family and domain databases

InterProIPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamPF02338. OTU. 1 hit.
PF00641. zf-RanBP. 2 hits.
[Graphical view]
SMARTSM00547. ZnF_RBZ. 3 hits.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameZRN1B_XENLA
AccessionPrimary (citable) accession number: Q6NUB7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries