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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

mettl1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei162 – 1621S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei165 – 1651UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
  2. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA (guanine-N7)-methylation Source: GOC
  2. tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Methyltransferase-like protein 1UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:mettl1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252283. mettl1.

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273tRNA (guanine-N(7)-)-methyltransferasePRO_0000370559Add
BLAST

Proteomic databases

PRIDEiQ6NU94.

Interactioni

Subunit structurei

Forms a complex with wdr4.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6NU94.
SMRiQ6NU94. Positions 39-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni109 – 1102S-adenosyl-L-methionine bindingUniRule annotation
Regioni142 – 1432S-adenosyl-L-methionine bindingUniRule annotation
Regioni240 – 2423S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

KOiK03439.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NU94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADTQAMASD RGVAVTLPQK RYYRQRAHSN PMADHTFQYP VKPEVMDWSE
60 70 80 90 100
YYPEFFKPLV PDCAHDDAKD LQERKEQHQV EFADVGCGYG GLLVALSPLF
110 120 130 140 150
PNTLMLGLEI RVKVSDYVQD RIKSLRASHL GQYQNIACIR SNAMKYLPNF
160 170 180 190 200
FKKGQLSKMF FLFPDPHFKK TKHKWRIISP TLLAEYSYAL RVGGMVYTIT
210 220 230 240 250
DVEEVHEWMV KHFTEHPLFE RVEKEELVSD IIVDKLGTST EEGKKVQRNK
260 270
GQNFLAVFRR IENRTFIQRD SQQ
Length:273
Mass (Da):31,843
Last modified:July 4, 2004 - v1
Checksum:i9E0BE0D49811ADAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068703 mRNA. Translation: AAH68703.1.
RefSeqiNP_001084693.1. NM_001091224.1.
UniGeneiXl.43809.

Genome annotation databases

GeneIDi414654.
KEGGixla:414654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC068703 mRNA. Translation: AAH68703.1.
RefSeqiNP_001084693.1. NM_001091224.1.
UniGeneiXl.43809.

3D structure databases

ProteinModelPortaliQ6NU94.
SMRiQ6NU94. Positions 39-263.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6NU94.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi414654.
KEGGixla:414654.

Organism-specific databases

CTDi4234.
XenbaseiXB-GENE-6252283. mettl1.

Phylogenomic databases

KOiK03439.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiTRMB_XENLA
AccessioniPrimary (citable) accession number: Q6NU94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2009
Last sequence update: July 4, 2004
Last modified: January 6, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.