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Q6NU94 (TRMB_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Methyltransferase-like protein 1
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:mettl1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with wdr4 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370559

Regions

Region109 – 1102S-adenosyl-L-methionine binding By similarity
Region142 – 1432S-adenosyl-L-methionine binding By similarity
Region240 – 2423S-adenosyl-L-methionine binding By similarity

Sites

Active site1651 By similarity
Binding site861S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1621S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6NU94 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 9E0BE0D49811ADAC

FASTA27331,843
        10         20         30         40         50         60 
MADTQAMASD RGVAVTLPQK RYYRQRAHSN PMADHTFQYP VKPEVMDWSE YYPEFFKPLV 

        70         80         90        100        110        120 
PDCAHDDAKD LQERKEQHQV EFADVGCGYG GLLVALSPLF PNTLMLGLEI RVKVSDYVQD 

       130        140        150        160        170        180 
RIKSLRASHL GQYQNIACIR SNAMKYLPNF FKKGQLSKMF FLFPDPHFKK TKHKWRIISP 

       190        200        210        220        230        240 
TLLAEYSYAL RVGGMVYTIT DVEEVHEWMV KHFTEHPLFE RVEKEELVSD IIVDKLGTST 

       250        260        270 
EEGKKVQRNK GQNFLAVFRR IENRTFIQRD SQQ 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC068703 mRNA. Translation: AAH68703.1.
RefSeqNP_001084693.1. NM_001091224.1.
UniGeneXl.43809.

3D structure databases

ProteinModelPortalQ6NU94.
SMRQ6NU94. Positions 39-263.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ6NU94.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID414654.
KEGGxla:414654.

Organism-specific databases

CTD4234.
XenbaseXB-GENE-6252283. mettl1.

Phylogenomic databases

KOK03439.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_XENLA
AccessionPrimary (citable) accession number: Q6NU94
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways