Q6NU94 (TRMB_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Methyltransferase-like protein 1
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:

mettl1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with wdr4 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370559
Regions
	Region	109 – 110	2	S-adenosyl-L-methionine binding By similarity
	Region	142 – 143	2	S-adenosyl-L-methionine binding By similarity
	Region	240 – 242	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	165	1	By similarity
	Binding site	86	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	162	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NU94 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9E0BE0D49811ADAC	FASTA	273	31,843
	10 20 30 40 50 60 MADTQAMASD RGVAVTLPQK RYYRQRAHSN PMADHTFQYP VKPEVMDWSE YYPEFFKPLV 70 80 90 100 110 120 PDCAHDDAKD LQERKEQHQV EFADVGCGYG GLLVALSPLF PNTLMLGLEI RVKVSDYVQD 130 140 150 160 170 180 RIKSLRASHL GQYQNIACIR SNAMKYLPNF FKKGQLSKMF FLFPDPHFKK TKHKWRIISP 190 200 210 220 230 240 TLLAEYSYAL RVGGMVYTIT DVEEVHEWMV KHFTEHPLFE RVEKEELVSD IIVDKLGTST 250 260 270 EEGKKVQRNK GQNFLAVFRR IENRTFIQRD SQQ

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC068703 mRNA. Translation: AAH68703.1.
RefSeq	NP_001084693.1. NM_001091224.1.
UniGene	Xl.43809.
3D structure databases
ProteinModelPortal	Q6NU94.
SMR	Q6NU94. Positions 39-263.
ModBase	Search...
Proteomic databases
PRIDE	Q6NU94.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	414654.
KEGG	xla:414654.
Organism-specific databases
CTD	4234.
Xenbase	XB-GENE-6252283. mettl1.
Phylogenomic databases
KO	K03439.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_XENLA

Accession

Primary (citable) accession number: Q6NU94

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	July 5, 2004
Last modified:	November 28, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program