Reviewed, UniProtKB/Swiss-Prot Q6NU46 (THILA_XENLA)

Names and origin

Protein names

Recommended name:
    Acetyl-CoA acetyltransferase A, mitochondrial
    EC=2.3.1.9
Alternative name(s):
    Acetoacetyl-CoA thiolase A

Gene names

Name:

acat1-A

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a major role in ketone body metabolism By similarity.
Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Potassium
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Mitochondrion By similarity
	Chain	34 – 420	387	Acetyl-CoA acetyltransferase A, mitochondrial		PRO_0000356276
Regions
	Region	251 – 253	3	Coenzyme A binding By similarity
Sites
	Active site	119	1	Acyl-thioester intermediate By similarity
	Active site	378	1	Proton acceptor By similarity
	Active site	406	1	Proton acceptor By similarity
	Metal binding	212	1	Potassium By similarity
	Metal binding	273	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	274	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	374	1	Potassium; via carbonyl oxygen By similarity
	Binding site	212	1	Coenzyme A By similarity
	Binding site	256	1	Coenzyme A By similarity
	Binding site	277	1	Coenzyme A By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NU46-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 19527789ABD7FFF5	FASTA	420	44,128
	10 20 30 40 50 60 MAFCGTRTAA RLSHSTRALH NTHRNFASQR TLNEVVIASA ARTPIGSFQG TLSSLPATKL 70 80 90 100 110 120 GSIAIKAAVE RAGIPADEVK EVYMGNVLQA GQGQAPSRQA TLGAGLAIST PTTTINKVCA 130 140 150 160 170 180 SGMKSVMLAA QSLMCGHQQV MVAGGMESMS NVPYCMSRGA TPYGGVKLED LIVKDGLTDV 190 200 210 220 230 240 YNKIHMGNCA ENTAKKFSIS REEQDSFAIH SYTRSKAAWD SGLIANEIAP VTIAQKGKPD 250 260 270 280 290 300 IIVQEDEEYK RVDFSKFPKL KTVFQKDNGT VTAANSSTLN DGAAALLLMT TEAANRLNVT 310 320 330 340 350 360 PLARIVAFAD AAVDPIDFPI APAYAVPKLL SEAGLKKEDI AMWEINEAFS VVVLANIKML 370 380 390 400 410 420 GIDPARVNMN GGAVSLGHPI GMSGARIVGH MAHALKKGQF GIAGICNGGG GASAVLIEKL

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC068755 mRNA. Translation: AAH68755.1.
RefSeq	NP_001084679.1.
UniGene	Xl.25595
3D structure databases
HSSP	HSSP built from PDB template 1AFW based on UniProtKB P27796.
SMR	Q6NU46. Positions 28-420.
ModBase	Search...
Proteomic databases
PRIDE	Q6NU46.
Genome annotation databases
GeneID	414639.
KEGG	xla:414639.
Organism-specific databases
CTD	414639.
Xenbase	XB-FEAT-977281. acat1.
Phylogenomic databases
HOVERGEN	Q6NU46.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THILA_XENLA

Accession

Primary (citable) accession number: Q6NU46

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	July 5, 2004
Last modified:	September 1, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project