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Q6NTR6

- UP44A_XENLA

UniProt

Q6NTR6 - UP44A_XENLA

Protein

Ubiquitin carboxyl-terminal hydrolase 44-A

Gene

usp44-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Deubiquitinase that plays a key role in the spindle checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of cdc20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C) By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei283 – 2831NucleophilePROSITE-ProRule annotation
    Active sitei625 – 6251Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri24 – 8562UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB
    2. ubiquitin thiolesterase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. anaphase Source: UniProtKB
    2. mitotic nuclear division Source: UniProtKB-KW
    3. negative regulation of mitotic anaphase-promoting complex activity Source: UniProtKB
    4. protein deubiquitination Source: UniProtKB
    5. regulation of spindle checkpoint Source: UniProtKB
    6. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 44-A (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 44-A
    Ubiquitin thioesterase 44-A
    Ubiquitin-specific-processing protease 44-A
    Gene namesi
    Name:usp44-a
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-1014118. usp44.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 690690Ubiquitin carboxyl-terminal hydrolase 44-APRO_0000395813Add
    BLAST

    Proteomic databases

    PRIDEiQ6NTR6.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6NTR6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini274 – 667394USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP44 subfamily.Curated
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri24 – 8562UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG018027.
    KOiK11834.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00290. ZnF_UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6NTR6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKCKHVGRL RLAQDHSILN PQKWHCVDCN TTESVWACLS CSHVACGRYI    50
    EEHALRHFQD SKHPLALEVN ELYVFCYLCD DYVLNDNTTG DLKLLRSTLS 100
    AIKSQNYDCT TRSGRTLRSM VTADDSFISH EGAQAFLQNE DRAFTALWHR 150
    RHALLGKVFR SWFALTPKGK QRLEEERLRE EAEQKREEAR KKRQQLKRKL 200
    KEEMESTSPR KSSRLQHQIQ PSPKTELPSV QKMNQKNSPT TKQKTPAPTS 250
    DKACFKKIGN SPIKRKPTVT PGVTGLRNLG NTCYMNSILQ ILSHLHVFRE 300
    CFLQLDLNQT QELLAAAGSG KTRLSSKYPP GAELPRVTQK HTKVQRSLAR 350
    RQSFSLGLSG GASNSRNMEL IQPKEPSSKH ISLCHELHTL FQVMWSGKWA 400
    LVSPFAMLHS VWRLIPAFRG YAQQDAQEFL CELLDKVQQE LETTGTRYPA 450
    LIPTSQRKLI KQVLNVVNNI FHGQLLSQVT CLVCDHKSNT IEPFWDLSLE 500
    FPERYHFNGK ETASQRPCLL TEMLAKFTET EALEGKIYAC DQCNKAQKQL 550
    MVCRLPQVLR LHLKRFRWSG RNHREKIGVH VRFDQMLNME PYCCRESTAA 600
    LRADCFIYDL SSVVMHHGKG FGSGHYTAFC YNPEGGFWVH CNDSKLHSCT 650
    VEEVCKAQAY ILFYTQRVTQ ENGHLSDTLP VHGSPQSPPR 690
    Length:690
    Mass (Da):79,011
    Last modified:July 5, 2004 - v1
    Checksum:i77A3C98DB6F8E5BA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC068889 mRNA. Translation: AAH68889.1.
    RefSeqiNP_001084641.1. NM_001091172.1.
    UniGeneiXl.6668.

    Genome annotation databases

    GeneIDi414600.
    KEGGixla:414600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC068889 mRNA. Translation: AAH68889.1 .
    RefSeqi NP_001084641.1. NM_001091172.1.
    UniGenei Xl.6668.

    3D structure databases

    ProteinModelPortali Q6NTR6.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q6NTR6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 414600.
    KEGGi xla:414600.

    Organism-specific databases

    CTDi 414600.
    Xenbasei XB-GENE-1014118. usp44.

    Phylogenomic databases

    HOVERGENi HBG018027.
    KOi K11834.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00290. ZnF_UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiUP44A_XENLA
    AccessioniPrimary (citable) accession number: Q6NTR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3