ID   CGB2_HUMAN              Reviewed;         163 AA.
AC   Q6NT52; B9ZVM5; H9KV56;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   28-MAR-2018, sequence version 5.
DT   27-MAR-2024, entry version 137.
DE   RecName: Full=Choriogonadotropin subunit beta variant 2;
DE   Flags: Precursor;
GN   Name=CGB2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=18048458; DOI=10.1093/molehr/gam082;
RA   Rull K., Hallast P., Uuskula L., Jackson J., Punab M., Salumets A.,
RA   Campbell R.K., Laan M.;
RT   "Fine-scale quantification of HCG beta gene transcription in human
RT   trophoblastic and non-malignant non-trophoblastic tissues.";
RL   Mol. Hum. Reprod. 14:23-31(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA   Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA   Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA   Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA   Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA   Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA   Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA   Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA   Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA   McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA   Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA   Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA   She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA   Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA   Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   MISCELLANEOUS.
RX   PubMed=11861891; DOI=10.1093/oxfordjournals.molbev.a004085;
RA   Maston G.A., Ruvolo M.;
RT   "Chorionic gonadotropin has a recent origin within primates and an
RT   evolutionary history of selection.";
RL   Mol. Biol. Evol. 19:320-335(2002).
RN   [5]
RP   TISSUE SPECIFICITY.
RX   PubMed=16123088; DOI=10.1093/humrep/dei261;
RA   Rull K., Laan M.;
RT   "Expression of beta-subunit of HCG genes during normal and failed
RT   pregnancy.";
RL   Hum. Reprod. 20:3360-3368(2005).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed in placenta, testis and pituitary.
CC       {ECO:0000269|PubMed:16123088, ECO:0000269|PubMed:18048458}.
CC   -!- MISCELLANEOUS: Encoded by a cluster of genes that have evolved by
CC       duplication from LHB. HCG-beta is encoded by six non-allelic genes
CC       (CGB) clustered on chromosome 19q13.3 and named CGB1, CGB2, CGB3, CGB5,
CC       CGB7 and CGB8. Two specific hCGb proteins that differ by three amino
CC       acids in positions 2,4 and 117 have been described: type 1 (CGB7) and
CC       type 2 (CGB3, CGB5, CGB8). The CGB gene first arose in the common
CC       ancestor of the anthropoid primates. {ECO:0000269|PubMed:11861891}.
CC   -!- SIMILARITY: Belongs to the glycoprotein hormones subunit beta family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH69367.2; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
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DR   EMBL; AC008687; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC069367; AAH69367.2; ALT_SEQ; mRNA.
DR   CCDS; CCDS12750.2; -.
DR   RefSeq; NP_203696.2; NM_033378.1.
DR   AlphaFoldDB; Q6NT52; -.
DR   SMR; Q6NT52; -.
DR   IntAct; Q6NT52; 15.
DR   MINT; Q6NT52; -.
DR   STRING; 9606.ENSP00000352295; -.
DR   GlyCosmos; Q6NT52; 2 sites, No reported glycans.
DR   GlyGen; Q6NT52; 2 sites.
DR   PhosphoSitePlus; Q6NT52; -.
DR   BioMuta; CGB2; -.
DR   DMDM; 281185508; -.
DR   MassIVE; Q6NT52; -.
DR   MaxQB; Q6NT52; -.
DR   PaxDb; 9606-ENSP00000352295; -.
DR   PeptideAtlas; Q6NT52; -.
DR   ProteomicsDB; 46213; -.
DR   Antibodypedia; 31875; 89 antibodies from 12 providers.
DR   DNASU; 114336; -.
DR   Ensembl; ENST00000359342.7; ENSP00000352295.6; ENSG00000104818.15.
DR   GeneID; 114336; -.
DR   KEGG; hsa:114336; -.
DR   MANE-Select; ENST00000359342.7; ENSP00000352295.6; NM_033378.2; NP_203696.2.
DR   AGR; HGNC:16722; -.
DR   CTD; 114336; -.
DR   DisGeNET; 114336; -.
DR   GeneCards; CGB2; -.
DR   HGNC; HGNC:16722; CGB2.
DR   HPA; ENSG00000104818; Tissue enhanced (testis).
DR   MIM; 608824; gene.
DR   neXtProt; NX_Q6NT52; -.
DR   OpenTargets; ENSG00000104818; -.
DR   VEuPathDB; HostDB:ENSG00000104818; -.
DR   eggNOG; ENOG502S49V; Eukaryota.
DR   GeneTree; ENSGT00940000163162; -.
DR   InParanoid; Q6NT52; -.
DR   OMA; EACPFCI; -.
DR   OrthoDB; 2945053at2759; -.
DR   PhylomeDB; Q6NT52; -.
DR   TreeFam; TF332940; -.
DR   PathwayCommons; Q6NT52; -.
DR   SignaLink; Q6NT52; -.
DR   BioGRID-ORCS; 114336; 27 hits in 656 CRISPR screens.
DR   GenomeRNAi; 114336; -.
DR   Pharos; Q6NT52; Tdark.
DR   PRO; PR:Q6NT52; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q6NT52; Protein.
DR   Bgee; ENSG00000104818; Expressed in primordial germ cell in gonad and 31 other cell types or tissues.
DR   ExpressionAtlas; Q6NT52; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005179; F:hormone activity; IMP:AgBase.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   CDD; cd00069; GHB_like; 1.
DR   Gene3D; 2.10.90.10; Cystine-knot cytokines; 1.
DR   InterPro; IPR029034; Cystine-knot_cytokine.
DR   InterPro; IPR006208; Glyco_hormone_CN.
DR   InterPro; IPR001545; Gonadotropin_bsu.
DR   InterPro; IPR018245; Gonadotropin_bsu_CS.
DR   PANTHER; PTHR11515; GLYCOPROTEIN HORMONE BETA CHAIN; 1.
DR   PANTHER; PTHR11515:SF11; LUTROPIN SUBUNIT BETA; 1.
DR   Pfam; PF00007; Cys_knot; 1.
DR   SMART; SM00068; GHB; 1.
DR   SUPFAM; SSF57501; Cystine-knot cytokines; 1.
DR   PROSITE; PS00261; GLYCO_HORMONE_BETA_1; 1.
DR   PROSITE; PS00689; GLYCO_HORMONE_BETA_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hormone; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..163
FT                   /note="Choriogonadotropin subunit beta variant 2"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000342549"
FT   REGION          129..163
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        142..163
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        31
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        48
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        27..75
FT                   /evidence="ECO:0000250"
FT   DISULFID        41..90
FT                   /evidence="ECO:0000250"
FT   DISULFID        44..128
FT                   /evidence="ECO:0000250"
FT   DISULFID        52..106
FT                   /evidence="ECO:0000250"
FT   DISULFID        56..108
FT                   /evidence="ECO:0000250"
FT   DISULFID        111..118
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   163 AA;  17374 MW;  823CEF334B9183F6 CRC64;
     MSKGLLLLLL LSMGGTWASK EPLRPRCRPI NATLAVEKEG CPVCITVNTT ICAGYCPTMT
     RVLQGVLPAL PQVVCNYRDV RFESIRLPGC PRGVNPVVSY AVALSCQCAL CRRSTTDCGG
     PKDHPLTCDD PRFQASSSSK APPPSLPSPS RLPGPSDTPI LPQ
//