Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6NSQ9 (G6PC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphatase 3

Short name=G-6-Pase 3
Short name=G6Pase 3
EC=3.1.3.9
Alternative name(s):
Ubiquitous glucose-6-phosphatase catalytic subunit-related protein
Gene names
Name:G6pc3
Synonyms:Ugrp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. May form with the glucose-6-phosphate transporter (SLC37A4/G6PT) a ubiquitously expressed complex responsible for glucose production through glycogenolysis and gluconeogenesis. Probably required for normal neutrophil function. Ref.5 Ref.7 Ref.8 Ref.9

Catalytic activity

D-glucose 6-phosphate + H2O = D-glucose + phosphate.

Enzyme regulation

Inhibited by vanadate By similarity.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Widely expressed. Highly expressed in heart, brain, kidney and testis and to a lower extent in lung, spleen, stomach, small intestine, skeletal muscle and uterus. Expressed in muscle, brain, thymus, lung, kidney, spleen and pancreas (at protein level). Ref.5 Ref.6 Ref.7

Disruption phenotype

Mice display reduced glucose-6-phosphate hydrolytic activity in the brain. No phenotypic difference was noted at birth but 4 months old female mice display growth retardation. Mutant mice exhibit a decreased plasma cholesterol concentration and an increased plasma glucagon concentration but no difference in blood glucose concentration (Ref.8). Mice display neutropenia and neutrophil dysfunctions. Ref.8 Ref.9

Sequence similarities

Belongs to the glucose-6-phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Glucose-6-phosphatase 3
PRO_0000334513

Regions

Topological domain1 – 2424Lumenal Potential
Transmembrane25 – 4521Helical; Potential
Topological domain46 – 5611Cytoplasmic Potential
Transmembrane57 – 7721Helical; Potential
Topological domain78 – 10831Lumenal Potential
Transmembrane109 – 12921Helical; Potential
Topological domain130 – 1389Cytoplasmic Potential
Transmembrane139 – 15921Helical; Potential
Topological domain160 – 1678Lumenal Potential
Transmembrane168 – 18619Helical; Potential
Topological domain187 – 19711Cytoplasmic Potential
Transmembrane198 – 21821Helical; Potential
Topological domain219 – 25436Lumenal Potential
Transmembrane255 – 27319Helical; Potential
Topological domain274 – 28310Cytoplasmic Potential
Transmembrane284 – 30421Helical; Potential
Topological domain305 – 3073Lumenal Potential
Transmembrane308 – 32821Helical; Potential
Topological domain329 – 34618Cytoplasmic Potential

Sites

Active site1141Proton donor Potential
Active site1671Nucleophile By similarity
Binding site791Substrate Potential
Binding site1611Substrate Potential

Experimental info

Sequence conflict1361R → Q in BAE38159. Ref.2
Sequence conflict2071M → L in AAO39164. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6NSQ9 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 50CDA634BCABA014

FASTA34638,782
        10         20         30         40         50         60 
MESTLSAGII MAEALQNRLP GLENMWLWVT FLGDPKNLFQ FCFPAAYYAS RRLGISVLWI 

        70         80         90        100        110        120 
TFIAEWLNLV FKWFLFGDRP FWWVHESGYS TQTPIQIHQF PSSCETGPGS PSGHCMITGA 

       130        140        150        160        170        180 
ALWPVMTAIS SQVASRSRSP WVRVIPGLAY CTFLLAVGLS RVFLLAHFPH QVLGGLIVGA 

       190        200        210        220        230        240 
ALGWLMSPRV PMERELSFYG LTALALMLGA SLMYWTLFTL GLDLSWSINL ASKWCERPEW 

       250        260        270        280        290        300 
VHMDSRPFAS LSRDSGSALG LGIALHTPCY AQIRRAHLGN GQKIACFVLA MGLLVFLEWL 

       310        320        330        340 
GYPPQISLFY IFNFLKYTLW PCLVLALVPW VVHTLSDQEA PPIRSS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a glucose-6-phosphatase catalytic subunit-related sequence expressed in rodent tissues."
Middleditch C., Darakhshan F., Bonnefont J., Guionie O., Burchell A., Clottes E.
Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CD-1.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon.
[5]"A potential new role for muscle in blood glucose homeostasis."
Shieh J.-J., Pan C.-J., Mansfield B.C., Chou J.Y.
J. Biol. Chem. 279:26215-26219(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]"Identification and characterization of a cDNA and the gene encoding the mouse ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein."
Boustead J.N., Martin C.C., Oeser J.K., Svitek C.A., Hunter S.I., Hutton J.C., O'Brien R.M.
J. Mol. Endocrinol. 32:33-53(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[7]"Brain contains a functional glucose-6-phosphatase complex capable of endogenous glucose production."
Ghosh A., Cheung Y.Y., Mansfield B.C., Chou J.Y.
J. Biol. Chem. 280:11114-11119(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[8]"Deletion of the gene encoding the ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein (UGRP)/glucose-6-phosphatase catalytic subunit-beta results in lowered plasma cholesterol and elevated glucagon."
Wang Y., Oeser J.K., Yang C., Sarkar S., Hackl S.I., Hasty A.H., McGuinness O.P., Paradee W., Hutton J.C., Powell D.R., O'Brien R.M.
J. Biol. Chem. 281:39982-39989(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[9]"Impaired neutrophil activity and increased susceptibility to bacterial infection in mice lacking glucose-6-phosphatase-beta."
Cheung Y.Y., Kim S.Y., Yiu W.H., Pan C.-J., Jun H.-S., Ruef R.A., Lee E.J., Westphal H., Mansfield B.C., Chou J.Y.
J. Clin. Invest. 117:784-793(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY186239 mRNA. Translation: AAO39164.1.
AK165395 mRNA. Translation: BAE38159.1.
AL954730 Genomic DNA. Translation: CAM25070.1.
BC069959 mRNA. Translation: AAH69959.1.
CCDSCCDS25491.1.
RefSeqNP_787949.2. NM_175935.3.
UniGeneMm.22385.

3D structure databases

ProteinModelPortalQ6NSQ9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000077995.

PTM databases

PhosphoSiteQ6NSQ9.

Proteomic databases

PaxDbQ6NSQ9.
PRIDEQ6NSQ9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070334; ENSMUSP00000064276; ENSMUSG00000034793.
ENSMUST00000078975; ENSMUSP00000077995; ENSMUSG00000034793.
GeneID68401.
KEGGmmu:68401.
UCSCuc007lqt.2. mouse.

Organism-specific databases

CTD92579.
MGIMGI:1915651. G6pc3.

Phylogenomic databases

eggNOGNOG82628.
GeneTreeENSGT00510000046465.
HOGENOMHOG000264239.
HOVERGENHBG003560.
InParanoidQ6NSQ9.
KOK01084.
OMAKWFLFGD.
OrthoDBEOG73NG4N.
PhylomeDBQ6NSQ9.
TreeFamTF324388.

Enzyme and pathway databases

UniPathwayUPA00138.

Gene expression databases

BgeeQ6NSQ9.
GenevestigatorQ6NSQ9.

Family and domain databases

Gene3D1.20.144.10. 1 hit.
InterProIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMSSF48317. SSF48317. 1 hit.
ProtoNetSearch...

Other

NextBio327124.
PROQ6NSQ9.
SOURCESearch...

Entry information

Entry nameG6PC3_MOUSE
AccessionPrimary (citable) accession number: Q6NSQ9
Secondary accession number(s): Q3TND0, Q811R8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot