Reviewed, UniProtKB/Swiss-Prot Q6NSQ9 (G6PC3_MOUSE)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphatase 3
      Short name=G-6-Pase 3
      Short name=G6Pase 3
    EC=3.1.3.9
Alternative name(s):
    Ubiquitous glucose-6-phosphatase catalytic subunit-related protein

Gene names

Name:	G6pc3
Synonyms:	Ugrp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. May form with the glucose-6-phosphate transporter (SLC37A4/G6PT) a ubiquitously expressed complex responsible for glucose production through glycogenolysis and gluconeogenesis. Probably required for normal neutrophil function. Ref.5 Ref.7 Ref.8 Ref.9
Catalytic activity	D-glucose 6-phosphate + H2O = D-glucose + phosphate.
Enzyme regulation	Inhibited by vanadate By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Widely expressed. Highly expressed in heart, brain, kidney and testis and to a lower extent in lung, spleen, stomach, small intestine, skeletal muscle and uterus. Expressed in muscle, brain, thymus, lung, kidney, spleen and pancreas (at protein level). Ref.5 Ref.7 Ref.6
Disruption phenotype	Mice display reduced glucose-6-phosphate hydrolytic activity in the brain. No phenotypic difference was noted at birth but 4 months old female mice display growth retardation. Mutant mice exhibit a decreased plasma cholesterol concentration and an increased plasma glucagon concentration but no difference in blood glucose concentration (Ref.8). Mice display neutropenia and neutrophil dysfunctions. Ref.8 Ref.9
Sequence similarities	Belongs to the glucose-6-phosphatase family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glucose-6-phosphate transport Ref.7 Inferred from genetic interaction. Source: MGI
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose-6-phosphatase activity Ref.7 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Glucose-6-phosphatase 3		PRO_0000334513
Regions
	Topological domain	1 – 24	24	Lumenal Potential
	Transmembrane	25 – 45	21	Potential
	Topological domain	46 – 56	11	Cytoplasmic Potential
	Transmembrane	57 – 77	21	Potential
	Topological domain	78 – 108	31	Lumenal Potential
	Transmembrane	109 – 129	21	Potential
	Topological domain	130 – 138	9	Cytoplasmic Potential
	Transmembrane	139 – 159	21	Potential
	Topological domain	160 – 167	8	Lumenal Potential
	Transmembrane	168 – 186	19	Potential
	Topological domain	187 – 197	11	Cytoplasmic Potential
	Transmembrane	198 – 218	21	Potential
	Topological domain	219 – 254	36	Lumenal Potential
	Transmembrane	255 – 273	19	Potential
	Topological domain	274 – 283	10	Cytoplasmic Potential
	Transmembrane	284 – 304	21	Potential
	Topological domain	305 – 307	3	Lumenal Potential
	Transmembrane	308 – 328	21	Potential
	Topological domain	329 – 346	18	Cytoplasmic Potential
Sites
	Active site	114	1	Proton donor Potential
	Active site	167	1	Nucleophile By similarity
	Binding site	79	1	Substrate Potential
	Binding site	161	1	Substrate Potential
Experimental info
	Sequence conflict	136	1	R → Q in BAE38159. Ref.2
	Sequence conflict	207	1	M → L in AAO39164. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6NSQ9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 50CDA634BCABA014	FASTA	346	38,782
	10 20 30 40 50 60 MESTLSAGII MAEALQNRLP GLENMWLWVT FLGDPKNLFQ FCFPAAYYAS RRLGISVLWI 70 80 90 100 110 120 TFIAEWLNLV FKWFLFGDRP FWWVHESGYS TQTPIQIHQF PSSCETGPGS PSGHCMITGA 130 140 150 160 170 180 ALWPVMTAIS SQVASRSRSP WVRVIPGLAY CTFLLAVGLS RVFLLAHFPH QVLGGLIVGA 190 200 210 220 230 240 ALGWLMSPRV PMERELSFYG LTALALMLGA SLMYWTLFTL GLDLSWSINL ASKWCERPEW 250 260 270 280 290 300 VHMDSRPFAS LSRDSGSALG LGIALHTPCY AQIRRAHLGN GQKIACFVLA MGLLVFLEWL 310 320 330 340 GYPPQISLFY IFNFLKYTLW PCLVLALVPW VVHTLSDQEA PPIRSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a glucose-6-phosphatase catalytic subunit-related sequence expressed in rodent tissues." Middleditch C., Darakhshan F., Bonnefont J., Guionie O., Burchell A., Clottes E. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney.
[3]	The mouse genome sequencing consortium Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
[5]	"A potential new role for muscle in blood glucose homeostasis." Shieh J.-J., Pan C.-J., Mansfield B.C., Chou J.Y. J. Biol. Chem. 279:26215-26219(2004) [PubMed: 15087461] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"Identification and characterization of a cDNA and the gene encoding the mouse ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein." Boustead J.N., Martin C.C., Oeser J.K., Svitek C.A., Hunter S.I., Hutton J.C., O'Brien R.M. J. Mol. Endocrinol. 32:33-53(2004) [PubMed: 14765991] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[7]	"Brain contains a functional glucose-6-phosphatase complex capable of endogenous glucose production." Ghosh A., Cheung Y.Y., Mansfield B.C., Chou J.Y. J. Biol. Chem. 280:11114-11119(2005) [PubMed: 15661744] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[8]	"Deletion of the gene encoding the ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein (UGRP)/glucose-6-phosphatase catalytic subunit-beta results in lowered plasma cholesterol and elevated glucagon." Wang Y., Oeser J.K., Yang C., Sarkar S., Hackl S.I., Hasty A.H., McGuinness O.P., Paradee W., Hutton J.C., Powell D.R., O'Brien R.M. J. Biol. Chem. 281:39982-39989(2006) [PubMed: 17023421] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[9]	"Impaired neutrophil activity and increased susceptibility to bacterial infection in mice lacking glucose-6-phosphatase-beta." Cheung Y.Y., Kim S.Y., Yiu W.H., Pan C.-J., Jun H.-S., Ruef R.A., Lee E.J., Westphal H., Mansfield B.C., Chou J.Y. J. Clin. Invest. 117:784-793(2007) [PubMed: 17318259] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY186239 mRNA. Translation: AAO39164.1. AK165395 mRNA. Translation: BAE38159.1. AL954730 Genomic DNA. Translation: CAM25070.1. BC069959 mRNA. Translation: AAH69959.1.
IPI	IPI00421041.
RefSeq	NP_787949.2.
UniGene	Mm.22385
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000034793. Mus musculus. [Contig view]
GeneID	68401.
KEGG	mmu:68401.
Organism-specific databases
MGI	MGI:1915651. G6pc3.
Phylogenomic databases
HOVERGEN	Q6NSQ9.
OMA	Q6NSQ9. GIAVLWI.
Gene expression databases
ArrayExpress	Q6NSQ9.
Bgee	Q6NSQ9.
Family and domain databases
InterPro	IPR016275. Glucose-6-phosphatase. IPR000326. P_Acid_Pase_2/haloperoxidase. [Graphical view]
Pfam	PF01569. PAP2. 1 hit. [Graphical view]
PIRSF	PIRSF000905. Glucose-6-phosphatase. 1 hit.
SMART	SM00014. acidPPc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	327124.
SOURCE	Search...

Entry information

Entry name

G6PC3_MOUSE

Accession

Primary (citable) accession number: Q6NSQ9
Secondary accession number(s): Q3TND0, Q811R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)