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Protein

Adenylosuccinate synthetase isozyme 1

Gene

adssl1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).By similarity

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase isozyme 1 (adssl1), Adenylosuccinate synthetase (adssl), Adenylosuccinate synthetase isozyme 1 (adssl1), Adenylosuccinate synthetase isozyme 2 (adss), Adenylosuccinate synthetase (adssl1), Adenylosuccinate synthetase (adssl)
  2. Adenylosuccinate lyase (adsl), Adenylosuccinate lyase (adsl), Adenylosuccinate lyase (adsl), Adenylosuccinate lyase (adsl), Adenylosuccinate lyase (adsl), Adenylosuccinate lyase (adsl)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421Proton acceptorUniRule annotation
Metal bindingi42 – 421MagnesiumUniRule annotation
Binding sitei42 – 421SubstrateUniRule annotation
Metal bindingi69 – 691Magnesium; via carbonyl oxygenUniRule annotation
Active sitei70 – 701Proton donorUniRule annotation
Binding sitei162 – 1621IMPUniRule annotation
Binding sitei176 – 1761IMP; shared with dimeric partnerUniRule annotation
Binding sitei255 – 2551IMPUniRule annotation
Binding sitei270 – 2701IMPUniRule annotation
Binding sitei334 – 3341IMPUniRule annotation
Binding sitei336 – 3361GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi41 – 477GTPUniRule annotation
Nucleotide bindingi69 – 713GTPUniRule annotation
Nucleotide bindingi362 – 3643GTPUniRule annotation
Nucleotide bindingi444 – 4474GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • 'de novo' AMP biosynthetic process Source: GO_Central
  • IMP metabolic process Source: GO_Central
  • skeletal muscle fiber development Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetase isozyme 1UniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSase 1UniRule annotation
Short name:
AdSS 1UniRule annotation
Alternative name(s):
Adenylosuccinate synthetase, basic isozymeUniRule annotation
Adenylosuccinate synthetase, muscle isozymeUniRule annotation
Short name:
M-type adenylosuccinate synthetaseUniRule annotation
IMP--aspartate ligase 1UniRule annotation
Gene namesi
Name:adssl1
Synonyms:adss1
ORF Names:zgc:85738
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 17

Organism-specific databases

ZFINiZDB-GENE-040516-18. adssl1.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Adenylosuccinate synthetase isozyme 1PRO_0000398887Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6NSN5.
PRIDEiQ6NSN5.

PTM databases

iPTMnetiQ6NSN5.

Expressioni

Gene expression databases

BgeeiQ6NSN5.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi7955.ENSDARP00000046076.

Structurei

3D structure databases

ProteinModelPortaliQ6NSN5.
SMRiQ6NSN5. Positions 28-456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 454IMP bindingUniRule annotation
Regioni67 – 704IMP bindingUniRule annotation
Regioni330 – 3367Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1355. Eukaryota.
COG0104. LUCA.
GeneTreeiENSGT00390000015553.
HOGENOMiHOG000260959.
HOVERGENiHBG053768.
InParanoidiQ6NSN5.
KOiK01939.
OrthoDBiEOG7M6D76.
PhylomeDBiQ6NSN5.
TreeFamiTF300486.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth.
MF_03126. Adenylosucc_synth_vert_basic.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR033128. Adenylosuccin_syn_Lys_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027509. AdSS_1_vert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NSN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGWSQNDH RSYSNPPPVS GKRPRNDSGN KVTVVLGAQW GDEGKGKVVD
60 70 80 90 100
LLATESDIVG RCQGGNNAGH TVVVEEKEYD FHLLPSGIIN TKCTSFIGNG
110 120 130 140 150
VVIHLPGLFE EIDKNEKKGL KGWEKRLVIS DRAHIVFDFH QAVDGLQETQ
160 170 180 190 200
RQAQEGKNIG TTKKGIGPTY ACKASRTGLR ICDLMADFNE FSTRVKNLVQ
210 220 230 240 250
QYQSMYPTLK VDVESELKKL KEYAERIRPL VRDGVYFMYD AIHGPQKKIL
260 270 280 290 300
VEGANAALLD IDFGTYPFVT SSNCTVGGVC TGLGIPPANI GDVYGVSKAY
310 320 330 340 350
TTRVGIGAFP TEQLNAVGEL LQTRGHEVGV TTGRKRRCGW LDLVILKYAH
360 370 380 390 400
MINGFTAIAL TKLDILDVLD EIKVGVAYKI NGKRIPHFPA NLEVLQKVEV
410 420 430 440 450
EYETFPGWKS DTSAARKWGD LPAKAQNYIR FVENHIGVPI KWVGVGKSRE

CMIQMF
Length:456
Mass (Da):50,374
Last modified:July 5, 2004 - v1
Checksum:i800011BE05689071
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC070009 mRNA. Translation: AAH70009.1.
RefSeqiNP_999985.1. NM_214820.1.
UniGeneiDr.106150.

Genome annotation databases

EnsembliENSDART00000162819; ENSDARP00000141430; ENSDARG00000099517.
GeneIDi407989.
KEGGidre:407989.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC070009 mRNA. Translation: AAH70009.1.
RefSeqiNP_999985.1. NM_214820.1.
UniGeneiDr.106150.

3D structure databases

ProteinModelPortaliQ6NSN5.
SMRiQ6NSN5. Positions 28-456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000046076.

PTM databases

iPTMnetiQ6NSN5.

Proteomic databases

PaxDbiQ6NSN5.
PRIDEiQ6NSN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000162819; ENSDARP00000141430; ENSDARG00000099517.
GeneIDi407989.
KEGGidre:407989.

Organism-specific databases

CTDi122622.
ZFINiZDB-GENE-040516-18. adssl1.

Phylogenomic databases

eggNOGiKOG1355. Eukaryota.
COG0104. LUCA.
GeneTreeiENSGT00390000015553.
HOGENOMiHOG000260959.
HOVERGENiHBG053768.
InParanoidiQ6NSN5.
KOiK01939.
OrthoDBiEOG7M6D76.
PhylomeDBiQ6NSN5.
TreeFamiTF300486.

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.
ReactomeiR-DRE-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiQ6NSN5.

Gene expression databases

BgeeiQ6NSN5.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth.
MF_03126. Adenylosucc_synth_vert_basic.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR033128. Adenylosuccin_syn_Lys_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027509. AdSS_1_vert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  2. "Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo."
    Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J., Slijper M., Heck A.J.R.
    J. Proteome Res. 7:1555-1564(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiPURA1_DANRE
AccessioniPrimary (citable) accession number: Q6NSN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.