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Q6NSN2 (AOF_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amine oxidase [flavin-containing]

EC=1.4.3.4
Alternative name(s):
Monoamine oxidase
Short name=MAO
Short name=Z-MAO
Gene names
Name:mao
ORF Names:zgc:85761
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length522 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. Oxidizes both 5-hydroxytryptamine (5-HT) and beta-phenylethylamine (PEA). Ref.1 Ref.2 UniProtKB P21397

Catalytic activity

RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2. Ref.1 Ref.2

Cofactor

FAD.

Enzyme regulation

Inhibited by both clorgyline (selective MAOA inhibitor) and deprenyl (selective MAOB inhibitor). Ref.1 Ref.2

Subunit structure

Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity. UniProtKB P21397

Subcellular location

Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.

Tissue specificity

Strongest expression in brain and intestine, followed by liver, heart and gill. Little expression in spleen, eye or muscle. In brain, highest activity in noradrenergic and serotonergic cell groups and those of the habenulointerpeduncular pathway; moderate levels in dopaminergic cell clusters. Ref.2

Miscellaneous

There appears to be only one form of this enzyme in zebrafish, whereas in mammals two forms (MAOA and MAOB) exist. Ref.1 Ref.2

Sequence similarities

Belongs to the flavin monoamine oxidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 522522Amine oxidase [flavin-containing]
PRO_0000283788

Regions

Topological domain1 – 492492Cytoplasmic Potential
Transmembrane493 – 51321Helical; Anchor for type IV membrane protein; Potential
Topological domain514 – 5229Mitochondrial intermembrane Potential

Sites

Site1571Important for catalytic activity By similarity UniProtKB P27338
Site3661Important for catalytic activity By similarity UniProtKB P27338
Site3831Important for catalytic activity By similarity UniProtKB P27338

Amino acid modifications

Modified residue3981S-8alpha-FAD cysteine By similarity UniProtKB P27338

Experimental info

Sequence conflict223 – 2242AR → QK in AAO16681. Ref.1
Sequence conflict2341R → K in AAO16681. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6NSN2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 8FC58B2A13BFE15C

FASTA52258,765
        10         20         30         40         50         60 
MTANAYDVIV IGGGISGLSA AKLLVDSGLN PVVLEARSRV GGRTYTVQNK ETKWVDLGGA 

        70         80         90        100        110        120 
YIGPTQNRIL RIAKQYGVKT YKVNEEESLV HYVKGKSYPF KGPFPPMWNP FAYMDYNNLW 

       130        140        150        160        170        180 
RTMDKMGMEI PKEAPWRAPH AEEWDKMTMQ QLFDKICWTR SARRFATLFV NVNVTSEPHE 

       190        200        210        220        230        240 
VSALWFLWYV KQCGGTMRIF STTNGGQERK FAGGANQISE GMARELGDRV KLSRAVCSID 

       250        260        270        280        290        300 
QTGDLVEVRT VNEEVYKAKY VILAIPPGLN LKIHFNPELP PLRNQLIHRV PMGSVIKCMV 

       310        320        330        340        350        360 
YYKENFWRKK GYCGSMVIEE EDAPIGLTLD DTKPDGSVPA IMGFILARKS RKLANLTRDE 

       370        380        390        400        410        420 
RKRRICEIYA RVLGSEEALY PVHYEEKNWC EEEYSGGCYT AYFPPGIMTQ FGRVLREPVG 

       430        440        450        460        470        480 
RLYFAGTETA TEWSGYMEGA VQAGERASRE VMCAMGKLHA SQIWQSEPES MDVPARPFVT 

       490        500        510        520 
TFWERNLPSV GGFLKFMGVS SFLAAATAAG LVACKKGLLP RC 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of monoamine oxidase in zebrafish (Danio rerio)."
Setini A., Pierucci F., Senatori O., Nicotra A.
Comp. Biochem. Physiol. 140B:153-161(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, 3D-STRUCTURE MODELING.
Tissue: Liver.
[2]"Distinct structure and activity of monoamine oxidase in the brain of zebrafish (Danio rerio)."
Anichtchik O., Sallinen V., Peitsaro N., Panula P.
J. Comp. Neurol. 498:593-610(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY.
Tissue: Brain.
[3]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY185211 mRNA. Translation: AAO16681.3.
BC070013 mRNA. Translation: AAH70013.1.
RefSeqNP_997992.2. NM_212827.2.
UniGeneDr.77508.

3D structure databases

ProteinModelPortalQ6NSN2.
SMRQ6NSN2. Positions 6-504.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ6NSN2.
ChEMBLCHEMBL1681610.

Proteomic databases

PRIDEQ6NSN2.

Protocols and materials databases

DNASU404730.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000028225; ENSDARP00000032834; ENSDARG00000023712.
GeneID404730.
KEGGdre:404730.

Organism-specific databases

CTD404730.
ZFINZDB-GENE-040329-3. mao.

Phylogenomic databases

eggNOGCOG1231.
GeneTreeENSGT00730000110903.
HOGENOMHOG000221615.
HOVERGENHBG004255.
InParanoidQ6NSN2.
KOK00274.
OMAFSTEPRE.
OrthoDBEOG7K6PTP.
PhylomeDBQ6NSN2.
TreeFamTF313314.

Enzyme and pathway databases

BRENDA1.4.3.4. 1832.

Gene expression databases

BgeeQ6NSN2.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSPR00757. AMINEOXDASEF.
ProtoNetSearch...

Other

NextBio20817705.
PROQ6NSN2.

Entry information

Entry nameAOF_DANRE
AccessionPrimary (citable) accession number: Q6NSN2
Secondary accession number(s): Q804D6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families