Reviewed, UniProtKB/Swiss-Prot Q6NSN2 (AOF_DANRE)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing]
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase
    Z-MAO
      Short name=MAO

Gene names

Name:	mao
ORF Names:	zgc:85761

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. Oxidizes both 5-hydroxytryptamine (5-HT) and beta-phenylethylamine (PEA).
Catalytic activity	RCH(2)NHR' + H(2)O + O(2) = RCHO + R'NH(2) + H(2)O(2).
Cofactor	FAD.
Enzyme regulation	Inhibited by both clorgyline (selective MAOA inhibitor) and deprenyl (selective MAOB inhibitor).
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
Tissue specificity	Strongest expression in brain and intestine, followed by liver, heart and gill. Little expression in spleen, eye or muscle. In brain, highest activity in noradrenergic and serotonergic cell groups and those of the habenulointerpeduncular pathway; moderate levels in dopaminergic cell clusters.
Miscellaneous	There appears to be only one form of this enzyme in zebrafish, whereas in mammals two forms (MAOA and MAOB) exist.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Biological process	Catecholamine metabolism Neurotransmitter degradation
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	biogenic amine catabolic process Ref.1 Ref.2 Inferred from direct assay. Source: UniProtKB catecholamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amine oxidase activity Ref.1 Ref.2 Inferred from direct assay. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Amine oxidase [flavin-containing]		PRO_0000283788
Regions
	Topological domain	1 – 492	492	Cytoplasmic Potential
	Transmembrane	493 – 513	21	Anchor for type IV membrane protein Potential
	Topological domain	514 – 522	9	Mitochondrial intermembrane Potential
Sites
	Site	157	1	Important for catalytic activity By similarity
	Site	366	1	Important for catalytic activity By similarity
	Site	383	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	398	1	S-8alpha-FAD cysteine By similarity
Experimental info
	Sequence conflict	223 – 224	2	AR → QK in AAO16681. Ref.1
	Sequence conflict	234	1	R → K in AAO16681. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6NSN2-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8FC58B2A13BFE15C	FASTA	522	58,765
	10 20 30 40 50 60 MTANAYDVIV IGGGISGLSA AKLLVDSGLN PVVLEARSRV GGRTYTVQNK ETKWVDLGGA 70 80 90 100 110 120 YIGPTQNRIL RIAKQYGVKT YKVNEEESLV HYVKGKSYPF KGPFPPMWNP FAYMDYNNLW 130 140 150 160 170 180 RTMDKMGMEI PKEAPWRAPH AEEWDKMTMQ QLFDKICWTR SARRFATLFV NVNVTSEPHE 190 200 210 220 230 240 VSALWFLWYV KQCGGTMRIF STTNGGQERK FAGGANQISE GMARELGDRV KLSRAVCSID 250 260 270 280 290 300 QTGDLVEVRT VNEEVYKAKY VILAIPPGLN LKIHFNPELP PLRNQLIHRV PMGSVIKCMV 310 320 330 340 350 360 YYKENFWRKK GYCGSMVIEE EDAPIGLTLD DTKPDGSVPA IMGFILARKS RKLANLTRDE 370 380 390 400 410 420 RKRRICEIYA RVLGSEEALY PVHYEEKNWC EEEYSGGCYT AYFPPGIMTQ FGRVLREPVG 430 440 450 460 470 480 RLYFAGTETA TEWSGYMEGA VQAGERASRE VMCAMGKLHA SQIWQSEPES MDVPARPFVT 490 500 510 520 TFWERNLPSV GGFLKFMGVS SFLAAATAAG LVACKKGLLP RC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of monoamine oxidase in zebrafish (Danio rerio)." Setini A., Pierucci F., Senatori O., Nicotra A. Comp. Biochem. Physiol. 140B:153-161(2005) [PubMed: 15621520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, 3D-STRUCTURE MODELING. Tissue: Liver.
[2]	"Distinct structure and activity of monoamine oxidase in the brain of zebrafish (Danio rerio)." Anichtchik O., Sallinen V., Peitsaro N., Panula P. J. Comp. Neurol. 498:593-610(2006) [PubMed: 16917825] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY. Tissue: Brain.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY185211 mRNA. Translation: AAO16681.3. BC070013 mRNA. Translation: AAH70013.1.
RefSeq	NP_997992.2.
UniGene	Dr.77508
3D structure databases
SMR	Q6NSN2. Positions 4-501.
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000023712. Danio rerio. [Contig view]
GeneID	404730.
KEGG	dre:404730.
Organism-specific databases
ZFIN	ZDB-GENE-040329-3. mao.
Phylogenomic databases
HOVERGEN	Q6NSN2.
Gene expression databases
ArrayExpress	Q6NSN2.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...

Entry information

Entry name

AOF_DANRE

Accession

Primary (citable) accession number: Q6NSN2
Secondary accession number(s): Q804D6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project