ID   Q6NSI7_HUMAN            Unreviewed;      1091 AA.
AC   Q6NSI7;
DT   05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT   17-APR-2007, sequence version 2.
DT   24-JAN-2024, entry version 108.
DE   SubName: Full=Solute carrier family 12 (Potassium/chloride transporters), member 6 {ECO:0000313|EMBL:AAH70107.2};
GN   Name=SLC12A6 {ECO:0000313|EMBL:AAH70107.2};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|EMBL:AAH70107.2};
RN   [1] {ECO:0000313|EMBL:AAH70107.2}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis {ECO:0000313|EMBL:AAH70107.2};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RA   Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H.,
RA   Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M.,
RA   Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.,
RA   Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F.,
RA   Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T.,
RA   Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F.,
RA   Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E.,
RA   Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y.,
RA   Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E.,
RA   Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y.,
RA   Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W.,
RA   Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J.,
RA   Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W.,
RA   Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J.,
RA   Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I.,
RA   Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U.,
RA   Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A.,
RA   Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chloride(in) + K(+)(in) = chloride(out) + K(+)(out);
CC         Xref=Rhea:RHEA:72427, ChEBI:CHEBI:17996, ChEBI:CHEBI:29103;
CC         Evidence={ECO:0000256|ARBA:ARBA00034453};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC       {ECO:0000256|ARBA:ARBA00010593}.
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DR   EMBL; BC070107; AAH70107.2; -; mRNA.
DR   RefSeq; NP_001035960.1; NM_001042495.1.
DR   AlphaFoldDB; Q6NSI7; -.
DR   PeptideAtlas; Q6NSI7; -.
DR   DNASU; 9990; -.
DR   GeneID; 9990; -.
DR   CTD; 9990; -.
DR   PharmGKB; PA35808; -.
DR   OrthoDB; 5490251at2759; -.
DR   BioGRID-ORCS; 9990; 12 hits in 1163 CRISPR screens.
DR   ChiTaRS; SLC12A6; human.
DR   GenomeRNAi; 9990; -.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015379; F:potassium:chloride symporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR   InterPro; IPR004841; AA-permease/SLC12A_dom.
DR   InterPro; IPR000076; KCL_cotranspt.
DR   InterPro; IPR018491; SLC12_C.
DR   InterPro; IPR004842; SLC12A_fam.
DR   NCBIfam; TIGR00930; 2a30; 1.
DR   PANTHER; PTHR11827:SF66; SOLUTE CARRIER FAMILY 12 MEMBER 6; 1.
DR   PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR   Pfam; PF00324; AA_permease; 2.
DR   Pfam; PF03522; SLC12; 2.
DR   PRINTS; PR01081; KCLTRNSPORT.
DR   Genevisible; Q6NSI7; HS.
PE   2: Evidence at transcript level;
KW   Chloride {ECO:0000256|ARBA:ARBA00023214};
KW   Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Potassium {ECO:0000256|ARBA:ARBA00022958};
KW   Potassium transport {ECO:0000256|ARBA:ARBA00022538};
KW   Symport {ECO:0000256|ARBA:ARBA00022847};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022847}.
FT   TRANSMEM        127..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        153..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        213..238
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        259..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        284..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        417..437
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        457..483
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        562..580
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        586..607
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        619..636
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          135..304
FT                   /note="Amino acid permease/ SLC12A"
FT                   /evidence="ECO:0000259|Pfam:PF00324"
FT   DOMAIN          423..701
FT                   /note="Amino acid permease/ SLC12A"
FT                   /evidence="ECO:0000259|Pfam:PF00324"
FT   DOMAIN          715..833
FT                   /note="SLC12A transporter C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03522"
FT   DOMAIN          847..1091
FT                   /note="SLC12A transporter C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03522"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          101..122
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        104..118
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1091 AA;  121109 MW;  EC5F6EE6E9E6957B CRC64;
     MSEMSGATTS LATVALDPPS DRTSHPQDVI EDLSQNSITG EHSQLLDDGH KKARNAYLNN
     SNYEEGDEYF DKNLALFEEE MDTRPKVSSL LNRMANYTNL TQGAKEHEEA ENITEGKKKP
     TKTPQMGTFM GVYPPCLQNI FGVILFLRLT WVVGTAGVLQ AFAIVLICCC CTMLTAISMS
     AIATNGVVPA GGSYFMISRA LGPEFGGAVG LCFYLGTTFA AAMYILGAIE IFLVYIVPRA
     AIFHSDDALK ESAAMLNNMR VYGTAFLVLM VLVVFIGVRY VNKFASLFLA CVIVSILAIY
     AGAIKSSFAP PHFPVCMLGN RTLSSRHIDV CSKTKEINNM TVPSKLWGFF CNSSQFFNAT
     CDEYFVHNNV TSIQGIPGLA SGIITENLWS NYLPKGEIIE KPSAKSSDVL GSLNHEYVLV
     DITTSFTLLV GIFFPSVTGI MAGSNRSGDL KDAQKSIPIG TILAILTTSF VYLSNVVLFG
     ACIEGVVLRD KFGDAVKGNL VVGTLSWPSP WVIVIGSFFS TCGAGLQSLT GAPRLLQAIA
     KDNIIPFLRV FGHSKANGEP TWALLLTAAI AELGILIASL DLVAPILSMF FLMCYLFVNL
     ACALQTLLRT PNWRPRFRYY HWALSFMGMS ICLALMFISS WYYAIVAMVI AGMIYKYIEY
     QGAEKEWGDG IRGLSLSAAR FALLRLEEGP PHTKNWRPQL LVLLKLDEDL HVKHPRLLTF
     ASQLKAGKGL TIVGSVIVGN FLENYGEALA AEQTIKHLME AEKVKGFCQL VVAAKLREGI
     SHLIQSCGLG GMKHNTVVMG WPNGWRQSED ARAWKTFIGT VRVTTAAHLA LLVAKNISFF
     PSNVEQFSEG NIDVWWIVHD GGMLMLLPFL LKQHKVWRKC SIRIFTVAQL EDNSIQMKKD
     LATFLYHLRI EAEVEVVEMH DSDISAYTYE RTLMMEQRSQ MLRHMRLSKT ERDREAQLVK
     DRNSMLRLTS IGSDEDEETE TYQEKVHMTW TKDKYMASRG QKAKSMEGFQ DLLNMRPDQS
     NVRRMHTAVK LNEVIVNKSH EAKLVLLNMP GPPRNPEGDE NYMEFLEVLT EGLERVLLVR
     GGGSEVITIY S
//