Reviewed, UniProtKB/Swiss-Prot Q6NS52 (DGKB_MOUSE)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase beta
      Short name=DAG kinase beta
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase beta
      Short name=DGK-beta

Gene names

Name:	Dgkb
Synonyms:	Kiaa0718

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	802 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Exhibits high phosphorylation activity for long-chain diacylglycerols By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Enzyme regulation	Stimulated by phosphatidylserine By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 EF-hand domains. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from direct assay. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6NS52-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6NS52-2)

The sequence of this isoform differs from the canonical sequence as follows:
     50-56: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 802	802	Diacylglycerol kinase beta		PRO_0000264623
Regions
	Domain	148 – 183	36	EF-hand 1
	Domain	193 – 228	36	EF-hand 2
	Domain	432 – 566	135	DAGKc
	Calcium binding	161 – 172	12	1 Potential
	Calcium binding	206 – 217	12	2 Potential
	Zinc finger	243 – 293	51	Phorbol-ester/DAG-type 1
	Zinc finger	308 – 357	50	Phorbol-ester/DAG-type 2
Natural variations
	Alternative sequence	50 – 56	7	Missing in isoform 2.		VSP_021900

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 2995ECF41057DEF3	FASTA	802	90,272
	10 20 30 40 50 60 MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK QDILNQTIDF 70 80 90 100 110 120 EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS KPALLSGGLR MNKGAITPPR 130 140 150 160 170 180 SSPANTCSPE VIHLKDIVCY LSLLERGRPE DKLEFMFRLY DTDGNGFLDS SELENIIGQM 190 200 210 220 230 240 MHVAEYLEWD VTELNPILHE MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD 250 260 270 280 290 300 DGQHVWRLKH FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCVARAP PSCIKTYVKS 310 320 330 340 350 360 KKNTDVMHHY WVEGNCPTKC DKCHKTVKCY QGLTGLHCVW CQTTLHNKCA SHLKPECDCG 370 380 390 400 410 420 PLKDHILPPT TICPVVLTMP SAGASVPEER QSTAKKEKSS SQQPNKATDK NKMQRANSVT 430 440 450 460 470 480 MDGQGLQITP VPGTHPLLVF VNPKSGGKQG ERIYRKFQYL LNPRQVYSLS GNGPMPGLHF 490 500 510 520 530 540 FRDVPDFRVL ACGGDGTVGW ILDCIEKANV VKHPPVAILP LGTGNDLARC LRWGGGYEGE 550 560 570 580 590 600 NLMKILKDIE SSTEIMLDRW KFEVTPNDKD EKGDPVPYSI INNYFSIGVD ASIAHRFHIM 610 620 630 640 650 660 REKHPEKFNS RMKNKFWYFE FGTSETFSAT CKKLHESVEI ECDGVQIDLI NISLEGIAIL 670 680 690 700 710 720 NIPSMHGGSN LWGESKKKRS HRRIEKKGSD KRPTLTDAKE LKFASQDLSD QLLEVVGLEG 730 740 750 760 770 780 AMEMGQIYTG LKSAGRRLAQ CSSVVIRTSK SLPMQIDGEP WMQTPCTIKI THKNQAPMLM 790 800 GPPPKTGLFC SLIKRTRNRS KE
	Isoform 2. Checksum: D4762491529AB631	FASTA	795	89,432
	10 20 30 40 50 60 MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGT IDFEGFKLFM 70 80 90 100 110 120 KTFLEAELPD DFTAHLFMSF SNKFPHSSPN VKSKPALLSG GLRMNKGAIT PPRSSPANTC 130 140 150 160 170 180 SPEVIHLKDI VCYLSLLERG RPEDKLEFMF RLYDTDGNGF LDSSELENII GQMMHVAEYL 190 200 210 220 230 240 EWDVTELNPI LHEMMEEIDY DRDGTVSLEE WIQGGMTTIP LLVLLGLENN VKDDGQHVWR 250 260 270 280 290 300 LKHFNKPAYC NLCLNMLIGV GKQGLCCSFC KYTVHERCVA RAPPSCIKTY VKSKKNTDVM 310 320 330 340 350 360 HHYWVEGNCP TKCDKCHKTV KCYQGLTGLH CVWCQTTLHN KCASHLKPEC DCGPLKDHIL 370 380 390 400 410 420 PPTTICPVVL TMPSAGASVP EERQSTAKKE KSSSQQPNKA TDKNKMQRAN SVTMDGQGLQ 430 440 450 460 470 480 ITPVPGTHPL LVFVNPKSGG KQGERIYRKF QYLLNPRQVY SLSGNGPMPG LHFFRDVPDF 490 500 510 520 530 540 RVLACGGDGT VGWILDCIEK ANVVKHPPVA ILPLGTGNDL ARCLRWGGGY EGENLMKILK 550 560 570 580 590 600 DIESSTEIML DRWKFEVTPN DKDEKGDPVP YSIINNYFSI GVDASIAHRF HIMREKHPEK 610 620 630 640 650 660 FNSRMKNKFW YFEFGTSETF SATCKKLHES VEIECDGVQI DLINISLEGI AILNIPSMHG 670 680 690 700 710 720 GSNLWGESKK KRSHRRIEKK GSDKRPTLTD AKELKFASQD LSDQLLEVVG LEGAMEMGQI 730 740 750 760 770 780 YTGLKSAGRR LAQCSSVVIR TSKSLPMQID GEPWMQTPCT IKITHKNQAP MLMGPPPKTG 790 LFCSLIKRTR NRSKE

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Brain cortex.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Eye.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed: 12693553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 34-802 (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK139408 mRNA. Translation: BAE23997.1. BC070461 mRNA. Translation: AAH70461.1. AK122355 mRNA. Translation: BAC65637.1.
IPI	IPI00421000. IPI00816974.
RefSeq	NP_848796.2.
UniGene	Mm.126525
3D structure databases
SMR	Q6NS52. Positions 5-156, 9-238, 156-296, 242-311, 302-362.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6NS52.
PTM databases
PhosphoSite	Q6NS52.
Proteomic databases
PRIDE	Q6NS52.
Genome annotation databases
Ensembl	ENSMUST00000040500; ENSMUSP00000037900; ENSMUSG00000036095; Mus musculus. [Genome view] ENSMUST00000072865; ENSMUSP00000072642; ENSMUSG00000036095; Mus musculus. [Genome view]
GeneID	217480.
KEGG	mmu:217480.
UCSC	uc007nkj.1. mouse. uc007nkn.1. mouse.
Organism-specific databases
CTD	217480.
MGI	MGI:2442474. Dgkb.
Rouge	Search...
Phylogenomic databases
HOGENOM	HBG315056.
HOVERGEN	Q6NS52.
InParanoid	Q6NS52.
OMA	NQAPMLM.
OrthoDB	EOG9VQD83.
Enzyme and pathway databases
BRENDA	2.7.1.107. 244.
Gene expression databases
ArrayExpress	Q6NS52.
Bgee	Q6NS52.
CleanEx	MM_DGKB.
Genevestigator	Q6NS52.
GermOnline	ENSMUSG00000036095. Mus musculus.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 2 hits. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	375875.
SOURCE	Search...

Entry information

Entry name

DGKB_MOUSE

Accession

Primary (citable) accession number: Q6NS52
Secondary accession number(s): Q80TT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	January 19, 2010
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)