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Protein

Diacylglycerol kinase beta

Gene

Dgkb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits high phosphorylation activity for long-chain diacylglycerols.By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by phosphatidylserine.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi161 – 1721PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi206 – 2172PROSITE-ProRule annotationAdd BLAST12
Zinc fingeri243 – 293Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri308 – 357Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: InterPro
  • diacylglycerol kinase activity Source: MGI
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-114508 Effects of PIP2 hydrolysis

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase beta (EC:2.7.1.107)
Short name:
DAG kinase beta
Alternative name(s):
Diglyceride kinase beta
Short name:
DGK-beta
Gene namesi
Name:Dgkb
Synonyms:Kiaa0718
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2442474 Dgkb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002646231 – 802Diacylglycerol kinase betaAdd BLAST802

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphothreonineCombined sources1
Modified residuei418PhosphoserineCombined sources1
Modified residuei791PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6NS52
PeptideAtlasiQ6NS52
PRIDEiQ6NS52

PTM databases

iPTMnetiQ6NS52
PhosphoSitePlusiQ6NS52
SwissPalmiQ6NS52

Expressioni

Gene expression databases

BgeeiENSMUSG00000036095
CleanExiMM_DGKB
ExpressionAtlasiQ6NS52 baseline and differential
GenevisibleiQ6NS52 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037900

Structurei

3D structure databases

ProteinModelPortaliQ6NS52
SMRiQ6NS52
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 183EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini193 – 228EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini432 – 566DAGKcPROSITE-ProRule annotationAdd BLAST135

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri243 – 293Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri308 – 357Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
GeneTreeiENSGT00760000119050
HOGENOMiHOG000252931
HOVERGENiHBG051345
InParanoidiQ6NS52
KOiK00901
OMAiTDKNKMQ
OrthoDBiEOG091G04A5
PhylomeDBiQ6NS52
TreeFamiTF313104

Family and domain databases

CDDicd00029 C1, 2 hits
cd00051 EFh, 1 hit
Gene3Di1.10.238.110, 2 hits
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR029477 DAG_kinase_typeI_N
IPR037607 DGK
IPR038199 DGK_typeI_N_sf
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF14513 DAG_kinase_N, 2 hits
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF111331 SSF111331, 2 hits
SSF47473 SSF47473, 3 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NS52-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK
60 70 80 90 100
QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS
110 120 130 140 150
KPALLSGGLR MNKGAITPPR SSPANTCSPE VIHLKDIVCY LSLLERGRPE
160 170 180 190 200
DKLEFMFRLY DTDGNGFLDS SELENIIGQM MHVAEYLEWD VTELNPILHE
210 220 230 240 250
MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD DGQHVWRLKH
260 270 280 290 300
FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCVARAP PSCIKTYVKS
310 320 330 340 350
KKNTDVMHHY WVEGNCPTKC DKCHKTVKCY QGLTGLHCVW CQTTLHNKCA
360 370 380 390 400
SHLKPECDCG PLKDHILPPT TICPVVLTMP SAGASVPEER QSTAKKEKSS
410 420 430 440 450
SQQPNKATDK NKMQRANSVT MDGQGLQITP VPGTHPLLVF VNPKSGGKQG
460 470 480 490 500
ERIYRKFQYL LNPRQVYSLS GNGPMPGLHF FRDVPDFRVL ACGGDGTVGW
510 520 530 540 550
ILDCIEKANV VKHPPVAILP LGTGNDLARC LRWGGGYEGE NLMKILKDIE
560 570 580 590 600
SSTEIMLDRW KFEVTPNDKD EKGDPVPYSI INNYFSIGVD ASIAHRFHIM
610 620 630 640 650
REKHPEKFNS RMKNKFWYFE FGTSETFSAT CKKLHESVEI ECDGVQIDLI
660 670 680 690 700
NISLEGIAIL NIPSMHGGSN LWGESKKKRS HRRIEKKGSD KRPTLTDAKE
710 720 730 740 750
LKFASQDLSD QLLEVVGLEG AMEMGQIYTG LKSAGRRLAQ CSSVVIRTSK
760 770 780 790 800
SLPMQIDGEP WMQTPCTIKI THKNQAPMLM GPPPKTGLFC SLIKRTRNRS

KE
Length:802
Mass (Da):90,272
Last modified:December 12, 2006 - v2
Checksum:i2995ECF41057DEF3
GO
Isoform 2 (identifier: Q6NS52-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-56: Missing.

Show »
Length:795
Mass (Da):89,432
Checksum:iD4762491529AB631
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02190050 – 56Missing in isoform 2. 2 Publications7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139408 mRNA Translation: BAE23997.1
BC070461 mRNA Translation: AAH70461.1
AK122355 mRNA Translation: BAC65637.1
CCDSiCCDS25889.1 [Q6NS52-2]
RefSeqiNP_848796.2, NM_178681.4 [Q6NS52-2]
XP_006515132.1, XM_006515069.2 [Q6NS52-1]
XP_006515135.1, XM_006515072.3 [Q6NS52-2]
XP_017170508.1, XM_017315019.1 [Q6NS52-1]
UniGeneiMm.126525

Genome annotation databases

EnsembliENSMUST00000040500; ENSMUSP00000037900; ENSMUSG00000036095 [Q6NS52-2]
ENSMUST00000220990; ENSMUSP00000152378; ENSMUSG00000036095 [Q6NS52-2]
GeneIDi217480
KEGGimmu:217480
UCSCiuc007nkj.1 mouse [Q6NS52-2]
uc007nkn.1 mouse [Q6NS52-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDGKB_MOUSE
AccessioniPrimary (citable) accession number: Q6NS52
Secondary accession number(s): Q80TT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: June 20, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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