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Q6NS52

- DGKB_MOUSE

UniProt

Q6NS52 - DGKB_MOUSE

Protein

Diacylglycerol kinase beta

Gene

Dgkb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Exhibits high phosphorylation activity for long-chain diacylglycerols.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Enzyme regulationi

    Stimulated by phosphatidylserine.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi161 – 172121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi206 – 217122PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri243 – 29351Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri308 – 35750Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. diacylglycerol kinase activity Source: MGI
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_219232. Effects of PIP2 hydrolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase beta (EC:2.7.1.107)
    Short name:
    DAG kinase beta
    Alternative name(s):
    Diglyceride kinase beta
    Short name:
    DGK-beta
    Gene namesi
    Name:Dgkb
    Synonyms:Kiaa0718
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:2442474. Dgkb.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 802802Diacylglycerol kinase betaPRO_0000264623Add
    BLAST

    Proteomic databases

    PaxDbiQ6NS52.
    PRIDEiQ6NS52.

    PTM databases

    PhosphoSiteiQ6NS52.

    Expressioni

    Gene expression databases

    ArrayExpressiQ6NS52.
    BgeeiQ6NS52.
    CleanExiMM_DGKB.
    GenevestigatoriQ6NS52.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6NS52.
    SMRiQ6NS52. Positions 10-232, 244-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini148 – 18336EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini193 – 22836EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini432 – 566135DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri243 – 29351Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri308 – 35750Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    GeneTreeiENSGT00750000117569.
    HOGENOMiHOG000252931.
    HOVERGENiHBG051345.
    InParanoidiQ6NS52.
    KOiK00901.
    OMAiAQCSEVI.
    OrthoDBiEOG75XGK8.
    PhylomeDBiQ6NS52.
    TreeFamiTF313104.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6NS52-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK    50
    QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS 100
    KPALLSGGLR MNKGAITPPR SSPANTCSPE VIHLKDIVCY LSLLERGRPE 150
    DKLEFMFRLY DTDGNGFLDS SELENIIGQM MHVAEYLEWD VTELNPILHE 200
    MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD DGQHVWRLKH 250
    FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCVARAP PSCIKTYVKS 300
    KKNTDVMHHY WVEGNCPTKC DKCHKTVKCY QGLTGLHCVW CQTTLHNKCA 350
    SHLKPECDCG PLKDHILPPT TICPVVLTMP SAGASVPEER QSTAKKEKSS 400
    SQQPNKATDK NKMQRANSVT MDGQGLQITP VPGTHPLLVF VNPKSGGKQG 450
    ERIYRKFQYL LNPRQVYSLS GNGPMPGLHF FRDVPDFRVL ACGGDGTVGW 500
    ILDCIEKANV VKHPPVAILP LGTGNDLARC LRWGGGYEGE NLMKILKDIE 550
    SSTEIMLDRW KFEVTPNDKD EKGDPVPYSI INNYFSIGVD ASIAHRFHIM 600
    REKHPEKFNS RMKNKFWYFE FGTSETFSAT CKKLHESVEI ECDGVQIDLI 650
    NISLEGIAIL NIPSMHGGSN LWGESKKKRS HRRIEKKGSD KRPTLTDAKE 700
    LKFASQDLSD QLLEVVGLEG AMEMGQIYTG LKSAGRRLAQ CSSVVIRTSK 750
    SLPMQIDGEP WMQTPCTIKI THKNQAPMLM GPPPKTGLFC SLIKRTRNRS 800
    KE 802
    Length:802
    Mass (Da):90,272
    Last modified:December 12, 2006 - v2
    Checksum:i2995ECF41057DEF3
    GO
    Isoform 2 (identifier: Q6NS52-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         50-56: Missing.

    Show »
    Length:795
    Mass (Da):89,432
    Checksum:iD4762491529AB631
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei50 – 567Missing in isoform 2. 2 PublicationsVSP_021900

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK139408 mRNA. Translation: BAE23997.1.
    BC070461 mRNA. Translation: AAH70461.1.
    AK122355 mRNA. Translation: BAC65637.1.
    CCDSiCCDS25889.1. [Q6NS52-2]
    RefSeqiNP_848796.2. NM_178681.4. [Q6NS52-2]
    XP_006515132.1. XM_006515069.1. [Q6NS52-1]
    XP_006515135.1. XM_006515072.1. [Q6NS52-2]
    UniGeneiMm.126525.

    Genome annotation databases

    EnsembliENSMUST00000040500; ENSMUSP00000037900; ENSMUSG00000036095. [Q6NS52-2]
    GeneIDi217480.
    KEGGimmu:217480.
    UCSCiuc007nkj.1. mouse. [Q6NS52-2]
    uc007nkn.1. mouse. [Q6NS52-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK139408 mRNA. Translation: BAE23997.1 .
    BC070461 mRNA. Translation: AAH70461.1 .
    AK122355 mRNA. Translation: BAC65637.1 .
    CCDSi CCDS25889.1. [Q6NS52-2 ]
    RefSeqi NP_848796.2. NM_178681.4. [Q6NS52-2 ]
    XP_006515132.1. XM_006515069.1. [Q6NS52-1 ]
    XP_006515135.1. XM_006515072.1. [Q6NS52-2 ]
    UniGenei Mm.126525.

    3D structure databases

    ProteinModelPortali Q6NS52.
    SMRi Q6NS52. Positions 10-232, 244-285.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q6NS52.

    Proteomic databases

    PaxDbi Q6NS52.
    PRIDEi Q6NS52.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000040500 ; ENSMUSP00000037900 ; ENSMUSG00000036095 . [Q6NS52-2 ]
    GeneIDi 217480.
    KEGGi mmu:217480.
    UCSCi uc007nkj.1. mouse. [Q6NS52-2 ]
    uc007nkn.1. mouse. [Q6NS52-1 ]

    Organism-specific databases

    CTDi 1607.
    MGIi MGI:2442474. Dgkb.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG47311.
    GeneTreei ENSGT00750000117569.
    HOGENOMi HOG000252931.
    HOVERGENi HBG051345.
    InParanoidi Q6NS52.
    KOi K00901.
    OMAi AQCSEVI.
    OrthoDBi EOG75XGK8.
    PhylomeDBi Q6NS52.
    TreeFami TF313104.

    Enzyme and pathway databases

    Reactomei REACT_219232. Effects of PIP2 hydrolysis.

    Miscellaneous databases

    NextBioi 375875.
    PROi Q6NS52.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6NS52.
    Bgeei Q6NS52.
    CleanExi MM_DGKB.
    Genevestigatori Q6NS52.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Brain cortex.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Eye.
    3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 34-802 (ISOFORM 1).
      Tissue: Brain.

    Entry informationi

    Entry nameiDGKB_MOUSE
    AccessioniPrimary (citable) accession number: Q6NS52
    Secondary accession number(s): Q80TT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3