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Protein

Diacylglycerol kinase beta

Gene

Dgkb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits high phosphorylation activity for long-chain diacylglycerols.By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by phosphatidylserine.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi161 – 1721PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi206 – 2172PROSITE-ProRule annotationAdd BLAST12
Zinc fingeri243 – 293Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri308 – 357Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: InterPro
  • diacylglycerol kinase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-114508. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase beta (EC:2.7.1.107)
Short name:
DAG kinase beta
Alternative name(s):
Diglyceride kinase beta
Short name:
DGK-beta
Gene namesi
Name:Dgkb
Synonyms:Kiaa0718
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2442474. Dgkb.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002646231 – 802Diacylglycerol kinase betaAdd BLAST802

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphothreonineCombined sources1
Modified residuei418PhosphoserineCombined sources1
Modified residuei791PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6NS52.
PeptideAtlasiQ6NS52.
PRIDEiQ6NS52.

PTM databases

iPTMnetiQ6NS52.
PhosphoSitePlusiQ6NS52.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036095.
CleanExiMM_DGKB.
GenevisibleiQ6NS52. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037900.

Structurei

3D structure databases

ProteinModelPortaliQ6NS52.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 183EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini193 – 228EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini432 – 566DAGKcPROSITE-ProRule annotationAdd BLAST135

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri243 – 293Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri308 – 357Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169. Eukaryota.
ENOG410XQVB. LUCA.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiQ6NS52.
KOiK00901.
OMAiCRWCQIT.
OrthoDBiEOG091G04A5.
PhylomeDBiQ6NS52.
TreeFamiTF313104.

Family and domain databases

CDDicd00029. C1. 2 hits.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
SSF47473. SSF47473. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6NS52-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK
60 70 80 90 100
QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS
110 120 130 140 150
KPALLSGGLR MNKGAITPPR SSPANTCSPE VIHLKDIVCY LSLLERGRPE
160 170 180 190 200
DKLEFMFRLY DTDGNGFLDS SELENIIGQM MHVAEYLEWD VTELNPILHE
210 220 230 240 250
MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD DGQHVWRLKH
260 270 280 290 300
FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCVARAP PSCIKTYVKS
310 320 330 340 350
KKNTDVMHHY WVEGNCPTKC DKCHKTVKCY QGLTGLHCVW CQTTLHNKCA
360 370 380 390 400
SHLKPECDCG PLKDHILPPT TICPVVLTMP SAGASVPEER QSTAKKEKSS
410 420 430 440 450
SQQPNKATDK NKMQRANSVT MDGQGLQITP VPGTHPLLVF VNPKSGGKQG
460 470 480 490 500
ERIYRKFQYL LNPRQVYSLS GNGPMPGLHF FRDVPDFRVL ACGGDGTVGW
510 520 530 540 550
ILDCIEKANV VKHPPVAILP LGTGNDLARC LRWGGGYEGE NLMKILKDIE
560 570 580 590 600
SSTEIMLDRW KFEVTPNDKD EKGDPVPYSI INNYFSIGVD ASIAHRFHIM
610 620 630 640 650
REKHPEKFNS RMKNKFWYFE FGTSETFSAT CKKLHESVEI ECDGVQIDLI
660 670 680 690 700
NISLEGIAIL NIPSMHGGSN LWGESKKKRS HRRIEKKGSD KRPTLTDAKE
710 720 730 740 750
LKFASQDLSD QLLEVVGLEG AMEMGQIYTG LKSAGRRLAQ CSSVVIRTSK
760 770 780 790 800
SLPMQIDGEP WMQTPCTIKI THKNQAPMLM GPPPKTGLFC SLIKRTRNRS

KE
Length:802
Mass (Da):90,272
Last modified:December 12, 2006 - v2
Checksum:i2995ECF41057DEF3
GO
Isoform 2 (identifier: Q6NS52-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-56: Missing.

Show »
Length:795
Mass (Da):89,432
Checksum:iD4762491529AB631
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02190050 – 56Missing in isoform 2. 2 Publications7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139408 mRNA. Translation: BAE23997.1.
BC070461 mRNA. Translation: AAH70461.1.
AK122355 mRNA. Translation: BAC65637.1.
CCDSiCCDS25889.1. [Q6NS52-2]
RefSeqiNP_848796.2. NM_178681.4. [Q6NS52-2]
XP_006515132.1. XM_006515069.2. [Q6NS52-1]
XP_006515135.1. XM_006515072.3. [Q6NS52-2]
XP_017170508.1. XM_017315019.1. [Q6NS52-1]
UniGeneiMm.126525.

Genome annotation databases

EnsembliENSMUST00000040500; ENSMUSP00000037900; ENSMUSG00000036095. [Q6NS52-2]
GeneIDi217480.
KEGGimmu:217480.
UCSCiuc007nkj.1. mouse. [Q6NS52-2]
uc007nkn.1. mouse. [Q6NS52-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139408 mRNA. Translation: BAE23997.1.
BC070461 mRNA. Translation: AAH70461.1.
AK122355 mRNA. Translation: BAC65637.1.
CCDSiCCDS25889.1. [Q6NS52-2]
RefSeqiNP_848796.2. NM_178681.4. [Q6NS52-2]
XP_006515132.1. XM_006515069.2. [Q6NS52-1]
XP_006515135.1. XM_006515072.3. [Q6NS52-2]
XP_017170508.1. XM_017315019.1. [Q6NS52-1]
UniGeneiMm.126525.

3D structure databases

ProteinModelPortaliQ6NS52.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037900.

PTM databases

iPTMnetiQ6NS52.
PhosphoSitePlusiQ6NS52.

Proteomic databases

PaxDbiQ6NS52.
PeptideAtlasiQ6NS52.
PRIDEiQ6NS52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040500; ENSMUSP00000037900; ENSMUSG00000036095. [Q6NS52-2]
GeneIDi217480.
KEGGimmu:217480.
UCSCiuc007nkj.1. mouse. [Q6NS52-2]
uc007nkn.1. mouse. [Q6NS52-1]

Organism-specific databases

CTDi1607.
MGIiMGI:2442474. Dgkb.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1169. Eukaryota.
ENOG410XQVB. LUCA.
GeneTreeiENSGT00760000119050.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiQ6NS52.
KOiK00901.
OMAiCRWCQIT.
OrthoDBiEOG091G04A5.
PhylomeDBiQ6NS52.
TreeFamiTF313104.

Enzyme and pathway databases

ReactomeiR-MMU-114508. Effects of PIP2 hydrolysis.

Miscellaneous databases

ChiTaRSiDgkb. mouse.
PROiQ6NS52.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036095.
CleanExiMM_DGKB.
GenevisibleiQ6NS52. MM.

Family and domain databases

CDDicd00029. C1. 2 hits.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
SSF47473. SSF47473. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDGKB_MOUSE
AccessioniPrimary (citable) accession number: Q6NS52
Secondary accession number(s): Q80TT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: November 30, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.