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Protein

Threonylcarbamoyladenosine tRNA methylthiotransferase

Gene

cdkal1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.By similarity

Catalytic activityi

N(6)-L-threonylcarbamoyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-L-threonylcarbamoyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.By similarity

Cofactori

[4Fe-4S] clusterPROSITE-ProRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi105 – 1051Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi134 – 1341Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi210 – 2101Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation
Metal bindingi214 – 2141Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation
Metal bindingi217 – 2171Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Threonylcarbamoyladenosine tRNA methylthiotransferase (EC:2.8.4.5)
Alternative name(s):
CDK5 regulatory subunit-associated protein 1-like 1
tRNA-t(6)A37 methylthiotransferase
Gene namesi
Name:cdkal1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-5895849. cdkal1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei536 – 55621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Threonylcarbamoyladenosine tRNA methylthiotransferasePRO_0000298673Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6NS26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 168109MTTase N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini427 – 48963TRAMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MTTase N-terminal domain.PROSITE-ProRule annotation
Contains 1 TRAM domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG097712.
KOiK15865.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006466. MiaB-like_B.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01578. MiaB-like-B. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NS26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAVCESLLD DIEDMVSATD PKPHDRQSAR KNIVPRARKR NKNNIQEEEP
60 70 80 90 100
PADSTIPGTQ KIWIRTWGCS HNNSDGEYMA GQLAAYGYSI TEQPEKADLW
110 120 130 140 150
LLNSCTVKSP AEDHFRNSIK KAQEANKKVV VSGCVPQAQP RQDYMKGLSI
160 170 180 190 200
IGVQQIDRVV EVVEETIKGH SVRLLGQKKD NGKRLGGARL DLPKIRKNPL
210 220 230 240 250
IEIISINTGC LNACTYCKTK HARGELASYP VEELVDRATQ SFQEGVCEIW
260 270 280 290 300
LTSEDTGAYG RDIGTDLPTL LWKLVEVIPE GAMLRLGMTN PPYILEHLEE
310 320 330 340 350
MAKILNHPRV YAFLHIPVQS ASDSVLMDMK REYCIADFKR VVDFLKERVP
360 370 380 390 400
GITIATDIIC GFPGETDEDF KETLKLVEEY KFPSLFINQF YPRPGTPAAK
410 420 430 440 450
MEQVLAHVKK RRTKELSQLF HSYDPYDHKI GQKQQVLVTE ESFDSQYYVA
460 470 480 490 500
HNRFYEQVLV PKDPDFMGKM VEVKIFEAGK HFMKGQPVQD SYIYTPSITK
510 520 530 540 550
PLAKGEVSGL TEELKPPNNI PKSETLLEKH WERLQVFLFL TALLAAVIAF

VGTKLV
Length:556
Mass (Da):62,906
Last modified:July 5, 2004 - v1
Checksum:i3440758C12E58E4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC070521 mRNA. Translation: AAH70521.1.
RefSeqiNP_001084956.1. NM_001091487.1.
UniGeneiXl.13079.

Genome annotation databases

GeneIDi432014.
KEGGixla:432014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC070521 mRNA. Translation: AAH70521.1.
RefSeqiNP_001084956.1. NM_001091487.1.
UniGeneiXl.13079.

3D structure databases

ProteinModelPortaliQ6NS26.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi432014.
KEGGixla:432014.

Organism-specific databases

CTDi54901.
XenbaseiXB-GENE-5895849. cdkal1.

Phylogenomic databases

HOVERGENiHBG097712.
KOiK15865.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006466. MiaB-like_B.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01578. MiaB-like-B. 1 hit.
TIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDKAL_XENLA
AccessioniPrimary (citable) accession number: Q6NS26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: July 5, 2004
Last modified: October 14, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.