Q6NRE7 (CBLBB_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase CBL-B-B
EC=6.3.2.-

Alternative name(s):
SH3-binding protein CBL-B-B
Signal transduction protein CBL-B-B

Gene names

Name:

cblb-b

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with several SH3 domain-containing proteins and with poly-ubiquitinated proteins By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain. The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme By similarity.
Miscellaneous	This protein has one functional calcium-binding site By similarity.
Sequence similarities	Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Immunity Ubl conjugation pathway
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	Calcium Metal-binding Zinc
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	cell surface receptor signaling pathway Inferred from electronic annotation. Source: InterPro regulation of signaling Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro signal transducer activity Inferred from electronic annotation. Source: InterPro ubiquitin-protein ligase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	E3 ubiquitin-protein ligase CBL-B-B		PRO_0000055864
Regions
	Domain	48 – 356	309	Cbl-PTB
	Calcium binding	232 – 245	14	By similarity
	Zinc finger	386 – 425	40	RING-type
	Region	48 – 180	133	4H
	Region	181 – 253	73	EF-hand-like
	Region	254 – 356	103	SH2-like
	Region	357 – 385	29	Linker
	Compositional bias	489 – 578	90	Pro-rich
	Compositional bias	684 – 687	4	Poly-Pro
Sites
	Binding site	299	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6NRE7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4FFD300CEE119F76	FASTA	764	86,210
	10 20 30 40 50 60 MASSSSSSSN SSTSSSALSG RLPGARSANP RKARILGLFD AIQDAVGPPK QAAADRRTVE 70 80 90 100 110 120 KTWKLMDKVV RLCQNPKLQL KNSPPYILDI LPDTYQHLRL ILSKYDDNQK LAQLSENEYF 130 140 150 160 170 180 KIYIDSLMKK SKRAIRLFKE GKERMYEEQS QERRNLTKLS LIFSHMLAEI KAIFPSGQFQ 190 200 210 220 230 240 GDTFRITKAD AAEFWRKFFG ERTIVPWKIF RQCLHEVQQI SSGLEAMALK STIDLTCNDY 250 260 270 280 290 300 ISVFEFDIFA RLFQPWSSIL RNWNFLAVTH PGYMAFLTYD EVKARLQKYS LKHGSYIFRL 310 320 330 340 350 360 SCTRLGQWAI GYVTAGGNIL QTIPHNKPLF QALIDGSREG FYLYPDGRSY NPDLTDLCEP 370 380 390 400 410 420 TPHDHIKVTQ EQYELYCEMG STFQLCKICA ENDKDVKIEP CGHLMCTSCL TSWQESDGQG 430 440 450 460 470 480 CPFCRCEIKG TEPIIVDPFD PRDENRCCSF NDSLCTPMFD FDDDDLREEC LIMNRLASLR 490 500 510 520 530 540 KMNERQNSPV TSPGSSPLLQ RRKTPPDPVQ IPHLNLPPVP PRLDLIQRGL ARSPCASPTG 550 560 570 580 590 600 SPKSSPCMAR KQDKPLPAPP PPLREPPPPP ERPPPIPPDS RTCRHLHHAE NVPCRDQSTP 610 620 630 640 650 660 NEAWCTRDLS GGNQPSVCRV THDGSPKLGV SSSVLNGRHS RVSTEAGFMR HKHHKRRESP 670 680 690 700 710 720 LETHRVYNGL SGNEEYDVPP RLSPPPPTIT IHPTVICPLL ANSASDKVRN SAEEDDSEYK 730 740 750 760 IPSSHPVSSR LPLHCHSIKH FPRLCENGQC LSNGAHNGIS EIKN

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Oocyte.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC070806 mRNA. Translation: AAH70806.1.
RefSeq	NP_001084790.1. NM_001091321.1.
UniGene	Xl.47173.
3D structure databases
ProteinModelPortal	Q6NRE7.
SMR	Q6NRE7. Positions 48-439.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	431829.
KEGG	xla:431829.
Organism-specific databases
CTD	431829.
Xenbase	XB-GENE-6251770. cblb.
Phylogenomic databases
HOVERGEN	HBG005255.
KO	K04707.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 1.20.930.20. 1 hit. 3.30.40.10. 1 hit. 3.30.505.10. 1 hit.
InterPro	IPR024162. Adaptor_Cbl. IPR014741. Adaptor_Cbl_EF_hand-like. IPR003153. Adaptor_Cbl_N_hlx. IPR014742. Adaptor_Cbl_SH2-like. IPR024159. Cbl_PTB. IPR011992. EF-hand-like_dom. IPR000980. SH2. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
PANTHER	PTHR23007. PTHR23007. 1 hit.
Pfam	PF02262. Cbl_N. 1 hit. PF02761. Cbl_N2. 1 hit. PF02762. Cbl_N3. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00252. SH2. 1 hit. [Graphical view]
SUPFAM	SSF47668. Adaptor_Cbl_N. 1 hit.
PROSITE	PS51506. CBL_PTB. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBLBB_XENLA

Accession

Primary (citable) accession number: Q6NRE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	July 5, 2004
Last modified:	March 6, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program