Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase CBL-B-B

Gene

cblb-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei299PhosphotyrosineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi232 – 245PROSITE-ProRule annotationAdd BLAST14
Zinc fingeri386 – 425RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Immunity, Ubl conjugation pathway

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase CBL-B-B (EC:6.3.2.-)
Alternative name(s):
SH3-binding protein CBL-B-B
Signal transduction protein CBL-B-B
Gene namesi
Name:cblb-b
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6251770. cblb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558641 – 764E3 ubiquitin-protein ligase CBL-B-BAdd BLAST764

Interactioni

Subunit structurei

Interacts with several SH3 domain-containing proteins and with poly-ubiquitinated proteins.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6NRE7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 356Cbl-PTBPROSITE-ProRule annotationAdd BLAST309

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 1804HAdd BLAST133
Regioni181 – 253EF-hand-likeAdd BLAST73
Regioni254 – 356SH2-likeAdd BLAST103
Regioni357 – 385LinkerAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi489 – 578Pro-richAdd BLAST90
Compositional biasi684 – 687Poly-Pro4

Domaini

The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity

Sequence similaritiesi

Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri386 – 425RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG005255.
KOiK04707.

Family and domain databases

CDDicd09920. SH2_Cbl-b_TKB. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.20.930.20. 1 hit.
3.30.40.10. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR024162. Adaptor_Cbl.
IPR014741. Adaptor_Cbl_EF_hand-like.
IPR003153. Adaptor_Cbl_N_hlx.
IPR014742. Adaptor_Cbl_SH2-like.
IPR024159. Cbl_PTB.
IPR011992. EF-hand-dom_pair.
IPR000980. SH2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR23007. PTHR23007. 1 hit.
PfamiPF02262. Cbl_N. 1 hit.
PF02761. Cbl_N2. 1 hit.
PF02762. Cbl_N3. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47668. SSF47668. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS51506. CBL_PTB. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6NRE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSSSSSSN SSTSSSALSG RLPGARSANP RKARILGLFD AIQDAVGPPK
60 70 80 90 100
QAAADRRTVE KTWKLMDKVV RLCQNPKLQL KNSPPYILDI LPDTYQHLRL
110 120 130 140 150
ILSKYDDNQK LAQLSENEYF KIYIDSLMKK SKRAIRLFKE GKERMYEEQS
160 170 180 190 200
QERRNLTKLS LIFSHMLAEI KAIFPSGQFQ GDTFRITKAD AAEFWRKFFG
210 220 230 240 250
ERTIVPWKIF RQCLHEVQQI SSGLEAMALK STIDLTCNDY ISVFEFDIFA
260 270 280 290 300
RLFQPWSSIL RNWNFLAVTH PGYMAFLTYD EVKARLQKYS LKHGSYIFRL
310 320 330 340 350
SCTRLGQWAI GYVTAGGNIL QTIPHNKPLF QALIDGSREG FYLYPDGRSY
360 370 380 390 400
NPDLTDLCEP TPHDHIKVTQ EQYELYCEMG STFQLCKICA ENDKDVKIEP
410 420 430 440 450
CGHLMCTSCL TSWQESDGQG CPFCRCEIKG TEPIIVDPFD PRDENRCCSF
460 470 480 490 500
NDSLCTPMFD FDDDDLREEC LIMNRLASLR KMNERQNSPV TSPGSSPLLQ
510 520 530 540 550
RRKTPPDPVQ IPHLNLPPVP PRLDLIQRGL ARSPCASPTG SPKSSPCMAR
560 570 580 590 600
KQDKPLPAPP PPLREPPPPP ERPPPIPPDS RTCRHLHHAE NVPCRDQSTP
610 620 630 640 650
NEAWCTRDLS GGNQPSVCRV THDGSPKLGV SSSVLNGRHS RVSTEAGFMR
660 670 680 690 700
HKHHKRRESP LETHRVYNGL SGNEEYDVPP RLSPPPPTIT IHPTVICPLL
710 720 730 740 750
ANSASDKVRN SAEEDDSEYK IPSSHPVSSR LPLHCHSIKH FPRLCENGQC
760
LSNGAHNGIS EIKN
Length:764
Mass (Da):86,210
Last modified:July 5, 2004 - v1
Checksum:i4FFD300CEE119F76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC070806 mRNA. Translation: AAH70806.1.
RefSeqiNP_001084790.1. NM_001091321.1.
UniGeneiXl.47173.

Genome annotation databases

GeneIDi431829.
KEGGixla:431829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC070806 mRNA. Translation: AAH70806.1.
RefSeqiNP_001084790.1. NM_001091321.1.
UniGeneiXl.47173.

3D structure databases

ProteinModelPortaliQ6NRE7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi431829.
KEGGixla:431829.

Organism-specific databases

CTDi431829.
XenbaseiXB-GENE-6251770. cblb.

Phylogenomic databases

HOVERGENiHBG005255.
KOiK04707.

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

CDDicd09920. SH2_Cbl-b_TKB. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.20.930.20. 1 hit.
3.30.40.10. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR024162. Adaptor_Cbl.
IPR014741. Adaptor_Cbl_EF_hand-like.
IPR003153. Adaptor_Cbl_N_hlx.
IPR014742. Adaptor_Cbl_SH2-like.
IPR024159. Cbl_PTB.
IPR011992. EF-hand-dom_pair.
IPR000980. SH2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR23007. PTHR23007. 1 hit.
PfamiPF02262. Cbl_N. 1 hit.
PF02761. Cbl_N2. 1 hit.
PF02762. Cbl_N3. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47668. SSF47668. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS51506. CBL_PTB. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBLBB_XENLA
AccessioniPrimary (citable) accession number: Q6NRE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein has one functional calcium-binding site.By similarity

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.